EP1294221A4 - Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection - Google Patents

Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection

Info

Publication number
EP1294221A4
EP1294221A4 EP01918263A EP01918263A EP1294221A4 EP 1294221 A4 EP1294221 A4 EP 1294221A4 EP 01918263 A EP01918263 A EP 01918263A EP 01918263 A EP01918263 A EP 01918263A EP 1294221 A4 EP1294221 A4 EP 1294221A4
Authority
EP
European Patent Office
Prior art keywords
engineering
transgenic plants
chloroplast genome
antibiotic selection
marker free
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP01918263A
Other languages
German (de)
French (fr)
Other versions
EP1294221A1 (en
Inventor
Henry Daniell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Auburn University
University of Central Florida
Original Assignee
Auburn University
University of Central Florida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Auburn University, University of Central Florida filed Critical Auburn University
Publication of EP1294221A1 publication Critical patent/EP1294221A1/en
Publication of EP1294221A4 publication Critical patent/EP1294221A4/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Catching Or Destruction (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
EP01918263A 2000-03-02 2001-02-28 Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection Ceased EP1294221A4 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US18630800P 2000-03-02 2000-03-02
US186308P 2000-03-02
US20876300P 2000-06-02 2000-06-02
US208763P 2000-06-04
US25740600P 2000-12-22 2000-12-22
US257406P 2000-12-22
US25915400P 2000-12-28 2000-12-28
US259154P 2000-12-29
PCT/US2001/006275 WO2001064023A1 (en) 2000-03-02 2001-02-28 Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection

Publications (2)

Publication Number Publication Date
EP1294221A1 EP1294221A1 (en) 2003-03-26
EP1294221A4 true EP1294221A4 (en) 2003-06-25

Family

ID=27497669

Family Applications (1)

Application Number Title Priority Date Filing Date
EP01918263A Ceased EP1294221A4 (en) 2000-03-02 2001-02-28 Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection

Country Status (4)

Country Link
EP (1) EP1294221A4 (en)
AU (1) AU2001245359A1 (en)
CA (1) CA2401954A1 (en)
WO (1) WO2001064023A1 (en)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100251425A9 (en) 1998-05-15 2010-09-30 University Of Central Florida Expression of human interferon in transgenic chloroplasts
EP1274846A4 (en) 2000-03-01 2004-09-29 Univ Auburn Plastid transformation vectors for expressing human proteins in plants
ATE500336T1 (en) 2001-12-26 2011-03-15 Univ Central Florida EXPRESSION OF PROTECTIVE ANTIGENS IN TRANSGENIC CHLOROPLASTS AND PRODUCTION OF IMPROVED VACCINES
EP2308985B1 (en) * 2002-07-03 2018-06-06 The Trustees of the University of Pennsylvania Plastid genetic engineering via somatic embryogenesis
WO2004013333A2 (en) 2002-07-26 2004-02-12 Basf Plant Science Gmbh Inversion of the negative-selective effect of negative marker proteins using selection methods
EP1565561A1 (en) * 2002-11-28 2005-08-24 Universiteit Leiden A method for marker-less integration of a sequence of interest into the genome of a cell
WO2004081184A2 (en) 2003-03-07 2004-09-23 Seminis Vegetable Seeds, Inc. Markerless transformation
US20090007294A1 (en) * 2004-09-01 2009-01-01 Henry Daniell Genetic Engineering of Male Sterility in Plants
US20080274143A1 (en) 2005-05-27 2008-11-06 Henry Daniell Chloroplasts Engineering to Express Pharmaceutical Proteins
BRPI0617006A8 (en) 2005-09-16 2017-01-24 Bayer Cropscience Sa CHIMERIC GENE, VECTOR DESIGNATED FOR TRANSFORMATION OF PLASTID PLASTIDS, PLASTOMIC PLANT CELL, TRANSPLASTOMIC PLANT, HARVEST PARTS OF PLANT, PRODUCTION METHOD OF PROTEIN OF INTEREST IN PLANT CELL AND PEPTIDE PRODUCTION METHODS
DE602007012269D1 (en) 2006-08-31 2011-03-10 Monsanto Technology Llc PROCESS FOR PRODUCING TRANSGENIC PLANTS
GB2447416B (en) * 2006-12-15 2010-08-25 Uni I Stavanger Methods and vectors for transformation of plastids and marker excision using site-specific recombination
US10752909B2 (en) 2007-03-30 2020-08-25 The Trustees Of The University Of Pennsylvania Chloroplasts engineered to express pharmaceutical proteins in edible plants
WO2015058214A1 (en) 2013-10-18 2015-04-23 Trustees Of The University Of Pennsylvania Oral delivery of angiotensin converting enzyme 2 (ace2) or angiotensin-(1-7) bioencapsulated in plant cells
PT3068869T (en) 2013-11-15 2020-10-08 Univ Pennsylvania Compositions and methods for suppression of inhibitor formation against factor viii in hemophilia a patients
MA41180A (en) 2014-12-17 2017-10-24 Bayer Cropscience Nv PLANTS CHARACTERIZED BY IMPROVED PHOTOSYNTHETIC CARBON BINDING CAPACITY
WO2017038835A2 (en) * 2015-08-28 2017-03-09 Honda Motor Co., Ltd. Method of producing plastid transformants
JP6696806B2 (en) * 2015-08-28 2020-05-20 本田技研工業株式会社 Method for producing plastid transformant
AU2016355563A1 (en) 2015-11-16 2018-06-21 The Trustees Of The University Of Pennsylvania Targeted delivery of therapeutic proteins bioencapsulated in plant cells to cell types of interest for the treatment of disease
CN109554445B (en) * 2019-01-23 2021-09-21 山东省花生研究所（山东省农业科学院花生工程技术研究中心） Effective and simple method for analyzing genetic relationship between peanut species

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999010513A1 (en) * 1997-08-07 1999-03-04 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
WO2000003022A2 (en) * 1998-07-10 2000-01-20 Calgene Llc Expression of herbicide tolerance genes in plant plastids

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5877402A (en) * 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
US5633153A (en) * 1994-10-14 1997-05-27 Calgene, Inc. Aldehyde dehydrogenase selectable markers for plant transformation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999010513A1 (en) * 1997-08-07 1999-03-04 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
WO2000003022A2 (en) * 1998-07-10 2000-01-20 Calgene Llc Expression of herbicide tolerance genes in plant plastids

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KUMAR ET AL: "Plastid-Expressed Betaine Aldehyde Dehydrogenase Gene in Carrot Cultured Cells, Roots, and Leaves Confers Enhanced Salt Tolerance", PLANT PHYSIOLOGY, vol. 136, September 2004 (2004-09-01), pages 2843 - 2854 *
See also references of WO0164023A1 *

Also Published As

Publication number Publication date
CA2401954A1 (en) 2001-09-07
AU2001245359A1 (en) 2001-09-12
EP1294221A1 (en) 2003-03-26
WO2001064023B1 (en) 2001-12-13
WO2001064023A1 (en) 2001-09-07

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