CA2719863C - Detection et denombrement de microorganismes - Google Patents

Detection et denombrement de microorganismes Download PDF

Info

Publication number
CA2719863C
CA2719863C CA2719863A CA2719863A CA2719863C CA 2719863 C CA2719863 C CA 2719863C CA 2719863 A CA2719863 A CA 2719863A CA 2719863 A CA2719863 A CA 2719863A CA 2719863 C CA2719863 C CA 2719863C
Authority
CA
Canada
Prior art keywords
probe
microorganisms
sample
nucleic acids
legionella pneumophila
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2719863A
Other languages
English (en)
Other versions
CA2719863A1 (fr
Inventor
Yannick Fovet
Sam Dukan
Adrien Ducret
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF SE
Centre National de la Recherche Scientifique CNRS
Original Assignee
BASF SE
Centre National de la Recherche Scientifique CNRS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB0806136.8A external-priority patent/GB0806136D0/en
Priority claimed from GB0900848A external-priority patent/GB0900848D0/en
Application filed by BASF SE, Centre National de la Recherche Scientifique CNRS filed Critical BASF SE
Publication of CA2719863A1 publication Critical patent/CA2719863A1/fr
Application granted granted Critical
Publication of CA2719863C publication Critical patent/CA2719863C/fr
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2719863A 2008-04-04 2009-03-20 Detection et denombrement de microorganismes Expired - Fee Related CA2719863C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB0806136.8 2008-04-04
GBGB0806136.8A GB0806136D0 (en) 2008-04-04 2008-04-04 Detection and enumeration of microorganisms
GB0900848.3 2009-01-20
GB0900848A GB0900848D0 (en) 2009-01-20 2009-01-20 Detection and enumeration of microorganisms
PCT/EP2009/053299 WO2009121727A1 (fr) 2008-04-04 2009-03-20 Détection et dénombrement de microorganismes

Publications (2)

Publication Number Publication Date
CA2719863A1 CA2719863A1 (fr) 2009-10-08
CA2719863C true CA2719863C (fr) 2013-10-08

Family

ID=40887000

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2719863A Expired - Fee Related CA2719863C (fr) 2008-04-04 2009-03-20 Detection et denombrement de microorganismes

Country Status (18)

Country Link
US (1) US20110076686A1 (fr)
EP (1) EP2262910B1 (fr)
JP (1) JP5349579B2 (fr)
KR (1) KR101265412B1 (fr)
CN (1) CN101990579A (fr)
AU (1) AU2009231362B2 (fr)
CA (1) CA2719863C (fr)
CY (1) CY1114785T1 (fr)
DK (1) DK2262910T3 (fr)
ES (1) ES2389861T3 (fr)
HR (1) HRP20120553T1 (fr)
IL (1) IL208158A0 (fr)
MX (1) MX2010010385A (fr)
MY (1) MY149655A (fr)
PT (1) PT2262910E (fr)
RS (1) RS52445B (fr)
RU (1) RU2477319C2 (fr)
WO (1) WO2009121727A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5717846B2 (ja) * 2010-06-03 2015-05-13 ビーエーエスエフ ソシエタス・ヨーロピアＢａｓｆ Ｓｅ 微生物の検出及び定量化
CN105524974A (zh) * 2016-02-24 2016-04-27 北京世纪阿姆斯生物技术有限公司 一种高浓度微生物制剂产品含菌量的检测方法
CN107656056B (zh) * 2017-08-29 2019-05-28 山东师范大学 一种基于细菌增长对细菌快速镜检的方法
RU2699983C1 (ru) * 2018-12-04 2019-09-11 Федеральное бюджетное учреждение науки "Государственный научный центр прикладной микробиологии и биотехнологии" (ФБУН ГНЦ ПМБ) Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека Штамм гибридных культивируемых клеток животных Mus musculus 1F11PAL - продуцент мышиных моноклональных антител, специфичных к пептидогликан-ассоциированному липопротеину (PAL) Legionella pneumophila
EP3904530A1 (fr) * 2020-04-27 2021-11-03 Universidade de Évora Procédé de collecte, de détection et/ou d'identification de micro-organismes à partir d'une pierre naturelle et ses utilisations
CN116879548A (zh) * 2023-05-08 2023-10-13 中国计量科学研究院 一种原奶中活菌总数的特异性快速定量检测方法
CN119331943A (zh) * 2024-11-01 2025-01-21 郑州安图生物工程股份有限公司 一种军团菌培养检测试剂盒及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5834445A (en) * 1989-08-11 1998-11-10 Sikorski; Christopher Process for preparing decolorized carotenoid-cyclodextrin complexes
FR2845097B1 (fr) * 2002-10-01 2006-06-16 Metis Biotechnologies Procede de detection et de comptage de microorganismes dans un echantillon
JP4632839B2 (ja) * 2005-03-31 2011-02-16 アクアス株式会社 レジオネラ検査用選択培地、及び、レジオネラ属菌の培養方法
US20090239256A1 (en) * 2005-06-09 2009-09-24 South Australian Water Corporation Detection of Micro-Organisms
ATE531818T1 (de) * 2006-05-02 2011-11-15 Univ Paris Curie Methode zur bestimmung und auszählung von mikroorganismen

Also Published As

Publication number Publication date
AU2009231362B2 (en) 2012-07-05
KR101265412B1 (ko) 2013-05-16
CN101990579A (zh) 2011-03-23
RS52445B (sr) 2013-02-28
RU2010144839A (ru) 2012-05-20
CY1114785T1 (el) 2016-12-14
ES2389861T3 (es) 2012-11-02
MX2010010385A (es) 2010-12-15
US20110076686A1 (en) 2011-03-31
EP2262910B1 (fr) 2012-06-06
IL208158A0 (en) 2010-12-30
JP2011516052A (ja) 2011-05-26
AU2009231362A1 (en) 2009-10-08
KR20100134093A (ko) 2010-12-22
JP5349579B2 (ja) 2013-11-20
CA2719863A1 (fr) 2009-10-08
WO2009121727A1 (fr) 2009-10-08
PT2262910E (pt) 2012-07-18
RU2477319C2 (ru) 2013-03-10
MY149655A (en) 2013-09-30
DK2262910T3 (da) 2012-09-17
HRP20120553T1 (hr) 2012-10-31
EP2262910A1 (fr) 2010-12-22

Similar Documents

Publication Publication Date Title
CA2719863C (fr) Detection et denombrement de microorganismes
AU651077B2 (en) Process for detection of water-borne microbial pathogens and indicators of human fecal contamination in water samples and kits therefor
AU2003283484B2 (en) Method for detecting and counting micro-organisms in a sample
US20160076085A1 (en) Methods for detecting and quantifying viable bacterial endo-spores
Baymiev et al. Modern approaches to differentiation of live and dead bacteria using selective amplification of nucleic acids
BRPI0710889A2 (pt) método para a detecção e quantificação múltipla e simultánea de patogênicos mediante a reação em cadeia da polimerasa em tempo real
JP3934605B2 (ja) 感染症原因微生物の検出および同定のための改良された方法
EP2262907B1 (fr) Détection et dénombrement de micro-organismes
CA2369577A1 (fr) Procede de detection de micro-organismes dans un echantillon
US20090136930A1 (en) Method for the identification of microorganisms by means of in situ hybridization and flow cytometry
EP2016192A2 (fr) Méthode pour la détection et l'énumération de microorganismes
US6905824B2 (en) Methods for determining organisms not requiring the separation of fixative or excess probe
AU2003226729A1 (en) Method for the identification of microorganisms by means of in situ hybridization and flow cytometry
JP4104377B2 (ja) シュードモナス・アエルギノーザ菌の検出用プローブおよびそれを用いた方法
EP2553120B1 (fr) Sonde d'acide nucléique peptidique, trousse et procédé pour la détection et/ou la quantification de salmonella spp. et applications correspondantes
AU707811B2 (en) Method for the detection of viable Cryptosporidium parvum oocysts
JP2025043943A (ja) 細菌からの核酸抽出法
JP2004147550A (ja) 核酸断片、微生物の検出方法及び微生物検出用キット

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20160321