DK3011029T3 - Administration, modificering og optimering af tandem-guidesystemer, fremgangsmåder og sammensætninger til sekvensmanipulering - Google Patents

Administration, modificering og optimering af tandem-guidesystemer, fremgangsmåder og sammensætninger til sekvensmanipulering Download PDF

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DK3011029T3
DK3011029T3 DK14734708.2T DK14734708T DK3011029T3 DK 3011029 T3 DK3011029 T3 DK 3011029T3 DK 14734708 T DK14734708 T DK 14734708T DK 3011029 T3 DK3011029 T3 DK 3011029T3
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optimization
administration
procedures
compositions
modification
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DK14734708.2T
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Feng Zhang
Chie-Yu Lin
Fei Ran
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Broad Inst Inc
Massachusetts Inst Technology
Harvard College
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
    • C—CHEMISTRY; METALLURGY
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Medicinal Chemistry (AREA)
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  • Bioinformatics & Computational Biology (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
DK14734708.2T 2013-06-17 2014-06-10 Administration, modificering og optimering af tandem-guidesystemer, fremgangsmåder og sammensætninger til sekvensmanipulering DK3011029T3 (da)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201361836123P 2013-06-17 2013-06-17
US201361847537P 2013-07-17 2013-07-17
US201361862355P 2013-08-05 2013-08-05
US201361862468P 2013-08-05 2013-08-05
US201361871301P 2013-08-28 2013-08-28
US201361915407P 2013-12-12 2013-12-12
US201461979942P 2014-04-15 2014-04-15
PCT/US2014/041800 WO2014204724A1 (en) 2013-06-17 2014-06-10 Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation

Publications (1)

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DK3011029T3 true DK3011029T3 (da) 2020-03-16

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Country Status (10)

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US (2) US11008588B2 (2)
EP (2) EP3011029B1 (2)
JP (2) JP6625971B2 (2)
KR (1) KR20160044457A (2)
CN (1) CN106062197A (2)
AU (1) AU2014281026B2 (2)
CA (1) CA2915834A1 (2)
DK (1) DK3011029T3 (2)
ES (1) ES2777217T3 (2)
WO (1) WO2014204724A1 (2)

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US20160186213A1 (en) 2016-06-30
JP2020054354A (ja) 2020-04-09
EP3674411A1 (en) 2020-07-01
ES2777217T3 (es) 2020-08-04
WO2014204724A8 (en) 2015-12-23
JP2016521993A (ja) 2016-07-28
EP3011029A1 (en) 2016-04-27
AU2014281026A1 (en) 2016-01-28
AU2014281026A9 (en) 2016-06-16
HK1223646A1 (en) 2017-08-04
US11008588B2 (en) 2021-05-18
AU2014281026B2 (en) 2020-05-28
JP6625971B2 (ja) 2019-12-25
WO2014204724A1 (en) 2014-12-24
EP3011029B1 (en) 2019-12-11
CA2915834A1 (en) 2014-12-24
KR20160044457A (ko) 2016-04-25
WO2014204724A9 (en) 2016-03-24
US20210292794A1 (en) 2021-09-23

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