EP3853346A4 - USE OF CHLOROPLASTS FOR OXYGEN PRODUCTION IN CELL CULTURES - Google Patents

USE OF CHLOROPLASTS FOR OXYGEN PRODUCTION IN CELL CULTURES Download PDF

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Publication number
EP3853346A4
EP3853346A4 EP19861733.4A EP19861733A EP3853346A4 EP 3853346 A4 EP3853346 A4 EP 3853346A4 EP 19861733 A EP19861733 A EP 19861733A EP 3853346 A4 EP3853346 A4 EP 3853346A4
Authority
EP
European Patent Office
Prior art keywords
chloroplasts
cell cultures
oxygen production
oxygen
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP19861733.4A
Other languages
German (de)
French (fr)
Other versions
EP3853346A1 (en
Inventor
Glenn Gaudette
Tanja Dominko
Joshua GERSHLAK
Brent PERLMAN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Worcester Polytechnic Institute
Original Assignee
Worcester Polytechnic Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Worcester Polytechnic Institute filed Critical Worcester Polytechnic Institute
Publication of EP3853346A1 publication Critical patent/EP3853346A1/en
Publication of EP3853346A4 publication Critical patent/EP3853346A4/en
Withdrawn legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • C12N5/0663Bone marrow mesenchymal stem cells (BM-MSC)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/02Atmosphere, e.g. low oxygen conditions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70Enzymes
    • C12N2501/71Oxidoreductases (EC 1.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2529/00Culture process characterised by the use of electromagnetic stimulation
    • C12N2529/10Stimulation by light

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Rheumatology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP19861733.4A 2018-09-21 2019-09-20 USE OF CHLOROPLASTS FOR OXYGEN PRODUCTION IN CELL CULTURES Withdrawn EP3853346A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862734314P 2018-09-21 2018-09-21
PCT/US2019/052231 WO2020061500A1 (en) 2018-09-21 2019-09-20 Use of chloroplasts for oxygen production in cell cultures

Publications (2)

Publication Number Publication Date
EP3853346A1 EP3853346A1 (en) 2021-07-28
EP3853346A4 true EP3853346A4 (en) 2022-06-01

Family

ID=69888943

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19861733.4A Withdrawn EP3853346A4 (en) 2018-09-21 2019-09-20 USE OF CHLOROPLASTS FOR OXYGEN PRODUCTION IN CELL CULTURES

Country Status (3)

Country Link
EP (1) EP3853346A4 (en)
CA (1) CA3110233A1 (en)
WO (1) WO2020061500A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116585530B (en) * 2023-05-05 2024-02-02 暨南大学 An efficient oxygen-producing chloroplast composite hydrogel and its preparation method and application

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130089521A1 (en) * 2010-03-22 2013-04-11 Jose-Tomas Eagana-Erazo Use of photosynthetic scaffolds in tissue engineering

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016037010A1 (en) * 2014-09-03 2016-03-10 Symbiox, Inc. Photosynthetic cellular substances and methods of use thereof
JP6744665B2 (en) * 2015-06-25 2020-08-19 学校法人東京女子医科大学 Method for culturing animal cell composition, method for producing animal cell composition using the same, and animal cell composition

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130089521A1 (en) * 2010-03-22 2013-04-11 Jose-Tomas Eagana-Erazo Use of photosynthetic scaffolds in tissue engineering

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
IAN MICHAEL DIAMOND: "Worcester Polytechnic Institute Digital WPI Can Plant Chloroplasts Function in Mammalian Cells?", 1 January 2018 (2018-01-01), XP055694995, Retrieved from the Internet <URL:https://digitalcommons.wpi.edu/cgi/viewcontent.cgi?article=3187&context=mqp-all> *
NASS ET AL.: "Uptake of isolated chloroplasts by mammalian cells", SCIENCE, vol. 168, 12 September 1969 (1969-09-12), Weinheim, pages 1128 - 1131, XP055781994, Retrieved from the Internet <URL:https://www.science.org/doi/10.1126/science.165.3898.1128https://onlinelibrary.wiley.com/doi/10.1002/mnfr.201200058> [retrieved on 20210304], DOI: 10.1126/science.165.3898.1128 *
See also references of WO2020061500A1 *

Also Published As

Publication number Publication date
CA3110233A1 (en) 2020-03-26
EP3853346A1 (en) 2021-07-28
WO2020061500A1 (en) 2020-03-26

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