EP4006149A4 - MUTANT GLUCOSE OXIDASE (GOD) HAVING ENHANCED HEAT AND HEAT STABILITY, AND RELATED APPLICATION - Google Patents

MUTANT GLUCOSE OXIDASE (GOD) HAVING ENHANCED HEAT AND HEAT STABILITY, AND RELATED APPLICATION Download PDF

Info

Publication number
EP4006149A4
EP4006149A4 EP19939238.2A EP19939238A EP4006149A4 EP 4006149 A4 EP4006149 A4 EP 4006149A4 EP 19939238 A EP19939238 A EP 19939238A EP 4006149 A4 EP4006149 A4 EP 4006149A4
Authority
EP
European Patent Office
Prior art keywords
heat
god
related application
glucose oxidase
mutant glucose
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP19939238.2A
Other languages
German (de)
French (fr)
Other versions
EP4006149B1 (en
EP4006149C0 (en
EP4006149A1 (en
Inventor
Bin Yao
Tao Tu
Xiao Jiang
Huiying Luo
Xiaoyun Su
Huoqing Huang
Yaru Wang
Yingguo Bai
Yuan Wang
Xia SHI
Jie Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institute Of Animal Science Of Chinese Academy Ofagricultural Sciences
Original Assignee
Feed Research Institute of Chinese Academy of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Feed Research Institute of Chinese Academy of Agricultural Sciences filed Critical Feed Research Institute of Chinese Academy of Agricultural Sciences
Publication of EP4006149A1 publication Critical patent/EP4006149A1/en
Publication of EP4006149A4 publication Critical patent/EP4006149A4/en
Application granted granted Critical
Publication of EP4006149B1 publication Critical patent/EP4006149B1/en
Publication of EP4006149C0 publication Critical patent/EP4006149C0/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
    • C12Y101/03004—Glucose oxidase (1.1.3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/66—Aspergillus
    • C12R2001/685—Aspergillus niger
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/84—Pichia

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)
EP19939238.2A 2019-07-26 2019-11-13 Mutant glucose oxidase (god) having improved thermal stability and gene and application thereof Active EP4006149B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201910681815 2019-07-26
PCT/CN2019/117826 WO2021017292A1 (en) 2019-07-26 2019-11-13 Mutant glucose oxidase (god) having improved thermal stability and gene and application thereof

Publications (4)

Publication Number Publication Date
EP4006149A1 EP4006149A1 (en) 2022-06-01
EP4006149A4 true EP4006149A4 (en) 2023-05-31
EP4006149B1 EP4006149B1 (en) 2024-11-06
EP4006149C0 EP4006149C0 (en) 2024-11-06

Family

ID=74229020

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19939238.2A Active EP4006149B1 (en) 2019-07-26 2019-11-13 Mutant glucose oxidase (god) having improved thermal stability and gene and application thereof

Country Status (4)

Country Link
US (1) US12522806B2 (en)
EP (1) EP4006149B1 (en)
CN (1) CN112301009B (en)
WO (1) WO2021017292A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113528476B (en) * 2021-08-20 2023-01-31 福建福大百特生物科技有限公司 Glucose oxidase mutant and coding gene and efficient recombinant expression thereof
CN114181916B (en) * 2021-11-16 2023-03-14 华东师范大学 Artificially modified enzyme based on glucose oxidase and expression application thereof
CN113862237B (en) * 2021-12-02 2022-03-25 中国农业科学院北京畜牧兽医研究所 Methods and mutants, genes and applications for improving the thermostability of phytase
CN113862233B (en) * 2021-12-03 2022-03-25 中国农业科学院北京畜牧兽医研究所 Method for improving acid stability of glucose oxidase, mutant Q241E/R499E, gene and application
CN114369562B (en) * 2022-03-21 2022-05-31 中国农业科学院北京畜牧兽医研究所 Method for improving expression quantity of 5-aminolevulinic acid
CN114736881B (en) * 2022-06-09 2022-09-27 中国农业科学院北京畜牧兽医研究所 Glucose oxidase GoxM10 mutant A4D with improved acid stability and derivative mutant and application thereof
CN114736879B (en) * 2022-06-09 2022-09-27 中国农业科学院北京畜牧兽医研究所 Glucose oxidase GoxM10 mutant E361P with improved heat stability and derivative mutant and application thereof
CN114736880B (en) * 2022-06-09 2022-09-27 中国农业科学院北京畜牧兽医研究所 Mutant D497N of glucose oxidase GoxM10 with improved acid stability as well as derivative mutant and application thereof
CN118853615B (en) * 2024-09-23 2025-01-17 中国农业科学院北京畜牧兽医研究所 Glucose oxidase GoxM12 mutant G272S with improved thermal stability and its derivative mutants and applications
CN118853616B (en) * 2024-09-23 2025-02-21 中国农业科学院北京畜牧兽医研究所 Method for improving thermostability and acid stability of glucose oxidase, mutant GoxM, gene and application
CN118853613B (en) * 2024-09-23 2025-01-17 中国农业科学院北京畜牧兽医研究所 Glucose oxidase GoxM12 mutant G272T with improved thermal stability and its derivative mutants and applications

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014114810A1 (en) * 2013-01-28 2014-07-31 Roche Diagnostics Gmbh Novel glucose oxidases derived from aspergillus niger
CN108118036A (en) * 2016-11-28 2018-06-05 青岛蔚蓝生物集团有限公司 Novel grape carbohydrate oxidase mutant
CN108118038A (en) * 2016-11-28 2018-06-05 青岛蔚蓝生物集团有限公司 A kind of glucose oxidase mutant
CN108118037A (en) * 2016-11-28 2018-06-05 青岛蔚蓝生物集团有限公司 The glucose oxidase mutant that a kind of heat resistance improves
CN108251391A (en) * 2017-08-18 2018-07-06 青岛蔚蓝生物集团有限公司 Novel grape carbohydrate oxidase mutant
CN108251389A (en) * 2017-08-18 2018-07-06 青岛蔚蓝生物集团有限公司 The glucose oxidase mutant that a kind of heat resistance improves
CN108251392A (en) * 2018-03-30 2018-07-06 广东溢多利生物科技股份有限公司 It improves than living and the glucose oxidase mutant of thermal stability and its encoding gene and application
CN108893453A (en) * 2018-06-04 2018-11-27 中国农业科学院饲料研究所 Glucose oxidase GOD mutant and its gene and application

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5866406A (en) * 1990-02-02 1999-02-02 The Board Of Regents Of The University Of Nebraska Oxidase-producing aspergillus niger
CN101955953A (en) * 2010-09-09 2011-01-26 中国农业科学院生物技术研究所 Glucose oxidase mutant gene, expression and application thereof
CN103525778B (en) 2013-10-18 2015-07-01 江南大学 Glucose oxidase mutant with high catalytic activity
CN105950578A (en) 2016-07-06 2016-09-21 青岛红樱桃生物技术有限公司 Heat-resisting glucose oxidase mutant as well as encoding gene and application thereof
CN107189991B (en) 2017-05-08 2020-05-22 中国农业科学院饲料研究所 A kind of glucose oxidase mutant and its encoding gene and application
CN107012130B (en) * 2017-06-02 2020-05-22 中国农业科学院饲料研究所 Glucose oxidase mutant and coding gene and application thereof
CN108374001B (en) 2018-03-30 2021-02-26 广东溢多利生物科技股份有限公司 Glucose oxidase mutant capable of improving specific activity and coding gene and application thereof

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014114810A1 (en) * 2013-01-28 2014-07-31 Roche Diagnostics Gmbh Novel glucose oxidases derived from aspergillus niger
CN108118036A (en) * 2016-11-28 2018-06-05 青岛蔚蓝生物集团有限公司 Novel grape carbohydrate oxidase mutant
CN108118038A (en) * 2016-11-28 2018-06-05 青岛蔚蓝生物集团有限公司 A kind of glucose oxidase mutant
CN108118037A (en) * 2016-11-28 2018-06-05 青岛蔚蓝生物集团有限公司 The glucose oxidase mutant that a kind of heat resistance improves
CN108251391A (en) * 2017-08-18 2018-07-06 青岛蔚蓝生物集团有限公司 Novel grape carbohydrate oxidase mutant
CN108251389A (en) * 2017-08-18 2018-07-06 青岛蔚蓝生物集团有限公司 The glucose oxidase mutant that a kind of heat resistance improves
CN108251392A (en) * 2018-03-30 2018-07-06 广东溢多利生物科技股份有限公司 It improves than living and the glucose oxidase mutant of thermal stability and its encoding gene and application
CN108893453A (en) * 2018-06-04 2018-11-27 中国农业科学院饲料研究所 Glucose oxidase GOD mutant and its gene and application

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
JIANG XIAO ET AL: "Exploiting the activity-stability trade-off of glucose oxidase from Aspergillus niger using a simple approach to calculate thermostability of mutants", FOOD CHEMISTRY, ELSEVIER LTD, NL, vol. 342, 2 October 2020 (2020-10-02), XP086462186, ISSN: 0308-8146, [retrieved on 20201002], DOI: 10.1016/J.FOODCHEM.2020.128270 *
MU QINGXUAN ET AL: "Supplementary materials Thermostability Improvement of the Glucose Oxidase from Aspergillus niger for Efficient Gluconic Acid Production via Computational Design", INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 14 June 2019 (2019-06-14), pages 1 - 4, XP093038230, Retrieved from the Internet <URL:https://ars.els-cdn.com/content/image/1-s2.0-S014181301933750X-mmc1.docx> [retrieved on 20230411] *
MU QINGXUAN ET AL: "Thermostability improvement of the glucose oxidase from Aspergillus niger for efficient gluconic acid production via computational design", INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, ELSEVIER BV, NL, vol. 136, 14 June 2019 (2019-06-14), pages 1060 - 1068, XP085753762, ISSN: 0141-8130, [retrieved on 20190614], DOI: 10.1016/J.IJBIOMAC.2019.06.094 *
See also references of WO2021017292A1 *

Also Published As

Publication number Publication date
EP4006149B1 (en) 2024-11-06
US12522806B2 (en) 2026-01-13
EP4006149C0 (en) 2024-11-06
WO2021017292A9 (en) 2021-04-01
CN112301009A (en) 2021-02-02
CN112301009B (en) 2022-12-09
EP4006149A1 (en) 2022-06-01
WO2021017292A1 (en) 2021-02-04
US20230193215A1 (en) 2023-06-22

Similar Documents

Publication Publication Date Title
EP4006149A4 (en) MUTANT GLUCOSE OXIDASE (GOD) HAVING ENHANCED HEAT AND HEAT STABILITY, AND RELATED APPLICATION
EP3805378A4 (en) GLUCOSE OXIDASE (GOD) MUTANT AND RELATED GENE AND APPLICATION
EP3978602A4 (en) THERMOSTABLE GLUCOSE OXIDASE
EP4211234A4 (en) BASE-EDITING ENZYMES
LT3765615T (en) NEW TARGETING CRISPR DNA ENZYMES AND SYSTEMS
ATE518010T1 (en) ACTIVE SURFACE COUPLED POLYMERASES
EA201991455A1 (en) COMPOSITIONS AND METHODS FOR TREATING ALPHA-1-ANTITRIPSIN DEFICIENCY
DK3946884T3 (en) ENZYMATIC MULTI-LAYER ITEM
EP3882343A3 (en) Thermostable variants of t7 rna polymerase
DK3284816T3 (en) CULTIVATION MEDIA ADDITION, CULTURE MEDIA COMPOSITION AND METHOD OF CULTIVING CELLS OR TISSUE USING THE SAME
EP4426826A4 (en) BASE-EDITING ENZYMES
DK3272860T3 (en) Pyruvate dehydrogenase mutant, microorganism comprising the mutant, and method of producing L-amino acid using the microorganism
DK2344486T3 (en) Alkylthiazole carbamate derivatives, their preparation and their use as inhibitors of the enzyme FAAH
ZA202208798B (en) Anti-tigit antibodies, multispecific antibodies comprising the same and methods of using the same
DK3783102T3 (en) AMYLASE MUTANT HAVING HIGH SPECIFIC ACTIVITY AND THERMAL STABILITY, GENE OF THE MUTANT AND USES THEREOF
IL317515A (en) Engineered polymerases with improved thermal stability
IL284644A (en) Multispecific antibodies with specificity for TNFA and IL-17A, antibodies targeting IL-17A, and methods of using them
ZA202208799B (en) Anti-tigit antibodies, multispecific antibodies comprising the same and methods of using the same
EA200702430A1 (en) MODIFIED MICROORGANISMS WITH INACTIVATED LACTATE DEHYDROGENASE GENE
JP1727557S (en) Toaster oven
EP3647414A4 (en) GLUCOSE OXIDASE CNGODA, ASSOCIATED GENE AND APPLICATION
EP3661967A4 (en) RECOMBINANT GLUCOSE DEHYDROGENASE FLAVINE ADENINE DINUCLEOTIDE AND RELATED USES
EP4225896A4 (en) MANIPULATED ENZYMES WITH GALACTOSE OXIDASE VARIANTS
EP4265727A4 (en) MODIFIED GLUCOSE DEHYDROGENASE
EP4010467C0 (en) MUTANT PGLB OLIGOSACCHARYLTRANSFERASE ENZYMES

Legal Events

Date Code Title Description
STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE

PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE

17P Request for examination filed

Effective date: 20220223

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DAV Request for validation of the european patent (deleted)
DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20230503

RIC1 Information provided on ipc code assigned before grant

Ipc: C12R 1/84 20060101ALI20230425BHEP

Ipc: C12R 1/685 20060101ALI20230425BHEP

Ipc: C12N 15/81 20060101ALI20230425BHEP

Ipc: C12N 15/53 20060101ALI20230425BHEP

Ipc: C12N 9/04 20060101AFI20230425BHEP

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: GRANT OF PATENT IS INTENDED

INTG Intention to grant announced

Effective date: 20240603

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE PATENT HAS BEEN GRANTED

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: INSTITUTE OF ANIMAL SCIENCE OF CHINESE ACADEMY OFAGRICULTURAL SCIENCES

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 602019061775

Country of ref document: DE

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

U01 Request for unitary effect filed

Effective date: 20241106

U07 Unitary effect registered

Designated state(s): AT BE BG DE DK EE FI FR IT LT LU LV MT NL PT RO SE SI

Effective date: 20241120

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: HR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20241106

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250306

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: ES

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20241106

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: NO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250206

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250207

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: PL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20241106

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: RS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20250206

REG Reference to a national code

Ref country code: CH

Ref legal event code: PL

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SM

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20241106

U21 Renewal fee for the european patent with unitary effect paid with additional fee

Year of fee payment: 6

Effective date: 20250602

REG Reference to a national code

Ref country code: CH

Ref legal event code: PL

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CH

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20241130

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20241106

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20241106

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MC

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20241106

26N No opposition filed

Effective date: 20250807

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20241113

GBPC Gb: european patent ceased through non-payment of renewal fee

Effective date: 20250206

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GB

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20250206

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: HU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT; INVALID AB INITIO

Effective date: 20191113

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CY

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT; INVALID AB INITIO

Effective date: 20191113