CN201429599Y - Quantitative and rapid detection of tetracycline up-transfer phosphorescence detection test strip - Google Patents

Abstract

The utility model discloses an up-converting phosphor detection test paper strip capable of checking achromycin quantitatively and rapidly. The test paper strip comprises four zones of a sample absorbing zone, a marker zone, a result scanning window and an end point indicating window, wherein the up-converting phosphor detection test paper strip capable of checking achromycin quantitatively and rapidly is divided into a bottom board, glass fiber paper, an immunity chromatography composite film, a marker and water absorbing paper. The test paper strip is characterized in that the marker is up-converting phosphor particles, and has the advantages of quantitative detection, excellent sensitivity, stability and safety, simple operation, and automatic result judgment and reading.

Description

Quantitative and rapid detection of tetracycline up-transfer phosphorescence detection test strip

technical field

The utility model belongs to the detection technology of immunochromatography, specifically, a test strip for rapid and quantitative detection of tetracycline by means of an immunochromatographic reaction of up-conversion phosphorescence technology (UPT).

Background technique

One of the common problems in the livestock and poultry farming industry is antibiotic residues, among which tetracycline is a relatively common residual antibiotic. It plays an important role in the treatment of Gram-positive and negative bacteria, rickettsia, spirochetes and some protozoan infectious diseases, but its residues have relatively large toxic and side effects; the "Veterinary Drugs in Animal Food" revised in 2002 The "Maximum Residue Limits" stipulates corresponding regulations on tetracycline residues in cattle and goat milk, animal muscle, liver, eggs and other products.

In the prior art, the tetracycline residue detection method is mainly high-performance liquid chromatography, but this method requires expensive chromatographic analyzers, professional operating methods, etc. Although the detection is accurate, the cycle is long and cannot meet the needs of rapid detection.

Contents of the invention

The utility model provides an up-transfer phosphorescence detection test strip for quantitative detection of tetracycline.

The technical scheme of the utility model is as follows: an up-transfer phosphorescence detection test strip for quantitative and rapid detection of tetracycline, including four regions: sample absorption area, marker area, result scanning window, and end point indication window. The quantitative and rapid detection of tetracycline The up-transfer phosphorescence detection test strip is divided into bottom plate, glass fiber paper, immunochromatographic composite membrane, marker and absorbent paper, and is characterized in that the marker is up-transfer phosphorescent particle (UCP).

On the basis of immunochromatography test paper, UCP particles are used to mark tetracycline monoclonal antibody. The test paper is composed of UCP markers, sample pads, immunochromatographic composite membranes, absorbent paper, adhesive backing, and test strip shell. The experimental results are provided by UPT. Analytical interpretation of biosensors.

The UPT biosensor is used to detect the phosphorescence signals on the detection band and the control band on the immunochromatography test strips marked with infrared up-conversion fluorescent particles (UCP). It is an optical biosensor based on up-conversion phosphorescence technology (UPT). Sensor; UCP will emit visible light with a wavelength much shorter than the excitation light under the excitation of infrared light; according to the intensity of the visible light, the UPT biosensor can perform rapid quantitative detection of the target object.

Compared with the prior art, the utility model has outstanding advantages and practicability:

1. Fast detection: The detection time is only 15 minutes, which can meet the needs of on-site detection.

2. High detection accuracy and strong specificity: Due to the particularity of up-conversion phosphorescence technology, its luminescence is not affected by the external environment, ensuring the stability of detection to the greatest extent.

3. More convenient and rapid quantitative detection of tetracycline in samples.

4. The result is automatically interpreted by the machine, reducing human error.

Description of drawings

The accompanying drawing shows the appearance of the up-transfer phosphorescence detection test strip for quantitative and rapid detection of tetracycline;

The serial numbers of the above drawings are: 1. Sample injection hole, 2. Result scanning window, 3. End point indicating window, 4. UCP marker.

Detailed ways

Below in conjunction with accompanying drawing embodiment the utility model is described in further detail:

1. Processing of samples to be tested: Mix 90 μl of finished test strip sample diluent and 10 μl of samples to be tested evenly;

2. Adding samples: add 100 μl of the diluted sample to be tested into the sample hole 1 of the test strip, and react with the UCP marker 4;

3. Scanning: After 10 minutes, observe the end point indicator window 3 to confirm the end of the reaction, scan the window 2 with the scanning result of the UPT biosensor, and obtain the corresponding detection result.

Claims (1)

1. An up-conversion phosphorescence detection test strip for quantitative and rapid detection of tetracycline, including four areas: sample absorption area, marker area, result scanning window, and end point indication window. The up-conversion phosphorescence detection test for quantitative and rapid detection of tetracycline The paper strips are divided into bottom plate, glass fiber paper, immunochromatographic composite membrane, marker and water-absorbing paper, and the feature is that the marker is tetracycline monoclonal antibody labeled with phosphorescent particles.

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