EA201400995A1 - Способы повышения выносливости к тепловому стрессу и содержания аминокислот в растениях - Google Patents

Способы повышения выносливости к тепловому стрессу и содержания аминокислот в растениях

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Publication number
EA201400995A1
EA201400995A1 EA201400995A EA201400995A EA201400995A1 EA 201400995 A1 EA201400995 A1 EA 201400995A1 EA 201400995 A EA201400995 A EA 201400995A EA 201400995 A EA201400995 A EA 201400995A EA 201400995 A1 EA201400995 A1 EA 201400995A1
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EA
Eurasian Patent Office
Prior art keywords
plant
increasing
methods
heat stress
endurance
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Application number
EA201400995A
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English (en)
Inventor
Стивен Ротстейн
Юнмэй Би
Асхраф Эль-Кереами
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Юниверсити Оф Гуэлф
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Application filed by Юниверсити Оф Гуэлф filed Critical Юниверсити Оф Гуэлф
Publication of EA201400995A1 publication Critical patent/EA201400995A1/ru

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    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Изобретение предусматривает способы повышения выносливости к высокой температуре или тепловому стрессу у растения, части растения или растительной клетки, причем способ включает введение одной или нескольких нуклеиновых кислот, кодирующих (i) глутаминсинтетазу 1; 2 (GS1; 2); (ii) глутаматдекарбоксилазу 3 (GAD3); (iii) глутаминамидотрансферазу класса I (GAT1); (iv) полипептид MYB55 или любую их комбинацию, в растение, часть растения или растительную клетку. Также предусматриваются способы повышения содержания аминокислот в растении, части растения или растительной клетке, причем способ включает введение одной или нескольких нуклеиновых кислот, кодирующих (i) GS1; 2, (ii) GAD3, (iii) GAT1, (iv) полипептид MYB55 или любую их комбинацию, в растение, часть растения или растительную клетку.
EA201400995A 2012-03-13 2013-03-13 Способы повышения выносливости к тепловому стрессу и содержания аминокислот в растениях EA201400995A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261610288P 2012-03-13 2012-03-13
PCT/IB2013/051975 WO2013136273A2 (en) 2012-03-13 2013-03-13 Methods of increasing tolerance to heat stress and amino acid content of plants

Publications (1)

Publication Number Publication Date
EA201400995A1 true EA201400995A1 (ru) 2015-02-27

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US (1) US20150218578A1 (ru)
CN (1) CN104302773A (ru)
AU (1) AU2013233946A1 (ru)
CA (1) CA2865531A1 (ru)
EA (1) EA201400995A1 (ru)
HU (1) HUP1400495A2 (ru)
MX (1) MX2014010988A (ru)
WO (1) WO2013136273A2 (ru)

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US20150203864A1 (en) * 2012-03-13 2015-07-23 University Of Guelph Myb55 promoter and use thereof
CN104846008B (zh) * 2015-05-07 2017-11-28 安徽农业大学 Gs1;2基因在调控植物根长中的应用
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CN105861519B (zh) * 2016-05-20 2018-11-06 中国热带农业科学院橡胶研究所 橡胶树转录因子HbMYB44基因及其应用
CN109913470B (zh) * 2019-03-27 2022-05-20 西南大学 超量表达甘蓝myb55在甘蓝型油菜分子育种中的应用
CN109777825B (zh) * 2019-03-27 2022-05-20 西南大学 超量表达甘蓝型油菜myb55在甘蓝型油菜分子育种中的应用
CN109762840B (zh) * 2019-03-27 2020-06-26 西南大学 超量表达白菜myb55在甘蓝型油菜分子育种中的应用
CN110041126A (zh) * 2019-04-11 2019-07-23 安徽农业大学 一种观赏类花卉专用的耐热性药剂
CN110183523B (zh) * 2019-05-30 2021-02-09 中国农业科学院生物技术研究所 OsMYB36蛋白及其编码基因和应用
CN111499709B (zh) * 2020-05-19 2022-02-18 中国农业大学 水稻穗粒数相关的rgn1蛋白及其编码基因与应用
CN111662890B (zh) * 2020-07-27 2023-03-24 洛阳师范学院 一种OsProDH基因及其在负调控水稻耐热性方面的应用
CN114410649A (zh) * 2021-12-29 2022-04-29 浙江农林大学 一种热胁迫下水稻内参基因及其应用
CN114703219B (zh) * 2022-01-24 2023-10-24 浙江农林大学暨阳学院 一种杜鹃热激转录因子Hsfs调控耐热能力的检测分析方法
CN119552910B (zh) * 2025-01-20 2025-05-16 中国农业大学 TaMYB55蛋白及其编码基因在调控植物耐热性中的应用
CN120924592B (zh) * 2025-10-15 2026-03-20 江西农业大学 AhHTF1基因在提高植物耐高温能力中的应用

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Publication number Publication date
WO2013136273A2 (en) 2013-09-19
MX2014010988A (es) 2014-12-08
WO2013136273A3 (en) 2013-12-05
US20150218578A1 (en) 2015-08-06
CN104302773A (zh) 2015-01-21
AU2013233946A1 (en) 2014-10-09
CA2865531A1 (en) 2013-09-19
HUP1400495A2 (en) 2015-03-02

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