CN106978340B - A kind of Bifidobacterium culture apparatus special - Google Patents

Abstract

The invention discloses a special cultivation device for bifidobacteria, which comprises a base, a support frame, a box body, a constant temperature device and an illumination device. A support frame is arranged on the top of the base, and the box body is arranged on the support frame. The box body includes a freeze-drying chamber , a cultivation room and a testing room, the inside of the training room is provided with a constant temperature device and an illumination device, the constant temperature device is arranged on the wall of the cultivation room, the lighting device is provided on the top of the cultivation room, the detection room and the cultivation room are separated by a first partition, and there are The detection device, the freeze-drying chamber and the cultivation chamber are connected through the second partition, and a freeze-drying device is installed inside; a sleeve is provided on one side of the cultivation chamber, and a sealing ring is provided between the sleeve and the box, and the bottom of the cultivation chamber is installed. There is a cultivation plate, and a thermometer and a hygrometer are arranged in the middle, and both the thermometer and the hygrometer are installed on the inner wall of the box; the present invention is simple in structure, safe and convenient to use, and can effectively provide a good cultivation space for bifidobacteria, and detect The cultivation efficiency and viability of bifidobacteria are improved, and at the same time, it is convenient for cultivators to perform real-time operations.

Description

A special cultivation device for bifidobacteria

technical field

The invention relates to the technical field of strain cultivation, in particular to a special cultivation device for bifidobacteria.

Background technique

Bifidobacterium is an anaerobic Gram-positive bacillus with often forked ends, hence the name Bifidobacterium. The number of bifidobacteria distributed in the gastrointestinal tract decreased with age, and the most distributed in breast-fed infants. It has been found that there are 32 subtypes of Bifidobacteria, and there are as many as 70 biological agents containing Bifidobacteria. Bifidobacteria in infants account for 60% of the total intestinal bacteria, while those over 60 years old only account for 7.9%; Bifidobacteria can treat chronic diarrhea. The clinical observation and research on chronic diarrhea patients with bifidobacterium showed that after taking the medicine for two weeks, the frequency and shape of the stool were abnormal, and the clinical symptoms disappeared. The total effective rate was 90.3%, and the recurrence rate was low. Many domestic hospitals have used bifidobacterium preparations as the first choice drug for the treatment of chronic diarrhea. Antibiotic-associated enteritis is actually the use of antibiotics, which makes the original passing bacteria or foreign bacteria (such as Enterobacteriaceae) the dominant population. They proliferate in large quantities or secrete related toxins that bind to the receptors of intestinal mucosal epithelial cells to increase the activity of CAMP enzymes. A large amount of water, salt and electrolytes are lost, resulting in diarrhea symptoms

Bifidobacterium is one of the beneficial bacteria in the human intestinal tract, which plays an important role in human health, such as treating constipation, preventing tumors, protecting the liver, etc., and is now being used in more and more food industries. However, bifidobacteria have higher requirements on its cultivation environment, so a convenient, safe and convenient cultivation device is needed.

Contents of the invention

In order to solve the above existing problems, the present invention provides a special cultivation device for bifidobacteria.

The present invention is realized through the following technical solutions:

A special cultivation device for bifidobacteria, comprising a base, a support frame, a box body, a constant temperature device and a lighting device, the inside of the base is provided with a power storage device, and the power storage device is a storage battery, and the bottom of the base is provided with rollers; the base A support frame is arranged on the top, and the box body is arranged on the support frame. The box body includes a freeze-drying chamber, a cultivation chamber and a detection chamber, and a constant temperature device and an illumination device are arranged inside the cultivation chamber. On the inner wall, the lighting device is provided on the top of the box, a sleeve is provided on one side of the cultivation chamber, and a sealing ring is provided between the sleeve and the cabinet, and a cultivation tray is provided at the bottom of the cultivation chamber. A thermometer and a hygrometer are also arranged inside the cultivation chamber, and both the thermometer and the hygrometer are arranged on the inner wall of the box; the cultivation chamber and the detection chamber are connected through a first partition, and a sliding door is arranged on the first partition, so The top of the detection chamber is provided with a sealing cover, and the sealing cover is provided with a lock, the top of the cultivation chamber is provided with a vacuum pump, and the vacuum pump is connected to the cultivation chamber through a conduit, the bottom of the cultivation chamber is provided with a turntable, and the top of the turntable is set There is a cultivation tray; the other side of the cultivation chamber is connected with the freeze-drying chamber through a second partition, and a sliding door is arranged on the second partition.

Preferably, a detection device and a bracket are also provided inside the detection chamber, the bracket is arranged at the bottom of the detection chamber, and the detection device is arranged on the top of the bracket, and the detection device includes a backing plate, a clock, an aspirator, vibrator and chassis, the bottom of the backing plate is set on the top of the bracket, the top of the backing plate is set with a vibrator, the top of the vibrator is set with a chassis, the clock is set on the front side of the bracket, and the attractor passes through The hook is connected with the inner wall of the detection chamber, a detection tube is arranged inside the chassis, and a detection liquid is arranged inside the detection tube.

Preferably, a nitrogen port is provided on the top of the freeze-drying chamber, and a nitrogen valve is provided on the nitrogen port, a freeze-dried eggplant bottle is arranged inside the freeze-dried chamber, and the freeze-dried eggplant bottle is arranged on the base, and the freeze-dried eggplant bottle is arranged on the base. There are several dried eggplant bottles.

Preferably, the constant temperature device includes a heating rod, a connecting frame, a sensor and a humidifier, one end of the connecting frame is arranged on the inner wall of the cultivation chamber, the other end of the connecting frame is provided with a heating rod and a humidifier, and the sensor is arranged on on the connection rack. Preferably, the lighting device includes an ultraviolet lamp and an incandescent lamp, the ultraviolet lamp is arranged on the top of the cultivation chamber, and the incandescent lamp is arranged on the inner top of the sealing cover.

Preferably, the first partition includes a front partition and a rear partition, a sliding door is provided between the front partition and the rear partition, a handle is provided on the sliding door, and a seal is provided around the sliding door. A chute is provided on the front side of the front partition, and a buckle is provided on the chute.

Preferably, its use method comprises the following steps:

A. Put the original flora into the cultivation tray, then use the vacuum pump to vacuum the cultivation chamber, and use the ultraviolet lamp to sterilize in the box; the pump pressure of the vacuum pump is 10-25MBAR; the sterilization time is 10min-30min;

B. After that, use the heating rod and humidifier to precisely control the temperature and humidity in the box to achieve an ideal cultivation environment. At the same time, the cultivation personnel can observe in real time and perform internal operations through the sleeve; the temperature is 30°C-40°C; the humidity is 20%-50%;

C. After the cultivation is completed, through the sliding door on the first partition, the bifidobacteria in the cultivation plate will pass through the aspirator; drawn into the detection tube; wherein, the aspirator adopts a vacuum extrusion aspirator, and the aspirator is sucked by extrusion. The extrusion head at the upper end of the device sucks the bifidobacteria into the suction tube, and then extrudes it into the detection tube; at the same time, a nutrient agar medium is set in the detection tube;

D. After waiting 36-48 hours in the nutrient agar medium, check the culture effect of bifidobacteria;

E, open the second clapboard afterwards, put the well-cultivated bifidobacteria into the freeze-drying eggplant bottle inside the freeze-drying chamber, and then open the nitrogen valve on the top of the freeze-drying chamber to perform quick freeze-drying.

Compared with the existing technology, the beneficial effects of the present invention are: the present invention is simple in structure, safe and convenient to use, can effectively provide a good cultivation space for bifidobacteria, and improve the cultivation efficiency and viability of bifidobacteria, and at the same time It is convenient for cultivators to carry out real-time operation; in order to have better battery life and to deal with the occurrence of special situations, the present invention adopts a storage battery inside the base; in order to be able to better cultivate and take it, the present invention adopts a There are double compartments, and sliding doors are set on the partitions. In order to make it more convenient for the cultivators to operate from time to time, and at the same time, they cannot be directly touched. A layer of elastic rare earth protective layer is sprayed on the top. In order to better cultivate high-quality bifidobacteria, the present invention adopts a freeze-drying chamber on the left side of the cultivation chamber and a detection device on the right side.

Description of drawings

Fig. 1 is a schematic diagram of the overall structure of the present invention;

Fig. 2 is a schematic diagram of a partial body structure of the present invention;

Fig. 3 is a schematic diagram of the front view of the first partition of the present invention;

Fig. 4 is a schematic diagram of the side view structure of the first separator of the present invention;

Fig. 5 is an enlarged schematic view of the turntable structure of the present invention.

detailed description

Below in conjunction with accompanying drawing and specific embodiment the present invention is described in further detail:

As shown in Figure 1-5, a special culture device for bifidobacteria, including a base 1, a support frame 2, a box body 3, a constant temperature device 4 and a lighting device 5, the top of the base 1 is used as a support frame 2, and the box body 3 is set On the support frame 2, the box body 3 includes a freeze-drying chamber 38, a cultivation chamber 10 and a detection chamber 11, and the interior of the cultivation chamber 10 is provided with a constant temperature device 4 and an illumination device 5. 5. The top of the cultivation chamber 10 is set, a sleeve 6 is arranged on one side of the cultivation chamber, and a sealing ring is arranged between the sleeve 6 and the cultivation chamber 10, a cultivation tray 7 is arranged at the bottom of the middle part of the cultivation chamber 10, and a cultivation tray 7 is arranged on the bottom of the cultivation chamber 10. A thermometer 8 and a hygrometer 9 are also arranged inside, and both the thermometer 8 and the hygrometer 9 are arranged on the inner wall of the box; the bottom of the base 1 is provided with a roller 23 . The invention has the advantages of simple structure, safe and convenient use, can effectively provide a good cultivation space for bifidobacteria, improve the cultivation efficiency and survival degree of bifidobacteria, and is convenient for cultivators to operate from time to time.

In the present invention, the cultivation chamber 10 and the detection chamber 11 are connected by the first partition 12, the first partition 12 is provided with a sliding door, the top of the detection chamber 11 is provided with a sealing cover 13, and the sealing cover 13 is provided with a lock The top of the cultivation chamber 10 is provided with a vacuum pump 14, and the vacuum pump 14 is connected to the cultivation chamber 10 through a conduit, the bottom of the cultivation chamber 10 is provided with a turntable 15, and the top of the turntable 15 is provided with a cultivation disk 7. The thermostatic device 4 comprises a heating rod 16, a connecting frame 17, a sensor 18 and a humidifier 19, one end of the connecting frame 17 is arranged on the inner wall of the cultivation chamber 10, the other end of the connecting frame 17 is provided with a heating rod 16 and a humidifier 19, and the sensor 18 is arranged on On the connecting frame 17; the partition 12 includes a front partition 32 and a rear partition 33, a sliding door 34 is arranged between the front partition 32 and the rear partition 33, a handle is provided on the sliding door 34, and a seal is provided around the sliding door 34. Parts, a chute 35 is provided on the front side of the front partition 32, and a buckle 36 is provided on the chute 35; a detection device 24 and a bracket 25 are also provided inside the detection chamber 11, and the bracket 25 is arranged at the bottom of the detection chamber 22 , the detection device 24 is arranged on the top of the bracket 25, the detection device 24 includes a backing plate 26, a clock 27, an attractor 28, a vibrator 29 and a chassis 30, the bottom of the backing plate 26 is arranged on the top of the bracket 25, and the top of the backing plate 26 is provided with A vibrator 29, a chassis 30 is arranged on the top of the vibrator 29, a clock 27 is arranged on the front side of the bracket 25, an aspirator 28 is connected to the inner wall of the detection chamber 11 through a hook, a detection tube 31 is arranged inside the chassis 30, and a detection tube 31 is arranged inside There is a detection solution; the other side of the cultivation chamber 10 is connected with the freeze-drying chamber 38 through the second partition 37, the second partition 37 is provided with a sliding door, and the top of the freeze-drying chamber 38 is provided with a nitrogen port 39, and the nitrogen port 39 is provided with a nitrogen valve 40, the inside of the freeze-drying chamber 38 is provided with freeze-dried eggplant bottles 41, and the freeze-dried eggplant bottles 41 are arranged on the base 42, and there are several freeze-dried eggplant bottles 41; the present invention is To be able to better cultivate high-quality bifidobacteria, a detection device is installed inside the detection chamber; in the present invention, in order to better cultivate and take it, a double compartment is provided inside the box, and A sliding door is arranged on the first partition. The lighting device 5 includes an ultraviolet lamp 20 and an incandescent lamp 21 , the ultraviolet lamp 20 is arranged on the top of the cultivation chamber 10 , and the incandescent lamp 21 is arranged on the inner top of the sealing cover plate 13 . The sleeve 6 is provided with a rare earth protective layer. In the present invention, in order to be more convenient for cultivators to operate from time to time, and at the same time not be able to touch directly, a sleeve is provided on the box, and a layer of elastic rare earth protective layer is sprayed on the sleeve; There is an electric storage device 22, and the electric storage device 22 is a storage battery; it is better for subsequent escort.

Embodiment one:

The usage method of this embodiment includes the following steps:

A. Put the original flora into the cultivation tray, then use the vacuum pump to vacuum the cultivation chamber, and use the ultraviolet lamp to sterilize in the box; the pump pressure of the vacuum pump is 10MBAR; the sterilization time is 10min;

B. Then use the heating rod and humidifier to precisely control the temperature and humidity in the box to achieve an ideal cultivation environment. At the same time, the cultivation personnel can observe in real time and perform internal operations through the sleeve; the temperature is 30°C; the humidity is 20%;

C. After the cultivation is completed, through the sliding door on the first partition, the bifidobacteria in the cultivation plate will pass through the aspirator; drawn into the detection tube; wherein, the aspirator adopts a vacuum extrusion aspirator, and the aspirator is sucked by extrusion. The extrusion head at the upper end of the device sucks the bifidobacteria into the suction tube, and then extrudes it into the detection tube; at the same time, a nutrient agar medium is set in the detection tube;

D. After waiting for 36 hours in the nutrient agar medium, check the culture effect of bifidobacteria;

E, open the second clapboard afterwards, put the well-cultivated bifidobacteria into the freeze-drying eggplant bottle inside the freeze-drying chamber, and then open the nitrogen valve on the top of the freeze-drying chamber to perform quick freeze-drying.

Embodiment two:

The usage method of this embodiment includes the following steps:

A. Put the original flora into the cultivation tray, and then use the vacuum pump to vacuum the cultivation chamber, and use the ultraviolet lamp to sterilize in the box; the pump pressure of the vacuum pump is 25MBAR; the sterilization time is 30min;

B. After that, use the heating rod and humidifier to precisely control the temperature and humidity in the box to achieve an ideal cultivation environment. At the same time, the cultivation personnel can observe in real time and perform internal operations through the sleeve; the temperature is 40°C; the humidity is 50%;

C. After the cultivation is completed, through the sliding door on the first partition, the bifidobacteria in the cultivation plate will pass through the aspirator; drawn into the detection tube; wherein, the aspirator adopts a vacuum extrusion aspirator, and the aspirator is sucked by extrusion. The extrusion head at the upper end of the device sucks the bifidobacteria into the suction tube, and then extrudes it into the detection tube; at the same time, a nutrient agar medium is set in the detection tube;

D. After waiting for 48 hours on the nutrient agar medium, check the culture effect of bifidobacteria;

E, open the second clapboard afterwards, put the well-cultivated bifidobacteria into the freeze-drying eggplant bottle inside the freeze-drying chamber, and then open the nitrogen valve on the top of the freeze-drying chamber to perform quick freeze-drying.

Embodiment three:

The usage method of this embodiment includes the following steps:

A. Put the original flora into the cultivation tray, then use the vacuum pump to vacuum the cultivation chamber, and use the ultraviolet lamp to sterilize in the box; the pump pressure of the vacuum pump is 20MBAR; the sterilization time is 20min;

B. Afterwards, use the heating rod and humidifier to precisely control the temperature and humidity in the box to achieve an ideal cultivation environment. At the same time, the cultivation personnel can observe in real time and perform internal operations through the sleeve; the temperature is 35°C; the humidity is 35%;

C. After the cultivation is completed, through the sliding door on the first partition, the bifidobacteria in the cultivation plate will pass through the aspirator; drawn into the detection tube; wherein, the aspirator adopts a vacuum extrusion aspirator, and the aspirator is sucked by extrusion. The extrusion head at the upper end of the device sucks the bifidobacteria into the suction tube, and then extrudes it into the detection tube; at the same time, a nutrient agar medium is set in the detection tube;

D. After waiting for 42 hours in the nutrient agar medium, check the culture effect of bifidobacteria;

E, open the second clapboard afterwards, put the well-cultivated bifidobacteria into the freeze-drying eggplant bottle inside the freeze-drying chamber, and then open the nitrogen valve on the top of the freeze-drying chamber to perform quick freeze-drying.

The basic principles, main features and advantages of the present invention have been shown and described above. Those skilled in the industry should understand that the present invention is not limited by the above-mentioned embodiments. What are described in the above-mentioned embodiments and the description only illustrate the principle of the present invention. Without departing from the spirit and scope of the present invention, the present invention will also have Variations and improvements are possible, which fall within the scope of the claimed invention. The protection scope of the present invention is defined by the appended claims and their equivalents.

Claims (6)

1. A method for using a special culture device for bifidobacteria, characterized in that: the special culture device for bifidobacteria includes a base (1), a support frame (2), a casing (3), and a constant temperature device (4) and the lighting device (5), the base (1) is provided with an electric storage device (22), and the electric storage device (22) is a storage battery, and the bottom of the base (1) is provided with a roller (23); the base (1) ) top is provided with support frame (2), and described casing (3) is arranged on the support frame (2), and described casing (3) comprises lyophilization chamber (38), cultivation chamber (10) and detection chamber (11 ), the inside of the cultivation chamber (10) is provided with a constant temperature device (4) and a lighting device (5), the constant temperature device (4) is arranged on the inner wall of the cultivation chamber (10), and the lighting device (5) is set to cultivate The top of the chamber (10), a sleeve (6) is arranged on one side of the cultivation chamber (10), and a sealing ring is arranged between the sleeve (6) and the cultivation chamber (10), and the cultivation chamber (10 ) is provided with a cultivation tray (7) at the inner bottom, and a thermometer (8) and a hygrometer (9) are also provided inside the cultivation chamber (10), and the thermometer (8) and the hygrometer (9) are all arranged on the inner wall of the box. The cultivation chamber (10) and the detection chamber (11) are connected by the first partition (12), the first partition (12) is provided with a sliding door, and the top of the detection chamber (11) is provided with a sealing Cover plate (13), and the sealing cover plate (13) is provided with a lock, the top of the cultivation chamber (10) is provided with a vacuum pump (14), and the vacuum pump (14) is connected with the cultivation chamber (10) through a conduit, the The bottom of the cultivation chamber (10) is provided with a turntable (15), and the top of the turntable (15) is provided with a cultivation tray (7); the other side of the cultivation chamber (10) is connected to the freeze-drying chamber ( 38) connection, the second partition (37) is provided with a sliding door; The using method of described bifidobacteria special culture device comprises the following steps: A. Put the original flora into the cultivation tray, and then use the vacuum pump to vacuum the cultivation chamber, and use the ultraviolet lamp to sterilize in the box; the pump pressure of the vacuum pump is 10-25MBAR; the sterilization time is 10min-30min; B. After that, use the heating rod and humidifier to precisely control the temperature and humidity in the box to achieve an ideal cultivation environment. At the same time, the cultivation personnel can observe in real time and perform internal operations through the sleeve; the temperature is 30°C-40°C; the humidity is 20%-50%; C. After the cultivation is completed, through the sliding door on the first partition, the bifidobacteria in the cultivation plate will be sucked into the detection tube through the aspirator; wherein, the aspirator adopts a vacuum extrusion aspirator, and through the extrusion aspirator The extrusion head at the upper end sucks the bifidobacteria into the suction tube, and then extrudes it into the detection tube; at the same time, a nutrient agar medium is set in the detection tube; D. After waiting 36-48 hours in the nutrient agar medium, check the culture effect of bifidobacteria; E, open the second clapboard afterwards, put the well-cultivated bifidobacteria into the freeze-dried eggplant bottle inside the freeze-drying chamber, and then open the nitrogen valve on the top of the freeze-drying chamber for quick freeze-drying. 2. The method according to claim 1, characterized in that: the inside of the detection chamber (11) is also provided with a detection device (24) and a bracket (25), and the bracket (25) is arranged in the detection chamber ( 11) Inner bottom, the detection device (24) is arranged on the top of the bracket (25), and the detection device (24) includes a backing plate (26), a clock (27), an attractor (28), a vibrator (29) ) and chassis (30), the bottom of the backing plate (26) is arranged on the top of the bracket (25), the top of the backing plate (26) is provided with a vibrator (29), and the top of the backing plate (29) is provided with Chassis (30), the clock (27) is arranged on the front side of the bracket (25), the suction device (28) is connected to the inner wall of the detection chamber (11) through a hook, and a detection tube is arranged inside the chassis (30) (31), and the inside of the detection tube (31) is provided with a detection liquid. 3. The method according to claim 1, characterized in that: the top of the freeze-drying chamber (38) is provided with a nitrogen port (39), and the nitrogen port (39) is provided with a nitrogen valve (40), the freezer Freeze-dried eggplant bottles (41) are arranged inside the drying chamber (38), and the freeze-dried eggplant bottles (41) are arranged on the base (42), and there are several freeze-dried eggplant bottles (41). 4. The method according to claim 1, characterized in that: the thermostatic device (4) comprises a heating rod (16), a connecting frame (17), a sensor (18) and a humidifier (19), and the connecting frame (17) one end is arranged on the inner wall of the cultivation chamber (10), the other end of the connecting frame (17) is provided with a heating rod (16) and a humidifier (19), and the sensor (18) is arranged on the connecting frame (17) superior. 5. The method according to claim 1, characterized in that: the lighting device (5) comprises an ultraviolet lamp (20) and an incandescent lamp (21), and the ultraviolet lamp (20) is arranged on the top of the cultivation chamber (10) , the incandescent lamp (21) is arranged on the inner top of the sealing cover plate (13). 6. The method according to claim 1, characterized in that: the first partition (12) comprises a front partition (32) and a rear partition (33), and the front partition (32) and the rear partition (33) There is a sliding door (34) between them, a handle is provided on the sliding door (34), a seal is provided around the sliding door (34), and a front side of the front partition (32) is provided with A chute (35), and a buckle (36) is arranged on the chute (35).