EP1448763A4 - Characterization and isolation of subsets of human embryonic stem cells (hes) and cells associated or derived therefrom - Google Patents

Characterization and isolation of subsets of human embryonic stem cells (hes) and cells associated or derived therefrom

Info

Publication number
EP1448763A4
EP1448763A4 EP02774172A EP02774172A EP1448763A4 EP 1448763 A4 EP1448763 A4 EP 1448763A4 EP 02774172 A EP02774172 A EP 02774172A EP 02774172 A EP02774172 A EP 02774172A EP 1448763 A4 EP1448763 A4 EP 1448763A4
Authority
EP
European Patent Office
Prior art keywords
hes
subsets
characterization
isolation
embryonic stem
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP02774172A
Other languages
German (de)
French (fr)
Other versions
EP1448763A1 (en
Inventor
Martin Frederick Pera
Andrew Laslett
Susan Hawes
Tomonobu Gion
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ES Cell International Pte Ltd
Original Assignee
ES Cell International Pte Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AUPR8781A external-priority patent/AUPR878101A0/en
Priority claimed from AUPS1129A external-priority patent/AUPS112902A0/en
Application filed by ES Cell International Pte Ltd filed Critical ES Cell International Pte Ltd
Publication of EP1448763A1 publication Critical patent/EP1448763A1/en
Publication of EP1448763A4 publication Critical patent/EP1448763A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP02774172A 2001-11-09 2002-11-11 Characterization and isolation of subsets of human embryonic stem cells (hes) and cells associated or derived therefrom Withdrawn EP1448763A4 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
AUPR878101 2001-11-09
AUPR8781A AUPR878101A0 (en) 2001-11-09 2001-11-09 Characterization and isolation of subsets of human embryonic stem cellss (HES) and cells associated or derived therefrom
AUPS1129A AUPS112902A0 (en) 2002-03-15 2002-03-15 Characterisation and isolation of subsets of human embryonic stem cells and progenitor cells derived therefrom (2)
AUPS112902 2002-03-15
PCT/AU2002/001534 WO2003040355A1 (en) 2001-11-09 2002-11-11 Characterization and isolation of subsets of human embryonic stem cells (hes) and cells associated or derived therefrom

Publications (2)

Publication Number Publication Date
EP1448763A1 EP1448763A1 (en) 2004-08-25
EP1448763A4 true EP1448763A4 (en) 2006-11-29

Family

ID=25646839

Family Applications (1)

Application Number Title Priority Date Filing Date
EP02774172A Withdrawn EP1448763A4 (en) 2001-11-09 2002-11-11 Characterization and isolation of subsets of human embryonic stem cells (hes) and cells associated or derived therefrom

Country Status (4)

Country Link
EP (1) EP1448763A4 (en)
CA (1) CA2466342A1 (en)
GB (2) GB2419890A (en)
WO (1) WO2003040355A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL162131A0 (en) 2001-12-07 2005-11-20 Geron Corp Islet cells from human embryonic stem cells
US8119774B2 (en) 2003-10-09 2012-02-21 Martin Pera Cell marker for hepatic and pancreatic stem cells and progenitor cells
AU2005230832B2 (en) * 2004-04-01 2010-11-11 Wisconsin Alumni Research Foundation Differentiation of stem cells to endoderm and pancreatic lineage
WO2006084314A1 (en) * 2005-02-09 2006-08-17 Australian Stem Cell Centre Limited Stem cell populations and classification system
AU2006304390A1 (en) * 2005-10-17 2007-04-26 Academia Sinica Pulmonary stem cells, related methods and kits
US8415153B2 (en) 2006-06-19 2013-04-09 Geron Corporation Differentiation and enrichment of islet-like cells from human pluripotent stem cells
CN102803472B (en) * 2009-06-25 2014-04-23 吉隆公司 Progeny cells differentiated from pluripotent stem cells with foreign phenotype removed
EP2852617B1 (en) * 2012-05-22 2018-12-12 Life Technologies AS Recombinant antibody compositions and methods of use thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1149898A2 (en) * 1993-12-23 2001-10-31 Infigen, Inc. Embryonic stem cells as nuclear donors and nuclear transfer techniques to produce chimeric and transgenic animals

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2230263A1 (en) * 1995-08-25 1997-03-06 Lifecell Corporation Reconstituted skin
US6875607B1 (en) * 1998-11-09 2005-04-05 Es Cell International Pte Ltd Embryonic stem cells
WO2001068815A1 (en) * 2000-03-14 2001-09-20 Es Cell International Pte Ltd Embryonic stem cells and neural progenitor cells derived therefrom
WO2001072970A2 (en) * 2000-03-28 2001-10-04 University Of Iowa Research Foundation Methods to prepare and use epidermal stem cells
EP2298867A3 (en) * 2000-06-20 2013-01-16 ES Cell International Pte Ltd. Method of controlling differentiation of embryonic stem (ES) cells by culturing ES cells in the presence of bmp-2 pathway antagonists

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1149898A2 (en) * 1993-12-23 2001-10-31 Infigen, Inc. Embryonic stem cells as nuclear donors and nuclear transfer techniques to produce chimeric and transgenic animals

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
KIRKLAND SUSAN C ET AL: "Collagen IV synthesis is restricted to the enteroendocrine pathway during multilineage differentiation of human colorectal epithelial stem cells", JOURNAL OF CELL SCIENCE, vol. 114, no. 11, June 2001 (2001-06-01), pages 2055 - 2064, XP002401763, ISSN: 0021-9533 *
KOIZUMI N ET AL: "Cultivated corneal epithelial stem cell transplantation in ocular surface disorders", OPHTHALMOLOGY, J. B. LIPPINCOTT CO., PHILADELPHIA, PA, US, vol. 108, no. 9, September 2001 (2001-09-01), pages 1569 - 1574, XP002988711, ISSN: 0161-6420 *
NIWA HITOSHI ET AL: "Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells", NATURE GENETICS, NATURE AMERICA, NEW YORK, US, vol. 24, no. 4, April 2000 (2000-04-01), pages 372 - 376, XP002155942, ISSN: 1061-4036 *
REUBINOFF BENJAMIN E ET AL: "Embryonic stem cell lines from human blastocysts: Somatic differentiation in vitro", NATURE BIOTECHNOLOGY, NATURE PUB. CO, NEW YORK, NY, US, vol. 18, no. 4, April 2000 (2000-04-01), pages 399 - 404, XP002195338, ISSN: 1087-0156 *
SCHULDINER MAYA ET AL: "Effects of eight growth factors on the differentiation of cells derived from human embryonic stem cells", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 97, no. 21, 10 October 2000 (2000-10-10), pages 11307 - 11312, XP002184277, ISSN: 0027-8424 *
See also references of WO03040355A1 *
THOMSON J A ET AL: "EMBRYONIC STEM CELL LINES DERIVED FROM HUMAN BLASTOCYSTS", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 282, 6 November 1998 (1998-11-06), pages 1145 - 1147, XP002121340, ISSN: 0036-8075 *

Also Published As

Publication number Publication date
GB2398079B (en) 2006-05-31
GB0412528D0 (en) 2004-07-07
GB2419890A (en) 2006-05-10
WO2003040355A1 (en) 2003-05-15
CA2466342A1 (en) 2003-05-15
GB0602441D0 (en) 2006-03-22
GB2398079A (en) 2004-08-11
EP1448763A1 (en) 2004-08-25

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