EP2510094A4 - COMPOSITIONS AND METHODS COMPRISING PROTEASE VARIANTS - Google Patents

COMPOSITIONS AND METHODS COMPRISING PROTEASE VARIANTS

Info

Publication number
EP2510094A4
EP2510094A4 EP10836666.7A EP10836666A EP2510094A4 EP 2510094 A4 EP2510094 A4 EP 2510094A4 EP 10836666 A EP10836666 A EP 10836666A EP 2510094 A4 EP2510094 A4 EP 2510094A4
Authority
EP
European Patent Office
Prior art keywords
compositions
methods
protease variants
protease
variants
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP10836666.7A
Other languages
German (de)
French (fr)
Other versions
EP2510094B1 (en
EP2510094A2 (en
Inventor
Joshua Roy Basler
Luis Gustavo Cascao-Pereira
David A Estell
James T Kellis
Alexander Pisarchik
Ayrookaran J Poulose
Daniel Torres-Pazmino
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Danisco US Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco US Inc filed Critical Danisco US Inc
Priority to EP19177621.0A priority Critical patent/EP3599279B1/en
Priority to DK16200028.5T priority patent/DK3190183T3/en
Priority to EP16200028.5A priority patent/EP3190183B1/en
Publication of EP2510094A2 publication Critical patent/EP2510094A2/en
Publication of EP2510094A4 publication Critical patent/EP2510094A4/en
Application granted granted Critical
Publication of EP2510094B1 publication Critical patent/EP2510094B1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cleaning By Liquid Or Steam (AREA)
  • Accessory Of Washing/Drying Machine, Commercial Washing/Drying Machine, Other Washing/Drying Machine (AREA)
  • Eyeglasses (AREA)
EP10836666.7A 2009-12-09 2010-12-09 Compositions and methods comprising protease variants Active EP2510094B1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP19177621.0A EP3599279B1 (en) 2009-12-09 2010-12-09 Compositions and methods comprising protease variants
DK16200028.5T DK3190183T3 (en) 2009-12-09 2010-12-09 COMPOSITIONS AND METHODS OF COMPREHENSIVE PROTEASE VARIETIES
EP16200028.5A EP3190183B1 (en) 2009-12-09 2010-12-09 Compositions and methods comprising protease variants

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28512709P 2009-12-09 2009-12-09
US39237310P 2010-10-12 2010-10-12
PCT/US2010/059628 WO2011072099A2 (en) 2009-12-09 2010-12-09 Compositions and methods comprising protease variants

Related Child Applications (2)

Application Number Title Priority Date Filing Date
EP16200028.5A Division EP3190183B1 (en) 2009-12-09 2010-12-09 Compositions and methods comprising protease variants
EP19177621.0A Division EP3599279B1 (en) 2009-12-09 2010-12-09 Compositions and methods comprising protease variants

Publications (3)

Publication Number Publication Date
EP2510094A2 EP2510094A2 (en) 2012-10-17
EP2510094A4 true EP2510094A4 (en) 2013-06-19
EP2510094B1 EP2510094B1 (en) 2016-11-30

Family

ID=44146173

Family Applications (3)

Application Number Title Priority Date Filing Date
EP16200028.5A Active EP3190183B1 (en) 2009-12-09 2010-12-09 Compositions and methods comprising protease variants
EP10836666.7A Active EP2510094B1 (en) 2009-12-09 2010-12-09 Compositions and methods comprising protease variants
EP19177621.0A Active EP3599279B1 (en) 2009-12-09 2010-12-09 Compositions and methods comprising protease variants

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EP16200028.5A Active EP3190183B1 (en) 2009-12-09 2010-12-09 Compositions and methods comprising protease variants

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP19177621.0A Active EP3599279B1 (en) 2009-12-09 2010-12-09 Compositions and methods comprising protease variants

Country Status (12)

Country Link
US (6) US8728790B2 (en)
EP (3) EP3190183B1 (en)
JP (4) JP5863666B2 (en)
CN (2) CN105368809A (en)
AR (1) AR079338A1 (en)
AU (1) AU2010328121A1 (en)
BR (1) BR112012014082B1 (en)
CA (1) CA2782891C (en)
DK (3) DK3599279T3 (en)
ES (1) ES2746931T3 (en)
MX (2) MX356282B (en)
WO (1) WO2011072099A2 (en)

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