EP1021462A4 - TESTS FOR NUCLEAR RECEPTOR LIGANDS BASED ON TRANSFER OF RESONANCE ENERGY IN FLUORESCENCE - Google Patents

TESTS FOR NUCLEAR RECEPTOR LIGANDS BASED ON TRANSFER OF RESONANCE ENERGY IN FLUORESCENCE

Info

Publication number
EP1021462A4
EP1021462A4 EP98956094A EP98956094A EP1021462A4 EP 1021462 A4 EP1021462 A4 EP 1021462A4 EP 98956094 A EP98956094 A EP 98956094A EP 98956094 A EP98956094 A EP 98956094A EP 1021462 A4 EP1021462 A4 EP 1021462A4
Authority
EP
European Patent Office
Prior art keywords
fluorescence
tests
transfer
resonance energy
nuclear receptor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP98956094A
Other languages
German (de)
French (fr)
Other versions
EP1021462A1 (en
Inventor
Richard T Cummings
Jeffrey D Hermes
David E Moller
Gaochao Zhou
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck and Co Inc
Original Assignee
Merck and Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck and Co Inc filed Critical Merck and Co Inc
Publication of EP1021462A1 publication Critical patent/EP1021462A1/en
Publication of EP1021462A4 publication Critical patent/EP1021462A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6875—Nucleoproteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70567—Nuclear receptors, e.g. retinoic acid receptor [RAR], RXR, nuclear orphan receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
EP98956094A 1997-10-07 1998-10-06 TESTS FOR NUCLEAR RECEPTOR LIGANDS BASED ON TRANSFER OF RESONANCE ENERGY IN FLUORESCENCE Withdrawn EP1021462A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US6138597P 1997-10-07 1997-10-07
US61385P 1997-10-07
PCT/US1998/021049 WO1999018124A1 (en) 1997-10-07 1998-10-06 Assays for nuclear receptor ligands using fret

Publications (2)

Publication Number Publication Date
EP1021462A1 EP1021462A1 (en) 2000-07-26
EP1021462A4 true EP1021462A4 (en) 2005-04-13

Family

ID=22035450

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98956094A Withdrawn EP1021462A4 (en) 1997-10-07 1998-10-06 TESTS FOR NUCLEAR RECEPTOR LIGANDS BASED ON TRANSFER OF RESONANCE ENERGY IN FLUORESCENCE

Country Status (4)

Country Link
EP (1) EP1021462A4 (en)
JP (1) JP2001519525A (en)
CA (1) CA2305711A1 (en)
WO (1) WO1999018124A1 (en)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002528721A (en) * 1998-10-23 2002-09-03 グラクソ グループ リミテッド Assays for nuclear receptor ligands
JP2002532729A (en) * 1998-12-23 2002-10-02 グラクソ グループ リミテッド Assay for nuclear receptor ligands
AU780935B2 (en) * 1999-09-13 2005-04-28 Equitech Laboratories, Inc. Materials and methods for the determination of an analyte
EP1238285A1 (en) * 1999-11-30 2002-09-11 Akzo Nobel N.V. Steroid compounds for steroid receptor binding assays
WO2001071352A2 (en) * 2000-03-17 2001-09-27 The Salk Institute For Biological Studies Compositions associated with complex formation
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6924311B2 (en) 2001-10-17 2005-08-02 X-Ceptor Therapeutics, Inc. Methods for affecting various diseases utilizing LXR compounds
EP1310799B1 (en) 2001-11-07 2005-12-21 Schering AG In vitro screening for ligands of the estrogen receptor
AU2003252159A1 (en) * 2002-07-24 2004-02-09 Johnson & Johnson Pharmaceutical Research & Development, L.L.C. Method for the identification of ligands
CA2505515C (en) 2002-11-07 2013-08-27 Erasmus Universiteit Rotterdam Fret probes and methods for detecting interacting molecules
JP4803976B2 (en) * 2003-07-09 2011-10-26 独立行政法人科学技術振興機構 Molecular sensor for intracellular IP3 measurement
CA2537099A1 (en) 2003-08-28 2005-03-10 Choongwae Pharma Corporation Modulation of .beta.-catenin/tcf activated transcription
WO2005054438A2 (en) 2003-12-01 2005-06-16 Invitrogen Corporation Nucleic acid molecules containing recombination sites and methods of using the same
US20080286260A1 (en) * 2004-03-26 2008-11-20 Bayer Healthcare Ag Diagnostic and Therapeutics for Diseases Associated with Peroxisome Proliferative Activated Receptor Alpha (Ppara)
KR100704011B1 (en) 2005-02-16 2007-04-04 한국과학기술원 Biomolecule-specific binding detection method by FTR of metal nanoparticles and quantum dots
US7989179B2 (en) 2005-06-28 2011-08-02 Daiichi Sankyo Company, Limited LXR ligand testing method
JP5264421B2 (en) 2007-11-22 2013-08-14 富士フイルム株式会社 Test substance measuring carrier and method for producing the same
WO2009141926A1 (en) * 2008-05-23 2009-11-26 国立大学法人東京大学 Method for acquisition of compound capable of acting on glucose metabolism/lipid metabolism
WO2015159292A2 (en) * 2014-04-14 2015-10-22 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. A method and kit for determining the tissue or cell origin of dna
CN110168099B (en) * 2016-06-07 2024-06-07 加利福尼亚大学董事会 Cell-free DNA methylation patterns for disease and condition analysis

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997040378A1 (en) * 1996-04-19 1997-10-30 Vereniging Het Nederlands Kanker Instituut Interaction of cyclin d1 and estrogen receptor and its use in assays
WO1998056806A1 (en) * 1997-06-12 1998-12-17 The Regents Of The University Of California A TRANSCRIPTION FACTOR COACTIVATOR PROTEIN, p/CIP
WO1999041608A2 (en) * 1998-02-12 1999-08-19 Prolifix Limited Interaction between cyclin d1 and steroid receptor co-activators

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997040378A1 (en) * 1996-04-19 1997-10-30 Vereniging Het Nederlands Kanker Instituut Interaction of cyclin d1 and estrogen receptor and its use in assays
WO1998056806A1 (en) * 1997-06-12 1998-12-17 The Regents Of The University Of California A TRANSCRIPTION FACTOR COACTIVATOR PROTEIN, p/CIP
WO1999041608A2 (en) * 1998-02-12 1999-08-19 Prolifix Limited Interaction between cyclin d1 and steroid receptor co-activators

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
CLINICAL CHEMISTRY, vol. 43, no. 6 PART 2, 1997, 49TH ANNUAL MEETING OF THE AMERICAN ASSOCIATION FOR CLINICAL CHEMISTRY; ATLANTA, GEORGIA, USA; JULY 20-24, 1997, pages S159, ISSN: 0009-9147 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 22 July 1997 (1997-07-22), BELLANGER L ET AL: "Homogeneous time-resolved fluoroimmunoassay for cardiac troponin-I using TRACE technology", XP008042758, Database accession no. PREV199799633726 *
KOLB A J ET AL: "HOMOGENEOUS, TIME-RESOLVED FLUORESCENCE METHOD FOR DRUG DISCOVERY", 1997, HIGH-THROUGHPUT SCREENING, THE DISCOVERY OF BIOACTIVE SUBSTANCES, XX, XX, PAGE(S) 345-360, XP008042717 *
KOLB J M ET AL: "USE OF A NOVEL HOMOGENEOUS FLUORESCENT TECHNOLOGY IN HIGH THROUGHPUT SCREENING", JOURNAL OF BIOMOLECULAR SCREENING, LARCHMONT, NY, US, vol. 1, no. 4, 1996, pages 203 - 210, XP008042702, ISSN: 1087-0571 *
LEE S P ET AL: "CHARACTERIZATION OF ENDONUCLEOLYTIC ACTIVITY OF HIV-1 INTEGRASE USING A FLUOROGENIC SUBSTRATE", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 227, 1995, pages 295 - 301, XP002945389, ISSN: 0003-2697 *
MATHIS G: "PROBING MOLECULAR INTERACTIONS WITH HOMOGENEOUS TECHNIQUES BASED ONRARE EARTH CRYPTATES AND FLUORESCENCE ENERGY TRANSFER", CLINICAL CHEMISTRY, vol. 41, no. 9, 1995, pages 1391 - 1397, XP001018128, ISSN: 0009-9147 *
ROGERS M V: "LIGHT ON HIGH-THROUGHPUT SCREENING: FLUORESCENCE-BASED ASSAY TECHNOLOGIES", DRUG DISCOVERY TODAY, ELSEVIER SCIENCE LTD, GB, vol. 2, no. 4, April 1997 (1997-04-01), pages 156 - 160, XP002916138, ISSN: 1359-6446 *
ROGERS: "Light on high-throughput screening: fluorescence-based assay technologies", DRUG DISCOVERY TODAY, vol. 2, no. 4, April 1997 (1997-04-01), pages 156 - 160, XP002916138 *
See also references of WO9918124A1 *
VOEGEL, HEINE, ZECHEL, CHAMBON, GRONEMEYER: "TIF2, a 160 kDa transcriptional mediator for the ligand-dependent activation function AF-2 of nuclear receptors", EMBO JOURNAL, vol. 15, no. 14, 1996, pages 3667 - 3675, XP002045757 *
ZHOU G ET AL: "Use of homogeneous time-resolved fluorescence energy transfer in the measurement of nuclear receptor activation", METHODS : A COMPANION TO METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC., NEW YORK, NY, US, vol. 25, no. 1, September 2001 (2001-09-01), pages 54 - 61, XP002267175, ISSN: 1046-2023 *

Also Published As

Publication number Publication date
CA2305711A1 (en) 1999-04-15
WO1999018124A1 (en) 1999-04-15
JP2001519525A (en) 2001-10-23
EP1021462A1 (en) 2000-07-26

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