EP3421596A4 - Alpha amylase variant and use thereof - Google Patents

Alpha amylase variant and use thereof Download PDF

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Publication number
EP3421596A4
EP3421596A4 EP17755837.6A EP17755837A EP3421596A4 EP 3421596 A4 EP3421596 A4 EP 3421596A4 EP 17755837 A EP17755837 A EP 17755837A EP 3421596 A4 EP3421596 A4 EP 3421596A4
Authority
EP
European Patent Office
Prior art keywords
alpha amylase
amylase variant
variant
alpha
amylase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP17755837.6A
Other languages
German (de)
French (fr)
Other versions
EP3421596A1 (en
Inventor
Yan Fan
Xiuzhen DU
Minghui Hao
Yan Sun
Ke Huang
Feng Li
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanjing Bestzyme Bioengineering Co Ltd
Original Assignee
Nanjing Bestzyme Bioengineering Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanjing Bestzyme Bioengineering Co Ltd filed Critical Nanjing Bestzyme Bioengineering Co Ltd
Publication of EP3421596A1 publication Critical patent/EP3421596A1/en
Publication of EP3421596A4 publication Critical patent/EP3421596A4/en
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/10—Bacillus licheniformis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
EP17755837.6A 2016-02-26 2017-02-24 Alpha amylase variant and use thereof Withdrawn EP3421596A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201610108232 2016-02-26
PCT/CN2017/074724 WO2017144008A1 (en) 2016-02-26 2017-02-24 Αlpha amylase variant and use thereof

Publications (2)

Publication Number Publication Date
EP3421596A1 EP3421596A1 (en) 2019-01-02
EP3421596A4 true EP3421596A4 (en) 2019-10-23

Family

ID=59684743

Family Applications (1)

Application Number Title Priority Date Filing Date
EP17755837.6A Withdrawn EP3421596A4 (en) 2016-02-26 2017-02-24 Alpha amylase variant and use thereof

Country Status (6)

Country Link
US (1) US11104909B2 (en)
EP (1) EP3421596A4 (en)
JP (1) JP2019506186A (en)
CN (1) CN108699544B (en)
AU (1) AU2017222025A1 (en)
WO (1) WO2017144008A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108699544B (en) * 2016-02-26 2021-10-26 南京百斯杰生物工程有限公司 Alpha-amylase variants and uses thereof
CN119630280A (en) 2022-05-14 2025-03-14 诺维信公司 Compositions and methods for preventing, treating, inhibiting and/or eliminating plant pathogenic infestations and infections

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996023873A1 (en) * 1995-02-03 1996-08-08 Novo Nordisk A/S Amylase variants
WO2007113292A2 (en) * 2006-04-04 2007-10-11 Novozymes A/S Mashing process
WO2009100138A2 (en) * 2008-02-04 2009-08-13 Danisco Us Inc., Genencor Division Variants of bacillus stearothermophilus alpha-amylase and uses thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005111203A2 (en) * 2004-04-08 2005-11-24 Genencor International, Inc. MUTANT α ΑMYLASES
EP2215201A2 (en) * 2007-11-05 2010-08-11 Danisco US Inc. Alpha-amylase variants with altered properties
CN102057040A (en) * 2008-06-06 2011-05-11 丹尼斯科美国公司 Geobacillus stearothermophilus alpha-amylase (AMYS) variants with improved properties
DK2414514T3 (en) * 2009-04-01 2015-08-24 Danisco Us Inc FORMATIONS AND PROCEDURES COMPREHENSIVE Alpha- amylase variants WITH CHANGED PROPERTIES
BR112014000143B1 (en) * 2011-07-06 2021-04-06 Novozymes A/S ALPHA-AMYLASE VARIANT, INSULATED POLYNUCLEOTIDE, EXPRESSION VECTOR, HOST CELL, METHODS OF PRODUCING A VARIANT OF ALPHA-AMYLASE, TO PRODUCE A FERMENTATION PRODUCT FROM A MATERIAL CONTAINING STARCH, AND, FOR THE BREEDING OF A DERIVED PRODUCT MODIFIED
CN104379737B (en) * 2012-06-08 2018-10-23 丹尼斯科美国公司 There is the active variant alpha amylase of enhancing to starch polymer
CN108699544B (en) * 2016-02-26 2021-10-26 南京百斯杰生物工程有限公司 Alpha-amylase variants and uses thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996023873A1 (en) * 1995-02-03 1996-08-08 Novo Nordisk A/S Amylase variants
WO2007113292A2 (en) * 2006-04-04 2007-10-11 Novozymes A/S Mashing process
WO2009100138A2 (en) * 2008-02-04 2009-08-13 Danisco Us Inc., Genencor Division Variants of bacillus stearothermophilus alpha-amylase and uses thereof

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
DECLERCK N ET AL: "Probing structural determinants specifying high thermostability in Bacillus licheniformis alpha-amylase", JOURNAL OF MOLECULAR BIOLOGY, ACADEMIC PRESS, UNITED KINGDOM, vol. 301, no. 4, 25 August 2000 (2000-08-25), pages 1041 - 1057, XP026826629, ISSN: 0022-2836, [retrieved on 20000825], DOI: 10.1006/JMBI.2000.4025 *
KAZUAKI IGARASHI ET AL: "IMPROVED THERMOSTABILITY OF A BACILLUS ALPHA-AMYLASE BY DELETION OFAN ARGININE-GLYCINE RESIDUE IS CAUSED BY ENHANCED CALCIUM BINDING", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ELSEVIER, AMSTERDAM, NL, vol. 248, no. 2, 1 January 1998 (1998-01-01), pages 372 - 377, XP002901159, ISSN: 0006-291X, DOI: 10.1006/BBRC.1998.8970 *
LI ZHU ET AL: "Improving the thermostability and enhancing the Ca2+binding of the maltohexaose-forming [alpha]-amylase from Bacillus stearothermophilus", JOURNAL OF BIOTECHNOLOGY, ELSEVIER, AMSTERDAM, NL, vol. 222, 8 February 2016 (2016-02-08), pages 65 - 72, XP029453248, ISSN: 0168-1656, DOI: 10.1016/J.JBIOTEC.2016.02.013 *
LIN L L ET AL: "Impact of Arg210-Ser211 deletion on thermostability of a truncated Bacillus sp. strain TS-23 alpha-amylase", PROCESS BIOCHEMISTRY, ELSEVIER LTD, GB, vol. 43, no. 5, 1 May 2008 (2008-05-01), pages 559 - 565, XP022588871, ISSN: 1359-5113, [retrieved on 20080206], DOI: 10.1016/J.PROCBIO.2008.01.020 *
See also references of WO2017144008A1 *
VIHINEN M ET AL: "C-terminal truncations of a thermostable Bacillus stearothermophilus alpha-amylase", MOLECULAR AND CELLULAR BIOLOGY,, vol. 7, no. 10, 1 January 1994 (1994-01-01), pages 1255 - 1259, XP009102864, ISSN: 0270-7306 *
YI-HAN LIU ET AL: "Acid stabilization of Bacillus licheniformis alpha amylase through introduction of mutations", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 80, no. 5, 15 July 2008 (2008-07-15), pages 795 - 803, XP019654085, ISSN: 1432-0614, DOI: 10.1007/S00253-008-1580-5 *

Also Published As

Publication number Publication date
EP3421596A1 (en) 2019-01-02
JP2019506186A (en) 2019-03-07
AU2017222025A1 (en) 2018-10-11
CN108699544A (en) 2018-10-23
US11104909B2 (en) 2021-08-31
CN108699544B (en) 2021-10-26
WO2017144008A1 (en) 2017-08-31
US20190062764A1 (en) 2019-02-28

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