EP1194574A4 - High efficiency transfection based on low electric field strength, long pulse length - Google Patents

High efficiency transfection based on low electric field strength, long pulse length

Info

Publication number: EP1194574A4
Authority: EP; European Patent Office
Prior art keywords: electric field; high efficiency; field strength; pulse length; low electric
Prior art date: 1999-06-25
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Ceased

Application number

EP99928868A

Other languages

German (de)

French (fr)

Other versions

EP1194574A1 (en

Inventor

Ed Nolan

Robin Filshie

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Genetronics Inc

Original Assignee

Genetronics Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1999-06-25

Filing date

1999-06-25

Publication date

2003-04-02

1999-06-25 Application filed by Genetronics Inc filed Critical Genetronics Inc

1999-06-25 Priority to EP07010185A priority Critical patent/EP1829973A3/en

2002-04-10 Publication of EP1194574A1 publication Critical patent/EP1194574A1/en

2003-04-02 Publication of EP1194574A4 publication Critical patent/EP1194574A4/en

Status Ceased legal-status Critical Current

Links

230000005684 electric field Effects 0.000 title 1
238000001890 transfection Methods 0.000 title 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

Genetics & Genomics (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Wood Science & Technology (AREA)
Organic Chemistry (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Apparatus Associated With Microorganisms And Enzymes (AREA)

EP99928868A 1999-06-25 1999-06-25 High efficiency transfection based on low electric field strength, long pulse length Ceased EP1194574A4 (en)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
EP07010185A EP1829973A3 (en)	1999-06-25	1999-06-25	High efficienty transfection based on low electric field strength, low pulse length

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
PCT/US1999/014447 WO2001000856A1 (en)	1999-06-25	1999-06-25	High efficiency transfection based on low electric field strength, long pulse length

Related Child Applications (1)

Application Number	Title	Priority Date	Filing Date
EP07010185A Division EP1829973A3 (en)	1999-06-25	1999-06-25	High efficienty transfection based on low electric field strength, low pulse length

Publications (2)

Publication Number	Publication Date
EP1194574A1 EP1194574A1 (en)	2002-04-10
EP1194574A4 true EP1194574A4 (en)	2003-04-02

Family

ID=22273056

Family Applications (2)

Application Number	Title	Priority Date	Filing Date
EP07010185A Withdrawn EP1829973A3 (en)	1999-06-25	1999-06-25	High efficienty transfection based on low electric field strength, low pulse length
EP99928868A Ceased EP1194574A4 (en)	1999-06-25	1999-06-25	High efficiency transfection based on low electric field strength, long pulse length

Family Applications Before (1)

Application Number	Title	Priority Date	Filing Date
EP07010185A Withdrawn EP1829973A3 (en)	1999-06-25	1999-06-25	High efficienty transfection based on low electric field strength, low pulse length

Country Status (4)

Country	Link
EP (2)	EP1829973A3 (en)
AU (1)	AU783432B2 (en)
CA (1)	CA2377565A1 (en)
WO (1)	WO2001000856A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CA2497343C (en)	2001-08-14	2013-04-02	Washington University In St. Louis	Systems and methods for screening pharmaceutical chemicals
US20130177988A1 (en) *	2011-11-17	2013-07-11	Brigham Young University	Cytoplasm to organelle delivery system and associated methods

Citations (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1997007826A1 (en) *	1995-08-29	1997-03-06	Cbr Laboratories, Inc.	In vivo electroporation of cells
WO1999001158A1 (en) *	1997-06-30	1999-01-14	Rhone-Poulenc Rorer S.A.	Improved method for transferring nucleic acid into the striped muscle and combination therefor

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE68925030T2 (en) *	1988-01-21	1996-07-25	Massachusetts Inst Technology	MOLECULE TRANSPORT THROUGH FABRICS WITH THE USE OF ELECTROPORATION.
US5944710A (en) *	1996-06-24	1999-08-31	Genetronics, Inc.	Electroporation-mediated intravascular delivery
IL133708A0 (en) *	1997-06-30	2001-04-30	Rhone Poulence Rorer S A	Improved method for transferring nucleic acid into multicelled eukaryotic organism cells and combination thereof

1999
- 1999-06-25 WO PCT/US1999/014447 patent/WO2001000856A1/en not_active Ceased
- 1999-06-25 CA CA002377565A patent/CA2377565A1/en not_active Abandoned
- 1999-06-25 EP EP07010185A patent/EP1829973A3/en not_active Withdrawn
- 1999-06-25 AU AU45842/99A patent/AU783432B2/en not_active Ceased
- 1999-06-25 EP EP99928868A patent/EP1194574A4/en not_active Ceased

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1997007826A1 (en) *	1995-08-29	1997-03-06	Cbr Laboratories, Inc.	In vivo electroporation of cells
WO1999001158A1 (en) *	1997-06-30	1999-01-14	Rhone-Poulenc Rorer S.A.	Improved method for transferring nucleic acid into the striped muscle and combination therefor

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
AIHARA H ET AL: "GENE TRANSFER INTO MUSCLE BY ELECTROPORATION IN VIVO", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 16, no. 9, September 1998 (1998-09-01), pages 867 - 870, XP001061636, ISSN: 1087-0156 *
See also references of WO0100856A1 *

Also Published As

Publication number	Publication date
CA2377565A1 (en)	2001-01-04
EP1194574A1 (en)	2002-04-10
AU783432B2 (en)	2005-10-27
EP1829973A2 (en)	2007-09-05
AU4584299A (en)	2001-01-31
EP1829973A3 (en)	2008-01-09
WO2001000856A1 (en)	2001-01-04

Legal Events

Date	Code	Title	Description
2002-02-22	PUAI	Public reference made under article 153(3) epc to a published international application that has entered the european phase	Free format text: ORIGINAL CODE: 0009012
2002-04-10	17P	Request for examination filed	Effective date: 20020118
2002-04-10	AK	Designated contracting states	Kind code of ref document: A1 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
2003-04-02	A4	Supplementary search report drawn up and despatched	Effective date: 20030218
2005-07-27	17Q	First examination report despatched	Effective date: 20050613
2007-10-05	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: THE APPLICATION HAS BEEN REFUSED
2007-11-07	18R	Application refused	Effective date: 20070524

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