EP3485043A4 - Séquençage de produits de transcription unicellulaires - Google Patents
Séquençage de produits de transcription unicellulaires Download PDFInfo
- Publication number
- EP3485043A4 EP3485043A4 EP17828411.3A EP17828411A EP3485043A4 EP 3485043 A4 EP3485043 A4 EP 3485043A4 EP 17828411 A EP17828411 A EP 17828411A EP 3485043 A4 EP3485043 A4 EP 3485043A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- single cell
- cell transcript
- transcript sequencing
- sequencing
- transcript
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662362425P | 2016-07-14 | 2016-07-14 | |
| US201762463487P | 2017-02-24 | 2017-02-24 | |
| PCT/US2017/041770 WO2018013723A1 (fr) | 2016-07-14 | 2017-07-12 | Séquençage de produits de transcription unicellulaires |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP3485043A1 EP3485043A1 (fr) | 2019-05-22 |
| EP3485043A4 true EP3485043A4 (fr) | 2020-03-25 |
Family
ID=60951893
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP17828411.3A Withdrawn EP3485043A4 (fr) | 2016-07-14 | 2017-07-12 | Séquençage de produits de transcription unicellulaires |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP3485043A4 (fr) |
| CN (1) | CN109661474A (fr) |
| CA (1) | CA3027423A1 (fr) |
| SG (1) | SG11201811048UA (fr) |
| WO (1) | WO2018013723A1 (fr) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101829182B1 (ko) | 2009-04-02 | 2018-03-29 | 플루이다임 코포레이션 | 표적 핵산의 바코딩을 위한 멀티 프라이머 증폭 방법 |
| SG10201605049QA (en) | 2011-05-20 | 2016-07-28 | Fluidigm Corp | Nucleic acid encoding reactions |
| US9404864B2 (en) | 2013-03-13 | 2016-08-02 | Denovo Sciences, Inc. | System for imaging captured cells |
| US10466160B2 (en) | 2011-08-01 | 2019-11-05 | Celsee Diagnostics, Inc. | System and method for retrieving and analyzing particles |
| EP2739587B1 (fr) | 2011-08-01 | 2020-05-27 | Denovo Sciences | Système de capture de cellules |
| US9752181B2 (en) | 2013-01-26 | 2017-09-05 | Denovo Sciences, Inc. | System and method for capturing and analyzing cells |
| US9707562B2 (en) | 2013-03-13 | 2017-07-18 | Denovo Sciences, Inc. | System for capturing and analyzing cells |
| US9856535B2 (en) | 2013-05-31 | 2018-01-02 | Denovo Sciences, Inc. | System for isolating cells |
| US10391490B2 (en) | 2013-05-31 | 2019-08-27 | Celsee Diagnostics, Inc. | System and method for isolating and analyzing cells |
| WO2017106777A1 (fr) | 2015-12-16 | 2017-06-22 | Fluidigm Corporation | Amplification multiplex de haut niveau |
| WO2019046307A1 (fr) | 2017-08-29 | 2019-03-07 | Celsee Diagnostics, Inc. | Système et procédé pour isoler et analyser des cellules |
| WO2020186094A1 (fr) * | 2019-03-12 | 2020-09-17 | Universal Sequencing Technology | Procédés de capture intracellulaire d'une seule cellule et ses applications |
| US10633693B1 (en) | 2019-04-16 | 2020-04-28 | Celsee Diagnostics, Inc. | System and method for leakage control in a particle capture system |
| EP3966307B1 (fr) | 2019-05-07 | 2024-11-20 | Bio-Rad Laboratories, Inc. | Système et procédé de traitement automatique de cellules individuelles |
| US11273439B2 (en) | 2019-05-07 | 2022-03-15 | Bio-Rad Laboratories, Inc. | System and method for target material retrieval from microwells |
| SE543469C2 (en) * | 2019-06-03 | 2021-03-02 | Di Wu | Method for creating a cDNA sequencing library |
| SG11202112898WA (en) | 2019-06-14 | 2021-12-30 | Bio Rad Laboratories | System and method for automated single cell processing and analyses |
| EP4013863A4 (fr) * | 2019-08-14 | 2023-08-30 | iRepertoire, Inc. | Procédé de capture de sonde pour la récupération de vdj de chaîne bêta de tcr alpha et bêta à partir d'arn transcrit inverse d'oligo-dt |
| US20210270703A1 (en) * | 2020-02-28 | 2021-09-02 | 10X Genomics, Inc. | Method for isolating nuclei and cells from tissues |
| US11504719B2 (en) | 2020-03-12 | 2022-11-22 | Bio-Rad Laboratories, Inc. | System and method for receiving and delivering a fluid for sample processing |
| EP4133102A1 (fr) * | 2020-04-07 | 2023-02-15 | Vaccim | Dispositif et procédé pour déterminer, au départ d'un échantillon préalablement prélevé chez un individu, qu'au moins un anticorps cible a été ou est présent chez ledit individu |
| KR20230021697A (ko) * | 2020-06-09 | 2023-02-14 | 심센 다이아그노스틱스 에이비 | 림프구 클론성 결정 |
| CN120041546A (zh) * | 2020-12-07 | 2025-05-27 | 中国科学院脑科学与智能技术卓越创新中心 | 一种单细胞转录组测序的方法 |
| WO2023107539A1 (fr) | 2021-12-10 | 2023-06-15 | Bio-Rad Laboratories, Inc. | Compositions, procédés et systèmes pour le traitement d'échantillons avec des particules fonctionnalisées à morphologie ajustable |
| CN114934096A (zh) * | 2022-05-31 | 2022-08-23 | 上海市生物医药技术研究院 | 用于实施免疫组库测序的组合物及试剂盒和测序方法 |
| GB2629157A (en) * | 2023-04-18 | 2024-10-23 | The Institute Of Cancer Res Royal Cancer Hospital | T cell receptor sequencing |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060040283A1 (en) * | 2001-04-11 | 2006-02-23 | The Gov. Of The Usa As Represented By The Secretary Of The Dept. Of Health & Human Services | Methods of manipulating nucleic acids |
| US20140308669A1 (en) * | 2011-01-24 | 2014-10-16 | Epic Science, Inc. | Methods for obtaining single cells and applications of single cell omics |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101829182B1 (ko) * | 2009-04-02 | 2018-03-29 | 플루이다임 코포레이션 | 표적 핵산의 바코딩을 위한 멀티 프라이머 증폭 방법 |
| SG11201408128WA (en) * | 2012-06-15 | 2015-01-29 | Adaptive Biotechnologies Corp | Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set |
| US9708654B2 (en) * | 2012-06-15 | 2017-07-18 | Board Of Regents, The University Of Texas System | High throughput sequencing of multiple transcripts |
| ES2895750T3 (es) * | 2014-09-15 | 2022-02-22 | Abvitro Llc | Secuenciación de alto rendimiento de colecciones de nucleótidos |
| US10993997B2 (en) * | 2014-12-19 | 2021-05-04 | The Broad Institute, Inc. | Methods for profiling the t cell repertoire |
-
2017
- 2017-07-12 CN CN201780044623.0A patent/CN109661474A/zh active Pending
- 2017-07-12 WO PCT/US2017/041770 patent/WO2018013723A1/fr not_active Ceased
- 2017-07-12 EP EP17828411.3A patent/EP3485043A4/fr not_active Withdrawn
- 2017-07-12 SG SG11201811048UA patent/SG11201811048UA/en unknown
- 2017-07-12 CA CA3027423A patent/CA3027423A1/fr active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060040283A1 (en) * | 2001-04-11 | 2006-02-23 | The Gov. Of The Usa As Represented By The Secretary Of The Dept. Of Health & Human Services | Methods of manipulating nucleic acids |
| US20140308669A1 (en) * | 2011-01-24 | 2014-10-16 | Epic Science, Inc. | Methods for obtaining single cells and applications of single cell omics |
Non-Patent Citations (4)
| Title |
|---|
| ANTOINE-EMMANUEL SALIBA ET AL: "Single-cell RNA-seq: advances and future challenges", NUCLEIC ACIDS RESEARCH, vol. 42, no. 14, 22 July 2014 (2014-07-22), pages 8845 - 8860, XP055544044, ISSN: 0305-1048, DOI: 10.1093/nar/gku555 * |
| DAVID SVEC ET AL: "Direct Cell Lysis for Single-Cell Gene Expression Profiling", FRONTIERS IN ONCOLOGY, vol. 3, 1 January 2013 (2013-01-01), XP055167177, DOI: 10.3389/fonc.2013.00274 * |
| MOUSTAFA ATTAR ET AL: "A practical solution for preserving single cells for RNA sequencing", SCIENTIFIC REPORTS, vol. 8, no. 1, 1 February 2018 (2018-02-01), pages 2151, XP055640844, DOI: 10.1038/s41598-018-20372-7 * |
| See also references of WO2018013723A1 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CA3027423A1 (fr) | 2018-01-18 |
| SG11201811048UA (en) | 2019-01-30 |
| CN109661474A (zh) | 2019-04-19 |
| WO2018013723A1 (fr) | 2018-01-18 |
| WO2018013723A9 (fr) | 2019-03-28 |
| EP3485043A1 (fr) | 2019-05-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE |
|
| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
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| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE |
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| 17P | Request for examination filed |
Effective date: 20190213 |
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| AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
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| AX | Request for extension of the european patent |
Extension state: BA ME |
|
| DAV | Request for validation of the european patent (deleted) | ||
| DAX | Request for extension of the european patent (deleted) | ||
| RIC1 | Information provided on ipc code assigned before grant |
Ipc: C12Q 1/6806 20180101AFI20191115BHEP Ipc: C12N 15/10 20060101ALI20191115BHEP |
|
| A4 | Supplementary search report drawn up and despatched |
Effective date: 20200225 |
|
| RIC1 | Information provided on ipc code assigned before grant |
Ipc: C12Q 1/6806 20180101AFI20200219BHEP Ipc: C12N 15/10 20060101ALI20200219BHEP |
|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: EXAMINATION IS IN PROGRESS |
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| 17Q | First examination report despatched |
Effective date: 20201021 |
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| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
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| 18D | Application deemed to be withdrawn |
Effective date: 20210501 |