DK2479289T3 - Fremgangsmåde til methyleringsanalyse - Google Patents
Fremgangsmåde til methyleringsanalyse Download PDFInfo
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- DK2479289T3 DK2479289T3 DK12164321.7T DK12164321T DK2479289T3 DK 2479289 T3 DK2479289 T3 DK 2479289T3 DK 12164321 T DK12164321 T DK 12164321T DK 2479289 T3 DK2479289 T3 DK 2479289T3
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- dna
- oligonucleotide
- methylation
- septin
- seq
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Claims (6)
1. Fremgangsmåde til methyleringsanalyse, hvilken fremgangsmåde omfatter a) behandling af genomisk DNA med et eller flere reagenser for at omdanne ikke-methylerede cytosinbaser til uracilsulfonat eller til en anden base med en anden bindingsadfærd end cytosin, mens methyleret cytosin forbliver uændret; b) amplifikation af det behandlede DNA ved hjælp af i) et oligonukleotid, der omfatter eller består af en sekvens defineret ifølge SEQ ID NO: 5 eller en variant deraf med 5’-terminal og/eller 3'-terminal deletion af 1, 2, 3, 4 eller 5 nukleotider; og ii) et oligonukleotid, der omfatter eller består af en sekvens som defineret ifølge SEQ ID NO: 44 eller en variant deraf med 5’-terminal og/eller 3’- terminal deletion af 1, 2, 3, 4 eller 5 nukleotider; hvor oligonukleotideme er egnede til anvendelse som primere; c) udledning af tilstedeværelsen eller fraværet af methylering af CpG-dinukleotideme, der er amplificeret i trin b), fra resultaterne fra trin b).
2. Fremgangsmåde ifølge krav 1, hvor amplifikationen af fremgangsmådetrin b) endvidere omfatter anvendelse af i) mindst ét oligonukleotid, der i alt væsentligt omfatter eller består af en sekvens udvalgt fra gruppen bestående af SEQ ID NO: 7 og 49-57, hvor det ene eller flere oligonukleotider er egnede til anvendelse som blokkere; ii) mindst ét oligonukleotid, der i alt væsentligt omfatter eller består af en sekvens udvalgt fra gruppen bestående af SEQ ID NO: 8 og 58-61, hvor det ene eller flere oligonukleotider er egnede til anvendelse som prober; og iii) en polymerase, fortrinsvis en varmestabil polymerase.
3. Fremgangsmåde ifølge krav 2, hvilken fremgangsmåde omfatter a) anvendelse af et oligonukleotid, der i alt væsentligt omfatter eller består af sekvensen ifølge SEQ ID NO: 7 i punkt (i); og b) anvendelse af et oligonukleotid, der i alt væsentligt omfatter eller består af sekvensen ifølge SEQ ID NO: 8 i punkt (ii).
4. Fremgangsmåde til detektering og/eller klassificering af cellulære proliferative forstyrrelser, hvilken fremgangsmåde omfatter: a) behandling af genomisk DNA med et eller flere reagenser for at omdanne ikke-methylerede cytosinbaser til uracilsulfonat eller til en anden base med en anden bindingsadfærd end cytosin, mens methyleret cytosin forbliver uændret; b) amplification af det behandlede DNA ved hjælp af i) et oligonukleotid, der omfatter eller består af en sekvens som defineret ifølge SEQ ID NO: 5 eller varianter deraf med 5’-terminal og/eller 3'-terminal deletion af 1, 2, 3, 4 eller 5 nukleotider, hvor det ene eller flere oligonukleotider er egnede til anvendelse som primere; og ii) et oligonukleotid, der omfatter eller består af en sekvens som defineret ifølge SEQ ID NO: 44 eller varianter deraf med 5'-terminal og/eller 3’-terminal deletion af 1, 2, 3, 4 eller 5 nukleotider; hvor det ene eller flere oligonukleotider er egnede til anvendelse som primere; c) udledning af tilstedeværelsen eller fraværet af methylering af CpG-dinukleotideme, der er amplificeret i trin b), fra resultatet fra trin b), hvorved mindst én af detektering og klassificering af cellulære proliferative forstyrrelser, mindst delvist, muliggøres.
5. Fremgangsmåde ifølge krav 4, hvor amplifikationen af fremgangsmådetrinnet b) endvidere omfatter anvendelsen af i) mindst ét oligonukleotid, der i alt væsentligt omfatter eller består af en sekvens udvalgt fra gruppen bestående af SEQ ID NO: 7 og 49-57, hvor det ene eller flere oligonukleotider er egnede til anvendelse som blokkere; ii) mindst ét oligonukleotid, der i alt væsentligt omfatter eller består af en sekvens udvalgt fra gruppen bestående af SEQ ID NO: 8 og 58- 61, hvor det ene eller flere oligonukleotider er egnede til anvendelse som prober; og iii) en polymerase, fortrinsvis en varmestabil polymerase.
6. Anvendelse af en fremgangsmåde ifølge krav 1 -3 til opdagelse af en methyleringsmarkør, hvor markøren indikerer uønskede hændelser for patienter eller individer, hvor de uønskede hændelser hører til mindst én af følgende kategorier: uønskede lægemiddelinteraktioner; cancersygdomme; cellulære proliferative forstyrrelser; colonkarcinom; leverkarcinom; CNS-dysfunktioner; beskadigelse eller sygdom; symptomer på aggression eller adfærdsforstyrrelser; kliniske, psykologiske og sociale følger af hjerneskader; psykotiske forstyrrelser og personlighedsforstyrrelser; demens og/eller tilknyttede syndromer; kardiovaskulær sygdom, dysfunktion eller beskadigelse; dysfunktion, beskadigelse af eller sygdom i mavetarmkanalen; dysfunktion, beskadigelse af eller sygdom i åndedrætssystemet; læsion, inflammation, infektion, immunitet og/eller rekonvalescens; dysfunktion, beskadigelse af eller sygdom i kroppen som en abnormitet i udviklingsprocessen; dysfunktion, beskadigelse af eller sygdom i hud, muskler, bindevæv eller knogler; endokrin og metabolisk dysfunktion, beskadigelse eller sygdom; hovedpine eller seksuel dysfunktion.
Applications Claiming Priority (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP07109907 | 2007-06-08 | ||
| EP07110409 | 2007-06-15 | ||
| EP07113516 | 2007-07-31 | ||
| EP07114659 | 2007-08-21 | ||
| EP07114863 | 2007-08-23 | ||
| EP08150552 | 2008-01-23 | ||
| EP08151442 | 2008-02-14 | ||
| EP08789067A EP2152902A2 (en) | 2007-06-08 | 2008-06-06 | Method for methylation analysis |
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| DK2479289T3 true DK2479289T3 (da) | 2016-05-02 |
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| DK16163841.6T DK3101141T3 (da) | 2007-06-08 | 2008-06-06 | Fremgangsmåde til methyleringsanalyse |
| DK12164321.7T DK2479289T3 (da) | 2007-06-08 | 2008-06-06 | Fremgangsmåde til methyleringsanalyse |
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| DK16163841.6T DK3101141T3 (da) | 2007-06-08 | 2008-06-06 | Fremgangsmåde til methyleringsanalyse |
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| US (1) | US8623599B2 (da) |
| EP (3) | EP2479289B1 (da) |
| CY (1) | CY1118259T1 (da) |
| DK (2) | DK3101141T3 (da) |
| ES (2) | ES2769841T3 (da) |
| HR (2) | HRP20160425T1 (da) |
| HU (1) | HUE028727T2 (da) |
| LT (1) | LT3101141T (da) |
| PL (1) | PL2479289T3 (da) |
| SI (2) | SI2479289T1 (da) |
| WO (1) | WO2008149237A2 (da) |
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| JP6153866B2 (ja) | 2010-05-25 | 2017-06-28 | キアゲン ガイサーズバーグ アイエヌシー． | 迅速なハイブリッド捕捉アッセイ、及び関連する戦略的に切断されたプローブ |
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| JP6224689B2 (ja) * | 2012-03-15 | 2017-11-01 | ニユー・イングランド・バイオレイブス・インコーポレイテツド | シトシンとこれの修飾物とを識別するための、およびメチローム分析のための方法および組成物 |
| EP2746403A1 (de) | 2012-12-21 | 2014-06-25 | Life Science Inkubator GmbH & Co. KG | Verfahren zur Bestimmung des DNA-Methylierungsgrades |
| CA2902916C (en) | 2013-03-14 | 2018-08-28 | Mayo Foundation For Medical Education And Research | Detecting neoplasm |
| CN105026580A (zh) * | 2013-03-15 | 2015-11-04 | 雅培分子公司 | 重亚硫酸盐转化的核苷酸序列的检测 |
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| US10184154B2 (en) | 2014-09-26 | 2019-01-22 | Mayo Foundation For Medical Education And Research | Detecting cholangiocarcinoma |
| HUE044055T2 (hu) * | 2014-12-19 | 2019-09-30 | Epigenomics Ag | A CPG metiláció kimutatására és a rák diagnosztizálására szolgáló eljárások |
| US10435755B2 (en) | 2015-03-27 | 2019-10-08 | Exact Sciences Development Company, Llc | Detecting esophageal disorders |
| CN114574585A (zh) | 2015-08-31 | 2022-06-03 | 梅约医药教育及研究基金会 | 检测胃肿瘤 |
| CN108350485A (zh) | 2015-10-30 | 2018-07-31 | 精密科学发展有限责任公司 | 血浆dna的多重扩增检测测定以及分离和检测 |
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| CN105506133A (zh) * | 2016-01-15 | 2016-04-20 | 武汉艾米森生命科技有限公司 | 用于检测sept9基因甲基化的组合物、试剂盒及方法 |
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| KR102892245B1 (ko) | 2017-01-27 | 2025-11-27 | 이그젝트 싸이언스 디블롭먼트 컴패니, 엘엘씨 | 메틸화된 dna 분석에 의한 결장 신조직형성의 검출 |
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| ES2570828T3 (es) | 2016-05-20 |
| HRP20200059T1 (hr) | 2020-04-03 |
| SI3101141T1 (sl) | 2020-03-31 |
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| EP2479289A2 (en) | 2012-07-25 |
| EP2152902A2 (en) | 2010-02-17 |
| US20110009277A1 (en) | 2011-01-13 |
| LT3101141T (lt) | 2020-03-10 |
| HRP20160425T1 (hr) | 2016-08-12 |
| US8623599B2 (en) | 2014-01-07 |
| WO2008149237A2 (en) | 2008-12-11 |
| EP2479289B1 (en) | 2016-04-06 |
| DK3101141T3 (da) | 2020-02-03 |
| ES2769841T3 (es) | 2020-06-29 |
| WO2008149237A3 (en) | 2009-06-04 |
| HK1172656A1 (en) | 2013-04-26 |
| SI2479289T1 (sl) | 2016-10-28 |
| CY1118259T1 (el) | 2017-06-28 |
| HUE028727T2 (en) | 2017-01-30 |
| EP3101141A3 (en) | 2017-01-11 |
| EP3101141B1 (en) | 2019-10-16 |
| EP2479289A3 (en) | 2013-06-19 |
| PL2479289T3 (pl) | 2016-10-31 |
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