CN106922706A - a bactericidal composition - Google Patents

Abstract

The present invention provides a fungicide composition comprising the active ingredients Picarbutrazox and trifloxystrobin or picoxystrobin, wherein the weight ratio of Picarbutrazox to trifloxystrobin or picoxystrobin is 50:1 to 1:50. By combining Picarbutrazox and trifloxystrobin or picoxystrobin in a binary combination, the present invention achieves a significant benefit in preventing and controlling fungal and bacterial diseases on crops. Furthermore, the binary combination expands the fungicide spectrum, reduces the dosage of each agent, and reduces the risk of pathogenic bacteria developing drug resistance.

Description

a bactericidal composition

technical field

The present invention relates to a fungicidal composition, in particular to a fungicidal composition for protecting plants, crops or seeds from fungal diseases.

Background technique

At present, for the prevention and control of diseases that are prone to resistance in agriculture, it is the best choice to use pesticide varieties with different action mechanisms for mixing. The effect of a single dose. Fungicides containing a single active ingredient often have certain defects in the prevention and control of agricultural diseases. Continuous repeated use not only makes pathogenic bacteria prone to drug resistance, but also easily causes pollution to food and the environment. Reasonable mixing of active ingredients in fungicides The above disadvantages can be overcome. The synergistic effect of the active ingredients through reasonable compounding can improve the control effect, reduce the amount of active ingredients, save costs, delay the emergence of drug resistance of pathogenic bacteria, and then reduce or even avoid the pollution of food and the environment by pesticides.

With regard to pesticide activity, especially for crop protection, one of the central problems of research carried out in this technical field is the improvement of performance, especially with regard to biological activity and the maintenance of this activity over a certain period of time.

Picarbutrazox, test code NF-171; CAS: 500207-04-5; chemical formula is [6-[[[[(z)-(1-methyl-1H-tetrazol-5-yl)(phenyl)methylene Base] amino] oxy] methyl] pyridin-2-yl] tert-butyl carbamate; the molecular structural formula is:

Picarbutrazox is an oxime ether fungicide developed by Nippon Soda Co., Ltd., which has a good control effect on downy mildew and blight. Picarbutrazox is known from CN02817805.X.

Picoxystrobin, its chemical name is: (E)-3-methoxy-2-[2-[6-(trifluoromethyl)-2-pyridyloxymethyl]phenyl]methyl acrylate ; Its structural formula is:

Picoxystrobin is a mitochondrial respiration inhibitor, which inhibits mitochondrial respiration through electron transfer between cytochrome b and C1. Once taken up by the leaves, picoxystrobin moves in the xylem, following the water flow in the transport system; it also flows in the gas phase on the leaf surface and flows in the xylem after being absorbed into the leaves from the gas phase. Picoxystrobin is effective against strains resistant to 1,4-demethylase inhibitors, benzamides, tricarboxamides and benzimidazoles. Picoxystrobin has a broad spectrum of control objects, and is mainly used to control wheat foliar diseases such as leaf blight, leaf rust, glume blight, brown spot, powdery mildew, etc. Compared with other methoxyacrylate fungicides Compared with, picoxystrobin has a stronger therapeutic effect on wheat leaf spot disease, net spot disease and moire disease.

Trifloxystrobin (Trifloxystrobin), the Chinese name is also trifloxacin or trifluoromin, and its chemical name is (2Z)-2-methoxyimino-2-[2-[[1-[3-(trifluoro Methyl) phenyl] ethylene amino] oxymethyl] phenyl] methyl acetate, its structural formula is:

Trifloxystrobin is a new class of fluorine-containing fungicides successfully developed from natural products Strobilurins as fungicide lead compounds. Trifloxystrobin is a respiratory chain inhibitor, which prevents the synthesis of ATPase ATP by locking the electron transfer between cytochrome b and C1, thereby inhibiting its mitochondrial respiration and exerting an antibacterial effect. It has chemical kinetic characteristics fungicide. Trifloxystrobin can be strongly adsorbed by the plant wax layer, providing excellent protective activity on the plant surface. Trifloxystrobin has the characteristics of high efficiency, broad spectrum, protection, treatment, eradication, penetration, systemic activity, and rapid distribution. The crops absorb quickly, and it has upward systemic properties, so it has good rain erosion resistance and long-lasting effect , so it is considered as a second-generation methoxyacrylate fungicide. Trifloxystrobin is effective for strains resistant to 1,4-demethylase inhibitors, benzamides, dicarboxylamines and benzimidazoles, and has no cross-resistance with existing fungicides. Trifloxystrobin is effective against almost all fungal diseases (Ascomycetes, Basidiomycetes, Oomycetes and Deuteromycetes) such as powdery mildew, rust, glume blight, net spot, downy mildew, rice blast, etc. good activity. Trifloxystrobin not only has special effects on powdery mildew and leaf spot, but also has good activity on rust, downy mildew, blight and apple black blight. Trifloxystrobin is mainly used for the treatment of stems and leaves. It has excellent protective activity and certain therapeutic activity, and the activity is not affected by the environment. The best application period of trifloxystrobin is spore germination and initial stage of pathogenesis, but it is active against scab in all stages.

The activity of the Picarbutrazox, picoxystrobin, trifloxystrobin compounds was good but sometimes unsatisfactory at low application rates. Due to the continuously increasing environmental and economic demands placed on fungicides today, such as requirements on activity spectrum, toxicity, selectivity, application rates, residues and favorable manufacturing possibilities, and also due to possible problems such as drug resistance. Therefore, it is an ongoing task to develop new fungicides which are superior in some respects to existing fungicides.

Contents of the invention

It is an object of the present invention to provide a fungicidal composition which is effective in reducing the application rate and improving the spectrum of activity of the known compounds Picarbutrazox and trifloxystrobin or picoxystrobin at a reduced total amount of active compound applied. The total amount of active compounds applied has improved activity against harmful fungi (synergism).

We have found that simultaneous, ie joint or separate application of Picarbutrazox and trifloxystrobin or picoxystrobin or sequential application of Picarbutrazox and trifloxystrobin or picoxystrobin gives better control of harmful fungi than application of the individual compounds alone.

The present invention provides a bactericidal composition, the composition is binary compounded with Picarbutrazox and trifloxystrobin or picoxystrobin, so that the obtained composition has a gain effect on the control effect, and expands the bactericidal spectrum, It has played the role of one drug with multiple functions, effectively slowing down or avoiding the emergence of drug resistance of bacteria. Surprisingly, the fungicidal activity of the fungicidal compositions according to the invention is significantly higher than the sum of the activities of the individual active compounds. In other words, there are unforeseen, real synergistic effects, not mere addition of activity.

The invention provides a fungicidal composition with higher activity and longer-lasting activity. The bactericidal composition has a lower dose, and at the same time, the toxicity of the bactericidal composition is also lower, so as to control the fruits, vegetables and seeds with fungal diseases.

The synergistic effect is particularly pronounced when the active compounds are present in the fungicidal composition of the invention in specific weight ratios. However, the weight ratio of the active compounds in the fungicidal composition of the present invention can vary within a certain range.

A kind of bactericidal composition of the present invention is to take the following technical solutions to realize:

A bactericidal composition, characterized in that: it contains active ingredients Picarbutrazox and trifloxystrobin or picoxystrobin, wherein the weight percentage of Picarbutrazox and trifloxystrobin or picoxystrobin is 50:1-1:50, preferably 25 :1-1:25, 10:1-1:10, more preferably 5:1-1:5.

The weight ratio of Picarbutrazox and trifloxystrobin or picoxystrobin in the present invention can also be 50:1, 45:1, 40:1, 35:1, 30:1, 25:1, 20:1, 15:1, 10:1, 5:1, 1:1, 1:5, 1:10, 1:15, 1:20, 1:25, 1:30, 1:35, 1:40, 1:35 45. 1:50.

The sum of the mass of Picarbutrazox and trifloxystrobin or picoxystrobin accounts for 5%-90% of the mass of the fungicidal composition, more preferably 10%-80%, more preferably 20%-60%.

A method for preventing and controlling plant pathogenic bacteria, using a fungicidal composition to act on pathogenic bacteria and/or their environment, or plants, plant parts, seeds, soil, areas, materials or spaces.

A method for preventing and controlling plant pathogenic bacteria. Picarbutrazox and trifloxystrobin or picoxystrobin are applied simultaneously, or applied separately, or applied sequentially.

A bactericidal composition comprising Picarbutrazox and trifloxystrobin or picoxystrobin and filler and/or surfactant.

A bactericidal composition, which can be formulated into any dosage form allowed in agriculture. The dosage forms of the bactericidal composition are suspensions, seed coatings, suspoemulsions, wettable powders, water dispersible granules, microcapsule suspensions, coated granules, extruded granules, emulsifiable concentrates, microemulsions, water emulsions , effervescent tablets, ultra-low volume liquid.

The fungicidal composition is used for controlling fungi and bacteria on cereals, vegetables, alfalfa, soybeans, turf, wood, trees, fruit trees or horticultural plants.

Use of the fungicidal composition for protecting plant propagation material and subsequently grown plant organs.

The bactericidal composition is used for preventing and controlling diseases of fruits and vegetables during storage.

The application of the fungicidal composition to the site where control is required is used to control soil pathogenic or saprophytic fungi and bacteria.

A method for controlling phytopathogenic fungi of plants, plant parts, plant propagation material and subsequently grown plant organs, the method comprising treating the seeds, Application by foliar application, stem application, drenching, dripping, pouring, spraying, spraying, dusting, spreading or fuming to plants, plant parts, plant propagation material or soil or cultivation in which plants are growing or are to be grown medium.

The fungicidal composition is effective on various crop plants such as bananas, cotton, vegetable varieties (such as cucumbers, beans, tomatoes and cucurbits), barley, grasses, oats, coffee, potatoes, corn, fruit varieties, rice, Control of a large number of fungi is especially important in rye, soybeans, vines, wheat, ornamentals, sugar cane, and a wide variety of seeds.

The bactericidal composition of the present invention has strong activity against various plant pathogenic bacteria, and can exert a strong control effect on the prevention and treatment of plant diseases caused by plant pathogenic bacteria. The fungicidal composition of the present invention has an excellent activity against a wide range of phytopathogenic fungi such as Pluzomycetes, Oomycetes, Chytridiomycetes, Accidiomycetes, Ascomycetes, Basidiomycetes, and Deuteromycetes.

The fungicidal composition of the present invention also has very good bactericidal properties and can be used to control phytopathogenic bacteria. Such as Pseudomonas, Rhizobium, Enterobacteriaceae, Corynebacteriaceae, and Streptomycetaceae.

The fungicidal composition of the invention can be used as a foliar fungicide in crop protection, and can also be used as a fungicide for seed dressing and as a soil fungicide.

The bactericidal combination of the present invention can also be used to prevent and control diseases in the storage period of fruits and vegetables.

The fungicidal composition of the present invention is especially suitable for controlling the following plant pathogenic bacteria:

As Oomycetes, can be exemplified, for example, Pythium genus of various crops such as sugar beet blight fungus (Pythium ultium); as Phytophthora fungus of potato (PhytophthoraiNfestaNs), tomato Botrytis cinerea (Phytophthoracapsici) Phytophthora bacterium; Such as cucumber downy mildew (PseudoperoNospora cubeNsis), law grass downy mildew (PseudoperoNospora humuli) pseudoperonospora; such as grape downy mildew (Plasmoparaviticola) uniaxial fungus; such as downy mildew of cruciferous vegetables Peronospora genus PeroNospora brassicae, onion downy mildew (PeroNospora destructor), spinach downy mildew (PeroNospora spiNaciae), etc.

Ascomycetes, for example, bacteria of the genus Erysiphe such as Erysiphegrami Nis; bacteria of the genus Monotifria such as Sphaerotheca fuligi Nea; bacteria such as Venturia iNaequalis , pear scab (VeNturia Nashicola) of the genus Venturia; such as the genus PyreNophora of barley net spot fungus (PyreNophorateres); such as the genus Cochliobolus of wheat spot disease (Cochliobolus sativus); such as vegetable Sclerotinia sclerotiorum ( SclerotiNia sclerotiorum) Sclerotinia bacteria etc.

As Basidiomycetes, there can be exemplified, for example, bacteria of the genus Bisporus such as wheat leaf rust (Pucci Niareco Ndita); bacteria of the genus Tilletia such as Tilletia caries; bacteria such as Tilletia caries; Ustilago Nuda bacteria, etc.

As the subphylum Deuteromycota, there can be exemplified, for example, Phomaasparagi such as Phomaasparagi; Septoria such as Septoria Nodorum; Septoria such as melon anthracnose Colletotrichum lageNarium, such as Pyricularia; such as Pyricularia oryzae, such as Botrytis ciNerea; such as AlterNaria mali, Alternaria such as AlterNaria solaNi; Cercospora such as Cercospora beticola; Cladosporium carpophilum such as Cladosporium carpophilum; Bacteria of the genus Rhizoctonia of RhizoctoNia solaNi, etc.

Suitable crops mainly include field crops such as corn, soybean, cotton, canola oilseeds such as Brassica napus (e.g. canola), turnip (Brassica rapa), mustard (B. juncea ) (such as mustard) and Ethiopian mustard (Brassica carinata), rice, wheat, sugar beet, sugar cane, oats, brown wheat, barley, millet, triticale, flax, vines and fruit or vegetable crops of various plant classes , such as Rosaceae sp. (for example, pome fruit such as apples and pears, but also stone fruits such as apricots, cherries, almonds and peaches, berries such as strawberries), Ribesioidae sp., walnuts Juglandaceae sp., Betulaceae sp., Anacardiaceae sp., Fagaceae sp., Moraceae sp., Oleaceae sp., Actinidaceae sp.), Lauraceae sp., Musaceae sp. (eg banana trees and plantings), Rubiaceae sp. (eg coffee), Theaceae sp., Sterculiceae sp., Rutaceae sp. (such as lemons, oranges, and grapefruit); Solanaceae sp. (such as tomatoes, potatoes, peppers, eggplants), Liliaceae sp., Compositiae sp. (for example lettuce, artichoke and chicory - including root chicory, endive or common chicory), Umbelliferae sp. (for example carrot, parsley, celeriac, and celeriac), Cucurbitaceae sp. (such as cucumbers—including pickling cucumbers, pumpkins, watermelons, gourds, and melons), Alliaceae sp. (such as onions and leeks Onions), Cruciferae (Cruciferae sp.) (such as white cabbage, red cabbage, broccoli, cauliflower, Brussels sprouts, bok choy, kohlrabi, radish, horseradish, kale, Chinese cabbage), legumes (Leguminosa esp. ) (e.g. peanuts, peas and lentils - such as vine bean and fava bean), Chenopodiaceae sp. (e.g. fodder beet, spinach beet, spinach, beetroot), mallow Malvaceae (eg, okra), Asparagaceae (eg, asparagus); horticultural and forest crops; ornamental plants; and genetically modified homologues of these crops.

The bactericidal combination of the present invention is preferably for example the control of the following diseases:

Brown spot (Cercospora beticola), black root (Aphanomyces cochlloides), root rot (Thanatephorus cucumeris), leaf rot (Thanatephorus cucumeris) in "beet";

"Peanut" brown spot (Mycosphaerella arachidis), black spot (Mycosphaerellaberkeleyi);

Powdery mildew (Sphaerotheca fuliginea), downy mildew (Pseudoperonosporacubensis), creeping blight (Mycosphaerella melonis), vine blight (Fusarium oxysporum), sclerotinia (Sclerotinia sclerotiorum), gray mold (Botrytis cinerea), Anthracnose (Colletotrichumorbiculare), scab (Cladosporium cucumerinum), brown spot (Corynespora cassicola), seedling blight (Pythium debaryanam, Rhizoctonia solani Kuhn), bacterial leaf spot (Pseudomonassyringae pv. Lecrymans);

Botrytis cinerea, leaf mold (Cladosporium fulvum), late blight (Phytophthora infestans) on "tomatoes";

Gray mold (Botrytis cinerea), black blight (Corynespora melongenae), powdery mildew (Erysiphe cichoracearum), velvet spot (Mycovellosiella nattrassii) on "eggplant";

Gray mold (Botrytis cinerea), powdery mildew (Sohaerotheca humuli), anthracnose (Colletotrichum acutatum, Colletotrichum fragariae), blight (Phytophthoracactorum) of "strawberry";

"Onion" neck rot (Botrytis allii), gray mold (Botrytis cinerea), white spot leaf blight (Botrytis squamosa), downy mildew (Peronospora destructor);

Root nodule (Plasmodiophora brassicae), soft rot (Erwinia carotovora), downy mildew (Peronospora parasitica) of "cabbage";

Sclerotinia sclerotiorum, Botrytis cinerea of "lentils";

"Apple" powdery mildew (Podosphaera leucotricha), scab (Venturia inaequalis), flower rot (Monilinia mali), black spot (Mycosphaerella pomi), rot (Valsa mali), leaf spot (Alternaria mali), Red spot (Gymnosporangium yamadae), ring spot (Botryosphaeria berengeriana), anthracnose (Glomerella cingulata, Colletotrichum acutatum), brown spot (Diplocarpon mali), fly spot (Zygophiala jamaicensis), soot spot (Gloeodes pomigena);

Powdery mildew (Phyllactinia kakicola), anthracnose (Gloeosporium kaki), angular leaf spot (Cercospora kaki) on "persimmon";

Brown rot (Monilinia fructicola), scab (Cladosporium carpophilum), Phomopsis sp. in "peach";

'Cherry' brown rot (Monilinia fructicola);

Gray mold (Botrytis cinerea), powdery mildew (Uncinula necator), late rot (Glomerella cingulata, Colletotrichum acutatum), downy mildew (Plasmopara viticola), black spot (Elsinoe ampelina), brown spot ( Pseudocercospora vitis), black rot (Guignardiabidwellii);

"Pear" scab (Venturia nashicola), red spot (Gymnosporangium asiaticum), black spot (Alternaria kikuchiana), ring spot (Botryosphaeria berengeriana), powdery mildew (Phyllactinia mali);

"Tea" ring spot (Pestalotia theae), anthracnose (Colletotrichum theae-sinensis);

Scab (Elsinoe fawcetti), Penicillium italicum, Green mold (Penicillium digitatum), Botrytis cinerea, Black spot (Diaporthe citri), Canker (Xanthomonas campestris pv. .Citri);

Powdery mildew (Erysiphe graminis f.sp. tritici), Scab (Gibberella zeae), Leaf rust (Puccinia recondita), Brown snow rot (Pythium iwayamai), Red snow rot (Monographella nivalis), Wheat on "Wheat" Pseudocercosporella herpotrichoides, Septoria tritici, Leptosphaeria nodorum, Typhulaincarnata, Myriosclerotinia borealis, Gaeumanomycesgraminis );

Stripe (Pyrenophora graminea), cloud spot (Rhynchosporium secalis), loose smut (Ustilago tritici, U. nuda) of "barley";

Rice blast (Pyricularia oryzae), sheath blight (Rhizoctonia solani), bakanae (Gibberella fujikuroi), flax leaf spot (Cochliobolus niyabeanus), seedling blight (Pythium graminicola), bacterial blight (Xanthomonas oryzae), bacterial blight (Burkholderia plantarii), brown streak (Acidovorax avenae), grain blight bacterial venereal disease (Burkholderia glumae)

"Tobacco" Sclerotinia sclerotiorum, Powdery mildew (Erysiphe cichoracearum);

'Tulip' gray mold (Botrytis cinerea);

Sclerotinia borealis and Pythiumaphanidermatum of "Western Zoysia";

'Wild grass' powdery mildew (Erysiphe graminis);

Purple spot (Cercospora kikuchii), downy mildew (Peronospora Manshurica), stem blight (Phytophthora sojae) on "soybean";

Potato and tomato late blight (Phytophthora infestans).

The fungicidal composition of the present invention can well control the pathogens and drug-resistant bacteria that cause the following diseases: rice seedbed blight and bakanae disease; cotton seedling diseases such as blight, anthracnose, damping-off, red rot Vegetable seedbed diseases such as damping-off anthracnose, blight, tomato phytophthora, gray mold, leaf rust, spot disease, anthracnose and early phytophthora; cucumber downy mildew, powdery mildew, gray mold blight, anthracnose, blight, black spot and brown spot; strawberry anthracnose and gray mold; pumpkin powdery mildew, anthracnose and blight; onion white spot, black spot, gray Mildew and gray rot; powdery mildew, gray mold and black blight of eggplant; black spot of scallions; white spot and black spot of cabbage; sclerotinia and gray mold of bell peppers; powdery mildew, Anthracnose and blight; powdery mildew, anthracnose and blight of melon; gray mold of lettuce; black spot and gray mold of bayberry; powdery mildew, anthracnose, gray mold and defoliation of persimmon; Botrytis of citrus; scab, black spot, and early blight of pears; botrytis, anthracnose, brown spot, black vine, dead vine, sticky blight, and branch bloat of grapes; Scab, gray spot, anthracnose; apple scab, ring spot, early blight, brown spot, coal spot, coal spot, black spot, and brown rot; mung bean stem blight anthracnose, gray mold and sclerotinia; bean anthracnose, gray mold and sclerotinia; broad bean ring spot; soybean purple spot; tobacco powdery mildew, sugar beet brown spot and spot; Anthracnose, ring spot, bud dieback and gray mold of tea; plant diseases such as scab, anthracnose, gray mold and powdery mildew of flowers. The fungicidal composition of the present invention is especially capable of preventing and controlling plant pathogenic bacteria and drug-resistant bacteria that cause blight, powdery mildew and gray mold of tomato, cucumber, eggplant, strawberry, grape, peach and citrus.

The fungicidal composition of the present invention is especially suitable for controlling downy mildew, early blight, late blight, black blight, and Shing disease, scab, leaf spot, anthracnose, powdery mildew, wilt, blight, damping-off.

The crops treated by the fungicidal composition of the present invention are, for example but not limited to, cereals, vegetables, alfalfa, soybeans, turf, wood, trees, fruit trees or horticultural plants.

The bactericidal mixture of the invention can also be used to prevent and control diseases of fruits and vegetables during storage. Examples include fruit rot caused by:

Aspergillus species, such as Aspergillus flavus;

Botrytis species, such as Botrytis cinerea;

Penicillium species such as Penicillium expansum and P. purpurogenum;

Sclerotinia species such as Sclerotinia sclerotiorum;

Verticilium species such as Verticilium alboatrum.

The fungicidal composition of the present invention is also particularly suitable for preventing and controlling diseases such as stalk rot, green mold, penicillium and anthracnose during storage of fruits and vegetables.

The fungicidal mixtures of the present invention can also be used to control seed-borne and soil-borne diseases. Examples are seed-borne and soil-borne rot and wilt diseases and seedling diseases caused by the following pathogens:

Alternaria species such as Alternaria brassicicola;

Aphanomyces (Aphanomyces) genus species, such as Aphanomyces beanus (Aphanomyces

euteiches);

Ascochyta genus species, such as Ascochyta lentis;

Aspergillus, such as Aspergillus flavus;

Cladosporium species such as Cladosporium herbarum;

Coelomyces spp., such as Coelomyces graminearum;

(conidia form: Delburia, Syn: Helminthosporium);

Anthracnose species such as Colletotrichum coccodes;

Fusarium species, such as Fusarium yellow;

Gibberella species, such as Gibberella macula;

Species of the genus Macrophomina, such as Macrophomina

phaseolina);

Echinococcus species, such as Echinococcus nivalis;

Penicillium species, such as Penicillium expanse;

Phaeosphaeria species such as Phaeosphaeria nodorum;

Phoma genus species, such as Phoma lingam;

Phomopsis, such as Phomopsis sojae;

Phytophthora species, such as Phytophthora cactorum;

Pyrenophora graminea species, such as Pyrenophora graminea;

Pyricularia species, such as Pyricularia oryzae;

Pythium species, such as Pythium ultima;

Rhizoctonia species, such as Rhizoctonia solani;

Rhizopus (Rhizopus) genus species, such as Rhizopus oryzae (Rhizopus oryzae);

Sclerotium genus species, such as Sclerotium rolfsii ;

species of the genus Typhula, such as Typhula incarnata ;

Verticillium genus species, for example Verticillium dahliae.

The bactericidal composition of the present invention has an excellent bactericidal effect even on bacteria that have shown resistance to conventional bactericides. Examples include Botrytis cinerea, Cercospora beticola, Venturia inaequalis, Venturia nashicola, etc.

A method for controlling phytopathogenic fungi in plants, plant parts, plant propagation material and subsequently grown plant organs, comprising treating seeds, leaves, and leaves with an agronomically effective and substantially non-phytotoxic application rate Application to plants, plant parts, plant propagation material, or the soil or growing medium in which the plant is growing or is to be grown by surface application, stem application, drenching, dripping, pouring, spraying, spraying, dusting, spreading or fuming middle.

The fungicidal composition of the present invention can be used for the prevention and treatment of various diseases that occur during the cultivation of agricultural and horticultural crops including flowers, lawns, pastures, etc. by seed treatment, stem and leaf spraying, soil application or water surface application.

The invention provides a method for preventing and controlling plant pathogenic bacteria. The fungicidal composition acts on the pathogenic bacteria and/or its environment, or plants, plant parts, plant propagation materials, soil, areas, materials or spaces.

The fungicidal compositions according to the invention can be used for the protection of plant parts, plant propagation material and subsequently grown plant organs.

The fungicidal composition of the present invention can treat all plants and plant parts. "Plant" refers to all plants and plant populations, such as desirable and undesirable wild plants, cultivated plants and plant varieties (whether or not protected by plant variety or plant breeder's rights). Cultivated plants and plant varieties may be plants obtained by conventional methods of propagation and breeding, which may be supplemented or complemented by one or more biotechnological methods, such as the use of double haploids, protoplast fusion, random and directed mutagenesis, molecular Or genetic markers, or using bioengineering and genetic engineering methods. Plant parts mean all above-ground and underground parts and organs of plants, such as buds, leaves, flowers and roots, such as leaves, needles, stems, branches, flowers, fruiting bodies, fruits and seeds and roots, bulbs and rhizomes. Crops and vegetative and generative propagation material, such as cuttings, bulbs, rhizomes, runners and seeds, also belong to plant parts.

The term "plant propagation material" is understood to mean all reproductive plant parts, such as seeds, which can be used to propagate the latter, as well as vegetable material such as cuttings or tubers (eg potatoes). Thus, plant parts as used herein include plant propagation material. There may be mentioned, for example, seeds, roots, fruits, tubers, bulbs, rhizomes and plant parts. Germinated plants and effective plants to be inhibited after germination from soil or after emergence. Young plants can be protected by total or partial treatment by dipping before transplanting.

The preferred propagation material according to the invention is seeds. Seed treatment methods include, for example, diluting a liquid or solid drug or soaking the seed in a liquid solution without dilution to allow the drug to permeate the seed, mixing a solid drug or a liquid drug with the seed, and wrapping the seed. The method of coating the surface of the seed to attach the chemical agent, spraying near the seed while planting, etc.

Plant parts and subsequently grown plant organs are any part of a plant produced from plant propagation material such as seeds. Plant parts, plant organs and plants can also benefit from the protection from pathogen damage obtained by applying the fungicidal composition to plant propagation material. Certain plant parts and plant organs grown on certain loci may also be considered as plant propagation material, which may itself be applied (or treated) with a fungicidal composition; Plants, other plant parts and other plant organs may also benefit from application of the fungicidal composition.

The fungicidal composition of the present invention can also be used to prevent or control various pathogenic or saprophytic fungi and bacteria in soil or cultivation medium. Examples of soil-borne fungal pathogens include Alternaria spp., Ascochyta spp., Botrytis cinerea, Cercospora spp., Claviceps purpurea , Cochliobolus sativus, colletotrichumspp., Epicoccum spp., Fusarium graminearum, Fusarium moniliforme, Fusarium oxysporum (Fusarium oxysporum, Fusarium proliferatum, Fusarium solani, Fusarium subglitinans, Helminthosporium spp, Microdochium nivale, Penicillium Pencillium spp, Phoma spp., Pyrenophora graminea, Pyricularia oryzae, Rhizoctonia solani, Rhizoctonia graminea (Rhizoctonia cerealis), Sclerotinia spp., Septoria spp., Sphacelotheca reilliana, Tilletia spp., Sclerotinia spp. (Typhulaincarnate), Urocystis occulta, Ustilago spp. or Verticillium spp.

Soil pathogens include blight, fusarium, phytophthora, damping-off fungus, root rot, pythium, gray mold, soft rot and so on. Under normal circumstances, soil pathogens can produce a large number of bacteria. As long as the conditions are favorable for the growth and development of the bacteria and the host is susceptible, the bacteria can reproduce in large numbers and infect the host. In the presence of a susceptible host, these bacteria can Entering the continuous pathogenic period, with the continuous cropping of crops, they will multiply and spread in large numbers, but then when the nutrients are exhausted or the soil conditions such as temperature and humidity are unfavorable to the bacteria, the bacteria can enter the dormant period again. In the absence of susceptible hosts, soil-borne pathogens can also survive in the soil. In addition to soil pathogens having a wide range of hosts, they can also survive on non-host root surfaces or residual branches and leaves. Its saprophytic competition ability is Inseparable. But different pathogens are different, like Fusarium can survive almost indefinitely in the soil.

The cultivation medium of the present invention refers to the support body that can make the crops take root and grow, such as: soil, water, etc. The specific raw materials can be sand, pumice, vermiculite, diatomaceous earth, agar, gel , polymer substances, asbestos, sawdust, bark, etc.

The method of applying the medicament to the soil, such as diluting the liquid medicament in water or directly applying it to the root of the plant or the seedling field for raising seedlings without dilution, and spreading the granule to the root of the plant or the seedling field for raising seedlings. The method of spraying powder, water-dispersible granules, etc. in the soil before sowing and mixing them with the soil as a whole, before sowing or planting plants, dilute the powder and water-dispersible granules and spray them on the planting holes and sowing ditch. method of sowing, etc.

Another object of the present invention is to provide a method of controlling phytopathogenic fungi in plants, plant parts, plant propagation material and subsequently grown plant organs, the method comprising applying the fungicidal composition of the present invention in an agronomically effective and essentially plant-free Toxic application rates are applied by seed treatment, foliar application, stem application, drenching, dripping, pouring, spraying, spraying, dusting, scattering or fuming to plants, plant parts, plant propagation material or plants that are growing or Requires the soil or growing medium in which to grow.

The fungicidal composition of the present invention can be applied by different treatment methods, such as:

- spraying a liquid comprising said fungicidal composition onto the aerial parts of said plants;

- dusting, incorporating granules or powders into the soil, spraying around said plants, and in the case of tree injection or smearing;

- Coating or film coating of the seeds of plants.

- When it is used for post-harvest antiseptic preservation of fruits and vegetables, it is usually diluted 200-2000 times with water, soaked and drained.

The invention provides a method for preventing and controlling plant pathogenic bacteria, which can be a treatment, prevention or eradication method.

The fungicidal composition of the present invention can be made into common pharmaceutical forms, such as emulsifiable concentrate, wettable powder, suspension, liquid, granule, seed coating and other pharmaceutical forms. Conditions, dosage form, application period, application method, application site, control target pests, target crops, etc. vary.

A method for preventing and controlling plant pathogenic bacteria. Picarbutrazox and trifloxystrobin or picoxystrobin are applied simultaneously, or applied separately, or applied sequentially.

Treatment according to the invention may produce super-additive ("synergistic") effects. For example, according to the application rate of the fungicidal composition used according to the invention and/or broadening its activity spectrum and/or increasing its activity, it is possible to obtain the following effects: better plant growth, increased tolerance to high or low temperatures, Increased tolerance to drought or water or soil salt content, improved flowering performance, easier harvesting, accelerated ripening, higher yield, larger fruit, higher plant height, greener leaf color, better flowering Benefits of earlier, higher quality or nutritional value of the harvested product, higher sugar concentration in the fruit, better storage stability and/or processability of the harvested product outweighed the actual projected effects.

The treatment method according to the invention can also be used for the treatment of propagation material such as tubers or rhizomes and for the treatment of seeds, seedlings or shoots pricking out and plants or plants pricking out. This treatment method can also be used to treat roots. The treatment method according to the invention can also be used for the treatment of above-ground parts of plants such as trunks, stems or stalks, leaves, flowers and fruits of the plants concerned.

- generally for foliar treatments: 0.1-10000 g/ha, preferably 10-1000 g/ha, more preferably 50-500 g/ha; When applying an inert substrate such as asbestos or perlite;

- for seed treatment: 2-5000g/100kg seed, preferably 3-1000g/100kg seed;

- for soil or surface application treatments: 0.1-10000 g/ha, preferably 1-1000 g/ha;

- For post-harvest preservation of fruits and vegetables, it can be diluted 200-2000 times, soaked and drained.

The above-mentioned doses are only general exemplary doses. During actual application, those skilled in the art will adjust the application rate according to actual conditions and needs, especially according to the nature of the plants or crops to be treated and the severity of pathogens.

Picarbutrazox according to the invention is administered in combination/association with trifloxystrobin or picoxystrobin. Including separate, sequential or simultaneous administration of Picarbutrazox and trifloxystrobin or picoxystrobin. Preferably, the combination of Picarbutrazox and trifloxystrobin or picoxystrobin is in the form of a composition comprising Picarbutrazox and trifloxystrobin or picoxystrobin.

The composition of the present invention can be mainly in the form of a preparation, that is, each substance in the composition has been mixed, and the components of the composition can also be provided in a single dose form, mixed in a barrel or tank before use, and then diluted to a desired concentration. Among them, the preparation form provided by the present invention is preferred.

As a further improvement of the present invention, the fungicidal composition of the present invention can be formulated into any dosage form acceptable in agriculture.

As a further improvement of the present invention, the dosage form of the fungicidal composition of the present invention is suspension concentrate, seed coating, wettable powder, water dispersible granule, microcapsule suspension, coated granule, extruded granule, emulsifiable concentrate, microencapsulation Emulsion, emulsion in water, effervescent tablet, ultra-low volume liquid, suspoemulsion.

The bactericidal composition of the present invention contains Picarbutrazox, trifloxystrobin or picoxystrobin, filler and/or surfactant.

In the bactericidal composition of the present invention, the content of Picarbutrazox and trifloxystrobin or picoxystrobin accounts for 5%-90% of the bactericidal composition.

The bactericidal composition, wherein the content of Picarbutrazox and trifloxystrobin or picoxystrobin accounts for 10%-80% of the bactericidal composition.

The bactericidal composition, wherein the content of Picarbutrazox and trifloxystrobin or picoxystrobin accounts for 20%-60% of the bactericidal composition.

According to the invention, the term "filler" refers to a natural or synthetic organic or inorganic compound which can be combined or associated with an active compound to make it easier to apply to a subject, such as a plant, crop or grass. Thus, the filler is preferably inert, at least agriculturally acceptable. The filler can be solid or liquid.

The non-active medium that can be used in the present invention can be solid also can be liquid, and what can be used as solid medium material has for example: plant matter powder class (such as soybean powder, starch, grain powder, wood powder, bark powder, Sawdust, walnut shell powder, bran, cellulose powder, coconut shell, corncob and tobacco stem particles, residue after extracting plant essence, etc.), paper, sawdust, synthetic aggregates such as pulverized synthetic resin, clay ( Such as kaolin, bentonite, acid china clay, etc.), talcum powder. Silica (such as diatomaceous earth, silica sand, mica, hydrous silicic acid, calcium silicate), activated carbon, natural minerals (pumice, attapulgite, zeolite, etc.), fired diatomaceous earth, sand, plastic media, etc. (such as polyethylene, polypropylene, polyvinylidene chloride, etc.), inorganic mineral powders such as potassium chloride, calcium carbonate, and calcium phosphate, chemical fertilizers such as ammonium sulfate, ammonium phosphate, urea, and ammonium chloride, and soil fertilizers, These substances may be used alone or in combination of two or more.

Those that can be used as liquid medium materials can be selected from the following materials, such as water, alcohols (such as methanol, ethanol, isopropanol, butanol, ethylene glycol, etc.), ketones (such as acetone, methyl ethyl ketone, Diisobutyl ketone, cyclohexanone, etc.), ethers (such as diethyl ether, dioxane, methylcellulose, tetrahydrofuran, etc.), aliphatic hydrocarbons (such as kerosene, mineral oil, etc.), aromatic hydrocarbons (such as benzene, toluene, xylene, mineral spirits, alkylnaphthalene, chlorinated aromatics, chlorinated aliphatic hydrocarbons, chlorobenzene, etc.), halogenated hydrocarbons, amides, sulfones, dimethyl sulfoxide, Mineral and vegetable oils, animal oils, etc.

In order to emulsify, disperse, and/or moisten the active ingredient compound, surfactants such as fatty alcohol polyoxyethylene ether, polyoxyethylene alkyl aryl ether, polyoxyethylene higher fatty acid ester, polyoxyethylene Phosphate esters of alcohols or phenols, fatty acid esters of polyols, alkanaryl sulfonic acids, naphthalene sulfonic acid polymers, lignin sulfonates, polymer comb-shaped branched copolymers, butyl naphthalene sulfonates, alkyl Aryl sulfonate, sodium alkyl sulfosuccinate, oil, fatty alcohol and ethylene oxide condensate, alkyl taurate and other polyacrylates, protein hydrolyzate. Suitable oligosaccharides or polymers are eg based on vinylic monomers, acrylic acid, polyoxyethylene and/or polyoxypropylene alone or in combination with eg (poly)alcohols or (poly)amines.

For dispersion stabilization, adhesion and/or binding of active ingredient compounds, for example, xanthan gum, magnesium aluminum silicate, gelatin, starch, cellulose methyl ether, polyvinyl alcohol, polyvinyl acetate and natural phospholipids (such as brain Phospholipids and lecithins) and auxiliary agents such as synthetic phospholipids, bentonite, and sodium lignosulfonate.

Among them, the antifreeze can be selected from ethylene glycol, propylene glycol, glycerol, and sorbitol. As the deflocculating agent for suspension products, auxiliary agents such as naphthalenesulfonic acid polymers and polymeric phosphates can be used.

Silicone defoamers can be used as defoamers.

Colorants that can be used, such as inorganic pigments such as iron oxide, titanium oxide and Prussian blue; and organic pigments/dyes: alizarin dyes, azo dyes and metal phthalocyanine dyes; and trace elements such as iron salts, manganese salts, Salts of boron, copper, cobalt, molybdenum and zinc.

Optionally, other additional components can also be included, such as protective colloids, adhesives, thickeners, thixotropes, penetrants, stabilizers, masking agents.

The formulations according to the invention can be prepared by mixing the active compounds with customary additives in a known manner. The conventional additives such as conventional extenders as well as solvents or diluents, emulsifiers, dispersants, and/or adhesives or fixatives, wetting agents, water repellents, and if necessary, driers and colorants , stabilizers, pigments, defoamers, preservatives, thickeners, water and other processing aids.

The fungicidal compositions of the present invention include not only ready-to-apply to the object to be treated by means of suitable equipment, such as spraying or dusting equipment, but also concentrated commercial compositions which need to be diluted before application to the object.

The compositions containing Picarbutrazox and trifloxystrobin or picoxystrobin according to the invention can also be administered in combination with other active ingredients, for example to broaden the spectrum of activity or to prevent the development of resistance. The other active ingredients are, for example, fungicides, bactericides, attractants, insecticides, acaricides, nematicides, growth regulators, herbicides, safeners, fertilizers or semiochemicals.

The active compound Picarbutrazox and trifloxystrobin or picoxystrobin can be applied simultaneously, or separately, or sequentially, and the order of separate applications generally has no effect on the control results.

The composition of the present invention can be mainly in the form of a preparation, that is, each substance in the composition has been mixed, and the components of the composition can also be provided in a single dose form, mixed in a barrel or tank before use, and then diluted to a desired concentration. Among them, the preparation form provided by the present invention is preferred.

The fungicidal composition of the present invention has improved activity and synergistic effect on harmful fungi when the total amount of active compounds applied is reduced. In addition, the fungicidal composition of the present invention has an excellent fungicidal effect even on bacteria exhibiting resistance to conventional fungicides.

A bactericidal composition of the present invention, the composition is binary compounded with Picarbutrazox and trifloxystrobin or picoxystrobin, so that the obtained composition has a gain effect on the control effect, and expands the bactericidal spectrum, playing a role The multi-purpose effect of one drug can effectively slow down or avoid the development of drug resistance of bacteria. The fungicidal activity of the fungicidal compositions according to the invention is significantly higher than the sum of the activities of the individual active compounds. There are unforeseen, real synergistic effects, not mere addition of activity.

The invention provides a fungicidal composition with higher activity and longer-lasting activity. The bactericidal composition has a lower dose, and at the same time, the toxicity of the bactericidal composition is also lower, so as to control the fruits, vegetables and seeds with fungal diseases.

The synergistic effect is particularly pronounced when the active compounds are present in the fungicidal composition of the invention in specific weight ratios. However, the weight ratio of the active compounds in the fungicidal composition of the present invention can vary within a certain range.

detailed description

The present invention will be further described below through specific formulation examples.

Formulation example

Embodiment 1: 40% Picarbutrazox trifloxystrobin wettable powder

Picarbutrazox 10%, Trifloxystrobin 30%, Lignosulfonate 6%, Sapindus powder 4%, Attapulgite to 100%, mix the above-mentioned ingredients, stir evenly in a stirring tank, pass through a jet mill Then mix evenly to obtain 40% Picarbutrazox·trifloxystrobin wettable powder.

Embodiment 2: 50% Picarbutrazox picoxystrobin wettable powder:

Picarbutrazox 5%, picoxystrobin 45%, naphthalene sulfonate formaldehyde condensate 8%, tea Ku 5%, kaolin to 100%, mix the above-mentioned ingredients, stir evenly in a stirring tank, pass through a jet mill Mix evenly to prepare 50% Picarbutrazox·picoxystrobin wettable powder.

Example 3: 51% Picarbutrazox · picoxystrobin wettable powder

Picarbutrazox 1%, picoxystrobin 50%, fatty alcohol polyoxyethylene ether 7%, wetting and penetrating agent F 5%, diatomaceous earth was added to 100%, the above-mentioned ingredients were mixed, stirred evenly in a stirring tank, and passed through After jet milling, mix uniformly to prepare 51% Picarbutrazox picoxystrobin wettable powder.

Example 4: 32% Picarbutrazox trifloxystrobin water dispersible granules

Picarbutrazox 30%, trifloxystrobin 2%, alkylbenzenesulfonate calcium salt 8%, pull open powder BX 6%, ammonium sulfate 2%, bentonite to 100%, mix the above ingredients evenly, and pulverize with ultra-fine airflow machine, kneaded, and then added to a fluidized bed granulation dryer for granulation, drying, sieving, after sampling analysis and mixing to obtain 32% Picarbutrazox trifloxystrobin water dispersible granules.

Example 5: 60% Picarbutrazox trifloxystrobin water dispersible granules

Picarbutrazox 20%, Trifloxystrobin 40%, Fatty Acid Polyoxyethylene Ether 7%, Sodium Lauryl Sulfate 4%, Aluminum Chloride 2.8%, Kaolin clay added to 100%, mix the aforementioned ingredients evenly, and use ultra-fine airflow Grinding machine, kneading, and then adding to a fluidized bed granulation dryer for granulation, drying, sieving, after sampling analysis and mixing to prepare 60% Picarbutrazox trifloxystrobin water dispersible granules.

Example 6: 90% Picarbutrazox · picoxystrobin water dispersible granules

Picarbutrazox 20%, picoxystrobin 70%, octylphenol polyoxyethylene ether sulfate 1%, sodium dodecylbenzenesulfonate 1%, sodium carbonate 1%, white carbon black to 100%, add the above Mix the ingredients evenly, knead with an ultra-fine airflow pulverizer, and then add them to a fluidized bed granulation dryer for granulation, drying, and sieving. After sampling and analysis, 90% Picarbutrazox·picoxystrobin water dispersion is obtained. granules.

Example 7: 45% Picarbutrazox · picoxystrobin suspension concentrate

Picarbutrazox 40%, picoxystrobin 5%, polycarboxylate 6%, silicone oil 0.3%, xanthan gum 0.9%, diethylene glycol 2.5%, deionized water to 100%, thickener and antifreeze mixed Finally, the rest of the components except the active ingredient are uniformly mixed through high-speed shearing, and the active ingredient is added, and ball milled in a ball mill for 2 to 3 hours, so that the particle size is all below 5 μm, that is, 45% Picarbutrazox picoxystrobin is prepared suspending agent.

Example 8: 55% Picarbutrazox Trifloxystrobin Suspension Concentrate

Picarbutrazox 50%, trifloxystrobin 5%, naphthalenesulfonate formaldehyde condensate 6%, hydroxyethyl cellulose 0.7%, silicone compounds 0.1%, polyethylene glycol 2%, deionized water to 100%, the After the thickener and antifreeze are mixed, the remaining components except the active ingredients are uniformly mixed through high-speed shearing, and the active ingredients are added, and ball milled in a ball mill for 2 to 3 hours, so that the particle size is all below 5 μm, that is, 55% Picarbutrazox trifloxystrobin suspension concentrate.

Example 9: 40% Picarbutrazox picoxystrobin suspension concentrate

Picarbutrazox 15%, picoxystrobin 25%, lignosulfonate 8%, aluminum magnesium silicate 1%, silicones 0.2%, glycerol 2.8%, deionized water to 100%, thickener After mixing with antifreeze, the remaining components except the active ingredients are uniformly mixed through high-speed shearing, and the active ingredients are added, and ball milled in a ball mill for 2 to 3 hours, so that the particle size is all below 5 μm, that is, 40% Picarbutrazox pyridine is prepared. Oxystrobin Suspension Concentrate.

Example 10: 20% Picarbutrazox trifloxystrobin suspoemulsion

Picarbutrazox 10%, trifloxystrobin 10%, SOLVESSO ^TM 100 10%, alkylbenzene sulfonate calcium salt 7%, methylcellulose 1.2%, propylene glycol 2.4%, C8~10 fatty alcohols 0.4%, Tween 80 7 %, add water to 100%; add finely ground suspension phase of Picarbutrazox to the continuous phase containing trifloxystrobin, and mix to prepare 20% Picarbutrazox·trifloxystrobin suspoemulsion.

Example 11: 20% Picarbutrazox·picoxystrobin suspoemulsion

Picarbutrazox 5%, picoxystrobin 15%, lignosulfonate 6%, xanthan gum 0.8%, ethylene glycol 2.5%, SOLVESSO ^TM 100 15%, silicone oil 0.2%, 600# phosphate 5%, water added to 100%. Add the suspension phase of finely ground Picarbutrazox into the continuous phase containing picoxystrobin, and mix to prepare 20% Picarbutrazox·picarbutrazox suspoemulsion.

Example 12: 30% Picarbutrazox trifloxystrobin suspoemulsion

Picarbutrazox 20%, trifloxystrobin 10%, naphthalenesulfonic acid formaldehyde condensate 7%, saponin powder 3%, phenolic resin 1.1%, triethylene glycol 2%, silicone 0.1%, agricultural milk 400#6%, water Add to 100%, add finely ground Picarbutrazox suspension phase to the continuous phase containing trifloxystrobin, and mix to prepare 30% Picarbutrazox · Trifloxystrobin suspoemulsion.

Example 13: 10% Picarbutrazox trifloxystrobin microemulsion

Picarbutrazox 2%, trifloxystrobin 8%, N-methylpyrrolidone 6%, ethylene oxide-propylene oxide block copolymer 4%, alkyl diphenyl ether disulfonic acid magnesium salt 5%, silicone compounds 0.4%, toluene 3%, propylene glycol 2%, epichlorohydrin 1.1%, and deionized water were added to 100% to make 10% Picarbutrazox trifloxystrobin microemulsion.

Example 14: 5% Picarbutrazox picoxystrobin microemulsion

Picarbutrazox 1%, picoxystrobin 4%, cyclohexanone 5%, agricultural milk 500#4%, agricultural milk 700#5%, C8~10 fatty alcohols 0.2%, ethyl acetate 2.5%, polyethylene glycol 2%, tributyl phosphate 1.4%, and deionized water were added to 100% to prepare 5% Picarbutrazox picoxystrobin microemulsion.

Example 15: 50% Picarbutrazox · trifloxystrobin water dispersible granules

Picarbutrazox 40%, trifloxystrobin 10%, octylphenol polyoxyethylene ether sulfate 1%, sodium dodecylbenzene sulfonate 1%, sodium carbonate 1%, white carbon black added to 100%, the aforementioned formula Mix the materials evenly, knead with an ultra-fine jet mill, and then add them to a fluidized bed granulation dryer for granulation, drying, and sieving. After sampling and analysis, 50% Picarbutrazox trifloxystrobin water-dispersible granules are obtained. .

Example 16: 10% Picarbutrazox Trifloxystrobin Water Emulsion

Picarbutrazox 5%, Trifloxystrobin 5%, Phosphate 600# 4%, Propanol 7%, Agricultural Milk 600# 3%, Xanthan Gum 0.7%, Propylene Glycol 2.4%, Toluene 4%, Deionized water added to 100% , made of 10% Picarbutrazox trifloxystrobin aqueous emulsion.

Example 17: 11% Picarbutrazox Trifloxystrobin EC

Picarbutrazox 1%, trifloxystrobin 10%, ethoxylated castor oil 5%, calcium dodecylbenzenesulfonate 3%, SOLVESSO ^TM 200 added to 100%, stirred until a clear homogeneous phase was obtained to obtain 11% Picarbutrazox · Trifloxystrobin EC.

Example 18: 30% Picarbutrazox·picoxystrobin EC

Picarbutrazox 5%, picoxystrobin 25%, ethoxylated castor oil 5%, calcium dodecylbenzenesulfonate 3%, SOLVESSO ^TM 200 add to 100%, stir until a clear homogeneous phase is obtained to obtain 30% Picarbutrazox picoxystrobin EC.

Example 19: 20% Picarbutrazox Trifloxystrobin Microcapsule Suspension-Suspension Concentrate

Picarbutrazox 10%, trifloxystrobin 10%, Synperonic PE/64 15%, citric acid 0.05%, water 10%, PAPI 20%, SOLVESSO ^TM 100 5%, dispersant LFH 0.15%, defoamer 0.16%, urea 5.5%; make up to 100% with water; add the oil phase formed by PAPI, trifloxystrobin and SOLVESSO ^TM 100 to the aqueous solution containing Synperonic PE/64 to form an emulsion. Then heat and keep warm at 50° C. and add catalyst to react for 2 hours. After cooling, microcapsules of trifloxystrobin were obtained. Synperonic PE/64, dispersant LFH, defoamer, urea, Picarbutrazox and water are mixed evenly in proportion, and sand milled to prepare a suspension. Add the obtained trifloxystrobin microcapsules into the suspension concentrate of Picarbutrazox, and stir evenly to obtain a 20% suspension-suspension concentrate of Picarbutrazox·trifloxystrobin microcapsules.

Example 20 35% Picarbutrazox trifloxystrobin seed coating agent

Picarbutrazox 30%, trifloxystrobin 5%, fatty alcohol polyoxyethylene ether monosodium sulfosuccinate 10%, modified calcium lignosulfonate 5%, xanthan gum 1%, bentonite 1%. Glycerol 5%, PVP-K30 1%, make up to 100%, the above components are mixed uniformly in proportion, and sand-milled to prepare 35% Picarbutrazox·trifloxystrobin seed coating agent.

Example 21 50% Picarbutrazox · trifloxystrobin coated granules

Picarbutrazox 10%, Trifloxystrobin 40%, Polyethylene Glycol 3%, Highly Dispersed Silicic Acid 1%, Calcium Carbonate to 100%. In this way, 50% Picarbutrazox trifloxystrobin dust-free coated granules can be obtained on a carrier moistened with ethylene glycol.

Example 22 Picarbutrazox 40% and trifloxystrobin 60% were mixed evenly.

Example 23 Picarbutrazox 60% and picoxystrobin 40% were mixed evenly.

Above embodiment proportioning is weight percentage proportioning.

Biological test case:

Combining the active ingredients of different pesticides to make pesticides is an effective and fast way to develop and develop new pesticides and prevent and control resistant pathogens in agriculture. When pesticides of different varieties are mixed, they usually show three types of effects: additive effect, synergistic effect and antagonistic effect. However, it is impossible to predict the specific role, and it can only be known through a large number of experiments. The compound and synergistic formula can significantly improve the actual control effect and reduce the use of pesticides, thus greatly delaying the emergence of resistance.

1. Toxicity test:

According to Sun Yunpei's method, the toxicity index of each agent and the co-toxicity coefficient (CTC value) of the mixture are calculated. When CTC ≤ 80, the composition exhibits antagonistic effect; when 80<CTC<120, the composition exhibits additive effect. effect, when CTC ≥ 120, the composition exhibits a synergistic effect.

Actual Toxicity Index (ATI)=(standard drug EC50/test drug EC50)*100

Theoretical Toxicity Index (TTI) = Toxicity Index of Agent A * Percentage of A in the Mixture + Toxicity Index of B Agent * Percentage of B in the Mixture

Co-toxicity coefficient (CTC)=[mixture measured toxicity index (ATI)/mixture theoretical toxicity index (TTI)*100

Experiment 1: Toxicity determination of cucumber downy mildew

Cucumber seedlings with consistent growth were selected, sprayed with a potter spray tower at a pressure of 50PSI, about 5mL per pot, and 12 concentration gradients were set for each agent. Inoculation was carried out 24 hours after the chemical treatment, and conidia were evenly shaken off the leaves of cucumber downy mildew collected from the field above the cucumber seedlings for inoculation, and then the cucumber seedlings were cultured in the greenhouse. After 7 days, the disease index of the whole plant was investigated according to the incidence grading standard of cucumber downy mildew, and the control effect was calculated, and then the inhibitory intermediate concentration EC50 was calculated by the least square method, and the co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei method.

Table 1

As can be seen from Table 1, when Picarbutrazox and trifloxystrobin or picoxystrobin are in the weight ratio range of 50:1-1:50 for the prevention of cucumber downy mildew, the co-toxicity coefficient is greater than 120, indicating that both are in this range The mixing within is manifested as a gain effect.

Experiment 2: Virulence determination of tomato blight

Tomato seedlings with consistent growth were selected, and 3 pots of test leaf seedlings were selected for each treatment, sprayed with a potter spray tower at a pressure of 50PSI, about 5mL per pot, and 12 concentration gradients were set for each agent. Inoculation was carried out 24 hours after the chemical treatment, and the conidia were evenly shaken off the tomato seedlings from the tomato blight leaves collected from the field to inoculate, and then the tomato seedlings were cultured in the greenhouse. After 7 days, the disease index of the whole plant was investigated according to the classification standard of tomato blight, and the control effect was calculated, and then the inhibitory intermediate concentration EC50 was calculated by the least square method, and the co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei method.

Table 2: The virulence test result of the present invention on the prevention and treatment of tomato blight

As can be seen from Table 2, when Picarbutrazox and trifloxystrobin or picoxystrobin are in the scope of the weight proportion 50:1-1:50 of preventing tomato blight, the co-toxicity coefficient is all greater than 120, shows that both are in this range. Mixing is manifested as a gain effect.

Experiment 3: Virulence determination of citrus penicillium

Using the method of inhibiting mycelial growth rate: the test target is Penicillium citrus.

Dissolve Picarbutrazox, trifloxystrobin or picoxystrobin in acetone respectively, then dilute with 0.1% Tween-80 aqueous solution to prepare a series of medicinal solutions, draw 6 mL into sterilized Erlenmeyer flasks in the ultra-clean workbench, Add 54mL of potato dextrose agar medium (PDA) at about 50°C, shake well and pour into 4 plates with a diameter of 9cm to make 4 toxic medium with corresponding concentrations; use the same method to mix different ratios of Picarbutrazox and Trifloxystrobin or picoxystrobin series concentration compound solution is used to make toxic culture medium. Cultivate Penicillium citrus for 2 days, use a hole puncher with a diameter of 5mm to form a bacterial block on the edge of the colony, use an inoculation needle to move the bacterial block to the center of the pre-prepared toxic PDA medium, and then place it at 25°C for cultivation Cultured in the box, each treatment was repeated 4 times. After 3 days, the colony diameter cm of each treatment was measured with a caliper by the cross method, and the corrected inhibition percentage was calculated. Two diameters of each colony were measured in a cross, and the average number represented the colony size. Then calculate the colony growth inhibition rate according to the following formula:

Then use the least square method to calculate the inhibitory concentration EC ₅₀ , and then calculate the co-toxicity coefficient (CTC) according to Sun Yunpei's method.

Table 3: Results of virulence tests against citrus penicillium

It can be seen from Table 3 that when the combination of Picarbutrazox and trifloxystrobin or picoxystrobin controls citrus penicillium in the range of 50:1-1:50, the co-toxicity coefficient is greater than 120, indicating that both are in this range The mixing within is manifested as a gain effect.

Experiment 4: Virulence determination of rice blight

Rice seedlings with consistent growth were selected, and 3 pots of test leaf seedlings were selected for each treatment, sprayed with a potter spray tower at a pressure of 50PSI, about 5mL per pot, and 12 concentration gradients were set for each agent. Bacteria were inoculated 24 hours after the chemical treatment, and conidia were evenly shaken off the leaves of rice blight collected from the field above the rice seedlings for inoculation, and then the rice seedlings were put into the greenhouse for cultivation. After 7 days, the disease index of the whole plant was investigated according to the incidence grading standard of rice blight, and the control effect was calculated, and then the inhibitory intermediate concentration EC50 was calculated by the least square method, and the co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei method.

Table 4: Toxicity test results of the present invention on controlling rice blight

As can be seen from Table 4, when Picarbutrazox and trifloxystrobin or picoxystrobin are in the weight ratio range of 50:1-1:50 for preventing rice blight, the co-toxicity coefficients are all greater than 120, indicating that both are in this range The mixing within is manifested as a gain effect.

Test 5 Toxicity determination of powdery mildew (wheat)

Selected from wheat seedlings with consistent growth, 3 pots of leaf seedlings were selected for each treatment, sprayed with a potter spray tower at a pressure of 50PSI, about 5mL per pot, and 12 concentration gradients were set for each agent. Inoculation was carried out 24 hours after the chemical treatment, and conidia were evenly shaken off the wheat powdery mildew leaves collected from the field above the wheat seedlings for inoculation, and then the wheat seedlings were cultured in the greenhouse. After 7 days, the disease index of the whole plant was investigated according to the incidence classification standard of wheat powdery mildew, and the control effect was calculated, and then the inhibitory intermediate concentration EC50 was calculated by the least square method, and the co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei method.

Table 5: The toxicity test result of the present invention on the prevention and treatment of wheat powdery mildew

As can be seen from Table 5, when Picarbutrazox and trifloxystrobin or picoxystrobin are in the weight ratio range of 50:1-1:50 for the prevention of wheat powdery mildew, the co-toxicity coefficients are all greater than 120, indicating that both are within this range The mixing of all shows a gain effect.

Experiment 6 Toxicity determination of leaf blight (wheat)

Selected from wheat seedlings with consistent growth, 3 pots of leaf seedlings were selected for each treatment, sprayed with a potter spray tower at a pressure of 50PSI, about 5mL per pot, and 12 concentration gradients were set for each agent. Inoculation was carried out 24 hours after the chemical treatment, and the conidia of wheat leaf blight collected from the field were evenly shaken off the conidia on the wheat seedlings for inoculation, and then the wheat seedlings were cultured in the greenhouse. After 7 days, the disease index of the whole plant was investigated according to the incidence grading standard of wheat leaf blight, and the control effect was calculated, and then the inhibitory medium concentration EC50 was calculated by the least square method, and the co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei method.

Table 6: The toxicity test result of the present invention on the prevention and treatment of wheat leaf blight

As can be seen from Table 6, when Picarbutrazox and trifloxystrobin or picoxystrobin are in the weight ratio range of 50:1-1:50 for preventing wheat leaf blight, the co-toxicity coefficients are all greater than 120, indicating that both are in this range The mixing within is manifested as a gain effect.

2. Determination of drug efficacy:

A synergistic effect exists when the effect of the active compound combination exceeds the sum of the effects of the individual active compounds when administered individually. The expected effect of a particular combination of two active compounds can be calculated using the so-called "Colby's formula" (see S.R. Colby, "Calculating Synergistic and Antagonistic Responses of Herbicide Combinations", Weeds 1967, 15, 20-22) as follows: If

X is the activity when using the active compound A in mg/ha or mppm concentration;

Y is the activity when active compound B is used at a rate of ng/ha or at a concentration of nppm, expressed as a percentage of the untreated control;

E is the activity when active compounds A and B are used in amounts m and ng g/ha or concentrations m and n ppm,

So

If the actually observed activity (O) is greater than the expected activity (E), then the composition is synergistic.

The following biological test examples are used to illustrate the present invention. However, the present invention is not limited to these Examples.

Trial 7: Blight Test (Tomato) / Protective Drug Efficacy Test

Solvent: 24.5 parts by weight acetone

24.5 parts by weight dimethylacetamide

Emulsifier: 1 part by weight of alkyl aryl polyglycol ether

To obtain a suitable formulation of the active compound, 1 part by weight of the active compound is mixed with a quantity of solvent and emulsifier, and the concentrate is diluted with water to the desired concentration.

To test for protective activity, young plants are sprayed with the preparation of active compound at a certain application rate. After the sprayed coating has dried on, the plants are inoculated with an aqueous spore suspension of Phytophthora infestans. The plants are then placed in an incubator at approximately 20° C. and a relative atmospheric humidity of 100%.

Three days after incubation, the test results were evaluated. 0% means that the drug effect is equivalent to the control sample, and 100% drug effect means that no symptoms appear.

The above table clearly shows that according to the fungicidal composition of the present invention, the weight ratio of Picarbutrazox to trifloxystrobin or picoxystrobin is in the range of 50:1-1:50, and the actual drug effect on tomato blight is higher than the calculated Drug efficacy, that is, there is an obvious synergistic effect.

Trial 8: Blight Test (Tomato) / Therapeutic Drug Efficacy Test

Solvent: 24.5 parts by weight acetone

24.5 parts by weight dimethylacetamide

Emulsifier: 1 part by weight of alkyl aryl polyglycol ether

To obtain a suitable formulation of the active compound, 1 part by weight of the active compound is mixed with a quantity of solvent and emulsifier, and the concentrate is diluted with water to the desired concentration.

To test for therapeutic activity, tomatoes at the 5-6 leaf stage were inoculated with a spore suspension of Phytophthora infestans. The plants are then placed in an incubator at approximately 20° C. and a relative atmospheric humidity of 100% for 18 hours. After allowing the foliage to dry, the young plants are sprayed with the preparation of active compound at the rate of application so that the liquid drips and are allowed to develop disease in the greenhouse. The degree of disease was investigated 7 days after the inoculation. Evaluate the test results. 0% means that the drug effect is equivalent to the control sample, and 100% drug effect means that no symptoms appear.

The above table clearly shows that according to the fungicidal composition of the present invention, the weight ratio of Picarbutrazox to trifloxystrobin or picoxystrobin is in the range of 50:1-1:50, and the actual drug effect on tomato blight is higher than the calculated Drug efficacy, that is, there is an obvious synergistic effect.

Trial 9: Downy Mildew Test (Grape) / Protective Efficacy Test

Solvent: 24.5 parts by weight acetone

24.5 parts by weight dimethylacetamide

Emulsifier: 1 part by weight of alkyl aryl polyglycol ether

To obtain a suitable formulation of the active compound, 1 part by weight of the active compound is mixed with a quantity of solvent and emulsifier, and the concentrate is diluted with water to the desired concentration.

To test for protective activity, young plants are sprayed with the preparation of active compound at a certain application rate. After the sprayed coating has dried on, the plants are inoculated with an aqueous spore suspension of P. viticola. The plants are then placed in an incubator at approximately 20° C. and a relative atmospheric humidity of 100%.

Three days after incubation, the test results were evaluated. 0% means that the drug effect is equivalent to the control sample, and 100% drug effect means that no symptoms appear.

The above table clearly shows that according to the fungicidal composition of the present invention, the weight ratio of Picarbutrazox to trifloxystrobin or picoxystrobin is within the scope of 50:1-1:50, and the actual drug effect on grape downy mildew is higher than the calculation The obtained drug effect, that is, there is a synergistic effect, and the effect of the synergistic effect is the most obvious.

Trial 10: Downy Mildew Test (Grape) / Therapeutic Drug Efficacy Test

Solvent: 24.5 parts by weight acetone

24.5 parts by weight dimethylacetamide

Emulsifier: 1 part by weight of alkyl aryl polyglycol ether

To obtain a suitable formulation of the active compound, 1 part by weight of the active compound is mixed with a quantity of solvent and emulsifier, and the concentrate is diluted with water to the desired concentration.

To test the curative activity, grapes at the 5-6 leaf stage in nursery pots were inoculated with a spore suspension of grape downy mildew and placed in an incubator at 25° C. and a relative atmospheric humidity of 100% for 18 hours. After the leaves have been allowed to air-dry, the plants are sprayed with the preparation of active compound at the rate of application and then allowed to disease in the greenhouse. The degree of disease was investigated 10 days after the inoculation. 0% means that the drug effect is equivalent to the control sample, and 100% drug effect means that no symptoms appear.

The above table clearly shows that according to the fungicidal composition of the present invention, the mass ratio of Picarbutrazox to trifloxystrobin or picoxystrobin is within the scope of 50:1-1:50, and the actual drug effect on grape downy mildew is higher than the calculation The obtained drug effect, that is, there is a synergistic effect, and the effect of the synergistic effect is the most obvious.

Experiment 11: Preservative effect on stored citrus

Treat citrus fruits during the harvest period, remove diseased and injured fruits, and soak the fruits with the treatment liquid for 1 min. Compared with water immersion. The fruits of each treatment were soaked and dried in the air, put into a plastic box with newspapers around it the next day, and stored indoors at room temperature. After 7 days of treatment, the number of rotten fruits was checked and rotten fruits were removed, and the weight of good fruits was weighed at the same time. And calculate the prevention effect.

The above table clearly shows that according to the fungicidal composition of the present invention, the weight ratio of Picarbutrazox to trifloxystrobin or picoxystrobin is in the range of 50:1-1:50, and the actual drug effect on the preservation of citrus during the storage period is higher than that of The calculated efficacy means that there is an obvious synergistic effect.

Trial 12: Blight test (rice) / therapeutic efficacy test

Solvent: 24.5 parts by weight acetone

24.5 parts by weight dimethylacetamide

Emulsifier: 1 part by weight of alkyl aryl polyglycol ether

To obtain a suitable formulation of the active compound, 1 part by weight of the active compound is mixed with a quantity of solvent and emulsifier, and the concentrate is diluted with water to the desired concentration.

In order to test the therapeutic activity, rice seedlings at the 5-6 leaf stage in nursery pots were inoculated with an aqueous spore suspension of R. solani fungus and placed in an incubator at 25° C. and a relative atmospheric humidity of 100% for 18 hours. After the leaves have been allowed to air-dry, the plants are sprayed with the preparation of active compound at the rate of application and then allowed to disease in the greenhouse. The degree of disease was investigated 10 days after the inoculation. 0% means that the drug effect is equivalent to the control sample, and 100% drug effect means that no symptoms appear.

The above table clearly shows that according to the fungicidal composition of the present invention, the weight ratio of Picarbutrazox to trifloxystrobin or picoxystrobin is in the range of 50:1-1:50, and the actual drug effect on rice blight is higher than the calculation The obtained drug effect, that is, there is a synergistic effect, and the effect of the synergistic effect is the most obvious.

Trial 13: Powdery Mildew Test (Wheat) / Therapeutic Efficacy Test

Solvent: 24.5 parts by weight acetone

24.5 parts by weight dimethylacetamide

Emulsifier: 1 part by weight of alkyl aryl polyglycol ether

To obtain a suitable formulation of the active compound, 1 part by weight of the active compound is mixed with a quantity of solvent and emulsifier, and the concentrate is diluted with water to the desired concentration.

To test the therapeutic activity, wheat seedlings at the 5-6 leaf stage in nursery pots were inoculated with an aqueous spore suspension of powdery mildew of wheat and placed in an incubator at 25° C. and a relative atmospheric humidity of 100% for 18 hours. After allowing the leaves to dry, the plants are sprayed with the preparation of active compound at the rate of application and then allowed to disease in the greenhouse. The degree of disease was investigated 10 days after the inoculation. 0% means that the drug effect is equivalent to the control sample, and 100% drug effect means that no symptoms appear.

The above table clearly shows that according to the fungicidal composition of the present invention, the weight ratio of Picarbutrazox to trifloxystrobin or picoxystrobin is in the range of 50:1-1:50, and the actual drug effect on wheat powdery mildew is higher than the calculated The drug effect, that is, there is a synergistic effect, and the effect of the synergistic effect is the most obvious.

Claims (12)

1. A bactericidal composition, characterized in that: said bactericidal composition comprises active ingredient Picarbutrazox and trifloxystrobin or picoxystrobin, and the weight ratio of Picarbutrazox and trifloxystrobin or picoxystrobin is 50:1-1 :50, preferably 25:1-1:25, more preferably 10:1-1:10, more preferably 5:1-1:5. 2. The fungicidal composition according to claim 1, characterized in that: the sum of the Picarbutrazox and trifloxystrobin or picoxystrobin mass accounts for 5%-90% of the mass of the fungicidal composition, more preferably 10% -80%, more preferably 20%-60%. 3. The bactericidal composition according to claim 1, characterized in that: the dosage form of the bactericidal composition is a suspension, a seed coating, a wettable powder, a water dispersible granule, a microcapsule suspension, a coated granule , Extruded granules, emulsifiable concentrates, microemulsions, water emulsions, effervescent tablets, ultra-low volume liquids, suspoemulsions. 4. The fungicidal composition according to claim 1, further comprising a filler and/or a surfactant. 5. A method for preventing and treating plant pathogenic bacteria, characterized in that, the bactericidal composition according to claim 1 acts on pathogenic bacteria and/or its environment, or in plants, seeds, soil, regions, materials or spaces . 6 . The method according to claim 5 , wherein the Picarbutrazox and trifloxystrobin or picoxystrobin according to claim 1 are administered simultaneously, separately, or sequentially. 7. The fungicidal composition of claim 1 is used for controlling fungi and bacteria. 8. The fungicidal composition as claimed in claim 1 is used to control fungi and bacteria on cereals, fruits and vegetables. 9. Use of the fungicidal composition according to claim 1 for protecting plant propagation material and subsequently grown plant organs. 10. The application of the fungicidal composition according to claim 1 to the places where control is required to prevent and control pathogenic or saprophytic fungi and bacteria in soil or cultivation media. 11. The use of the fungicidal composition according to claim 1 for preventing and treating diseases during storage of fruits and vegetables. 12. A method for controlling phytopathogenic fungi of plants, plant parts, plant propagation materials and plant organs that grow out subsequently, the method comprising applying the fungicidal composition of claim 1 in an agronomically effective and substantially non-phytotoxic application Application by seed treatment, foliar application, stem application, drenching, dripping, pouring, spraying, spraying, dusting, spreading or fuming to plants, plant parts, plant propagation material or in which plants are growing or need to be applied growing soil or growing medium.