AR105497A1 - Prueba mejorada de endotoxina bacteriana para la determinación de endotoxinas - Google Patents
Prueba mejorada de endotoxina bacteriana para la determinación de endotoxinasInfo
- Publication number
- AR105497A1 AR105497A1 ARP160102278A ARP160102278A AR105497A1 AR 105497 A1 AR105497 A1 AR 105497A1 AR P160102278 A ARP160102278 A AR P160102278A AR P160102278 A ARP160102278 A AR P160102278A AR 105497 A1 AR105497 A1 AR 105497A1
- Authority
- AR
- Argentina
- Prior art keywords
- sample
- bacterial endotoxin
- antibody
- endotoxin
- determination
- Prior art date
Links
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
- G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- G01N2400/50—Lipopolysaccharides; LPS
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicinal Preparation (AREA)
Abstract
En la presente se informa acerca de un método para determinar una endotoxina bacteriana en concentraciones bajas en un anticuerpo (que ha sido introducido mediante el uso de células bacterianas) que comprende los siguientes pasos en el siguiente orden: i) agregar iones de magnesio a la muestra, ii) diluir la muestra, iii) dializar la muestra con un valor de pH comprendido entre 5,7 y 8,0 contra una solución acuosa libre de endotoxina, iv) determinar la endotoxina bacteriana en la muestra mediante el uso de una prueba de endotoxina bacteriana, en particular el ensayo de lisado de amebocitos de limulus. Reivindicación 2: Método para determinar la endotoxina bacteriana en una muestra que comprende un anticuerpo que exhibe el efecto LER, caracterizado porque el método comprende los siguientes pasos en el siguiente orden: (a) agregado de iones de magnesio, de preferencia en forma de MgCl₂, a la muestra, (b) dilución de la muestra, (c) diálisis de la muestra con un valor de pH de entre 5,7 y 8,0, contra una solución acuosa libre de endotoxina, y (d) determinación de la endotoxina bacteriana en la muestra mediante el uso de un ensayo LAL. Reivindicación 3: Método de acuerdo con la reivindicación 1 ó 2, caracterizado porque el anticuerpo es un anticuerpo terapéutico. Reivindicación 17: Uso de acuerdo con una cualquiera de las reivindicaciones 1 a 16 para dar la muestra que comprende un anticuerpo que reacciona ante el factor C en la cascada enzimática LAL.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP15178683.7A EP3124976B1 (en) | 2015-07-28 | 2015-07-28 | Improved bacterial endotoxin test for the determination of endotoxins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR105497A1 true AR105497A1 (es) | 2017-10-11 |
Family
ID=53776384
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP160102278A AR105497A1 (es) | 2015-07-28 | 2016-07-27 | Prueba mejorada de endotoxina bacteriana para la determinación de endotoxinas |
Country Status (21)
| Country | Link |
|---|---|
| US (2) | US10585097B2 (es) |
| EP (1) | EP3124976B1 (es) |
| JP (2) | JP6800226B2 (es) |
| KR (1) | KR102630300B1 (es) |
| CN (2) | CN111638346A (es) |
| AR (1) | AR105497A1 (es) |
| AU (1) | AU2016298598B2 (es) |
| BR (1) | BR112018001748A8 (es) |
| CA (1) | CA2989960C (es) |
| DK (1) | DK3124976T3 (es) |
| ES (1) | ES2699526T3 (es) |
| HR (1) | HRP20181954T1 (es) |
| IL (2) | IL256660B (es) |
| LT (1) | LT3124976T (es) |
| MX (1) | MX374236B (es) |
| PL (1) | PL3124976T3 (es) |
| PT (1) | PT3124976T (es) |
| RS (1) | RS58167B1 (es) |
| SI (1) | SI3124976T1 (es) |
| TR (1) | TR201816369T4 (es) |
| WO (1) | WO2017017135A1 (es) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11099189B2 (en) | 2017-10-17 | 2021-08-24 | Board Of Regents, The University Of Texas System | Limulus amoebocyte lysate assay and method of same |
| EP3483609B1 (en) * | 2017-11-09 | 2022-01-05 | Andreas Buchberger | Method and kit for sample preparation and endotoxin determination |
| CN109613228A (zh) * | 2018-12-20 | 2019-04-12 | 湛江安度斯生物有限公司 | 一种减少或消除氢氧化铝佐剂干扰细菌内毒素检测的方法 |
| CN111141909B (zh) * | 2019-12-24 | 2023-05-30 | 南京健友生化制药股份有限公司 | 一种凝胶法检测硫代甘油中细菌内毒素的方法 |
| US11965888B2 (en) * | 2020-04-09 | 2024-04-23 | DePuy Synthes Products, Inc. | Quantifying endotoxin load in bacterial biofilms |
| CN112462015B (zh) * | 2020-11-18 | 2022-07-12 | 海南倍特药业有限公司 | 一种氢溴酸瑞马唑仑细菌内毒素的检测方法 |
| CN116242977A (zh) * | 2022-12-14 | 2023-06-09 | 无锡药明生物技术股份有限公司 | 内毒素保留时间的估算方法及其应用 |
| CN116609521B (zh) * | 2023-01-17 | 2025-12-02 | 河北省药品医疗器械检验研究院(河北省化妆品检验研究中心) | 重组乙型肝炎疫苗细菌内毒素快速检测试剂盒及其使用方法 |
| CN116396916B (zh) * | 2023-03-02 | 2023-12-01 | 广州普言生物科技有限公司 | 低内毒素大肠杆菌及低内毒素重组人源胶原蛋白大肠杆菌工程菌 |
| CN117969228B (zh) * | 2024-04-02 | 2024-05-28 | 成都翼泰生物科技有限公司 | 一种菌液稀释液、制备方法、参考品、试剂盒及其应用 |
| CN120594816B (zh) * | 2025-06-20 | 2025-11-25 | 北京全式金生物技术股份有限公司 | 一种重组c因子法检测内毒素的反应缓冲液及其应用 |
Family Cites Families (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2123198A (en) * | 1936-10-23 | 1938-07-12 | Lederle Lab Inc | Treatment of antitoxins and the like |
| US4096091A (en) * | 1974-07-17 | 1978-06-20 | Baxter Travenol Laboratories, Inc. | Limulus lysate having improved capacity to precipitate in the presence of low endotoxin concentrations, and reconstituting solutions therefor |
| JPS5549791Y2 (es) * | 1976-04-21 | 1980-11-20 | ||
| EP0107688B1 (en) * | 1982-04-28 | 1986-10-01 | The Trustees Of The Garfield Weston Trust For Research Into Heart Surgery | Processes for the production of blood products |
| US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
| DK255887D0 (da) * | 1987-05-20 | 1987-05-20 | Claus Koch | Immunoassay |
| US5959177A (en) | 1989-10-27 | 1999-09-28 | The Scripps Research Institute | Transgenic plants expressing assembled secretory antibodies |
| JP2957251B2 (ja) | 1990-09-28 | 1999-10-04 | 生化学工業株式会社 | エンドトキシンの測定法 |
| GB9021370D0 (en) * | 1990-10-02 | 1990-11-14 | Ciba Geigy Ag | Monoclonal antibodies directed against complexes formed by thrombin and thrombin inhibitors |
| WO1993006217A1 (en) | 1991-09-19 | 1993-04-01 | Genentech, Inc. | EXPRESSION IN E. COLI OF ANTIBODY FRAGMENTS HAVING AT LEAST A CYSTEINE PRESENT AS A FREE THIOL, USE FOR THE PRODUCTION OF BIFUNCTIONAL F(ab')2 ANTIBODIES |
| JP2761839B2 (ja) * | 1993-12-28 | 1998-06-04 | 株式会社 ソフィア | 遊技機の表示制御装置 |
| US5789199A (en) | 1994-11-03 | 1998-08-04 | Genentech, Inc. | Process for bacterial production of polypeptides |
| US5840523A (en) | 1995-03-01 | 1998-11-24 | Genetech, Inc. | Methods and compositions for secretion of heterologous polypeptides |
| JPH09171018A (ja) * | 1995-12-19 | 1997-06-30 | Tokuyama Corp | 免疫学的凝集反応試薬の製造方法 |
| EP0892271A1 (en) * | 1997-07-14 | 1999-01-20 | Universiteit Gent Laboratorium voor Bromatologie Faculteit Farmaceutische Wetenschappen | Detection of mycotoxins by flow-through membrane-based enzyme immunoassay |
| US6040498A (en) | 1998-08-11 | 2000-03-21 | North Caroline State University | Genetically engineered duckweed |
| JP2000105232A (ja) * | 1998-09-29 | 2000-04-11 | Sekisui Chem Co Ltd | 抗ＨＢｓ抗体測定試薬の製造方法 |
| JP4310854B2 (ja) | 1999-06-14 | 2009-08-12 | Ｊｓｒ株式会社 | 診断薬用担体粒子および診断薬 |
| US7125978B1 (en) | 1999-10-04 | 2006-10-24 | Medicago Inc. | Promoter for regulating expression of foreign genes |
| CN100427603C (zh) | 1999-10-04 | 2008-10-22 | 麦迪卡格公司 | 调控外源基因转录的方法 |
| AU2001296301A1 (en) * | 2000-09-26 | 2002-04-08 | Human Genome Sciences, Inc. | Nucleic acids, proteins, and antibodies |
| AU2001292273B2 (en) | 2000-09-27 | 2007-11-15 | Japan Tobacco Inc. | Novel proteins, genes encoding them and method of using the same |
| DE102005002969A1 (de) * | 2005-01-21 | 2006-08-03 | Profos Ag | Verfahren zum Nachweis und zur Entfernung von Endotoxin |
| PE20140196A1 (es) | 2007-08-09 | 2014-03-19 | Boehringer Ingelheim Int | Anticuerpos anti-cd37 |
| WO2009063840A1 (ja) * | 2007-11-12 | 2009-05-22 | Hiroshima University | エンドトキシンの濃度測定方法および濃度測定用キット |
| JP2008275638A (ja) * | 2008-06-23 | 2008-11-13 | Daicen Membrane Systems Ltd | エンドトキシン濃度の簡易測定器 |
| US7846678B2 (en) * | 2008-08-18 | 2010-12-07 | BioDtech, Inc. | Enhancing endotoxin detection |
| AR078161A1 (es) * | 2009-09-11 | 2011-10-19 | Hoffmann La Roche | Formulaciones farmaceuticas muy concentradas de un anticuerpo anti cd20. uso de la formulacion. metodo de tratamiento. |
| CN102841205A (zh) * | 2011-07-19 | 2012-12-26 | 辽宁思百得医药科技有限公司 | 一种检测注射用氟罗沙星细菌内毒素的方法 |
| WO2013032601A1 (en) | 2011-09-01 | 2013-03-07 | Fresenius Medical Care Holdings, Inc. | Kit for sampling and detection of endotoxin in aqueous solution |
| CN102901726B (zh) * | 2012-09-14 | 2014-08-13 | 莫水晶 | 血液细菌内毒素检测试剂盒的制备和应用 |
-
2015
- 2015-07-28 DK DK15178683.7T patent/DK3124976T3/en active
- 2015-07-28 PT PT15178683T patent/PT3124976T/pt unknown
- 2015-07-28 SI SI201530465T patent/SI3124976T1/sl unknown
- 2015-07-28 ES ES15178683T patent/ES2699526T3/es active Active
- 2015-07-28 TR TR2018/16369T patent/TR201816369T4/tr unknown
- 2015-07-28 PL PL15178683T patent/PL3124976T3/pl unknown
- 2015-07-28 LT LTEP15178683.7T patent/LT3124976T/lt unknown
- 2015-07-28 EP EP15178683.7A patent/EP3124976B1/en active Active
- 2015-07-28 RS RS20181468A patent/RS58167B1/sr unknown
-
2016
- 2016-07-27 KR KR1020187006090A patent/KR102630300B1/ko active Active
- 2016-07-27 WO PCT/EP2016/067896 patent/WO2017017135A1/en not_active Ceased
- 2016-07-27 CN CN202010521942.XA patent/CN111638346A/zh active Pending
- 2016-07-27 CN CN201680044156.7A patent/CN108027374B/zh active Active
- 2016-07-27 AR ARP160102278A patent/AR105497A1/es active IP Right Grant
- 2016-07-27 AU AU2016298598A patent/AU2016298598B2/en active Active
- 2016-07-27 MX MX2018000679A patent/MX374236B/es active IP Right Grant
- 2016-07-27 US US15/739,503 patent/US10585097B2/en active Active
- 2016-07-27 JP JP2018524545A patent/JP6800226B2/ja active Active
- 2016-07-27 CA CA2989960A patent/CA2989960C/en active Active
- 2016-07-27 BR BR112018001748A patent/BR112018001748A8/pt not_active Application Discontinuation
-
2017
- 2017-12-31 IL IL256660A patent/IL256660B/en active IP Right Grant
-
2018
- 2018-11-21 HR HRP20181954TT patent/HRP20181954T1/hr unknown
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2020
- 2020-01-28 US US16/774,461 patent/US11360085B2/en active Active
- 2020-05-11 IL IL274576A patent/IL274576B/en active IP Right Grant
- 2020-11-24 JP JP2020194005A patent/JP7164790B2/ja active Active
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| FG | Grant, registration |