EP3060648A4 - Systems, devices and methods for microfluidic culturing, manipulation and analysis of tissues and cells - Google Patents

Systems, devices and methods for microfluidic culturing, manipulation and analysis of tissues and cells Download PDF

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Publication number
EP3060648A4
EP3060648A4 EP14855429.8A EP14855429A EP3060648A4 EP 3060648 A4 EP3060648 A4 EP 3060648A4 EP 14855429 A EP14855429 A EP 14855429A EP 3060648 A4 EP3060648 A4 EP 3060648A4
Authority
EP
European Patent Office
Prior art keywords
manipulation
tissues
cells
analysis
systems
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP14855429.8A
Other languages
German (de)
French (fr)
Other versions
EP3060648A1 (en
Inventor
Ashok C. Chander
Wendell R. SU
Thiagarajan MEYYAPPAN
Jonathan VARSANIK
Senthil Ramu
Michael MANAK
Brad HOGAN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cellanyx Diagnostics LLC
Original Assignee
Cellanyx Diagnostics LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellanyx Diagnostics LLC filed Critical Cellanyx Diagnostics LLC
Publication of EP3060648A1 publication Critical patent/EP3060648A1/en
Publication of EP3060648A4 publication Critical patent/EP3060648A4/en
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP14855429.8A 2013-10-22 2014-10-22 Systems, devices and methods for microfluidic culturing, manipulation and analysis of tissues and cells Withdrawn EP3060648A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361894298P 2013-10-22 2013-10-22
PCT/US2014/061782 WO2015061458A1 (en) 2013-10-22 2014-10-22 Systems, devices and methods for microfluidic culturing, manipulation and analysis of tissues and cells

Publications (2)

Publication Number Publication Date
EP3060648A1 EP3060648A1 (en) 2016-08-31
EP3060648A4 true EP3060648A4 (en) 2017-05-31

Family

ID=52993507

Family Applications (1)

Application Number Title Priority Date Filing Date
EP14855429.8A Withdrawn EP3060648A4 (en) 2013-10-22 2014-10-22 Systems, devices and methods for microfluidic culturing, manipulation and analysis of tissues and cells

Country Status (3)

Country Link
EP (1) EP3060648A4 (en)
CA (1) CA2927947A1 (en)
WO (1) WO2015061458A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT2768942T (en) 2011-10-17 2020-01-21 Massachusetts Inst Technology Intracellular delivery
KR102243597B1 (en) 2013-08-16 2021-04-26 메사추세츠 인스티튜트 오브 테크놀로지 Selective delivery of material to cells
KR20170074235A (en) 2014-10-31 2017-06-29 메사추세츠 인스티튜트 오브 테크놀로지 Delivery of biomolecules to immune cells
CN107002089B (en) 2014-11-14 2021-09-07 麻省理工学院 Destruction and Field-Achieved Delivery of Compounds and Compositions into Cells
CN107250373A (en) 2015-01-12 2017-10-13 麻省理工学院 Gene editing via microfluidic delivery
AU2016289530B2 (en) 2015-07-09 2021-05-06 Massachusetts Institute Of Technology Delivery of materials to anucleate cells
US20180327706A1 (en) * 2015-10-19 2018-11-15 The Methodist Hospital Crispr-cas9 delivery to hard-to-transfect cells via membrane deformation
WO2017197343A2 (en) * 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
EP3556845A1 (en) * 2018-04-20 2019-10-23 Cellix Limited A method and device for transfecting cells
US20230221299A1 (en) * 2020-04-22 2023-07-13 Agency For Science, Technology And Research A device for studying interactions of a first cell type with a second cell type and related method
CN113522383B (en) * 2021-06-25 2023-02-24 东南大学 Cell working condition device

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011014674A2 (en) * 2009-07-29 2011-02-03 Cornell University Microfluidic device for pharmacokinetic-pharmacodynamic study of drugs and uses thereof
US20110136162A1 (en) * 2009-08-31 2011-06-09 Drexel University Compositions and Methods for Functionalized Patterning of Tissue Engineering Substrates Including Bioprinting Cell-Laden Constructs for Multicompartment Tissue Chambers
WO2012048269A2 (en) * 2010-10-08 2012-04-12 Chander Ashok C Systems, methods and devices for measuring growth/oncogenic & migration/metastatic potential
US20130143230A1 (en) * 2011-12-02 2013-06-06 The Trustees Of The Stevens Institute Of Technology Microfluidic-based cell-culturing platform and method
WO2013082612A1 (en) * 2011-12-03 2013-06-06 Emd Millipore Corporation Micro-incubation systems for microfluidic cell culture and methods
US20130224853A1 (en) * 2008-12-11 2013-08-29 Joule Unlimited Technologies, Inc. Solar Biofactory, Photobioreactors, Passive Thermal Regulation Systems And Methods for Producing Products

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130224853A1 (en) * 2008-12-11 2013-08-29 Joule Unlimited Technologies, Inc. Solar Biofactory, Photobioreactors, Passive Thermal Regulation Systems And Methods for Producing Products
WO2011014674A2 (en) * 2009-07-29 2011-02-03 Cornell University Microfluidic device for pharmacokinetic-pharmacodynamic study of drugs and uses thereof
US20110136162A1 (en) * 2009-08-31 2011-06-09 Drexel University Compositions and Methods for Functionalized Patterning of Tissue Engineering Substrates Including Bioprinting Cell-Laden Constructs for Multicompartment Tissue Chambers
WO2012048269A2 (en) * 2010-10-08 2012-04-12 Chander Ashok C Systems, methods and devices for measuring growth/oncogenic & migration/metastatic potential
US20130143230A1 (en) * 2011-12-02 2013-06-06 The Trustees Of The Stevens Institute Of Technology Microfluidic-based cell-culturing platform and method
WO2013082612A1 (en) * 2011-12-03 2013-06-06 Emd Millipore Corporation Micro-incubation systems for microfluidic cell culture and methods

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2015061458A1 *

Also Published As

Publication number Publication date
CA2927947A1 (en) 2015-04-30
EP3060648A1 (en) 2016-08-31
WO2015061458A1 (en) 2015-04-30

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Effective date: 20170428

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Ipc: C12M 3/04 20060101ALI20170421BHEP

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