EP2681317A4 - THERMOSTABLE VARIANTS OF FIBROBLAST GROWTH FACTORS - Google Patents

THERMOSTABLE VARIANTS OF FIBROBLAST GROWTH FACTORS

Info

Publication number: EP2681317A4
Authority: EP; European Patent Office
Prior art keywords: growth factors; fibroblast growth; thermostable variants; thermostable; variants
Prior art date: 2011-03-01
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Granted

Application number

EP12786156.5A

Other languages

German (de)

French (fr)

Other versions

EP2681317B1 (en

EP2681317A2 (en

Inventor

Soon Seog Jeong

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

HUMANZYME Inc

Original Assignee

HUMANZYME Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2011-03-01

Filing date

2012-03-01

Publication date

2014-11-12

2012-03-01 Application filed by HUMANZYME Inc filed Critical HUMANZYME Inc

2014-01-08 Publication of EP2681317A2 publication Critical patent/EP2681317A2/en

2014-11-12 Publication of EP2681317A4 publication Critical patent/EP2681317A4/en

2018-05-02 Application granted granted Critical

2018-05-02 Publication of EP2681317B1 publication Critical patent/EP2681317B1/en

Status Not-in-force legal-status Critical Current

2032-03-01 Anticipated expiration legal-status Critical

Links

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/50—Fibroblast growth factor [FGF]
- C07K14/503—Fibroblast growth factor [FGF] basic FGF [bFGF]
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Zoology (AREA)
Biomedical Technology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Biotechnology (AREA)
Gynecology & Obstetrics (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Developmental Biology & Embryology (AREA)
Reproductive Health (AREA)
Toxicology (AREA)
Gastroenterology & Hepatology (AREA)
Biophysics (AREA)
Medicinal Chemistry (AREA)
Molecular Biology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
General Engineering & Computer Science (AREA)
Cell Biology (AREA)
Microbiology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Peptides Or Proteins (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

EP12786156.5A 2011-03-01 2012-03-01 Thermostable variants of fibroblast growth factors Not-in-force EP2681317B1 (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US201161448107P	2011-03-01	2011-03-01
PCT/US2012/027310 WO2012158244A2 (en)	2011-03-01	2012-03-01	Thermostable variants of fibroblast growth factors

Publications (3)

Publication Number	Publication Date
EP2681317A2 EP2681317A2 (en)	2014-01-08
EP2681317A4 true EP2681317A4 (en)	2014-11-12
EP2681317B1 EP2681317B1 (en)	2018-05-02

Family

ID=46753566

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP12786156.5A Not-in-force EP2681317B1 (en)	2011-03-01	2012-03-01	Thermostable variants of fibroblast growth factors

Country Status (6)

Country	Link
US (1)	US9169309B2 (en)
EP (1)	EP2681317B1 (en)
JP (2)	JP6110318B2 (en)
CN (2)	CN103597076B (en)
CA (1)	CA2828874C (en)
WO (1)	WO2012158244A2 (en)

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WO2011130729A2 (en)	2010-04-16	2011-10-20	Salk Institute For Biological Studies	Methods for treating metabolic disorders using fgf
US9169309B2 (en) *	2011-03-01	2015-10-27	Humanzyme Inc.	Thermostable variants of fibroblast growth factors
WO2013090919A1 (en) *	2011-12-16	2013-06-20	Wisconsin Alumni Research Foundation	Fgf-2 having enhanced stability
WO2015061331A1 (en)	2013-10-21	2015-04-30	Salk Institute For Biological Studies	Chimeric fibroblast growth factor (fgf) 2/fgf1 peptides and methods of use
EP2930181B1 (en) *	2014-04-07	2017-10-11	Miltenyi Biotec GmbH	Fibroblast growth factor muteins with increased activity
GB2527286A (en) *	2014-06-11	2015-12-23	Rsr Ltd	Glycoprotein hormone receptor mutations
RU2558294C1 (en) *	2014-09-16	2015-07-27	Общество с ограниченной ответственностью "НекстГен"	Codon-optimised recombinant plasmid, stimulation technique for peripheral nerve regeneration, treatment modality for damaged human nerve
PL3380508T3 (en)	2015-11-27	2021-07-12	Masarykova Univerzita	Thermostable fgf2 polypeptide, use thereof
WO2017127493A1 (en) *	2016-01-22	2017-07-27	Salk Institute For Biological Studies	Fgf2 truncations and mutants and uses thereof
CN106957359B (en) *	2016-08-31	2020-04-24	黄志锋	FGF1 mutant and medical application thereof
US11267855B2 (en) *	2018-03-16	2022-03-08	The Board Of Trustees Of The University Of Arkansas	Engineered FGF1 and FGF2 compositions and methods of use thereof
CN111886334A (en) *	2018-03-20	2020-11-03	富士胶片株式会社	Method for three-dimensional culture of pluripotent stem cells
US12397084B2 (en)	2018-07-23	2025-08-26	Georgia Tech Research Corporation	Synthetic hydrogel carriers for muscle repair
CN114746436B (en)	2019-11-25	2024-07-02	韩国海洋科学技术院	FGF2 polypeptides with improved temperature stability and protease resistance and uses thereof
CN117242174A (en) *	2021-03-10	2023-12-15	谱系细胞疗法公司	Methods of generating oligodendrocyte progenitor cells and uses thereof
GB2616475A (en) *	2022-03-11	2023-09-13	Multus Biotechnology Ltd	Engineered fibroblast growth factor variants
CN114805534B (en) *	2022-04-14	2023-11-17	北京理工大学	Human fibroblast growth factor mutant and encoding gene, preparation method and application thereof
CN116041478A (en) *	2022-12-19	2023-05-02	太东(镇江)生物科技有限公司	Preparation method of human recombinant thermostable protein FGF2 based on Escherichia coli in vitro purification system
KR20240173282A (en) *	2023-06-02	2024-12-11	(주)피앤피바이오팜	Method for Preparing Stem Cell-derived Exosome using a Thermostable and long-acting Basic Fibroblast Growth Factor mutant
KR20240173283A (en) *	2023-06-02	2024-12-11	(주)피앤피바이오팜	Method for Culturing Stem Cells using a Thermostable and Long-acting Basic Fibroblast Growth Factor mutant
CN119192326B (en) *	2024-10-23	2025-06-17	四川大学	Recombinant cFGF2 protein and avian primordial germ cell culture medium and application thereof

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US5859208A (en) *	1988-07-06	1999-01-12	Fiddes; John C.	Human basic fibroblast growth factor analog
WO2003094835A2 (en) *	2002-05-09	2003-11-20	Prochon Biotech Ltd.	Fgf variants and methods for use thereof
WO2010083051A2 (en) *	2009-01-15	2010-07-22	ProChon Biotech, Ltd.	Cartilage particle tissue mixtures optionally combined with a cancellous construct

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DE69119869T2 (en) *	1990-11-23	1996-12-12	American Cyanamid Co	Chimeric fibroblast growth factor
CA2281925A1 (en) *	1997-02-26	1998-09-03	Ciblex Corporation	Inhibitors of leaderless protein export
EP1444995A1 (en) *	2003-02-06	2004-08-11	Institut National De La Sante Et De La Recherche Medicale (Inserm)	FGF-2 derived proteins for the preparation of biomaterials or medical devices such as stents
US20090111748A1 (en)	2005-07-21	2009-04-30	Ellerby Lisa M	Fibroblast growth factor-2 promotes neurogenesis and neuroprotection and prolongs survival in huntington's disease
US20100022453A1 (en)	2006-08-30	2010-01-28	University Of Rochester	NoGo Receptor 1 and Fibroblast Growth Factor Interactions
EP2083846B1 (en)	2006-09-28	2015-07-15	Hepacore Ltd.	N-terminal fgf variants having increased receptor selectivity and uses thereof
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US9169309B2 (en) *	2011-03-01	2015-10-27	Humanzyme Inc.	Thermostable variants of fibroblast growth factors

2012
- 2012-02-29 US US13/408,874 patent/US9169309B2/en active Active
- 2012-03-01 CA CA2828874A patent/CA2828874C/en active Active
- 2012-03-01 WO PCT/US2012/027310 patent/WO2012158244A2/en not_active Ceased
- 2012-03-01 JP JP2013556867A patent/JP6110318B2/en active Active
- 2012-03-01 EP EP12786156.5A patent/EP2681317B1/en not_active Not-in-force
- 2012-03-01 CN CN201280021174.5A patent/CN103597076B/en active Active
- 2012-03-01 CN CN201510815252.4A patent/CN105567700B/en active Active
2017
- 2017-03-09 JP JP2017044442A patent/JP2017140032A/en active Pending

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5859208A (en) *	1988-07-06	1999-01-12	Fiddes; John C.	Human basic fibroblast growth factor analog
WO2003094835A2 (en) *	2002-05-09	2003-11-20	Prochon Biotech Ltd.	Fgf variants and methods for use thereof
WO2010083051A2 (en) *	2009-01-15	2010-07-22	ProChon Biotech, Ltd.	Cartilage particle tissue mixtures optionally combined with a cancellous construct

Non-Patent Citations (3)

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Title
LEVENSTEIN MARK E ET AL: "Basic fibroblast growth factor support of human embryonic stem cell self-renewal", STEM CELLS, ALPHAMED PRESS, DAYTON, OH, US, vol. 24, no. 3, 1 March 2006 (2006-03-01), pages 568 - 574, XP009133580, ISSN: 1066-5099, [retrieved on 20051110], DOI: 10.1634/STEMCELLS.2005-0247 *
M. ZAKRZEWSKA ET AL: "Design of fully active FGF-1 variants with increased stability", PROTEIN ENGINEERING DESIGN AND SELECTION, vol. 17, no. 8, 23 September 2004 (2004-09-23), pages 603 - 611, XP055143455, ISSN: 1741-0126, DOI: 10.1093/protein/gzh076 *
ZAKRZEWSKA ET AL: "Highly Stable Mutants of Human Fibroblast Growth Factor-1 Exhibit Prolonged Biological Action", JOURNAL OF MOLECULAR BIOLOGY, ACADEMIC PRESS, UNITED KINGDOM, vol. 352, no. 4, 30 September 2005 (2005-09-30), pages 860 - 875, XP005067406, ISSN: 0022-2836, DOI: 10.1016/J.JMB.2005.07.066 *

Also Published As

Publication number	Publication date
WO2012158244A3 (en)	2013-01-24
WO2012158244A2 (en)	2012-11-22
US20120225479A1 (en)	2012-09-06
US9169309B2 (en)	2015-10-27
JP6110318B2 (en)	2017-04-05
EP2681317B1 (en)	2018-05-02
CA2828874A1 (en)	2012-11-22
EP2681317A2 (en)	2014-01-08
JP2014507951A (en)	2014-04-03
CN105567700B (en)	2019-06-25
JP2017140032A (en)	2017-08-17
CN103597076B (en)	2016-01-20
CA2828874C (en)	2019-01-08
CN105567700A (en)	2016-05-11
CN103597076A (en)	2014-02-19

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Publication	Publication Date	Title
EP2681317A4 (en)	2014-11-12	THERMOSTABLE VARIANTS OF FIBROBLAST GROWTH FACTORS
CO6910165A2 (en)	2014-03-31	Variants of fibroblast growth factor 21
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