AR016185A1 - Metodo para producir plantas con esterilidad masculina; dichas plantas; celulas vegetales que contienen en su genoma una primera molecula de adn, metodopara producir semillas hibridas, dichas semillas y las plantas logradas de estas, y plantas transgenicas y semillas con las celulas vegetales corres - Google Patents

Metodo para producir plantas con esterilidad masculina; dichas plantas; celulas vegetales que contienen en su genoma una primera molecula de adn, metodopara producir semillas hibridas, dichas semillas y las plantas logradas de estas, y plantas transgenicas y semillas con las celulas vegetales corres

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Publication number
AR016185A1
AR016185A1 ARP990101003A AR016185A1 AR 016185 A1 AR016185 A1 AR 016185A1 AR P990101003 A ARP990101003 A AR P990101003A AR 016185 A1 AR016185 A1 AR 016185A1
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AR
Argentina
Prior art keywords
plants
seeds
rna sequence
plant cells
dna
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English (en)
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Monsanto Technology Llc
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Publication of AR016185A1 publication Critical patent/AR016185A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
    • A01N57/20—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds containing acyclic or cycloaliphatic radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8231—Male-specific, e.g. anther, tapetum, pollen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Botany (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Comprende un método para producir plantas con esterilidad masculina, caracterizada porque comprende los pasos de: insertar en el genoma de una célulavegetal: una primera molécula de ADN que comprende, ligado operativamente en la orientacion 5 a 3; unprimer promotor que funciona en células vegetalescausando la produccion de una primera secuencia de ARN; una primera secuencia de ADN que codifica una proteína que produce tolerancia al glifosato; una primeraregion no traducida 3 que funciona en células vegetales causando la polialdenilacion del extremo 3 de la primera secuencia de ARN; y una segunda molécula deADN que comprende, ligado operativamente en la orientacion 5 a 3; un segundo promotor que funciona en células vegetales causando la produccion de una segundasecuencia de ARN en un tejido reproductor masculino; una segunda secuencia de ADN que codifica una segunda secuencia de ARN que es complementaria de la primerasecuencia de ARN; una segunda region no traducida 3 que funciona encélulas vegetales causando la poliadenilacion del extremo 3 de la segunda secuencia deARN; obtener células vegetales transformadas que contienen la primera y segunda molécula de ADN; regenerrar plantas transformadas a partir de las célulasvegetales transformadas; y exponer las plantas transformadas a glifosato. Las plantas que contienen la primera y la segunda molécula de ADN se vuelvenmasculinos estériles por exposicion a glifosato. Los métodos y las composiciones de la invencion resultan ventajosos para su uso en la generacion de semillashíbridas, para restringir el cruzamiento con especies relacionadas y para prolongar la vida de las flores.
ARP990101003 1998-03-09 1999-03-09 Metodo para producir plantas con esterilidad masculina; dichas plantas; celulas vegetales que contienen en su genoma una primera molecula de adn, metodopara producir semillas hibridas, dichas semillas y las plantas logradas de estas, y plantas transgenicas y semillas con las celulas vegetales corres AR016185A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US7727798P 1998-03-09 1998-03-09

Publications (1)

Publication Number Publication Date
AR016185A1 true AR016185A1 (es) 2001-06-20

Family

ID=22137136

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP990101003 AR016185A1 (es) 1998-03-09 1999-03-09 Metodo para producir plantas con esterilidad masculina; dichas plantas; celulas vegetales que contienen en su genoma una primera molecula de adn, metodopara producir semillas hibridas, dichas semillas y las plantas logradas de estas, y plantas transgenicas y semillas con las celulas vegetales corres

Country Status (6)

Country Link
EP (1) EP1062354A2 (es)
CN (1) CN1300324A (es)
AR (1) AR016185A1 (es)
AU (1) AU2992999A (es)
BR (1) BR9908631A (es)
WO (1) WO1999046396A2 (es)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1301609A2 (en) 2000-07-13 2003-04-16 Pioneer Hi-Bred International, Inc. Zmaxig1-specific polynucleotides and methods of use
EP1174512A1 (en) * 2000-07-17 2002-01-23 Peter Stamp Seed composition and method for reducing or preventing the release of genetically manipulated pollen
US6646186B1 (en) 2000-07-26 2003-11-11 Stine Seed Farm Inc. Hybrid soybeans and methods of production
CA2420406C (en) * 2000-09-29 2014-12-09 Monsanto Technology Llc Glyphosate tolerant wheat plant 33391 and compositions and methods for detection thereof
WO2002045510A2 (en) * 2000-12-07 2002-06-13 D & Pl Technology Holding Corporation Glyphosate-induced male sterility in dicots
DE10163163A1 (de) 2001-12-20 2003-07-03 Basf Ag Verfahren zur Herstellung hochfunktioneller, Hyperverzweigter Polyester durch enzymatische Veresterung
US7230168B2 (en) 2001-12-20 2007-06-12 The Curators Of The University Of Missouri Reversible male sterility in transgenic plants by expression of cytokinin oxidase
ATE497538T1 (de) 2002-02-26 2011-02-15 Syngenta Ltd Verfahren zur selektiven herstellung männlich- oder weiblich-steriler pflanzen
WO2004072235A2 (en) 2003-02-12 2004-08-26 Monsanto Technology Llc Cotton event mon 88913 and compositions and methods for detection thereof
WO2004082366A2 (en) * 2003-03-19 2004-09-30 Regents Of The University Of Minnesota Methods to confer enhanced floral properties to plants
US20060200878A1 (en) 2004-12-21 2006-09-07 Linda Lutfiyya Recombinant DNA constructs and methods for controlling gene expression
EA023885B1 (ru) * 2005-10-13 2016-07-29 МОНСАНТО ТЕКНОЛОДЖИ, ЭлЭлСи Конструкция рекомбинантной днк для индуцирования стерильности в трансгенном растении, стерильные трансгенные растения и способы получения гибридных семян
ZA200803234B (en) * 2005-10-13 2009-01-28 Monsanto Technology Llc Methods for producing hybrid seed
WO2008063755A2 (en) * 2006-10-03 2008-05-29 Monsanto Technology Llc Methods for hybrid corn seed production and compositions produced therefrom
EP2985353A1 (en) 2006-10-12 2016-02-17 Monsanto Technology LLC Plant micrornas and methods of use thereof
WO2011062904A1 (en) 2009-11-23 2011-05-26 Monsanto Technology Llc Transgenic maize event mon 87427 and the relative development scale
CN103635483B (zh) 2011-07-01 2016-11-09 孟山都技术公司 用于选择性调控蛋白质表达的方法和组合物
CN103222423B (zh) * 2013-05-14 2014-11-12 安徽省农业科学院水稻研究所 一种水稻杂交育种材料及其培育方法与应用
AU2016296468B2 (en) 2015-07-22 2020-08-27 Monsanto Technology Llc Methods and compositions for selective regulation of protein expression
CN112080501B (zh) * 2020-09-10 2021-11-23 隆平生物技术(海南)有限公司 重组启动子、基因表达盒及其在植物育种中的应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ227835A (en) * 1988-02-03 1992-09-25 Paladin Hybrids Inc Antisense gene systems of pollination control for hybrid seed production
DE69120419T3 (de) * 1990-08-31 2007-02-15 Monsanto Technology Llc Glyphosattolerante 5-enolpyruvyl-3-phosphoshikimat-synthasen

Also Published As

Publication number Publication date
AU2992999A (en) 1999-09-27
CN1300324A (zh) 2001-06-20
WO1999046396A2 (en) 1999-09-16
BR9908631A (pt) 2000-11-14
EP1062354A2 (en) 2000-12-27
WO1999046396A3 (en) 1999-10-21

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