EP4097449A4 - METHODS AND SYSTEMS FOR CLASSIFYING FLOW CYTOMETER DATA - Google Patents

METHODS AND SYSTEMS FOR CLASSIFYING FLOW CYTOMETER DATA Download PDF

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Publication number: EP4097449A4
Authority: EP; European Patent Office
Prior art keywords: systems; methods; flow cytometer; cytometer data; classifying flow
Prior art date: 2020-01-31
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Granted

Application number

EP21747161.4A

Other languages

German (de)

French (fr)

Other versions

EP4097449B1 (en

EP4097449A1 (en

Inventor

Richard Lee HALPERT

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Becton Dickinson and Co

Original Assignee

Becton Dickinson and Co

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2020-01-31

Filing date

2021-01-20

Publication date

2023-07-05

2021-01-20 Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co

2022-12-07 Publication of EP4097449A1 publication Critical patent/EP4097449A1/en

2023-07-05 Publication of EP4097449A4 publication Critical patent/EP4097449A4/en

2025-08-27 Application granted granted Critical

2025-08-27 Publication of EP4097449B1 publication Critical patent/EP4097449B1/en

Status Active legal-status Critical Current

2041-01-20 Anticipated expiration legal-status Critical

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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1429—Signal processing
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
- G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
- G—PHYSICS
- G06—COMPUTING OR CALCULATING; COUNTING
- G06F—ELECTRIC DIGITAL DATA PROCESSING
- G06F18/00—Pattern recognition
- G06F18/20—Analysing
- G06F18/23—Clustering techniques
- G—PHYSICS
- G06—COMPUTING OR CALCULATING; COUNTING
- G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
- G06V10/00—Arrangements for image or video recognition or understanding
- G06V10/70—Arrangements for image or video recognition or understanding using pattern recognition or machine learning
- G06V10/762—Arrangements for image or video recognition or understanding using pattern recognition or machine learning using clustering, e.g. of similar faces in social networks
- G—PHYSICS
- G06—COMPUTING OR CALCULATING; COUNTING
- G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
- G06V10/00—Arrangements for image or video recognition or understanding
- G06V10/70—Arrangements for image or video recognition or understanding using pattern recognition or machine learning
- G06V10/77—Processing image or video features in feature spaces; using data integration or data reduction, e.g. principal component analysis [PCA] or independent component analysis [ICA] or self-organising maps [SOM]; Blind source separation
- G06V10/7715—Feature extraction, e.g. by transforming the feature space, e.g. multi-dimensional scaling [MDS]; Mappings, e.g. subspace methods
- G—PHYSICS
- G06—COMPUTING OR CALCULATING; COUNTING
- G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
- G06V20/00—Scenes; Scene-specific elements
- G06V20/60—Type of objects
- G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
- G06V20/698—Matching; Classification
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1402—Data analysis by thresholding or gating operations performed on the acquired signals or stored data
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
- G01N2015/1447—Spatial selection
- G01N2015/145—Spatial selection by pattern of light, e.g. fringe pattern

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Engineering & Computer Science (AREA)
Theoretical Computer Science (AREA)
Physics & Mathematics (AREA)
General Physics & Mathematics (AREA)
General Health & Medical Sciences (AREA)
Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Computer Vision & Pattern Recognition (AREA)
Data Mining & Analysis (AREA)
Evolutionary Computation (AREA)
Artificial Intelligence (AREA)
Multimedia (AREA)
Dispersion Chemistry (AREA)
Pathology (AREA)
Immunology (AREA)
Biochemistry (AREA)
Analytical Chemistry (AREA)
Software Systems (AREA)
Medical Informatics (AREA)
Databases & Information Systems (AREA)
Computing Systems (AREA)
Bioinformatics & Computational Biology (AREA)
Evolutionary Biology (AREA)
General Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Biomedical Technology (AREA)
Molecular Biology (AREA)
Signal Processing (AREA)
Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Investigating Or Analysing Biological Materials (AREA)

EP21747161.4A 2020-01-31 2021-01-20 Methods and systems for classifying fluorescent flow cytometer data Active EP4097449B1 (en)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US202062968516P	2020-01-31	2020-01-31
US202063053108P	2020-07-17	2020-07-17
PCT/US2021/014167 WO2021154561A1 (en)	2020-01-31	2021-01-20	Methods and systems for classifying fluorescent flow cytometer data

Publications (3)

Publication Number	Publication Date
EP4097449A1 EP4097449A1 (en)	2022-12-07
EP4097449A4 true EP4097449A4 (en)	2023-07-05
EP4097449B1 EP4097449B1 (en)	2025-08-27

Family

ID=77061839

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP21747161.4A Active EP4097449B1 (en)	2020-01-31	2021-01-20	Methods and systems for classifying fluorescent flow cytometer data

Country Status (4)

Country	Link
US (3)	US11506593B2 (en)
EP (1)	EP4097449B1 (en)
JP (1)	JP7667164B2 (en)
WO (1)	WO2021154561A1 (en)

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WO2021154561A1 (en) *	2020-01-31	2021-08-05	Becton, Dickinson And Company	Methods and systems for classifying fluorescent flow cytometer data
WO2021225792A1 (en) *	2020-05-06	2021-11-11	Becton, Dickinson And Company	Methods and systems for characterizing spillover spreading in flow cytometer data
WO2022076088A1 (en)	2020-10-07	2022-04-14	Becton, Dickinson And Company	Methods for flow cytometry panel design based on modeling and minimizing spillover spreading, and systems for practicing the same
US20230168178A1 (en) *	2021-11-30	2023-06-01	Cytek Biosciences, Inc.	Methods, apparatus, and systems for an optical fiber forward scatter channel in flow cytometers
JP2025508346A (en)	2022-01-31	2025-03-26	ベクトン・ディキンソン・アンド・カンパニー	Method and system for identifying a fluorochrome panel - Patents.com
CN118901102A (en) *	2022-02-14	2024-11-05	贝克顿·迪金森公司	Method and system for group-by-group cytometry data analysis
JP2025523754A (en) *	2022-06-01	2025-07-25	ベクトン・ディキンソン・アンド・カンパニー	Method and system for assessing suitability of a fluorochrome panel for use in generating flow cytometer data - Patents.com
CN115064215B (en) *	2022-08-18	2023-10-24	北京大学人民医院	A method for strain traceability and attribute identification through similarity
EP4634639A1 (en) *	2022-12-12	2025-10-22	Becton, Dickinson and Company	Real-time adaptive methods for spectrally resolving fluorophores of a sample and systems for same
CN117272119B (en) *	2023-11-21	2024-03-22	国网山东省电力公司营销服务中心(计量中心)	User portrait classification model training method, user portrait classification method and system
FR3166970A1 (en)	2024-10-02	2026-04-03	Biomerieux	Method for enumerating microorganisms based on cytometry and associated microbiological control methods.

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US6897954B2 (en)	2002-12-20	2005-05-24	Becton, Dickinson And Company	Instrument setup system for a fluorescence analyzer
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JP4389991B2 (en)	2007-10-26	2009-12-24	ソニー株式会社	Method and apparatus for optical measurement of fine particles
US8415161B2 (en) *	2008-11-13	2013-04-09	Becton, Dickinson And Company	Instrument setup system for a fluorescence analyzer
US20100256943A1 (en)	2009-04-06	2010-10-07	University Of Pittsburgh - Of The Commonwealth System Of Higher Education	Configuration of initial control parameters in photodetectors for multi-color flow cytometry
EP2259045A1 (en)	2009-06-05	2010-12-08	Koninklijke Philips Electronics N.V.	Multi-frequency impedance method and apparatus for discriminating and counting particles expressing a specific marker
US8779387B2 (en) *	2010-02-23	2014-07-15	Accuri Cytometers, Inc.	Method and system for detecting fluorochromes in a flow cytometer
JP5601098B2 (en)	2010-09-03	2014-10-08	ソニー株式会社	Fluorescence intensity correction method and fluorescence intensity calculation apparatus
AU2014228537A1 (en) *	2013-03-15	2015-08-20	Beckman Coulter, Inc.	Systems and methods for panel design in flow cytometry
CA3027341A1 (en)	2016-07-10	2018-01-18	Memed Diagnostics Ltd.	Protein signatures for distinguishing between bacterial and viral infections
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CN112106141A (en)	2018-03-16	2020-12-18	弘泰生物科技股份有限公司	Efficient clustering of immune entities
EP3797282A4 (en) *	2018-05-21	2022-03-16	Cytek Biosciences Inc.	RAPID REWARDING OF FLOW CYTOMETRY DATA FOR OVERFLOW READJUSTMENTS
WO2021154561A1 (en) *	2020-01-31	2021-08-05	Becton, Dickinson And Company	Methods and systems for classifying fluorescent flow cytometer data
WO2021225792A1 (en) *	2020-05-06	2021-11-11	Becton, Dickinson And Company	Methods and systems for characterizing spillover spreading in flow cytometer data

2021
- 2021-01-20 WO PCT/US2021/014167 patent/WO2021154561A1/en not_active Ceased
- 2021-01-20 EP EP21747161.4A patent/EP4097449B1/en active Active
- 2021-01-20 JP JP2022546568A patent/JP7667164B2/en active Active
- 2021-01-20 US US17/153,500 patent/US11506593B2/en active Active
2022
- 2022-10-18 US US17/968,131 patent/US11879829B2/en active Active
2023
- 2023-12-08 US US18/533,763 patent/US12298223B2/en active Active

Non-Patent Citations (3)

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Title
HOLDEN T MAECKER ET AL: "Selecting fluorochrome conjugates for maximum sensitivity", CYTOMETRY A, WILEY-LISS, HOBOKEN, USA, no. 2, 9 November 2004 (2004-11-09), pages 169 - 173, XP072331101, ISSN: 1552-4922, DOI: 10.1002/CYTO.A.20092 *
RICHARD NGUYEN ET AL: "Quantifying spillover spreading for comparing instrument performance and aiding in multicolor panel design", CYTOMETRY A, WILEY-LISS, HOBOKEN, USA, no. 3, 6 February 2013 (2013-02-06), pages 306 - 315, XP072329544, ISSN: 1552-4922, DOI: 10.1002/CYTO.A.22251 *
See also references of WO2021154561A1 *

Also Published As

Publication number	Publication date
EP4097449B1 (en)	2025-08-27
WO2021154561A1 (en)	2021-08-05
JP7667164B2 (en)	2025-04-22
US12298223B2 (en)	2025-05-13
EP4097449A1 (en)	2022-12-07
US20210239592A1 (en)	2021-08-05
US20230045219A1 (en)	2023-02-09
US11506593B2 (en)	2022-11-22
JP2023511760A (en)	2023-03-22
US20240175800A1 (en)	2024-05-30
CN115335680A (en)	2022-11-11
US11879829B2 (en)	2024-01-23

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