EP3519794A4 - Clearing agent and mounting media for microscopy - Google Patents

Clearing agent and mounting media for microscopy Download PDF

Info

Publication number
EP3519794A4
EP3519794A4 EP17857313.5A EP17857313A EP3519794A4 EP 3519794 A4 EP3519794 A4 EP 3519794A4 EP 17857313 A EP17857313 A EP 17857313A EP 3519794 A4 EP3519794 A4 EP 3519794A4
Authority
EP
European Patent Office
Prior art keywords
microscopy
clearing agent
mounting media
media
mounting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP17857313.5A
Other languages
German (de)
French (fr)
Other versions
EP3519794A1 (en
Inventor
James E. Simon
Thomas VILLANI
Adolfina KOROCH
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rutgers State University of New Jersey
Original Assignee
Rutgers State University of New Jersey
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US15/277,118 external-priority patent/US10018540B2/en
Application filed by Rutgers State University of New Jersey filed Critical Rutgers State University of New Jersey
Publication of EP3519794A1 publication Critical patent/EP3519794A1/en
Publication of EP3519794A4 publication Critical patent/EP3519794A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/33—Immersion oils, or microscope systems or objectives for use with immersion fluids

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Optics & Photonics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Microscoopes, Condenser (AREA)
EP17857313.5A 2016-09-27 2017-09-27 Clearing agent and mounting media for microscopy Withdrawn EP3519794A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15/277,118 US10018540B2 (en) 2012-04-10 2016-09-27 Clearing agent and mounting media for microscopy
PCT/US2017/053654 WO2018064132A1 (en) 2016-09-27 2017-09-27 Clearing agent and mounting media for microscopy

Publications (2)

Publication Number Publication Date
EP3519794A1 EP3519794A1 (en) 2019-08-07
EP3519794A4 true EP3519794A4 (en) 2020-05-20

Family

ID=61760981

Family Applications (1)

Application Number Title Priority Date Filing Date
EP17857313.5A Withdrawn EP3519794A4 (en) 2016-09-27 2017-09-27 Clearing agent and mounting media for microscopy

Country Status (3)

Country Link
EP (1) EP3519794A4 (en)
JP (1) JP2019536005A (en)
WO (1) WO2018064132A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW202227800A (en) * 2020-11-10 2022-07-16 日商住友化學股份有限公司 Method and kit for making biological material transparent
US20240167922A1 (en) * 2021-03-12 2024-05-23 Kyoto University Transparentizing reagent and transparentizing method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150147779A1 (en) * 2012-04-10 2015-05-28 Rutgers, The State University Of New Jersey Clearing Agent and Mounting Medium for Microscopy
US20160003715A1 (en) * 2014-07-03 2016-01-07 Richard Torres Novel Methods of Tissue Processing and Imaging

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102010013950A1 (en) * 2010-03-30 2011-10-06 Technische Universität Wien Histological procedure
CN103492852B (en) * 2011-04-28 2016-08-17 独立行政法人理化学研究所 The transparence method of biomaterial and utilization thereof
CN103207104B (en) * 2013-03-29 2015-12-23 中国科学院华南植物园 Ferro element colouring method in a kind of plant

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150147779A1 (en) * 2012-04-10 2015-05-28 Rutgers, The State University Of New Jersey Clearing Agent and Mounting Medium for Microscopy
US20160003715A1 (en) * 2014-07-03 2016-01-07 Richard Torres Novel Methods of Tissue Processing and Imaging

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANONYMOUS: "2,2,2-Trichloroethanol - Wikipedia", 14 December 2019 (2019-12-14), XP055684975, Retrieved from the Internet <URL:https://en.wikipedia.org/wiki/2,2,2-Trichloroethanol> [retrieved on 20200409] *
ANONYMOUS: "benzyl alcohol (YMDB01426) - Yeast Metabolome Database", 1 January 2015 (2015-01-01), XP055856777, Retrieved from the Internet <URL:http://www.ymdb.ca/compounds/YMDB01426> [retrieved on 20211101] *
KLAUS BECKER ET AL: "Chemical Clearing and Dehydration of GFP Expressing Mouse Brains", PLOS ONE, vol. 7, no. 3, 30 March 2012 (2012-03-30), pages e33916, XP055500434, DOI: 10.1371/journal.pone.0033916 *
See also references of WO2018064132A1 *

Also Published As

Publication number Publication date
WO2018064132A1 (en) 2018-04-05
JP2019536005A (en) 2019-12-12
EP3519794A1 (en) 2019-08-07

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