CA2521497C - Modulation de l'activite des cytokinines dans les plantes - Google Patents

Modulation de l'activite des cytokinines dans les plantes Download PDF

Info

Publication number: CA2521497C
Authority: CA; Canada
Prior art keywords: plant; promoter; expression; cytokinin; seed
Prior art date: 2003-04-04
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Fee Related

Application number

CA2521497A

Other languages

English (en)

Other versions

CA2521497A1 (fr

Inventor

Jeffrey E. Habben

Chris Zinselmeier

Dwight T. Tomes

Shane E. Abbitt

Timothy G. Helentjaris

Xiaomu Niu

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Pioneer Hi Bred International Inc

Original Assignee

Pioneer Hi Bred International Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2003-04-04

Filing date

2004-04-02

Publication date

2012-11-27

2004-04-02 Application filed by Pioneer Hi Bred International Inc filed Critical Pioneer Hi Bred International Inc

2004-10-21 Publication of CA2521497A1 publication Critical patent/CA2521497A1/fr

2012-11-27 Application granted granted Critical

2012-11-27 Publication of CA2521497C publication Critical patent/CA2521497C/fr

2024-04-02 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

Links

UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 title claims abstract description 205
239000004062 cytokinin Substances 0.000 title claims abstract description 205
230000000694 effects Effects 0.000 title description 63
108090000623 proteins and genes Proteins 0.000 claims abstract description 245
230000014509 gene expression Effects 0.000 claims abstract description 196
238000000034 method Methods 0.000 claims abstract description 175
230000009261 transgenic effect Effects 0.000 claims abstract description 123
230000001965 increasing effect Effects 0.000 claims abstract description 74
230000001105 regulatory effect Effects 0.000 claims abstract description 50
230000001850 reproductive effect Effects 0.000 claims abstract description 29
230000001131 transforming effect Effects 0.000 claims abstract description 15
230000001172 regenerating effect Effects 0.000 claims abstract description 7
241000196324 Embryophyta Species 0.000 claims description 491
102000040430 polynucleotide Human genes 0.000 claims description 153
108091033319 polynucleotide Proteins 0.000 claims description 153
239000002157 polynucleotide Substances 0.000 claims description 152
240000008042 Zea mays Species 0.000 claims description 129
235000002017 Zea mays subsp mays Nutrition 0.000 claims description 110
235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 95
235000009973 maize Nutrition 0.000 claims description 95
230000009466 transformation Effects 0.000 claims description 68
102000004169 proteins and genes Human genes 0.000 claims description 61
239000002245 particle Substances 0.000 claims description 45
239000003623 enhancer Substances 0.000 claims description 36
230000002068 genetic effect Effects 0.000 claims description 36
241000589158 Agrobacterium Species 0.000 claims description 29
108091026890 Coding region Proteins 0.000 claims description 29
108010050516 adenylate isopentenyltransferase Proteins 0.000 claims description 29
244000068988 Glycine max Species 0.000 claims description 27
235000010469 Glycine max Nutrition 0.000 claims description 25
230000010152 pollination Effects 0.000 claims description 24
230000001404 mediated effect Effects 0.000 claims description 22
238000004519 manufacturing process Methods 0.000 claims description 20
230000008117 seed development Effects 0.000 claims description 18
238000011161 development Methods 0.000 claims description 17
230000002829 reductive effect Effects 0.000 claims description 17
241000701489 Cauliflower mosaic virus Species 0.000 claims description 16
230000000692 anti-sense effect Effects 0.000 claims description 15
230000001627 detrimental effect Effects 0.000 claims description 15
230000018109 developmental process Effects 0.000 claims description 15
108020004511 Recombinant DNA Proteins 0.000 claims description 13
230000001488 breeding effect Effects 0.000 claims description 13
230000021749 root development Effects 0.000 claims description 13
108700028146 Genetic Enhancer Elements Proteins 0.000 claims description 12
230000000442 meristematic effect Effects 0.000 claims description 12
239000002028 Biomass Substances 0.000 claims description 11
101710118613 DNA polymerase sliding clamp 2 Proteins 0.000 claims description 11
230000002123 temporal effect Effects 0.000 claims description 11
230000001594 aberrant effect Effects 0.000 claims description 9
230000036579 abiotic stress Effects 0.000 claims description 7
238000004520 electroporation Methods 0.000 claims description 7
230000008641 drought stress Effects 0.000 claims description 6
238000000520 microinjection Methods 0.000 claims description 5
101100342815 Caenorhabditis elegans lec-1 gene Proteins 0.000 claims description 4
101000882584 Homo sapiens Estrogen receptor Proteins 0.000 claims description 4
239000000835 fiber Substances 0.000 claims description 4
230000000306 recurrent effect Effects 0.000 claims description 4
XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 3
229910052710 silicon Inorganic materials 0.000 claims description 3
239000010703 silicon Substances 0.000 claims description 3
125000003729 nucleotide group Chemical group 0.000 abstract description 57
239000002773 nucleotide Substances 0.000 abstract description 56
108091028043 Nucleic acid sequence Proteins 0.000 abstract description 36
239000000203 mixture Substances 0.000 abstract description 23
230000001976 improved effect Effects 0.000 abstract description 13
239000003153 chemical reaction reagent Substances 0.000 abstract description 12
230000007613 environmental effect Effects 0.000 abstract description 11
230000003247 decreasing effect Effects 0.000 abstract description 6
206010000210 abortion Diseases 0.000 abstract description 5
231100000176 abortion Toxicity 0.000 abstract description 5
108090000765 processed proteins & peptides Proteins 0.000 description 180
210000004027 cell Anatomy 0.000 description 171
102000004196 processed proteins & peptides Human genes 0.000 description 170
229920001184 polypeptide Polymers 0.000 description 164
108020004414 DNA Proteins 0.000 description 128
210000001519 tissue Anatomy 0.000 description 119
108090000790 Enzymes Proteins 0.000 description 108
102000004190 Enzymes Human genes 0.000 description 106
229940088598 enzyme Drugs 0.000 description 106
101150012864 ipt gene Proteins 0.000 description 96
239000013598 vector Substances 0.000 description 78
101100457842 Mus musculus Trit1 gene Proteins 0.000 description 69
101100397103 Rhodococcus fascians fas4 gene Proteins 0.000 description 69
101150000417 izt gene Proteins 0.000 description 69
239000012634 fragment Substances 0.000 description 67
210000002257 embryonic structure Anatomy 0.000 description 60
239000013615 primer Substances 0.000 description 57
239000013612 plasmid Substances 0.000 description 55
235000018102 proteins Nutrition 0.000 description 55
230000027455 binding Effects 0.000 description 44
230000001851 biosynthetic effect Effects 0.000 description 43
150000007523 nucleic acids Chemical class 0.000 description 42
239000002609 medium Substances 0.000 description 41
238000003752 polymerase chain reaction Methods 0.000 description 40
102000039446 nucleic acids Human genes 0.000 description 37
108020004707 nucleic acids Proteins 0.000 description 37
239000003550 marker Substances 0.000 description 36
108091032973 (ribonucleotides)n+m Proteins 0.000 description 32
239000000047 product Substances 0.000 description 28
230000004048 modification Effects 0.000 description 27
238000012986 modification Methods 0.000 description 27
235000001014 amino acid Nutrition 0.000 description 26
230000015572 biosynthetic process Effects 0.000 description 25
238000009396 hybridization Methods 0.000 description 25
239000000523 sample Substances 0.000 description 25
238000004458 analytical method Methods 0.000 description 24
210000001161 mammalian embryo Anatomy 0.000 description 24
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 23
229940024606 amino acid Drugs 0.000 description 23
108010088245 cytokinin oxidase Proteins 0.000 description 23
125000003275 alpha amino acid group Chemical group 0.000 description 22
150000001413 amino acids Chemical class 0.000 description 22
108700019146 Transgenes Proteins 0.000 description 21
230000035897 transcription Effects 0.000 description 21
238000013518 transcription Methods 0.000 description 21
239000002585 base Substances 0.000 description 20
230000000408 embryogenic effect Effects 0.000 description 20
230000012010 growth Effects 0.000 description 20
238000002955 isolation Methods 0.000 description 20
108700039691 Genetic Promoter Regions Proteins 0.000 description 18
238000003556 assay Methods 0.000 description 18
238000012217 deletion Methods 0.000 description 18
230000037430 deletion Effects 0.000 description 18
210000005069 ears Anatomy 0.000 description 18
230000002503 metabolic effect Effects 0.000 description 18
230000035882 stress Effects 0.000 description 17
235000002637 Nicotiana tabacum Nutrition 0.000 description 16
244000061176 Nicotiana tabacum Species 0.000 description 16
108091034117 Oligonucleotide Proteins 0.000 description 16
230000008929 regeneration Effects 0.000 description 16
238000011069 regeneration method Methods 0.000 description 16
238000006467 substitution reaction Methods 0.000 description 16
235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 15
235000005822 corn Nutrition 0.000 description 15
230000008569 process Effects 0.000 description 15
230000014616 translation Effects 0.000 description 15
241000589155 Agrobacterium tumefaciens Species 0.000 description 14
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 14
238000007792 addition Methods 0.000 description 14
238000005516 engineering process Methods 0.000 description 14
230000001939 inductive effect Effects 0.000 description 14
238000013519 translation Methods 0.000 description 14
238000011144 upstream manufacturing Methods 0.000 description 14
108010000700 Acetolactate synthase Proteins 0.000 description 13
206010020649 Hyperkeratosis Diseases 0.000 description 13
235000013339 cereals Nutrition 0.000 description 13
239000002299 complementary DNA Substances 0.000 description 13
238000005755 formation reaction Methods 0.000 description 13
239000007788 liquid Substances 0.000 description 13
108020004999 messenger RNA Proteins 0.000 description 13
239000000725 suspension Substances 0.000 description 13
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 12
102000053602 DNA Human genes 0.000 description 12
239000005557 antagonist Substances 0.000 description 12
239000003795 chemical substances by application Substances 0.000 description 12
230000035772 mutation Effects 0.000 description 12
230000004044 response Effects 0.000 description 12
125000000539 amino acid group Chemical group 0.000 description 11
230000000295 complement effect Effects 0.000 description 11
230000000875 corresponding effect Effects 0.000 description 11
210000000056 organ Anatomy 0.000 description 11
230000010076 replication Effects 0.000 description 11
239000000243 solution Substances 0.000 description 11
239000000126 substance Substances 0.000 description 11
240000005979 Hordeum vulgare Species 0.000 description 10
240000006394 Sorghum bicolor Species 0.000 description 10
241000700605 Viruses Species 0.000 description 10
235000007244 Zea mays Nutrition 0.000 description 10
239000000556 agonist Substances 0.000 description 10
238000009395 breeding Methods 0.000 description 10
238000010367 cloning Methods 0.000 description 10
238000010276 construction Methods 0.000 description 10
239000004009 herbicide Substances 0.000 description 10
UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 10
241000894006 Bacteria Species 0.000 description 9
240000000385 Brassica napus var. napus Species 0.000 description 9
235000007340 Hordeum vulgare Nutrition 0.000 description 9
230000007423 decrease Effects 0.000 description 9
239000013604 expression vector Substances 0.000 description 9
230000002363 herbicidal effect Effects 0.000 description 9
230000000977 initiatory effect Effects 0.000 description 9
239000007787 solid Substances 0.000 description 9
230000000392 somatic effect Effects 0.000 description 9
UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 9
229940023877 zeatin Drugs 0.000 description 9
235000014698 Brassica juncea var multisecta Nutrition 0.000 description 8
235000006008 Brassica napus var napus Nutrition 0.000 description 8
235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 8
235000004977 Brassica sinapistrum Nutrition 0.000 description 8
241000588724 Escherichia coli Species 0.000 description 8
241000238631 Hexapoda Species 0.000 description 8
235000011684 Sorghum saccharatum Nutrition 0.000 description 8
108010050181 aleurone Proteins 0.000 description 8
238000013459 approach Methods 0.000 description 8
230000001580 bacterial effect Effects 0.000 description 8
230000003115 biocidal effect Effects 0.000 description 8
238000006243 chemical reaction Methods 0.000 description 8
238000001514 detection method Methods 0.000 description 8
230000006870 function Effects 0.000 description 8
238000003780 insertion Methods 0.000 description 8
230000037431 insertion Effects 0.000 description 8
239000000463 material Substances 0.000 description 8
230000008774 maternal effect Effects 0.000 description 8
238000002844 melting Methods 0.000 description 8
230000008018 melting Effects 0.000 description 8
238000010369 molecular cloning Methods 0.000 description 8
238000012545 processing Methods 0.000 description 8
238000004114 suspension culture Methods 0.000 description 8
230000002103 transcriptional effect Effects 0.000 description 8
108020004491 Antisense DNA Proteins 0.000 description 7
244000020551 Helianthus annuus Species 0.000 description 7
235000003222 Helianthus annuus Nutrition 0.000 description 7
241000282414 Homo sapiens Species 0.000 description 7
241000219823 Medicago Species 0.000 description 7
235000007238 Secale cereale Nutrition 0.000 description 7
235000002595 Solanum tuberosum Nutrition 0.000 description 7
244000061456 Solanum tuberosum Species 0.000 description 7
229940100389 Sulfonylurea Drugs 0.000 description 7
108020004566 Transfer RNA Proteins 0.000 description 7
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 7
239000003816 antisense DNA Substances 0.000 description 7
230000001925 catabolic effect Effects 0.000 description 7
230000001413 cellular effect Effects 0.000 description 7
238000012512 characterization method Methods 0.000 description 7
210000000349 chromosome Anatomy 0.000 description 7
150000001875 compounds Chemical class 0.000 description 7
-1 diHZ Chemical compound 0.000 description 7
210000003527 eukaryotic cell Anatomy 0.000 description 7
238000002474 experimental method Methods 0.000 description 7
PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 7
229910052737 gold Inorganic materials 0.000 description 7
239000010931 gold Substances 0.000 description 7
108010082527 phosphinothricin N-acetyltransferase Proteins 0.000 description 7
238000002360 preparation method Methods 0.000 description 7
210000001938 protoplast Anatomy 0.000 description 7
238000011160 research Methods 0.000 description 7
108091008146 restriction endonucleases Proteins 0.000 description 7
238000012216 screening Methods 0.000 description 7
241000894007 species Species 0.000 description 7
239000000758 substrate Substances 0.000 description 7
YROXIXLRRCOBKF-UHFFFAOYSA-N sulfonylurea Chemical class OC(=N)N=S(=O)=O YROXIXLRRCOBKF-UHFFFAOYSA-N 0.000 description 7
230000006878 vivipary Effects 0.000 description 7
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
102000040650 (ribonucleotides)n+m Human genes 0.000 description 6
108091027551 Cointegrate Proteins 0.000 description 6
108091029865 Exogenous DNA Proteins 0.000 description 6
241001465754 Metazoa Species 0.000 description 6
240000007594 Oryza sativa Species 0.000 description 6
235000007164 Oryza sativa Nutrition 0.000 description 6
108700008625 Reporter Genes Proteins 0.000 description 6
241000209056 Secale Species 0.000 description 6
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
229930006000 Sucrose Natural products 0.000 description 6
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 6
241000209140 Triticum Species 0.000 description 6
235000021307 Triticum Nutrition 0.000 description 6
230000033228 biological regulation Effects 0.000 description 6
230000001276 controlling effect Effects 0.000 description 6
108020001507 fusion proteins Proteins 0.000 description 6
102000037865 fusion proteins Human genes 0.000 description 6
230000006698 induction Effects 0.000 description 6
239000003112 inhibitor Substances 0.000 description 6
239000003446 ligand Substances 0.000 description 6
230000013011 mating Effects 0.000 description 6
229910052757 nitrogen Inorganic materials 0.000 description 6
239000002243 precursor Substances 0.000 description 6
230000000644 propagated effect Effects 0.000 description 6
238000000746 purification Methods 0.000 description 6
238000012340 reverse transcriptase PCR Methods 0.000 description 6
150000003839 salts Chemical class 0.000 description 6
238000012163 sequencing technique Methods 0.000 description 6
ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 description 6
238000010561 standard procedure Methods 0.000 description 6
239000005720 sucrose Substances 0.000 description 6
239000006228 supernatant Substances 0.000 description 6
238000003786 synthesis reaction Methods 0.000 description 6
229940088594 vitamin Drugs 0.000 description 6
239000011782 vitamin Substances 0.000 description 6
235000013343 vitamin Nutrition 0.000 description 6
229930003231 vitamin Natural products 0.000 description 6
244000099147 Ananas comosus Species 0.000 description 5
241000219194 Arabidopsis Species 0.000 description 5
244000105624 Arachis hypogaea Species 0.000 description 5
241000207199 Citrus Species 0.000 description 5
244000299507 Gossypium hirsutum Species 0.000 description 5
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 5
240000003183 Manihot esculenta Species 0.000 description 5
235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 5
229920002472 Starch Polymers 0.000 description 5
108091081024 Start codon Proteins 0.000 description 5
229920002494 Zein Polymers 0.000 description 5
108010055615 Zein Proteins 0.000 description 5
239000000427 antigen Substances 0.000 description 5
108091007433 antigens Proteins 0.000 description 5
102000036639 antigens Human genes 0.000 description 5
230000004071 biological effect Effects 0.000 description 5
230000008859 change Effects 0.000 description 5
235000020971 citrus fruits Nutrition 0.000 description 5
239000003184 complementary RNA Substances 0.000 description 5
239000000499 gel Substances 0.000 description 5
230000035784 germination Effects 0.000 description 5
SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 5
238000012423 maintenance Methods 0.000 description 5
101150113864 pat gene Proteins 0.000 description 5
210000002706 plastid Anatomy 0.000 description 5
230000004481 post-translational protein modification Effects 0.000 description 5
238000003259 recombinant expression Methods 0.000 description 5
235000009566 rice Nutrition 0.000 description 5
150000003384 small molecules Chemical class 0.000 description 5
239000002689 soil Substances 0.000 description 5
238000000527 sonication Methods 0.000 description 5
235000019698 starch Nutrition 0.000 description 5
239000008107 starch Substances 0.000 description 5
238000001890 transfection Methods 0.000 description 5
239000005019 zein Substances 0.000 description 5
229940093612 zein Drugs 0.000 description 5
235000007119 Ananas comosus Nutrition 0.000 description 4
108020005544 Antisense RNA Proteins 0.000 description 4
102100026189 Beta-galactosidase Human genes 0.000 description 4
HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
235000013162 Cocos nucifera Nutrition 0.000 description 4
244000060011 Cocos nucifera Species 0.000 description 4
102000012410 DNA Ligases Human genes 0.000 description 4
108010061982 DNA Ligases Proteins 0.000 description 4
238000001712 DNA sequencing Methods 0.000 description 4
208000035240 Disease Resistance Diseases 0.000 description 4
229920002148 Gellan gum Polymers 0.000 description 4
101000765010 Homo sapiens Beta-galactosidase Proteins 0.000 description 4
108091026898 Leader sequence (mRNA) Proteins 0.000 description 4
241000209510 Liliopsida Species 0.000 description 4
239000004472 Lysine Substances 0.000 description 4
241000829100 Macaca mulatta polyomavirus 1 Species 0.000 description 4
235000014826 Mangifera indica Nutrition 0.000 description 4
240000007228 Mangifera indica Species 0.000 description 4
240000007817 Olea europaea Species 0.000 description 4
108700026244 Open Reading Frames Proteins 0.000 description 4
229910019142 PO4 Inorganic materials 0.000 description 4
244000025272 Persea americana Species 0.000 description 4
235000008673 Persea americana Nutrition 0.000 description 4
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
240000004808 Saccharomyces cerevisiae Species 0.000 description 4
238000012300 Sequence Analysis Methods 0.000 description 4
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 4
244000269722 Thea sinensis Species 0.000 description 4
244000299461 Theobroma cacao Species 0.000 description 4
235000009470 Theobroma cacao Nutrition 0.000 description 4
241000723873 Tobacco mosaic virus Species 0.000 description 4
108700009124 Transcription Initiation Site Proteins 0.000 description 4
108091023040 Transcription factor Proteins 0.000 description 4
239000002253 acid Substances 0.000 description 4
230000009418 agronomic effect Effects 0.000 description 4
230000004075 alteration Effects 0.000 description 4
239000003242 anti bacterial agent Substances 0.000 description 4
GINJFDRNADDBIN-FXQIFTODSA-N bilanafos Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCP(C)(O)=O GINJFDRNADDBIN-FXQIFTODSA-N 0.000 description 4
230000008827 biological function Effects 0.000 description 4
230000015556 catabolic process Effects 0.000 description 4
229930002875 chlorophyll Natural products 0.000 description 4
235000019804 chlorophyll Nutrition 0.000 description 4
ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 4
238000006731 degradation reaction Methods 0.000 description 4
239000012153 distilled water Substances 0.000 description 4
238000009826 distribution Methods 0.000 description 4
235000013399 edible fruits Nutrition 0.000 description 4
241001233957 eudicotyledons Species 0.000 description 4
230000002349 favourable effect Effects 0.000 description 4
230000030279 gene silencing Effects 0.000 description 4
238000012226 gene silencing method Methods 0.000 description 4
238000010353 genetic engineering Methods 0.000 description 4
230000013595 glycosylation Effects 0.000 description 4
238000006206 glycosylation reaction Methods 0.000 description 4
239000001307 helium Substances 0.000 description 4
229910052734 helium Inorganic materials 0.000 description 4
SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 4
238000001727 in vivo Methods 0.000 description 4
208000015181 infectious disease Diseases 0.000 description 4
230000003993 interaction Effects 0.000 description 4
230000014634 leaf senescence Effects 0.000 description 4
229920005610 lignin Polymers 0.000 description 4
150000002632 lipids Chemical class 0.000 description 4
239000012528 membrane Substances 0.000 description 4
108010058731 nopaline synthase Proteins 0.000 description 4
235000015097 nutrients Nutrition 0.000 description 4
239000003921 oil Substances 0.000 description 4
210000001672 ovary Anatomy 0.000 description 4
235000021317 phosphate Nutrition 0.000 description 4
238000003976 plant breeding Methods 0.000 description 4
238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 4
229920001223 polyethylene glycol Polymers 0.000 description 4
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 4
238000002741 site-directed mutagenesis Methods 0.000 description 4
230000008961 swelling Effects 0.000 description 4
WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 4
229910052721 tungsten Inorganic materials 0.000 description 4
239000010937 tungsten Substances 0.000 description 4
238000012795 verification Methods 0.000 description 4
239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 3
101100148680 Arabidopsis thaliana SAG12 gene Proteins 0.000 description 3
229930192334 Auxin Natural products 0.000 description 3
244000075850 Avena orientalis Species 0.000 description 3
108091003079 Bovine Serum Albumin Proteins 0.000 description 3
240000002791 Brassica napus Species 0.000 description 3
235000011293 Brassica napus Nutrition 0.000 description 3
102000014914 Carrier Proteins Human genes 0.000 description 3
235000003255 Carthamus tinctorius Nutrition 0.000 description 3
244000020518 Carthamus tinctorius Species 0.000 description 3
108020004705 Codon Proteins 0.000 description 3
108020004635 Complementary DNA Proteins 0.000 description 3
229920000742 Cotton Polymers 0.000 description 3
YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 3
241000206602 Eukaryota Species 0.000 description 3
241000218218 Ficus <angiosperm> Species 0.000 description 3
102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 3
108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 3
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 3
241000227653 Lycopersicon Species 0.000 description 3
241000699666 Mus <mouse, genus> Species 0.000 description 3
241000234295 Musa Species 0.000 description 3
206010028980 Neoplasm Diseases 0.000 description 3
238000000636 Northern blotting Methods 0.000 description 3
IAJOBQBIJHVGMQ-UHFFFAOYSA-N Phosphinothricin Natural products CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 3
241000508269 Psidium Species 0.000 description 3
108020004682 Single-Stranded DNA Proteins 0.000 description 3
235000007230 Sorghum bicolor Nutrition 0.000 description 3
238000002105 Southern blotting Methods 0.000 description 3
108700026226 TATA Box Proteins 0.000 description 3
102000040945 Transcription factor Human genes 0.000 description 3
230000001133 acceleration Effects 0.000 description 3
230000009471 action Effects 0.000 description 3
OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 3
239000002363 auxin Substances 0.000 description 3
229940098773 bovine serum albumin Drugs 0.000 description 3
239000000872 buffer Substances 0.000 description 3
238000004113 cell culture Methods 0.000 description 3
238000003776 cleavage reaction Methods 0.000 description 3
230000021615 conjugation Effects 0.000 description 3
230000001419 dependent effect Effects 0.000 description 3
230000029087 digestion Effects 0.000 description 3
235000021186 dishes Nutrition 0.000 description 3
230000001747 exhibiting effect Effects 0.000 description 3
230000004927 fusion Effects 0.000 description 3
IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 description 3
210000004408 hybridoma Anatomy 0.000 description 3
230000002163 immunogen Effects 0.000 description 3
230000006872 improvement Effects 0.000 description 3
238000000338 in vitro Methods 0.000 description 3
230000005764 inhibitory process Effects 0.000 description 3
230000010354 integration Effects 0.000 description 3
230000035800 maturation Effects 0.000 description 3
230000037353 metabolic pathway Effects 0.000 description 3
229930182817 methionine Natural products 0.000 description 3
238000002703 mutagenesis Methods 0.000 description 3
231100000350 mutagenesis Toxicity 0.000 description 3
230000002018 overexpression Effects 0.000 description 3
238000010422 painting Methods 0.000 description 3
239000000123 paper Substances 0.000 description 3
230000037361 pathway Effects 0.000 description 3
239000008188 pellet Substances 0.000 description 3
230000008447 perception Effects 0.000 description 3
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
239000010452 phosphate Substances 0.000 description 3
230000008635 plant growth Effects 0.000 description 3
239000005014 poly(hydroxyalkanoate) Substances 0.000 description 3
229920000903 polyhydroxyalkanoate Polymers 0.000 description 3
229920000642 polymer Polymers 0.000 description 3
230000009467 reduction Effects 0.000 description 3
230000000284 resting effect Effects 0.000 description 3
238000007894 restriction fragment length polymorphism technique Methods 0.000 description 3
230000000717 retained effect Effects 0.000 description 3
230000007017 scission Effects 0.000 description 3
230000003248 secreting effect Effects 0.000 description 3
239000006152 selective media Substances 0.000 description 3
230000009758 senescence Effects 0.000 description 3
230000035939 shock Effects 0.000 description 3
239000011780 sodium chloride Substances 0.000 description 3
229940063673 spermidine Drugs 0.000 description 3
238000012360 testing method Methods 0.000 description 3
238000012546 transfer Methods 0.000 description 3
241001515965 unidentified phage Species 0.000 description 3
230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 3
239000013603 viral vector Substances 0.000 description 3
230000003612 virological effect Effects 0.000 description 3
OVSKIKFHRZPJSS-DOMIDYPGSA-N 2-(2,4-dichlorophenoxy)acetic acid Chemical compound OC(=O)[14CH2]OC1=CC=C(Cl)C=C1Cl OVSKIKFHRZPJSS-DOMIDYPGSA-N 0.000 description 2
101710168820 2S seed storage albumin protein Proteins 0.000 description 2
108010020183 3-phosphoshikimate 1-carboxyvinyltransferase Proteins 0.000 description 2
FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
USVMJSALORZVDV-UHFFFAOYSA-N 6-(gamma,gamma-dimethylallylamino)purine riboside Natural products C1=NC=2C(NCC=C(C)C)=NC=NC=2N1C1OC(CO)C(O)C1O USVMJSALORZVDV-UHFFFAOYSA-N 0.000 description 2
KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
GOSWTRUMMSCNCW-HNNGNKQASA-N 9-ribosyl-trans-zeatin Chemical compound C1=NC=2C(NC\C=C(CO)/C)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O GOSWTRUMMSCNCW-HNNGNKQASA-N 0.000 description 2
230000005730 ADP ribosylation Effects 0.000 description 2
241000589156 Agrobacterium rhizogenes Species 0.000 description 2
235000010777 Arachis hypogaea Nutrition 0.000 description 2
DNUKXVMPARLPFN-XUXIUFHCSA-N Arg-Leu-Ile Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O DNUKXVMPARLPFN-XUXIUFHCSA-N 0.000 description 2
235000007319 Avena orientalis Nutrition 0.000 description 2
241000193388 Bacillus thuringiensis Species 0.000 description 2
235000011331 Brassica Nutrition 0.000 description 2
241000219198 Brassica Species 0.000 description 2
240000008100 Brassica rapa Species 0.000 description 2
235000011292 Brassica rapa Nutrition 0.000 description 2
235000004936 Bromus mango Nutrition 0.000 description 2
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
239000005496 Chlorsulfuron Substances 0.000 description 2
229940122644 Chymotrypsin inhibitor Drugs 0.000 description 2
101710137926 Chymotrypsin inhibitor Proteins 0.000 description 2
108091033380 Coding strand Proteins 0.000 description 2
241000218631 Coniferophyta Species 0.000 description 2
108010066133 D-octopine dehydrogenase Proteins 0.000 description 2
239000003155 DNA primer Substances 0.000 description 2
230000006820 DNA synthesis Effects 0.000 description 2
241000255581 Drosophila <fruit fly, genus> Species 0.000 description 2
238000002965 ELISA Methods 0.000 description 2
238000012286 ELISA Assay Methods 0.000 description 2
101710186901 Globulin 1 Proteins 0.000 description 2
WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
239000005562 Glyphosate Substances 0.000 description 2
235000009432 Gossypium hirsutum Nutrition 0.000 description 2
NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 2
108010093488 His-His-His-His-His-His Proteins 0.000 description 2
108010001336 Horseradish Peroxidase Proteins 0.000 description 2
108091092195 Intron Proteins 0.000 description 2
241000207783 Ipomoea Species 0.000 description 2
235000021506 Ipomoea Nutrition 0.000 description 2
244000017020 Ipomoea batatas Species 0.000 description 2
235000002678 Ipomoea batatas Nutrition 0.000 description 2
ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 2
DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 2
108090001090 Lectins Proteins 0.000 description 2
102000004856 Lectins Human genes 0.000 description 2
102000003960 Ligases Human genes 0.000 description 2
108090000364 Ligases Proteins 0.000 description 2
108060001084 Luciferase Proteins 0.000 description 2
239000005089 Luciferase Substances 0.000 description 2
241000721701 Lynx Species 0.000 description 2
241000710118 Maize chlorotic mottle virus Species 0.000 description 2
241000723994 Maize dwarf mosaic virus Species 0.000 description 2
235000004456 Manihot esculenta Nutrition 0.000 description 2
235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 2
240000005561 Musa balbisiana Species 0.000 description 2
235000018290 Musa x paradisiaca Nutrition 0.000 description 2
USVMJSALORZVDV-SDBHATRESA-N N(6)-(Delta(2)-isopentenyl)adenosine Chemical compound C1=NC=2C(NCC=C(C)C)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O USVMJSALORZVDV-SDBHATRESA-N 0.000 description 2
DUISZFLWBAPRBR-SDBHATRESA-N N(6)-(dimethylallyl)adenosine 5'-monophosphate Chemical compound C1=NC=2C(NCC=C(C)C)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O DUISZFLWBAPRBR-SDBHATRESA-N 0.000 description 2
NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 2
101710196810 Non-specific lipid-transfer protein 2 Proteins 0.000 description 2
108020004711 Nucleic Acid Probes Proteins 0.000 description 2
108091005461 Nucleic proteins Proteins 0.000 description 2
235000002725 Olea europaea Nutrition 0.000 description 2
241000209094 Oryza Species 0.000 description 2
108020002230 Pancreatic Ribonuclease Proteins 0.000 description 2
102000005891 Pancreatic ribonuclease Human genes 0.000 description 2
102000003992 Peroxidases Human genes 0.000 description 2
108091000080 Phosphotransferase Proteins 0.000 description 2
239000002202 Polyethylene glycol Substances 0.000 description 2
239000004793 Polystyrene Substances 0.000 description 2
240000001679 Psidium guajava Species 0.000 description 2
235000013929 Psidium pyriferum Nutrition 0.000 description 2
238000012181 QIAquick gel extraction kit Methods 0.000 description 2
238000012228 RNA interference-mediated gene silencing Methods 0.000 description 2
108020005091 Replication Origin Proteins 0.000 description 2
102000006382 Ribonucleases Human genes 0.000 description 2
108010083644 Ribonucleases Proteins 0.000 description 2
108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 2
244000082988 Secale cereale Species 0.000 description 2
108010016634 Seed Storage Proteins Proteins 0.000 description 2
235000009184 Spondias indica Nutrition 0.000 description 2
NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
235000006468 Thea sinensis Nutrition 0.000 description 2
JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 2
241000723792 Tobacco etch virus Species 0.000 description 2
244000098338 Triticum aestivum Species 0.000 description 2
108090000848 Ubiquitin Proteins 0.000 description 2
102000044159 Ubiquitin Human genes 0.000 description 2
230000002159 abnormal effect Effects 0.000 description 2
238000009825 accumulation Methods 0.000 description 2
108091000039 acetoacetyl-CoA reductase Proteins 0.000 description 2
150000007513 acids Chemical class 0.000 description 2
239000011543 agarose gel Substances 0.000 description 2
238000005054 agglomeration Methods 0.000 description 2
230000002776 aggregation Effects 0.000 description 2
239000003570 air Substances 0.000 description 2
230000003321 amplification Effects 0.000 description 2
238000000540 analysis of variance Methods 0.000 description 2
229930002877 anthocyanin Natural products 0.000 description 2
235000010208 anthocyanin Nutrition 0.000 description 2
239000004410 anthocyanin Substances 0.000 description 2
150000004636 anthocyanins Chemical class 0.000 description 2
230000000890 antigenic effect Effects 0.000 description 2
230000000680 avirulence Effects 0.000 description 2
229940097012 bacillus thuringiensis Drugs 0.000 description 2
101150103518 bar gene Proteins 0.000 description 2
230000008901 benefit Effects 0.000 description 2
108091008324 binding proteins Proteins 0.000 description 2
229920000704 biodegradable plastic Polymers 0.000 description 2
230000005540 biological transmission Effects 0.000 description 2
238000006664 bond formation reaction Methods 0.000 description 2
239000001110 calcium chloride Substances 0.000 description 2
229910001628 calcium chloride Inorganic materials 0.000 description 2
235000011148 calcium chloride Nutrition 0.000 description 2
150000001720 carbohydrates Chemical class 0.000 description 2
235000014633 carbohydrates Nutrition 0.000 description 2
229910052799 carbon Inorganic materials 0.000 description 2
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
230000032823 cell division Effects 0.000 description 2
210000000170 cell membrane Anatomy 0.000 description 2
230000036755 cellular response Effects 0.000 description 2
238000005119 centrifugation Methods 0.000 description 2
VJYIFXVZLXQVHO-UHFFFAOYSA-N chlorsulfuron Chemical compound COC1=NC(C)=NC(NC(=O)NS(=O)(=O)C=2C(=CC=CC=2)Cl)=N1 VJYIFXVZLXQVHO-UHFFFAOYSA-N 0.000 description 2
230000002759 chromosomal effect Effects 0.000 description 2
239000003541 chymotrypsin inhibitor Substances 0.000 description 2
238000004590 computer program Methods 0.000 description 2
238000012258 culturing Methods 0.000 description 2
230000000593 degrading effect Effects 0.000 description 2
238000013461 design Methods 0.000 description 2
238000001784 detoxification Methods 0.000 description 2
201000010099 disease Diseases 0.000 description 2
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
230000003828 downregulation Effects 0.000 description 2
230000013020 embryo development Effects 0.000 description 2
210000002472 endoplasmic reticulum Anatomy 0.000 description 2
230000002708 enhancing effect Effects 0.000 description 2
230000006353 environmental stress Effects 0.000 description 2
238000011156 evaluation Methods 0.000 description 2
108010052305 exodeoxyribonuclease III Proteins 0.000 description 2
239000000284 extract Substances 0.000 description 2
108010060641 flavanone synthetase Proteins 0.000 description 2
230000008124 floral development Effects 0.000 description 2
238000009472 formulation Methods 0.000 description 2
239000012737 fresh medium Substances 0.000 description 2
230000002538 fungal effect Effects 0.000 description 2
230000006251 gamma-carboxylation Effects 0.000 description 2
238000001502 gel electrophoresis Methods 0.000 description 2
238000010359 gene isolation Methods 0.000 description 2
230000009368 gene silencing by RNA Effects 0.000 description 2
238000012248 genetic selection Methods 0.000 description 2
239000012869 germination medium Substances 0.000 description 2
239000011521 glass Substances 0.000 description 2
101150091511 glb-1 gene Proteins 0.000 description 2
XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 2
229940097068 glyphosate Drugs 0.000 description 2
238000000227 grinding Methods 0.000 description 2
239000001963 growth medium Substances 0.000 description 2
238000002744 homologous recombination Methods 0.000 description 2
230000006801 homologous recombination Effects 0.000 description 2
239000005556 hormone Substances 0.000 description 2
229940088597 hormone Drugs 0.000 description 2
125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
230000033444 hydroxylation Effects 0.000 description 2
238000005805 hydroxylation reaction Methods 0.000 description 2
108010002685 hygromycin-B kinase Proteins 0.000 description 2
238000003119 immunoblot Methods 0.000 description 2
238000002347 injection Methods 0.000 description 2
239000007924 injection Substances 0.000 description 2
229960000367 inositol Drugs 0.000 description 2
CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 2
238000006192 iodination reaction Methods 0.000 description 2
230000002262 irrigation Effects 0.000 description 2
238000003973 irrigation Methods 0.000 description 2
229960000318 kanamycin Drugs 0.000 description 2
229930027917 kanamycin Natural products 0.000 description 2
SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
229930182823 kanamycin A Natural products 0.000 description 2
108010045069 keyhole-limpet hemocyanin Proteins 0.000 description 2
239000002523 lectin Substances 0.000 description 2
238000009630 liquid culture Methods 0.000 description 2
238000011068 loading method Methods 0.000 description 2
239000011159 matrix material Substances 0.000 description 2
238000005259 measurement Methods 0.000 description 2
230000007246 mechanism Effects 0.000 description 2
230000037230 mobility Effects 0.000 description 2
238000001823 molecular biology technique Methods 0.000 description 2
230000001338 necrotic effect Effects 0.000 description 2
238000003199 nucleic acid amplification method Methods 0.000 description 2
239000002853 nucleic acid probe Substances 0.000 description 2
230000003647 oxidation Effects 0.000 description 2
238000007254 oxidation reaction Methods 0.000 description 2
235000020232 peanut Nutrition 0.000 description 2
108040007629 peroxidase activity proteins Proteins 0.000 description 2
230000009120 phenotypic response Effects 0.000 description 2
102000020233 phosphotransferase Human genes 0.000 description 2
230000010399 physical interaction Effects 0.000 description 2
229930195732 phytohormone Natural products 0.000 description 2
230000008121 plant development Effects 0.000 description 2
230000037039 plant physiology Effects 0.000 description 2
239000013600 plasmid vector Substances 0.000 description 2
102000054765 polymorphisms of proteins Human genes 0.000 description 2
229920002223 polystyrene Polymers 0.000 description 2
230000001124 posttranscriptional effect Effects 0.000 description 2
210000001236 prokaryotic cell Anatomy 0.000 description 2
229960002429 proline Drugs 0.000 description 2
230000002797 proteolythic effect Effects 0.000 description 2
238000012552 review Methods 0.000 description 2
230000005070 ripening Effects 0.000 description 2
210000003296 saliva Anatomy 0.000 description 2
CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 2
230000028327 secretion Effects 0.000 description 2
238000000926 separation method Methods 0.000 description 2
230000001568 sexual effect Effects 0.000 description 2
229910001415 sodium ion Inorganic materials 0.000 description 2
229960000268 spectinomycin Drugs 0.000 description 2
UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 2
UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
125000001424 substituent group Chemical group 0.000 description 2
230000019635 sulfation Effects 0.000 description 2
238000005670 sulfation reaction Methods 0.000 description 2
229910052717 sulfur Inorganic materials 0.000 description 2
239000011593 sulfur Substances 0.000 description 2
230000000153 supplemental effect Effects 0.000 description 2
230000001629 suppression Effects 0.000 description 2
230000004083 survival effect Effects 0.000 description 2
235000019157 thiamine Nutrition 0.000 description 2
KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 2
229960003495 thiamine Drugs 0.000 description 2
239000011721 thiamine Substances 0.000 description 2
GOSWTRUMMSCNCW-UHFFFAOYSA-N trans-zeatin riboside Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1OC(CO)C(O)C1O GOSWTRUMMSCNCW-UHFFFAOYSA-N 0.000 description 2
230000005026 transcription initiation Effects 0.000 description 2
230000005030 transcription termination Effects 0.000 description 2
238000010361 transduction Methods 0.000 description 2
230000026683 transduction Effects 0.000 description 2
238000000844 transformation Methods 0.000 description 2
230000001052 transient effect Effects 0.000 description 2
238000010798 ubiquitination Methods 0.000 description 2
230000034512 ubiquitination Effects 0.000 description 2
241000701161 unidentified adenovirus Species 0.000 description 2
241000701447 unidentified baculovirus Species 0.000 description 2
230000003827 upregulation Effects 0.000 description 2
235000013311 vegetables Nutrition 0.000 description 2
210000005253 yeast cell Anatomy 0.000 description 2
VYRAJOITMBSQSE-UHFFFAOYSA-N zeatin-9-glucoside Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1OC(CO)C(O)C(O)C1O VYRAJOITMBSQSE-UHFFFAOYSA-N 0.000 description 2
DIGQNXIGRZPYDK-WKSCXVIASA-N (2R)-6-amino-2-[[2-[[(2S)-2-[[2-[[(2R)-2-[[(2S)-2-[[(2R,3S)-2-[[2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S,3S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2R)-2-[[2-[[2-[[2-[(2-amino-1-hydroxyethylidene)amino]-3-carboxy-1-hydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxypropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1,5-dihydroxy-5-iminopentylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxybutylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1,3-dihydroxypropylidene]amino]-1-hydroxyethylidene]amino]-1-hydroxy-3-sulfanylpropylidene]amino]-1-hydroxyethylidene]amino]hexanoic acid Chemical compound C[C@@H]([C@@H](C(=N[C@@H](CS)C(=N[C@@H](C)C(=N[C@@H](CO)C(=NCC(=N[C@@H](CCC(=N)O)C(=NC(CS)C(=N[C@H]([C@H](C)O)C(=N[C@H](CS)C(=N[C@H](CO)C(=NCC(=N[C@H](CS)C(=NCC(=N[C@H](CCCCN)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N=C([C@H](CS)N=C([C@H](CO)N=C([C@H](CO)N=C([C@H](C)N=C(CN=C([C@H](CO)N=C([C@H](CS)N=C(CN=C(C(CS)N=C(C(CC(=O)O)N=C(CN)O)O)O)O)O)O)O)O)O)O)O)O DIGQNXIGRZPYDK-WKSCXVIASA-N 0.000 description 1
WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 1
RMVYFKFMCYZXTA-UHFFFAOYSA-N 2-[(1-carboxy-3-methylbutyl)amino]pentanedioic acid Chemical compound CC(C)CC(C(O)=O)NC(C(O)=O)CCC(O)=O RMVYFKFMCYZXTA-UHFFFAOYSA-N 0.000 description 1
ZBMRKNMTMPPMMK-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid;azane Chemical compound [NH4+].CP(O)(=O)CCC(N)C([O-])=O ZBMRKNMTMPPMMK-UHFFFAOYSA-N 0.000 description 1
LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
UPMXNNIRAGDFEH-UHFFFAOYSA-N 3,5-dibromo-4-hydroxybenzonitrile Chemical compound OC1=C(Br)C=C(C#N)C=C1Br UPMXNNIRAGDFEH-UHFFFAOYSA-N 0.000 description 1
HCWLJSDMOMMDRF-SZWOQXJISA-N 3-[(3s,6r)-2-oxo-6-[(1s,2r,3r)-1,2,3,4-tetrahydroxybutyl]morpholin-3-yl]propanamide Chemical compound NC(=O)CC[C@@H]1NC[C@H]([C@@H](O)[C@H](O)[C@H](O)CO)OC1=O HCWLJSDMOMMDRF-SZWOQXJISA-N 0.000 description 1
102100026105 3-ketoacyl-CoA thiolase, mitochondrial Human genes 0.000 description 1
ODHCTXKNWHHXJC-VKHMYHEASA-N 5-oxo-L-proline Chemical compound OC(=O)[C@@H]1CCC(=O)N1 ODHCTXKNWHHXJC-VKHMYHEASA-N 0.000 description 1
FVFVNNKYKYZTJU-UHFFFAOYSA-N 6-chloro-1,3,5-triazine-2,4-diamine Chemical group NC1=NC(N)=NC(Cl)=N1 FVFVNNKYKYZTJU-UHFFFAOYSA-N 0.000 description 1
QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
WFPZSXYXPSUOPY-ROYWQJLOSA-N ADP alpha-D-glucoside Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H]1O)O)N1C=2N=CN=C(C=2N=C1)N)OP(O)(=O)OP(O)(=O)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O WFPZSXYXPSUOPY-ROYWQJLOSA-N 0.000 description 1
108010003902 Acetyl-CoA C-acyltransferase Proteins 0.000 description 1
101710146995 Acyl carrier protein Proteins 0.000 description 1
229920001817 Agar Polymers 0.000 description 1
229920000936 Agarose Polymers 0.000 description 1
ILQOASSPNGAIBC-UHFFFAOYSA-N Agropine Natural products OC(O)C(O)CC(O)C1CN2C(CCC2=O)C(=O)O1 ILQOASSPNGAIBC-UHFFFAOYSA-N 0.000 description 1
OMMDTNGURYRDAC-NRPADANISA-N Ala-Glu-Val Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C(C)C)C(O)=O OMMDTNGURYRDAC-NRPADANISA-N 0.000 description 1
GRPHQEMIFDPKOE-HGNGGELXSA-N Ala-His-Glu Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CCC(O)=O)C(O)=O GRPHQEMIFDPKOE-HGNGGELXSA-N 0.000 description 1
PNALXAODQKTNLV-JBDRJPRFSA-N Ala-Ile-Ala Chemical compound C[C@H](N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(O)=O PNALXAODQKTNLV-JBDRJPRFSA-N 0.000 description 1
PIXQDIGKDNNOOV-GUBZILKMSA-N Ala-Lys-Gln Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(N)=O)C(O)=O PIXQDIGKDNNOOV-GUBZILKMSA-N 0.000 description 1
SUHLZMHFRALVSY-YUMQZZPRSA-N Ala-Lys-Gly Chemical compound NCCCC[C@H](NC(=O)[C@@H](N)C)C(=O)NCC(O)=O SUHLZMHFRALVSY-YUMQZZPRSA-N 0.000 description 1
108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
102000007698 Alcohol dehydrogenase Human genes 0.000 description 1
241000724328 Alfalfa mosaic virus Species 0.000 description 1
244000291564 Allium cepa Species 0.000 description 1
235000002732 Allium cepa var. cepa Nutrition 0.000 description 1
241000207875 Antirrhinum Species 0.000 description 1
240000001436 Antirrhinum majus Species 0.000 description 1
108700007114 Arabidopsis AGAMOUS Proteins 0.000 description 1
101100269449 Arabidopsis thaliana AHK4 gene Proteins 0.000 description 1
101100269450 Arabidopsis thaliana AHK5 gene Proteins 0.000 description 1
101100274513 Arabidopsis thaliana CKL10 gene Proteins 0.000 description 1
101100188552 Arabidopsis thaliana OCT3 gene Proteins 0.000 description 1
235000003911 Arachis Nutrition 0.000 description 1
AWMAZIIEFPFHCP-RCWTZXSCSA-N Arg-Pro-Thr Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)O)C(O)=O AWMAZIIEFPFHCP-RCWTZXSCSA-N 0.000 description 1
LLQIAIUAKGNOSE-NHCYSSNCSA-N Arg-Val-Gln Chemical compound NC(=O)CC[C@@H](C(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](N)CCCN=C(N)N LLQIAIUAKGNOSE-NHCYSSNCSA-N 0.000 description 1
DBWYWXNMZZYIRY-LPEHRKFASA-N Asp-Arg-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(=O)O)N)C(=O)O DBWYWXNMZZYIRY-LPEHRKFASA-N 0.000 description 1
DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
235000005340 Asparagus officinalis Nutrition 0.000 description 1
241000228212 Aspergillus Species 0.000 description 1
241001106067 Atropa Species 0.000 description 1
235000005781 Avena Nutrition 0.000 description 1
KHBQMWCZKVMBLN-UHFFFAOYSA-N Benzenesulfonamide Chemical compound NS(=O)(=O)C1=CC=CC=C1 KHBQMWCZKVMBLN-UHFFFAOYSA-N 0.000 description 1
241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 1
108010006654 Bleomycin Proteins 0.000 description 1
241000283690 Bos taurus Species 0.000 description 1
235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 1
240000003259 Brassica oleracea var. botrytis Species 0.000 description 1
241000724256 Brome mosaic virus Species 0.000 description 1
239000005489 Bromoxynil Substances 0.000 description 1
241000209200 Bromus Species 0.000 description 1
239000002126 C01EB10 - Adenosine Substances 0.000 description 1
101100268056 Caenorhabditis elegans zag-1 gene Proteins 0.000 description 1
241000209507 Camellia Species 0.000 description 1
241000283707 Capra Species 0.000 description 1
235000002566 Capsicum Nutrition 0.000 description 1
240000008574 Capsicum frutescens Species 0.000 description 1
101710132601 Capsid protein Proteins 0.000 description 1
108010078791 Carrier Proteins Proteins 0.000 description 1
241000700199 Cavia porcellus Species 0.000 description 1
108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 1
108091062157 Cis-regulatory element Proteins 0.000 description 1
101710094648 Coat protein Proteins 0.000 description 1
241000737241 Cocos Species 0.000 description 1
108020004394 Complementary RNA Proteins 0.000 description 1
108091035707 Consensus sequence Proteins 0.000 description 1
241000699802 Cricetulus griseus Species 0.000 description 1
101000622007 Crotalus adamanteus Snake venom metalloproteinase adamalysin-2 Proteins 0.000 description 1
MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 1
235000010071 Cucumis prophetarum Nutrition 0.000 description 1
244000024469 Cucumis prophetarum Species 0.000 description 1
240000008067 Cucumis sativus Species 0.000 description 1
235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
241000219122 Cucurbita Species 0.000 description 1
BMHBJCVEXUBGFI-BIIVOSGPSA-N Cys-Cys-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CS)NC(=O)[C@H](CS)N)C(=O)O BMHBJCVEXUBGFI-BIIVOSGPSA-N 0.000 description 1
241000701022 Cytomegalovirus Species 0.000 description 1
NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 1
YAHZABJORDUQGO-NQXXGFSBSA-N D-ribulose 1,5-bisphosphate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)C(=O)COP(O)(O)=O YAHZABJORDUQGO-NQXXGFSBSA-N 0.000 description 1
230000004544 DNA amplification Effects 0.000 description 1
238000007399 DNA isolation Methods 0.000 description 1
239000003298 DNA probe Substances 0.000 description 1
101710096830 DNA-3-methyladenine glycosylase Proteins 0.000 description 1
102100039128 DNA-3-methyladenine glycosylase Human genes 0.000 description 1
102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
241000289763 Dasygaster padockina Species 0.000 description 1
241000208296 Datura Species 0.000 description 1
241000208175 Daucus Species 0.000 description 1
229920002307 Dextran Polymers 0.000 description 1
101100125027 Dictyostelium discoideum mhsp70 gene Proteins 0.000 description 1
108010082495 Dietary Plant Proteins Proteins 0.000 description 1
240000001879 Digitalis lutea Species 0.000 description 1
241001057636 Dracaena deremensis Species 0.000 description 1
101100457919 Drosophila melanogaster stg gene Proteins 0.000 description 1
101150111720 EPSPS gene Proteins 0.000 description 1
241000710188 Encephalomyocarditis virus Species 0.000 description 1
102100031780 Endonuclease Human genes 0.000 description 1
241000588698 Erwinia Species 0.000 description 1
241000392944 Erwinia soli Species 0.000 description 1
241000701959 Escherichia virus Lambda Species 0.000 description 1
108060002716 Exonuclease Proteins 0.000 description 1
108010087894 Fatty acid desaturases Proteins 0.000 description 1
241000282326 Felis catus Species 0.000 description 1
241000701484 Figwort mosaic virus Species 0.000 description 1
241000700662 Fowlpox virus Species 0.000 description 1
241000220223 Fragaria Species 0.000 description 1
241000233866 Fungi Species 0.000 description 1
101150035751 GSP2 gene Proteins 0.000 description 1
108010093031 Galactosidases Proteins 0.000 description 1
102000002464 Galactosidases Human genes 0.000 description 1
241000287828 Gallus gallus Species 0.000 description 1
241000702463 Geminiviridae Species 0.000 description 1
229930182566 Gentamicin Natural products 0.000 description 1
CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
241000208152 Geranium Species 0.000 description 1
102000006395 Globulins Human genes 0.000 description 1
108010044091 Globulins Proteins 0.000 description 1
229920001503 Glucan Polymers 0.000 description 1
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
102000053187 Glucuronidase Human genes 0.000 description 1
108010060309 Glucuronidase Proteins 0.000 description 1
KKBWDNZXYLGJEY-UHFFFAOYSA-N Gly-Arg-Pro Natural products NCC(=O)NC(CCNC(=N)N)C(=O)N1CCCC1C(=O)O KKBWDNZXYLGJEY-UHFFFAOYSA-N 0.000 description 1
AAHSHTLISQUZJL-QSFUFRPTSA-N Gly-Ile-Ile Chemical compound [H]NCC(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O AAHSHTLISQUZJL-QSFUFRPTSA-N 0.000 description 1
LRQXRHGQEVWGPV-NHCYSSNCSA-N Gly-Leu-Ile Chemical compound CC[C@H](C)[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)CN LRQXRHGQEVWGPV-NHCYSSNCSA-N 0.000 description 1
SOEGEPHNZOISMT-BYPYZUCNSA-N Gly-Ser-Gly Chemical compound NCC(=O)N[C@@H](CO)C(=O)NCC(O)=O SOEGEPHNZOISMT-BYPYZUCNSA-N 0.000 description 1
VNNRLUNBJSWZPF-ZKWXMUAHSA-N Gly-Ser-Ile Chemical compound [H]NCC(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O VNNRLUNBJSWZPF-ZKWXMUAHSA-N 0.000 description 1
239000004471 Glycine Substances 0.000 description 1
102100021181 Golgi phosphoprotein 3 Human genes 0.000 description 1
241000219146 Gossypium Species 0.000 description 1
235000009438 Gossypium Nutrition 0.000 description 1
HVLSXIKZNLPZJJ-TXZCQADKSA-N HA peptide Chemical compound C([C@@H](C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](C)C(O)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](N)CC=1C=CC(O)=CC=1)C1=CC=C(O)C=C1 HVLSXIKZNLPZJJ-TXZCQADKSA-N 0.000 description 1
101150031823 HSP70 gene Proteins 0.000 description 1
108010004889 Heat-Shock Proteins Proteins 0.000 description 1
102000002812 Heat-Shock Proteins Human genes 0.000 description 1
241000208818 Helianthus Species 0.000 description 1
101710154606 Hemagglutinin Proteins 0.000 description 1
WMKXFMUJRCEGRP-SRVKXCTJSA-N His-Asn-His Chemical compound C1=C(NC=N1)C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CC2=CN=CN2)C(=O)O)N WMKXFMUJRCEGRP-SRVKXCTJSA-N 0.000 description 1
108700005087 Homeobox Genes Proteins 0.000 description 1
241000282412 Homo Species 0.000 description 1
101000899240 Homo sapiens Endoplasmic reticulum chaperone BiP Proteins 0.000 description 1
101000582989 Homo sapiens Phospholipid phosphatase-related protein type 4 Proteins 0.000 description 1
241000209219 Hordeum Species 0.000 description 1
108700032155 Hordeum vulgare hordothionin Proteins 0.000 description 1
241000208278 Hyoscyamus Species 0.000 description 1
102000017727 Immunoglobulin Variable Region Human genes 0.000 description 1
108010067060 Immunoglobulin Variable Region Proteins 0.000 description 1
206010021929 Infertility male Diseases 0.000 description 1
229930010555 Inosine Natural products 0.000 description 1
UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
235000013757 Juglans Nutrition 0.000 description 1
241000758789 Juglans Species 0.000 description 1
108010025815 Kanamycin Kinase Proteins 0.000 description 1
LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 description 1
SENJXOPIZNYLHU-UHFFFAOYSA-N L-leucyl-L-arginine Natural products CC(C)CC(N)C(=O)NC(C(O)=O)CCCN=C(N)N SENJXOPIZNYLHU-UHFFFAOYSA-N 0.000 description 1
FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
229930182821 L-proline Natural products 0.000 description 1
101150058089 LTP2 gene Proteins 0.000 description 1
241000208822 Lactuca Species 0.000 description 1
MJOZZTKJZQFKDK-GUBZILKMSA-N Leu-Ala-Gln Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@H](C(O)=O)CCC(N)=O MJOZZTKJZQFKDK-GUBZILKMSA-N 0.000 description 1
NEEOBPIXKWSBRF-IUCAKERBSA-N Leu-Glu-Gly Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(O)=O NEEOBPIXKWSBRF-IUCAKERBSA-N 0.000 description 1
LVTJJOJKDCVZGP-QWRGUYRKSA-N Leu-Lys-Gly Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)NCC(O)=O LVTJJOJKDCVZGP-QWRGUYRKSA-N 0.000 description 1
PPGBXYKMUMHFBF-KATARQTJSA-N Leu-Ser-Thr Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(O)=O PPGBXYKMUMHFBF-KATARQTJSA-N 0.000 description 1
YQFZRHYZLARWDY-IHRRRGAJSA-N Leu-Val-Lys Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)N[C@H](C(O)=O)CCCCN YQFZRHYZLARWDY-IHRRRGAJSA-N 0.000 description 1
RKBJFQSKBFWDPQ-UHFFFAOYSA-N Leucinopine Natural products CCCCOC(=O)CNOC(=O)CCCC(=O)O RKBJFQSKBFWDPQ-UHFFFAOYSA-N 0.000 description 1
241000234280 Liliaceae Species 0.000 description 1
241000208204 Linum Species 0.000 description 1
241000209082 Lolium Species 0.000 description 1
235000002262 Lycopersicon Nutrition 0.000 description 1
235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
101710125418 Major capsid protein Proteins 0.000 description 1
241000121629 Majorana Species 0.000 description 1
208000007466 Male Infertility Diseases 0.000 description 1
241000124008 Mammalia Species 0.000 description 1
241001093152 Mangifera Species 0.000 description 1
240000004658 Medicago sativa Species 0.000 description 1
235000010624 Medicago sativa Nutrition 0.000 description 1
OSOLWRWQADPDIQ-DCAQKATOSA-N Met-Asp-Leu Chemical compound [H]N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O OSOLWRWQADPDIQ-DCAQKATOSA-N 0.000 description 1
108090000157 Metallothionein Proteins 0.000 description 1
102000003792 Metallothionein Human genes 0.000 description 1
SIGQQUBJQXSAMW-LURJTMIESA-N Mevalonic acid 5-pyrophosphate Chemical compound OC(=O)C[C@](O)(C)CCOP(O)(=O)OP(O)(O)=O SIGQQUBJQXSAMW-LURJTMIESA-N 0.000 description 1
108020005196 Mitochondrial DNA Proteins 0.000 description 1
229920000881 Modified starch Polymers 0.000 description 1
241000699660 Mus musculus Species 0.000 description 1
101000966481 Mus musculus Dihydrofolate reductase Proteins 0.000 description 1
101000969137 Mus musculus Metallothionein-1 Proteins 0.000 description 1
PYUSHNKNPOHWEZ-YFKPBYRVSA-N N-formyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC=O PYUSHNKNPOHWEZ-YFKPBYRVSA-N 0.000 description 1
125000000729 N-terminal amino-acid group Chemical group 0.000 description 1
101710202365 Napin Proteins 0.000 description 1
235000006508 Nelumbo nucifera Nutrition 0.000 description 1
240000002853 Nelumbo nucifera Species 0.000 description 1
235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
241001282315 Nemesis Species 0.000 description 1
229930193140 Neomycin Natural products 0.000 description 1
241000208125 Nicotiana Species 0.000 description 1
GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
108091092724 Noncoding DNA Proteins 0.000 description 1
101710163270 Nuclease Proteins 0.000 description 1
101710141454 Nucleoprotein Proteins 0.000 description 1
241000795633 Olea <sea slug> Species 0.000 description 1
239000005642 Oleic acid Substances 0.000 description 1
ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
108020005187 Oligonucleotide Probes Proteins 0.000 description 1
102000015636 Oligopeptides Human genes 0.000 description 1
108010038807 Oligopeptides Proteins 0.000 description 1
241000219830 Onobrychis Species 0.000 description 1
241000283973 Oryctolagus cuniculus Species 0.000 description 1
241001147398 Ostrinia nubilalis Species 0.000 description 1
101710093908 Outer capsid protein VP4 Proteins 0.000 description 1
101710135467 Outer capsid protein sigma-1 Proteins 0.000 description 1
108700005081 Overlapping Genes Proteins 0.000 description 1
238000012408 PCR amplification Methods 0.000 description 1
108090000526 Papain Proteins 0.000 description 1
241001494479 Pecora Species 0.000 description 1
241000208181 Pelargonium Species 0.000 description 1
241000209046 Pennisetum Species 0.000 description 1
108010033276 Peptide Fragments Proteins 0.000 description 1
102000007079 Peptide Fragments Human genes 0.000 description 1
241000218196 Persea Species 0.000 description 1
240000007377 Petunia x hybrida Species 0.000 description 1
241000219833 Phaseolus Species 0.000 description 1
235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
244000046052 Phaseolus vulgaris Species 0.000 description 1
102100030368 Phospholipid phosphatase-related protein type 4 Human genes 0.000 description 1
101100523938 Phytophthora infestans (strain T30-4) PexRD21 gene Proteins 0.000 description 1
241000709664 Picornaviridae Species 0.000 description 1
241000219843 Pisum Species 0.000 description 1
235000016816 Pisum sativum subsp sativum Nutrition 0.000 description 1
108010064851 Plant Proteins Proteins 0.000 description 1
206010035226 Plasma cell myeloma Diseases 0.000 description 1
229920000331 Polyhydroxybutyrate Polymers 0.000 description 1
241000710078 Potyvirus Species 0.000 description 1
MDAWMJUZHBQTBO-XGEHTFHBSA-N Pro-Thr-Cys Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CS)C(=O)O)NC(=O)[C@@H]1CCCN1)O MDAWMJUZHBQTBO-XGEHTFHBSA-N 0.000 description 1
KHRLUIPIMIQFGT-AVGNSLFASA-N Pro-Val-Leu Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O KHRLUIPIMIQFGT-AVGNSLFASA-N 0.000 description 1
101710083689 Probable capsid protein Proteins 0.000 description 1
ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
239000004365 Protease Substances 0.000 description 1
101710176177 Protein A56 Proteins 0.000 description 1
102000001253 Protein Kinase Human genes 0.000 description 1
102000009572 RNA Polymerase II Human genes 0.000 description 1
108010009460 RNA Polymerase II Proteins 0.000 description 1
239000013614 RNA sample Substances 0.000 description 1
230000006819 RNA synthesis Effects 0.000 description 1
230000004570 RNA-binding Effects 0.000 description 1
108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
241000218206 Ranunculus Species 0.000 description 1
241000220259 Raphanus Species 0.000 description 1
206010037867 Rash macular Diseases 0.000 description 1
241000700159 Rattus Species 0.000 description 1
101100173636 Rattus norvegicus Fhl2 gene Proteins 0.000 description 1
241000589180 Rhizobium Species 0.000 description 1
241000316848 Rhodococcus <scale insect> Species 0.000 description 1
AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
108091028664 Ribonucleotide Proteins 0.000 description 1
235000011449 Rosa Nutrition 0.000 description 1
241000714474 Rous sarcoma virus Species 0.000 description 1
101100478266 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SPT10 gene Proteins 0.000 description 1
101100397773 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YCK1 gene Proteins 0.000 description 1
241000831652 Salinivibrio sharmensis Species 0.000 description 1
241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
241001106018 Salpiglossis Species 0.000 description 1
101100274510 Schizosaccharomyces pombe (strain 972 / ATCC 24843) cki2 gene Proteins 0.000 description 1
241000780602 Senecio Species 0.000 description 1
108091081021 Sense strand Proteins 0.000 description 1
NLOAIFSWUUFQFR-CIUDSAMLSA-N Ser-Leu-Asp Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(O)=O NLOAIFSWUUFQFR-CIUDSAMLSA-N 0.000 description 1
VMLONWHIORGALA-SRVKXCTJSA-N Ser-Leu-Leu Chemical compound CC(C)C[C@@H](C([O-])=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H]([NH3+])CO VMLONWHIORGALA-SRVKXCTJSA-N 0.000 description 1
241000220261 Sinapis Species 0.000 description 1
235000002634 Solanum Nutrition 0.000 description 1
241000207763 Solanum Species 0.000 description 1
244000062793 Sorghum vulgare Species 0.000 description 1
241000256248 Spodoptera Species 0.000 description 1
241000295644 Staphylococcaceae Species 0.000 description 1
241000187747 Streptomyces Species 0.000 description 1
241000187180 Streptomyces sp. Species 0.000 description 1
235000021536 Sugar beet Nutrition 0.000 description 1
241000701093 Suid alphaherpesvirus 1 Species 0.000 description 1
108010006785 Taq Polymerase Proteins 0.000 description 1
108020005038 Terminator Codon Proteins 0.000 description 1
239000004098 Tetracycline Substances 0.000 description 1
241000219161 Theobroma Species 0.000 description 1
102000002933 Thioredoxin Human genes 0.000 description 1
108010006368 Thioredoxin h Proteins 0.000 description 1
VFEHSAJCWWHDBH-RHYQMDGZSA-N Thr-Arg-Leu Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(O)=O VFEHSAJCWWHDBH-RHYQMDGZSA-N 0.000 description 1
QQWNRERCGGZOKG-WEDXCCLWSA-N Thr-Gly-Leu Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CC(C)C)C(O)=O QQWNRERCGGZOKG-WEDXCCLWSA-N 0.000 description 1
MPUMPERGHHJGRP-WEDXCCLWSA-N Thr-Gly-Lys Chemical compound C[C@H]([C@@H](C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)O)N)O MPUMPERGHHJGRP-WEDXCCLWSA-N 0.000 description 1
RVMNUBQWPVOUKH-HEIBUPTGSA-N Thr-Ser-Thr Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(O)=O RVMNUBQWPVOUKH-HEIBUPTGSA-N 0.000 description 1
108091036066 Three prime untranslated region Proteins 0.000 description 1
108010022394 Threonine synthase Proteins 0.000 description 1
102000006601 Thymidine Kinase Human genes 0.000 description 1
108020004440 Thymidine kinase Proteins 0.000 description 1
102000004357 Transferases Human genes 0.000 description 1
108090000992 Transferases Proteins 0.000 description 1
241000219793 Trifolium Species 0.000 description 1
241001312519 Trigonella Species 0.000 description 1
239000007984 Tris EDTA buffer Substances 0.000 description 1
BSCBBPKDVOZICB-KKUMJFAQSA-N Tyr-Leu-Asp Chemical compound [H]N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(O)=O BSCBBPKDVOZICB-KKUMJFAQSA-N 0.000 description 1
241000700618 Vaccinia virus Species 0.000 description 1
206010046865 Vaccinia virus infection Diseases 0.000 description 1
SZTTYWIUCGSURQ-AUTRQRHGSA-N Val-Glu-Glu Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O SZTTYWIUCGSURQ-AUTRQRHGSA-N 0.000 description 1
241000251539 Vertebrata <Metazoa> Species 0.000 description 1
241000219977 Vigna Species 0.000 description 1
241000219095 Vitis Species 0.000 description 1
235000009392 Vitis Nutrition 0.000 description 1
241000607479 Yersinia pestis Species 0.000 description 1
241000209149 Zea Species 0.000 description 1
101001036768 Zea mays Glucose-1-phosphate adenylyltransferase large subunit 1, chloroplastic/amyloplastic Proteins 0.000 description 1
101000662549 Zea mays Sucrose synthase 1 Proteins 0.000 description 1
HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
230000021736 acetylation Effects 0.000 description 1
238000006640 acetylation reaction Methods 0.000 description 1
108020002494 acetyltransferase Proteins 0.000 description 1
102000005421 acetyltransferase Human genes 0.000 description 1
230000002378 acidificating effect Effects 0.000 description 1
230000003213 activating effect Effects 0.000 description 1
230000004913 activation Effects 0.000 description 1
239000004480 active ingredient Substances 0.000 description 1
239000008186 active pharmaceutical agent Substances 0.000 description 1
230000010933 acylation Effects 0.000 description 1
238000005917 acylation reaction Methods 0.000 description 1
239000000654 additive Substances 0.000 description 1
229960005305 adenosine Drugs 0.000 description 1
238000001042 affinity chromatography Methods 0.000 description 1
239000008272 agar Substances 0.000 description 1
238000000246 agarose gel electrophoresis Methods 0.000 description 1
244000193174 agave Species 0.000 description 1
230000032683 aging Effects 0.000 description 1
230000003281 allosteric effect Effects 0.000 description 1
239000012080 ambient air Substances 0.000 description 1
230000009435 amidation Effects 0.000 description 1
238000007112 amidation reaction Methods 0.000 description 1
150000001408 amides Chemical group 0.000 description 1
229960000723 ampicillin Drugs 0.000 description 1
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
230000001195 anabolic effect Effects 0.000 description 1
210000004102 animal cell Anatomy 0.000 description 1
238000000137 annealing Methods 0.000 description 1
230000000100 anti-cytokinin effect Effects 0.000 description 1
229940088710 antibiotic agent Drugs 0.000 description 1
239000007864 aqueous solution Substances 0.000 description 1
230000010516 arginylation Effects 0.000 description 1
125000003118 aryl group Chemical group 0.000 description 1
229960001230 asparagine Drugs 0.000 description 1
235000009582 asparagine Nutrition 0.000 description 1
108010040443 aspartyl-aspartic acid Proteins 0.000 description 1
210000003719 b-lymphocyte Anatomy 0.000 description 1
238000002869 basic local alignment search tool Methods 0.000 description 1
230000008238 biochemical pathway Effects 0.000 description 1
239000003139 biocide Substances 0.000 description 1
229960001561 bleomycin Drugs 0.000 description 1
OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 1
108010083912 bleomycin N-acetyltransferase Proteins 0.000 description 1
238000007664 blowing Methods 0.000 description 1
230000028446 budding cell bud growth Effects 0.000 description 1
239000007853 buffer solution Substances 0.000 description 1
238000010804 cDNA synthesis Methods 0.000 description 1
239000001506 calcium phosphate Substances 0.000 description 1
229910000389 calcium phosphate Inorganic materials 0.000 description 1
235000011010 calcium phosphates Nutrition 0.000 description 1
239000001390 capsicum minimum Substances 0.000 description 1
125000003917 carbamoyl group Chemical group [H]N([H])C(*)=O 0.000 description 1
230000023852 carbohydrate metabolic process Effects 0.000 description 1
235000021256 carbohydrate metabolism Nutrition 0.000 description 1
239000005018 casein Substances 0.000 description 1
BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
235000021240 caseins Nutrition 0.000 description 1
150000001768 cations Chemical class 0.000 description 1
101150069072 cdc25 gene Proteins 0.000 description 1
230000022131 cell cycle Effects 0.000 description 1
230000010261 cell growth Effects 0.000 description 1
210000002421 cell wall Anatomy 0.000 description 1
239000013043 chemical agent Substances 0.000 description 1
238000007385 chemical modification Methods 0.000 description 1
239000007795 chemical reaction product Substances 0.000 description 1
229960005091 chloramphenicol Drugs 0.000 description 1
WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
239000013611 chromosomal DNA Substances 0.000 description 1
239000013599 cloning vector Substances 0.000 description 1
235000018597 common camellia Nutrition 0.000 description 1
238000004891 communication Methods 0.000 description 1
238000005056 compaction Methods 0.000 description 1
238000012875 competitive assay Methods 0.000 description 1
239000000470 constituent Substances 0.000 description 1
230000002596 correlated effect Effects 0.000 description 1
230000008878 coupling Effects 0.000 description 1
238000010168 coupling process Methods 0.000 description 1
238000005859 coupling reaction Methods 0.000 description 1
244000038559 crop plants Species 0.000 description 1
238000004132 cross linking Methods 0.000 description 1
238000009402 cross-breeding Methods 0.000 description 1
239000011243 crosslinked material Substances 0.000 description 1
239000000287 crude extract Substances 0.000 description 1
238000005520 cutting process Methods 0.000 description 1
230000001351 cycling effect Effects 0.000 description 1
229960003067 cystine Drugs 0.000 description 1
OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical class NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 1
230000000254 damaging effect Effects 0.000 description 1
230000009849 deactivation Effects 0.000 description 1
238000006114 decarboxylation reaction Methods 0.000 description 1
230000002950 deficient Effects 0.000 description 1
230000018044 dehydration Effects 0.000 description 1
238000006297 dehydration reaction Methods 0.000 description 1
230000003111 delayed effect Effects 0.000 description 1
230000017858 demethylation Effects 0.000 description 1
238000010520 demethylation reaction Methods 0.000 description 1
239000005547 deoxyribonucleotide Substances 0.000 description 1
125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
230000000368 destabilizing effect Effects 0.000 description 1
230000004069 differentiation Effects 0.000 description 1
235000019621 digestibility Nutrition 0.000 description 1
102000004419 dihydrofolate reductase Human genes 0.000 description 1
101150052825 dnaK gene Proteins 0.000 description 1
230000002222 downregulating effect Effects 0.000 description 1
238000001035 drying Methods 0.000 description 1
239000012636 effector Substances 0.000 description 1
230000003028 elevating effect Effects 0.000 description 1
239000005712 elicitor Substances 0.000 description 1
230000008030 elimination Effects 0.000 description 1
238000003379 elimination reaction Methods 0.000 description 1
244000088681 endo Species 0.000 description 1
239000002158 endotoxin Substances 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
238000006911 enzymatic reaction Methods 0.000 description 1
238000012869 ethanol precipitation Methods 0.000 description 1
102000013165 exonuclease Human genes 0.000 description 1
238000001400 expression cloning Methods 0.000 description 1
244000037666 field crops Species 0.000 description 1
239000012467 final product Substances 0.000 description 1
238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
230000004907 flux Effects 0.000 description 1
235000013305 food Nutrition 0.000 description 1
230000022244 formylation Effects 0.000 description 1
238000006170 formylation reaction Methods 0.000 description 1
239000012458 free base Substances 0.000 description 1
239000003008 fumonisin Substances 0.000 description 1
108010006664 gamma-glutamyl-glycyl-glycine Proteins 0.000 description 1
238000010363 gene targeting Methods 0.000 description 1
238000010448 genetic screening Methods 0.000 description 1
229960002518 gentamicin Drugs 0.000 description 1
239000008103 glucose Substances 0.000 description 1
229930182478 glucoside Natural products 0.000 description 1
150000008131 glucosides Chemical class 0.000 description 1
125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 description 1
ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
102000005396 glutamine synthetase Human genes 0.000 description 1
108020002326 glutamine synthetase Proteins 0.000 description 1
230000001279 glycosylating effect Effects 0.000 description 1
108010089804 glycyl-threonine Proteins 0.000 description 1
239000011544 gradient gel Substances 0.000 description 1
239000003102 growth factor Substances 0.000 description 1
229940029575 guanosine Drugs 0.000 description 1
150000003278 haem Chemical group 0.000 description 1
230000009931 harmful effect Effects 0.000 description 1
238000003306 harvesting Methods 0.000 description 1
230000036541 health Effects 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
230000007062 hydrolysis Effects 0.000 description 1
238000006460 hydrolysis reaction Methods 0.000 description 1
238000003365 immunocytochemistry Methods 0.000 description 1
230000005847 immunogenicity Effects 0.000 description 1
238000007901 in situ hybridization Methods 0.000 description 1
238000010249 in-situ analysis Methods 0.000 description 1
238000009399 inbreeding Methods 0.000 description 1
238000011534 incubation Methods 0.000 description 1
239000003617 indole-3-acetic acid Substances 0.000 description 1
239000000411 inducer Substances 0.000 description 1
206010022000 influenza Diseases 0.000 description 1
238000011081 inoculation Methods 0.000 description 1
229960003786 inosine Drugs 0.000 description 1
230000016507 interphase Effects 0.000 description 1
238000011835 investigation Methods 0.000 description 1
230000026045 iodination Effects 0.000 description 1
150000002500 ions Chemical class 0.000 description 1
238000006317 isomerization reaction Methods 0.000 description 1
QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
238000005304 joining Methods 0.000 description 1
238000011005 laboratory method Methods 0.000 description 1
108010000761 leucylarginine Proteins 0.000 description 1
108010057821 leucylproline Proteins 0.000 description 1
108010012058 leucyltyrosine Proteins 0.000 description 1
230000000670 limiting effect Effects 0.000 description 1
239000002502 liposome Substances 0.000 description 1
210000004698 lymphocyte Anatomy 0.000 description 1
125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
230000002934 lysing effect Effects 0.000 description 1
230000014759 maintenance of location Effects 0.000 description 1
210000004962 mammalian cell Anatomy 0.000 description 1
235000005739 manihot Nutrition 0.000 description 1
201000001441 melanoma Diseases 0.000 description 1
QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
229910052753 mercury Inorganic materials 0.000 description 1
230000004060 metabolic process Effects 0.000 description 1
MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 1
229960000485 methotrexate Drugs 0.000 description 1
FUZOUVDGBZTBCF-UHFFFAOYSA-N methyl 7-[2-[2-(8-methoxycarbonyl-4b,8-dimethyl-5,6,7,8a,9,10-hexahydrophenanthren-2-yl)propan-2-ylperoxy]propan-2-yl]-1,4a-dimethyl-2,3,4,9,10,10a-hexahydrophenanthrene-1-carboxylate Chemical compound C1=C2CCC3C(C)(C(=O)OC)CCCC3(C)C2=CC=C1C(C)(C)OOC(C)(C)C1=CC=C2C3(C)CCCC(C(=O)OC)(C)C3CCC2=C1 FUZOUVDGBZTBCF-UHFFFAOYSA-N 0.000 description 1
230000011987 methylation Effects 0.000 description 1
238000007069 methylation reaction Methods 0.000 description 1
230000000813 microbial effect Effects 0.000 description 1
244000000010 microbial pathogen Species 0.000 description 1
235000019426 modified starch Nutrition 0.000 description 1
230000000921 morphogenic effect Effects 0.000 description 1
230000004660 morphological change Effects 0.000 description 1
239000006870 ms-medium Substances 0.000 description 1
201000000050 myeloid neoplasm Diseases 0.000 description 1
230000007498 myristoylation Effects 0.000 description 1
229930014626 natural product Natural products 0.000 description 1
239000006225 natural substrate Substances 0.000 description 1
229960004927 neomycin Drugs 0.000 description 1
229910017604 nitric acid Inorganic materials 0.000 description 1
230000031787 nutrient reservoir activity Effects 0.000 description 1
ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
229940046166 oligodeoxynucleotide Drugs 0.000 description 1
239000002751 oligonucleotide probe Substances 0.000 description 1
238000011275 oncology therapy Methods 0.000 description 1
238000005457 optimization Methods 0.000 description 1
230000005305 organ development Effects 0.000 description 1
210000003463 organelle Anatomy 0.000 description 1
238000009401 outcrossing Methods 0.000 description 1
239000003973 paint Substances 0.000 description 1
238000004091 panning Methods 0.000 description 1
229940055729 papain Drugs 0.000 description 1
235000019834 papain Nutrition 0.000 description 1
230000036961 partial effect Effects 0.000 description 1
229940083251 peripheral vasodilators purine derivative Drugs 0.000 description 1
210000001322 periplasm Anatomy 0.000 description 1
238000002823 phage display Methods 0.000 description 1
150000003905 phosphatidylinositols Chemical class 0.000 description 1
150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
230000026731 phosphorylation Effects 0.000 description 1
238000006366 phosphorylation reaction Methods 0.000 description 1
230000029553 photosynthesis Effects 0.000 description 1
238000010672 photosynthesis Methods 0.000 description 1
230000000243 photosynthetic effect Effects 0.000 description 1
230000010254 physiological adaptation Effects 0.000 description 1
230000001766 physiological effect Effects 0.000 description 1
230000035790 physiological processes and functions Effects 0.000 description 1
230000019612 pigmentation Effects 0.000 description 1
239000003375 plant hormone Substances 0.000 description 1
238000004161 plant tissue culture Methods 0.000 description 1
235000021118 plant-derived protein Nutrition 0.000 description 1
230000004983 pleiotropic effect Effects 0.000 description 1
230000007198 pollen germination Effects 0.000 description 1
239000005015 poly(hydroxybutyrate) Substances 0.000 description 1
229920003223 poly(pyromellitimide-1,4-diphenyl ether) Polymers 0.000 description 1
230000008488 polyadenylation Effects 0.000 description 1
238000011176 pooling Methods 0.000 description 1
230000023603 positive regulation of transcription initiation, DNA-dependent Effects 0.000 description 1
230000001323 posttranslational effect Effects 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
230000013823 prenylation Effects 0.000 description 1
230000001737 promoting effect Effects 0.000 description 1
108060006633 protein kinase Proteins 0.000 description 1
230000009145 protein modification Effects 0.000 description 1
238000001243 protein synthesis Methods 0.000 description 1
150000003212 purines Chemical class 0.000 description 1
NHDHVHZZCFYRSB-UHFFFAOYSA-N pyriproxyfen Chemical compound C=1C=CC=NC=1OC(C)COC(C=C1)=CC=C1OC1=CC=CC=C1 NHDHVHZZCFYRSB-UHFFFAOYSA-N 0.000 description 1
229940043131 pyroglutamate Drugs 0.000 description 1
230000006340 racemization Effects 0.000 description 1
230000002285 radioactive effect Effects 0.000 description 1
238000003127 radioimmunoassay Methods 0.000 description 1
238000009790 rate-determining step (RDS) Methods 0.000 description 1
239000011541 reaction mixture Substances 0.000 description 1
238000010188 recombinant method Methods 0.000 description 1
238000011084 recovery Methods 0.000 description 1
230000022983 regulation of cell cycle Effects 0.000 description 1
230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
108091008025 regulatory factors Proteins 0.000 description 1
102000037983 regulatory factors Human genes 0.000 description 1
230000008439 repair process Effects 0.000 description 1
108091008020 response regulators Proteins 0.000 description 1
230000003938 response to stress Effects 0.000 description 1
230000001177 retroviral effect Effects 0.000 description 1
230000002441 reversible effect Effects 0.000 description 1
239000002336 ribonucleotide Substances 0.000 description 1
125000002652 ribonucleotide group Chemical group 0.000 description 1
150000008223 ribosides Chemical class 0.000 description 1
238000007363 ring formation reaction Methods 0.000 description 1
230000002786 root growth Effects 0.000 description 1
238000005070 sampling Methods 0.000 description 1
229920006395 saturated elastomer Polymers 0.000 description 1
150000004671 saturated fatty acids Chemical class 0.000 description 1
235000003441 saturated fatty acids Nutrition 0.000 description 1
230000014284 seed dormancy process Effects 0.000 description 1
230000035040 seed growth Effects 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(I) nitrate Inorganic materials [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 1
239000001509 sodium citrate Substances 0.000 description 1
SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
210000001082 somatic cell Anatomy 0.000 description 1
230000030118 somatic embryogenesis Effects 0.000 description 1
108010048090 soybean lectin Proteins 0.000 description 1
230000000087 stabilizing effect Effects 0.000 description 1
229910001220 stainless steel Inorganic materials 0.000 description 1
239000010935 stainless steel Substances 0.000 description 1
229960005322 streptomycin Drugs 0.000 description 1
108010008664 streptomycin 3''-kinase Proteins 0.000 description 1
229940124530 sulfonamide Drugs 0.000 description 1
150000003456 sulfonamides Chemical class 0.000 description 1
235000020238 sunflower seed Nutrition 0.000 description 1
230000002194 synthesizing effect Effects 0.000 description 1
238000010189 synthetic method Methods 0.000 description 1
229920002475 tRNA polymer Polymers 0.000 description 1
229960002180 tetracycline Drugs 0.000 description 1
229930101283 tetracycline Natural products 0.000 description 1
235000019364 tetracycline Nutrition 0.000 description 1
150000003522 tetracyclines Chemical class 0.000 description 1
239000004753 textile Substances 0.000 description 1
108060008226 thioredoxin Proteins 0.000 description 1
108010061238 threonyl-glycine Proteins 0.000 description 1
238000012090 tissue culture technique Methods 0.000 description 1
231100000331 toxic Toxicity 0.000 description 1
231100000167 toxic agent Toxicity 0.000 description 1
230000002588 toxic effect Effects 0.000 description 1
239000003440 toxic substance Substances 0.000 description 1
238000011426 transformation method Methods 0.000 description 1
238000011830 transgenic mouse model Methods 0.000 description 1
230000007704 transition Effects 0.000 description 1
230000005068 transpiration Effects 0.000 description 1
230000032258 transport Effects 0.000 description 1
QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
229940038773 trisodium citrate Drugs 0.000 description 1
241001430294 unidentified retrovirus Species 0.000 description 1
150000004670 unsaturated fatty acids Chemical class 0.000 description 1
235000021122 unsaturated fatty acids Nutrition 0.000 description 1
208000007089 vaccinia Diseases 0.000 description 1
210000005167 vascular cell Anatomy 0.000 description 1
210000003462 vein Anatomy 0.000 description 1
230000001018 virulence Effects 0.000 description 1
230000000007 visual effect Effects 0.000 description 1
238000003260 vortexing Methods 0.000 description 1
238000005406 washing Methods 0.000 description 1
230000003442 weekly effect Effects 0.000 description 1
238000001262 western blot Methods 0.000 description 1
229910052725 zinc Inorganic materials 0.000 description 1
239000011701 zinc Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
- C12N9/0032—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with oxygen as acceptor (1.5.3)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/823—Reproductive tissue-specific promoters
- C12N15/8234—Seed-specific, e.g. embryo, endosperm
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Zoology (AREA)
Organic Chemistry (AREA)
Biomedical Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Cell Biology (AREA)
Physics & Mathematics (AREA)
Medicinal Chemistry (AREA)
Plant Pathology (AREA)
Developmental Biology & Embryology (AREA)
Pregnancy & Childbirth (AREA)
Reproductive Health (AREA)
Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

CA2521497A 2003-04-04 2004-04-02 Modulation de l'activite des cytokinines dans les plantes Expired - Fee Related CA2521497C (fr)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US46071803P	2003-04-04	2003-04-04
US60/460,718		2003-04-04
PCT/US2004/010064 WO2004090143A2 (fr)	2003-04-04	2004-04-02	Modulation de l'activite des cytokinines dans les plantes

Publications (2)

Publication Number	Publication Date
CA2521497A1 CA2521497A1 (fr)	2004-10-21
CA2521497C true CA2521497C (fr)	2012-11-27

Family

ID=33159802

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA2521497A Expired - Fee Related CA2521497C (fr)	2003-04-04	2004-04-02	Modulation de l'activite des cytokinines dans les plantes

Country Status (9)

Country	Link
EP (1)	EP1613754A2 (fr)
CN (3)	CN1798843A (fr)
AR (1)	AR045420A1 (fr)
AU (1)	AU2004227360B2 (fr)
BR (1)	BRPI0409178A (fr)
CA (1)	CA2521497C (fr)
NZ (1)	NZ543070A (fr)
WO (1)	WO2004090143A2 (fr)
ZA (1)	ZA200508006B (fr)

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US7371925B2 (en) *	2001-03-29	2008-05-13	Pioneer Hi-Bred International, Inc.	Cytokinin oxidase promoter from maize
CA2485689A1 (fr) *	2002-06-19	2003-12-31	Basf Plant Science Gmbh	Compositions et procedes pour ameliorer les performances des plantes
EP1741788A1 (fr) *	2005-07-06	2007-01-10	Freie Universität Berlin	Plante transgénique ayant une activité des récepteurs aux cytokinines réduite de manière sélective dans certains tissus
US20080301839A1 (en) *	2005-08-30	2008-12-04	Ravanello Monica P	Transgenic plants with enhanced agronomic traits
CA2743707A1 (fr)	2008-12-04	2010-06-10	Pioneer Hi-Bred International, Inc.	Procedes et compositions pour un rendement ameliore par une expression ciblee de knotted1
CA2765034A1 (fr)	2009-06-09	2010-12-23	Pioneer Hi-Bred International, Inc.	Promoteur d'endosperme precoce et procedes d'utilisation
US8440891B2 (en)	2009-09-22	2013-05-14	Board of Trustees of the University of Akransas, N.A.	Rice cultivar CL 142-AR
US8440892B2 (en)	2009-10-15	2013-05-14	Board Of Trustees Of The University Of Arkansas, N.A.	Rice cultivar CL 181-AR
US8778672B2 (en)	2009-10-26	2014-07-15	Pioneer Hi Bred International Inc	Somatic ovule specific promoter and methods of use
EP2512226B1 (fr) *	2009-12-17	2019-05-01	Pioneer Hi-Bred International, Inc.	Evénement de transformation dp-004114-3 du maïs et son procédé de détection
CN101899442B (zh) *	2010-07-13	2012-02-01	中国科学院遗传与发育生物学研究所	水稻rr17启动子及其应用
US20130167262A1 (en)	2011-12-21	2013-06-27	The Curators Of The University Of Missouri	Soybean variety s05-11268
WO2013096810A1 (fr)	2011-12-21	2013-06-27	The Curators Of The University Of Missouri	Variété de soja s05-11482
BR112014016791A2 (pt)	2012-01-06	2019-09-24	Pioneer Hi Bred Int	molécula de ácido nucléico isolada, cassete de expressão, vetor, célula vegetal, planta, semente transgénica, método para expressão de um polinucleotídeo em uma planta ou célula vegetal, método para expressão de um polinucleotídeo, preferencialmente em tecidos de óvulo de uma planta
US9006515B2 (en)	2012-01-06	2015-04-14	Pioneer Hi Bred International Inc	Pollen preferred promoters and methods of use
MX2014011044A (es) *	2012-03-13	2015-05-15	Pioneer Hi Bred Int	Reduccion genetica de la fertilidad masculina en plantas.
CN102629304B (zh) *	2012-04-05	2015-02-04	天津大学	基于基因组尺度代谢网络模型的代谢工程设计预测方法
US20150259696A1 (en)	2012-10-11	2015-09-17	Shane E. Abbitt	Guard cell promoters and uses thereof
WO2014159306A1 (fr)	2013-03-13	2014-10-02	Pioneer Hi-Bred International, Inc.	Application de glyphosate pour désherbage d'une brassicée
BR112015023285B1 (pt)	2013-03-14	2022-03-03	Pioneer Hi-Bred International, Inc.	Cassete de expressão, célula hospedeira bacteriana e método para controlar uma praga de planta do tipo coleóptero
CA2901316A1 (fr)	2013-03-15	2014-09-25	Pioneer Hi-Bred International, Inc.	Polypeptides phi-4 et leurs procedes d'utilisation
MX359026B (es)	2013-08-16	2018-09-12	Pioneer Hi Bred Int	Proteinas insecticidas y metodos de uso.
CA3223359A1 (fr)	2013-09-13	2015-03-19	Pioneer Hi-Bred International, Inc.	Proteines insecticides et leurs procedes d'utilisation
CA3290904A1 (en)	2014-02-07	2026-03-02	E. I. Du Pont De Nemours And Company	Insecticidal proteins and methods for their use
CA2955828A1 (fr)	2014-08-08	2016-02-11	Pioneer Hi-Bred International, Inc.	Promoteurs d'ubiquitine et introns, et procedes d'utilisation
CA2961733A1 (fr)	2014-09-17	2016-03-24	Pioneer Hi-Bred International, Inc.	Compositions et procedes de lutte contre des insectes ravageurs
CA2963558C (fr)	2014-10-16	2023-04-04	Pioneer Hi-Bred International, Inc.	Proteines insecticides et leurs procedes d'utilisation
US20170359965A1 (en)	2014-12-19	2017-12-21	E I Du Pont De Nemours And Company	Polylactic acid compositions with accelerated degradation rate and increased heat stability
CN107635396B (zh)	2015-01-15	2021-12-24	先锋国际良种公司	杀昆虫蛋白及其使用方法
CN116333064A (zh)	2015-05-19	2023-06-27	先锋国际良种公司	杀昆虫蛋白及其使用方法
AU2016278142A1 (en)	2015-06-16	2017-11-30	E. I. Du Pont De Nemours And Company	Compositions and methods to control insect pests
CA3268091A1 (en)	2015-08-06	2026-03-02	Pioneer Hi-Bred International, Inc.	Plant derived insecticidal proteins and methods for their use
CN108513584A (zh)	2015-08-28	2018-09-07	先锋国际良种公司	苍白杆菌介导的植物转化
WO2017105987A1 (fr)	2015-12-18	2017-06-22	Pioneer Hi-Bred International, Inc.	Protéines insecticides et leurs procédés d'utilisation
EP3394268B1 (fr)	2015-12-22	2023-07-19	Pioneer Hi-Bred International, Inc.	Promoteurs préférés par un tissu et procédés d'utilisation
CN105624190A (zh) *	2016-03-23	2016-06-01	东北农业大学	一种转异戊烯基酰基转移酶基因玉米育种方法
MX387077B (es)	2016-05-04	2025-03-19	Pioneer Hi Bred Int	Proteinas insecticidas y metodos para sus usos.
WO2017218207A1 (fr)	2016-06-16	2017-12-21	Pioneer Hi-Bred International, Inc.	Compositions et procédés de lutte contre des insectes nuisibles
ES2924552T3 (es)	2016-06-24	2022-10-07	Pioneer Hi Bred Int	Elementos reguladores de las plantas y métodos de uso de los mismos
CA3026113A1 (fr)	2016-07-01	2018-01-04	Pioneer Hi-Bred International, Inc.	Proteines insecticides issues de plantes et procedes pour leur utilisation
WO2018013333A1 (fr)	2016-07-12	2018-01-18	Pioneer Hi-Bred International, Inc.	Compositions et procédés de lutte contre des insectes nuisibles
MX387927B (es)	2016-11-01	2025-03-19	Pioneer Hi Bred Int	Proteinas insecticidas y metodos para su uso.
JP2020536515A (ja)	2017-09-25	2020-12-17	パイオニアハイ−ブレッドインターナショナル，インコーポレイテッド	組織優先的プロモーター及びその使用方法
EP4549576A3 (fr)	2018-03-12	2025-07-30	Pioneer Hi-Bred International, Inc.	Procédés de transformation de plantes
CN111867377B (zh)	2018-03-14	2023-05-23	先锋国际良种公司	来自植物的杀昆虫蛋白及其使用方法
AU2019234566B2 (en)	2018-03-14	2024-09-26	Hexima Limited	Insecticidal proteins from plants and methods for their use
US11702668B2 (en)	2018-05-22	2023-07-18	Pioneer Hi-Bred International, Inc.	Plant regulatory elements and methods of use thereof
US20210277409A1 (en)	2018-06-28	2021-09-09	Pioneer Hi-Bred International, Inc.	Methods for selecting transformed plants
US20210395758A1 (en)	2018-10-31	2021-12-23	Pioneer Hi-Bred International, Inc.	Compositions and methods for ochrobactrum-mediated plant transformation
CN109762837B (zh) *	2018-12-24	2020-12-15	中国医学科学院药用植物研究所	利用丹参长链异戊烯基转移酶基因SmPPS2调控辅酶Q合成的方法
CN109735538B (zh) *	2018-12-27	2022-06-21	南京农业大学	一种提高森林草莓叶片再生效率的载体及其制备方法和应用
CN111518816B (zh) *	2020-05-12	2021-10-08	广西大学	一种玉米黑暗响应白化/玻璃黄色12基因及其应用
US12168774B2 (en)	2020-07-14	2024-12-17	Pioneer Hi-Bred International, Inc.	Insecticidal proteins and methods for their use
CN114990112B (zh) *	2021-02-26	2024-03-12	华中农业大学	一种皮刺特异启动子
CN114807181A (zh) *	2022-04-30	2022-07-29	浙江师范大学	水稻OsCKX3基因在调控水稻叶夹角中的应用
CN118975473B (zh) *	2024-08-05	2025-05-27	山东滨州国家农业科技园区管理服务中心(滨州黄河三角洲高效生态产业现代技术研究院)	一种北方盐碱地引种大青枣的花期调控方法
EP4725303A1 (fr)	2024-10-11	2026-04-15	Agrokray Limited Liability Company	Procede de production de plantes sphellantes, grain produit a partir de plantes spellantes et produit alimentaire produit a partir de celles-ci

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4945050A (en)	1984-11-13	1990-07-31	Cornell Research Foundation, Inc.	Method for transporting substances into living cells and tissues and apparatus therefor
ATE57390T1 (de)	1986-03-11	1990-10-15	Plant Genetic Systems Nv	Durch gentechnologie erhaltene und gegen glutaminsynthetase-inhibitoren resistente pflanzenzellen.
US5453566A (en)	1986-03-28	1995-09-26	Calgene, Inc.	Antisense regulation of gene expression in plant/cells
US6329570B1 (en) *	1989-07-19	2001-12-11	Calgene, Llc	Cotton modification using ovary-tissue transcriptional factors
PL295547A1 (fr)	1990-04-26	1992-10-05	Plant Genetic Systems Nv
US6329520B1 (en) *	1990-08-09	2001-12-11	Research Triangle Institute	Cocaine receptor binding ligands
US5277905A (en)	1991-01-16	1994-01-11	Mycogen Corporation	Coleopteran-active bacillus thuringiensis isolate
DE69227911T2 (de)	1991-08-02	1999-05-12	Kubota Corp., Tokio/Tokyo	Neuer mikroorganismus und insektizid
GB9210273D0 (en)	1992-05-13	1992-07-01	Ici Plc	Dna
EP0625006A4 (en)	1992-11-20	1996-04-24	Agracetus	Transgenic cotton plants producing heterologous bioplastic.
US5593881A (en)	1994-05-06	1997-01-14	Mycogen Corporation	Bacillus thuringiensis delta-endotoxin
US5736369A (en)	1994-07-29	1998-04-07	Pioneer Hi-Bred International, Inc.	Method for producing transgenic cereal plants
US5792931A (en)	1994-08-12	1998-08-11	Pioneer Hi-Bred International, Inc.	Fumonisin detoxification compositions and methods
US5689042A (en) *	1995-03-29	1997-11-18	Wisconsin Alumni Research Foundation	Transgenic plants with altered senescence characteristics
DE69633568T2 (de) *	1995-07-26	2005-12-22	Pioneer Hi-Bred International, Inc.	Expressionskontrollesequenz zur allgemeinen und effektiven genexpression in pflanzen
US5737514A (en)	1995-11-29	1998-04-07	Texas Micro, Inc.	Remote checkpoint memory system and protocol for fault-tolerant computer system
AU5489099A (en) *	1998-08-20	2000-03-14	Pioneer Hi-Bred International, Inc.	Seed-preferred promoters
MXPA01010439A (es) *	1999-04-16	2003-09-10	Pioneer Hi Bred Int	Expresion regulada de genes en semillas.
US7531723B2 (en)	1999-04-16	2009-05-12	Pioneer Hi-Bred International, Inc.	Modulation of cytokinin activity in plants
US6407315B1 (en) *	1999-11-02	2002-06-18	Pioneer Hi-Bred International, Inc.	Seed-preferred promoter from barley
WO2002078438A2 (fr) *	2001-03-29	2002-10-10	Pioneer Hi-Bred International, Inc.	Promoteur du mais prefere des tissus

2004
- 2004-04-02 CA CA2521497A patent/CA2521497C/fr not_active Expired - Fee Related
- 2004-04-02 BR BRPI0409178-7A patent/BRPI0409178A/pt not_active Application Discontinuation
- 2004-04-02 CN CNA2004800155350A patent/CN1798843A/zh active Pending
- 2004-04-02 CN CNA2008100089927A patent/CN101220357A/zh active Pending
- 2004-04-02 AU AU2004227360A patent/AU2004227360B2/en not_active Ceased
- 2004-04-02 EP EP04749636A patent/EP1613754A2/fr not_active Withdrawn
- 2004-04-02 WO PCT/US2004/010064 patent/WO2004090143A2/fr not_active Ceased
- 2004-04-02 CN CNA2008100089946A patent/CN101220358A/zh active Pending
- 2004-04-02 NZ NZ543070A patent/NZ543070A/en not_active IP Right Cessation
- 2004-04-06 AR ARP040101154A patent/AR045420A1/es active IP Right Grant
2005
- 2005-10-04 ZA ZA200508006A patent/ZA200508006B/en unknown

Also Published As

Publication number	Publication date
WO2004090143A2 (fr)	2004-10-21
CN1798843A (zh)	2006-07-05
CN101220358A (zh)	2008-07-16
BRPI0409178A (pt)	2006-05-02
CA2521497A1 (fr)	2004-10-21
CN101220357A (zh)	2008-07-16
ZA200508006B (en)	2007-01-31
NZ543070A (en)	2008-10-31
AU2004227360A1 (en)	2004-10-21
AU2004227360B2 (en)	2009-05-28
WO2004090143A3 (fr)	2005-05-12
EP1613754A2 (fr)	2006-01-11
AR045420A1 (es)	2005-10-26

Legal Events

Date	Code	Title	Description
2005-10-05	EEER	Examination request
2016-05-16	MKLA	Lapsed	Effective date: 20160404

Publication	Publication Date	Title
CA2521497C (fr)	2012-11-27	Modulation de l'activite des cytokinines dans les plantes
US7531723B2 (en)	2009-05-12	Modulation of cytokinin activity in plants
US6992237B1 (en)	2006-01-31	Regulated expression of genes in plant seeds
CA2370594C (fr)	2007-01-09	Expression regulee de genes dans des graines de plante
US6225529B1 (en)	2001-05-01	Seed-preferred promoters
US7432418B2 (en)	2008-10-07	Seed-preferred regulatory elements and uses thereof
US20020148007A1 (en)	2002-10-10	Seed-preferred promoter from barley
US20130133110A1 (en)	2013-05-23	Transcriptional activators involved in abiotic stress tolerance
US20020152500A1 (en)	2002-10-17	Tissue-preferred promoter from maize
US7253000B2 (en)	2007-08-07	Transcriptional activators involved in abiotic stress tolerance
US7321031B2 (en)	2008-01-22	Seed preferred regulatory elements
US20040210043A1 (en)	2004-10-21	Seed preferred regulatory elements
US20040139506A1 (en)	2004-07-15	Auxin-repressed, dormancy-associated promoter and uses thereof
MXPA05010675A (en)	2006-10-17	Modulation of cytokinin activity in plants
ZA200601187B (en)	2007-05-30	Materials and methods for the modulation of cyclin-dependent kinase inhibitor-like polypeptides in maize