DK2547771T3 - Fremstilling af proteiner og polypeptider - Google Patents

Fremstilling af proteiner og polypeptider Download PDF

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Publication number
DK2547771T3
DK2547771T3 DK10848078.1T DK10848078T DK2547771T3 DK 2547771 T3 DK2547771 T3 DK 2547771T3 DK 10848078 T DK10848078 T DK 10848078T DK 2547771 T3 DK2547771 T3 DK 2547771T3
Authority
DK
Denmark
Prior art keywords
polypeptides
proteins
preparation
Prior art date
Application number
DK10848078.1T
Other languages
English (en)
Inventor
Jan Johansson
Anna Rising
My Hedhammar
Kerstin Nordling
Original Assignee
Spiber Tech Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/SE2010/050439 external-priority patent/WO2010123450A1/en
Application filed by Spiber Tech Ab filed Critical Spiber Tech Ab
Application granted granted Critical
Publication of DK2547771T3 publication Critical patent/DK2547771T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Insects & Arthropods (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK10848078.1T 2010-03-18 2010-10-27 Fremstilling af proteiner og polypeptider DK2547771T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP10156927 2010-03-18
PCT/SE2010/050439 WO2010123450A1 (en) 2009-04-22 2010-04-21 Method of producing polymers of spider silk proteins
PCT/SE2010/051163 WO2011115538A1 (en) 2010-03-18 2010-10-27 Production of proteins and polypeptides

Publications (1)

Publication Number Publication Date
DK2547771T3 true DK2547771T3 (da) 2017-01-16

Family

ID=42199715

Family Applications (2)

Application Number Title Priority Date Filing Date
DK16190909.8T DK3168229T3 (da) 2010-03-18 2010-10-27 Fremstilling af proteiner og polypeptider.
DK10848078.1T DK2547771T3 (da) 2010-03-18 2010-10-27 Fremstilling af proteiner og polypeptider

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK16190909.8T DK3168229T3 (da) 2010-03-18 2010-10-27 Fremstilling af proteiner og polypeptider.

Country Status (9)

Country Link
US (2) US9644012B2 (da)
EP (2) EP3168229B1 (da)
JP (1) JP5726282B2 (da)
CN (1) CN102844437B (da)
AU (1) AU2010348409B2 (da)
CA (1) CA2792476C (da)
DK (2) DK3168229T3 (da)
ES (1) ES2608673T3 (da)
WO (1) WO2011115538A1 (da)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130281361A1 (en) 2010-11-08 2013-10-24 Pulmatrix, Inc. Methods of treating and preventing rhinovirus infection
US9815869B2 (en) 2012-02-09 2017-11-14 University Of Rochester SP-B and SP-C peptides, synthetic lung surfactants, and use thereof
EP2644619A1 (en) 2012-03-30 2013-10-02 Spiber Technologies AB Monomeric N-terminal spider silk protein domain and uses thereof
US20130303726A1 (en) * 2012-04-17 2013-11-14 Chiesi Farmaceutici S.P.A. Method for the preparation of surfactant peptides
CA2872056A1 (en) * 2012-05-02 2013-11-07 Spiber Technologies Ab Spider silk fusion protein structures without repetitive fragment for binding to an organic target
WO2014056199A1 (zh) * 2012-10-12 2014-04-17 清华大学 多肽的产生和纯化方法
JP2018159137A (ja) 2015-08-20 2018-10-11 国立研究開発法人理化学研究所 クモ糸様構造を有するポリペプチド繊維の製造方法
CN108431027B (zh) * 2015-11-13 2022-07-22 思百博技术股份公司 电荷逆转的n端蜘蛛丝蛋白结构域及其用途
EP3168228A1 (en) 2015-11-13 2017-05-17 Spiber Technologies AB Charge-reversed n-terminal spider silk protein domain and uses thereof
US10717777B2 (en) 2015-12-17 2020-07-21 The Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center Synthetic lung surfactant with enhanced stability and effectiveness
GB201522610D0 (en) * 2015-12-22 2016-02-03 Univ Southampton Protein
KR101652953B1 (ko) * 2016-01-15 2016-08-31 (주)넥스젠바이오텍 열 안정성이 증가한 인간성장호르몬 융합단백질 및 이를 유효성분으로 함유하는 피부 주름 개선 및 탄력 유지용 화장료 조성물
CA3016810C (en) 2016-03-07 2024-04-02 Los Angeles Biomedical Research Institute At Harbor-Ucla Medical Center Compositions and methods for administering ppar.gamma. agonists, surfactant peptides and phospholipids
ES3024660T3 (en) * 2016-06-22 2025-06-05 Amsilk Gmbh Articles comprising a silk polypeptide for antigen delivery
JP2019524874A (ja) * 2016-06-27 2019-09-05 ゲランソン、ウルフ 紐形動物（Ｎｅｍｅｒｔｅａ）門由来の生物活性化合物
US11278577B2 (en) 2016-08-31 2022-03-22 President And Fellows Of Harvard College Engineered bacteria secreting therapeutic proteins and methods of use thereof
JP2020097524A (ja) * 2017-03-07 2020-06-25 Ｓｐｉｂｅｒ株式会社 精製されたタンパク質を製造する方法
US12145971B2 (en) 2017-05-10 2024-11-19 Spiber Inc. Polypeptide solution, production method for polypeptide fiber, and artificial polypeptide
CN111094326B (zh) 2017-06-13 2025-03-04 阿尔托大学基金会 用于产生丝融合蛋白的浓缩粘合相的方法
CN108822196B (zh) * 2018-06-06 2023-04-21 湖南生达生物科技有限公司 一种促凝血多肽lgtx-f2及其应用
CN112359059B (zh) * 2020-11-10 2021-11-30 北部湾大学 用于表达rFC蛋白的84E突变载体及其制备方法和应用
CN114159546B (zh) * 2021-12-20 2024-06-04 北京双鹤润创科技有限公司 新一代合成肺表面活性物质制剂及其临床应用
WO2023171835A1 (ko) * 2022-03-10 2023-09-14 포항공과대학교 산학협력단 식물에서 생산된 factor c의 sushi domain을 포함하는 재조합 단백질을 이용한 엔도톡신 lps 제거 방법
WO2025198514A1 (en) * 2024-03-21 2025-09-25 Anna Rising Recombinant spider silk proteins

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR9610058A (pt) * 1995-07-28 1999-07-27 Marie Curie Cancer Care Uso de vp22 ou uma porção ativa fragmento ou homólogo do mesmo proteína de transporte ácido nucleíco vetor de expressão célula hospedeira de mamíferos ou microbiana e processos para transportar uma proteína ou peptídeo desejado para uma população de marcaç o de células e para transportar uma molécula desejada em uma população de células
AU3643497A (en) * 1996-06-28 1998-01-21 National Jewish Center For Immunology And Respiratory Medicine Use of thioredoxin-like molecules for induction of mnsod to treat oxidative damage
US6017735A (en) * 1997-01-23 2000-01-25 Marie Curie Cancer Care Materials and methods for intracellular transport and their uses
AU2002312426A1 (en) 2001-06-06 2002-12-16 University Of Wyoming Expression of spider silk proteins in higher plants
WO2003057727A1 (en) 2002-01-11 2003-07-17 Nexia Biotechnologies, Inc. Methods of producing silk polypeptides and products thereof
US20050261479A1 (en) * 2004-04-29 2005-11-24 Christian Hoffmann Method for purifying and recovering silk proteins using magnetic affinity separation
EP2899204B1 (en) 2005-12-30 2017-06-28 Spiber Technologies AB Support for cell culture comprising spider silk proteins and methods for producing the same
BRPI0701826B1 (pt) 2007-03-16 2021-02-17 Embrapa - Empresa Brasileira De Pesquisa Agropecuária proteínas da teia de aranha nephilengys cruentata, avicularia juruensis e parawixia bistriata isoladas da biodiversidade brasileira
WO2008154547A2 (en) 2007-06-11 2008-12-18 The Regents Of The University Of California Spider silk dragline polynucleotides, polypeptides and methods of use thereof
EP2243792A1 (en) * 2009-04-22 2010-10-27 Spiber Technologies AB Methods of producing polymers of spider silk proteins
CA2759465C (en) 2009-04-22 2017-12-12 Spiber Technologies Ab Method of producing polymers of spider silk proteins

Also Published As

Publication number Publication date
JP2013521801A (ja) 2013-06-13
CA2792476A1 (en) 2011-09-22
EP2547771A1 (en) 2013-01-23
WO2011115538A1 (en) 2011-09-22
EP2547771B8 (en) 2017-07-19
EP2547771B1 (en) 2016-09-28
CN102844437B (zh) 2015-06-03
AU2010348409B2 (en) 2014-03-20
DK3168229T3 (da) 2019-09-23
EP3168229B1 (en) 2019-06-19
EP3168229A1 (en) 2017-05-17
AU2010348409A1 (en) 2012-09-27
US9644012B2 (en) 2017-05-09
US20130065278A1 (en) 2013-03-14
JP5726282B2 (ja) 2015-05-27
EP2547771A4 (en) 2014-01-01
CN102844437A (zh) 2012-12-26
CA2792476C (en) 2018-12-11
US20170283474A1 (en) 2017-10-05
ES2608673T3 (es) 2017-04-12
US10604550B2 (en) 2020-03-31

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