EP4129332A4 - Verfahren zur anzeige eines bispezifischen antikörpers auf der oberfläche einer säugerzelle und vektor - Google Patents

Verfahren zur anzeige eines bispezifischen antikörpers auf der oberfläche einer säugerzelle und vektor Download PDF

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Publication number
EP4129332A4
EP4129332A4 EP21774814.4A EP21774814A EP4129332A4 EP 4129332 A4 EP4129332 A4 EP 4129332A4 EP 21774814 A EP21774814 A EP 21774814A EP 4129332 A4 EP4129332 A4 EP 4129332A4
Authority
EP
European Patent Office
Prior art keywords
vector
displaying
mammalian cell
bispecific antibody
bispecific
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP21774814.4A
Other languages
English (en)
French (fr)
Other versions
EP4129332A1 (de
Inventor
Chen Zhou
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ddbio Co Ltd Shang Hai
Original Assignee
Ddbio Co Ltd Shang Hai
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ddbio Co Ltd Shang Hai filed Critical Ddbio Co Ltd Shang Hai
Publication of EP4129332A1 publication Critical patent/EP4129332A1/de
Publication of EP4129332A4 publication Critical patent/EP4129332A4/de
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2818—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against CD28 or CD152
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2827—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against B7 molecules, e.g. CD80, CD86
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y605/00—Ligases forming phosphoric ester bonds (6.5)
    • C12Y605/01—Ligases forming phosphoric ester bonds (6.5) forming phosphoric ester bonds (6.5.1)
    • C12Y605/01001—DNA ligase (ATP) (6.5.1.1)
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
EP21774814.4A 2020-03-27 2021-03-26 Verfahren zur anzeige eines bispezifischen antikörpers auf der oberfläche einer säugerzelle und vektor Pending EP4129332A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202010228279 2020-03-27
PCT/CN2021/083248 WO2021190631A1 (zh) 2020-03-27 2021-03-26 在哺乳动物细胞表面展示双特异性抗体的方法及载体

Publications (2)

Publication Number Publication Date
EP4129332A1 EP4129332A1 (de) 2023-02-08
EP4129332A4 true EP4129332A4 (de) 2024-04-24

Family

ID=77891328

Family Applications (1)

Application Number Title Priority Date Filing Date
EP21774814.4A Pending EP4129332A4 (de) 2020-03-27 2021-03-26 Verfahren zur anzeige eines bispezifischen antikörpers auf der oberfläche einer säugerzelle und vektor

Country Status (8)

Country Link
US (1) US20230331871A1 (de)
EP (1) EP4129332A4 (de)
JP (1) JP2023518898A (de)
KR (1) KR20220160130A (de)
CN (1) CN115315271A (de)
BR (1) BR112022019393A2 (de)
MX (1) MX2022012005A (de)
WO (1) WO2021190631A1 (de)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120101000A1 (en) * 2008-11-21 2012-04-26 Chen Zhou High complexity mammalian display library and methods of screening
EP3608341A1 (de) * 2017-04-01 2020-02-12 Beijing Hanmi Pharmaceutical Co., Ltd. Heterodimerer bispezifischer anti-pd-1/anti-her2-antikörper mit natürlicher antikörperstruktur und herstellung davon

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1303776B1 (it) * 1998-11-19 2001-02-23 S I S S A Scuola Internaz Supe Processo per la preparazione di genoteche, di polipeptidi utilizzandodette genoteche e i polipepti ottenuti.
CN101210241A (zh) * 2006-12-27 2008-07-02 陕西超英生物科技有限公司 一种噬菌体基因工程抗体库基因组装的方法
WO2013092720A1 (en) * 2011-12-22 2013-06-27 F. Hoffmann-La Roche Ag Full length antibody display system for eukaryotic cells and its use
ES2528892T3 (es) * 2012-07-30 2015-02-13 Nbe-Therapeutics Llc Identificación mediada por trasposición de proteínas de unión o funcionales específicas
CN102978713B (zh) * 2012-11-23 2015-02-25 浙江大学 一种白血病单链抗体库及其构建方法和应用
EP3371217B1 (de) * 2015-11-08 2025-06-11 F. Hoffmann-La Roche AG Screening verfahren für multispezifische antikörper
US11492415B2 (en) * 2017-04-07 2022-11-08 Kookmin University Industry Academy Antibody Fc variants for increased blood half-life
WO2019222428A1 (en) * 2018-05-16 2019-11-21 Arbele Limited Composition of bispecific antibodies and method of use thereof
JP7772379B2 (ja) * 2020-03-27 2025-11-18 ディーディーバイオ．カンパニー、リミテッド 抗原特異的結合ポリペプチド遺伝子ディスプレイベクターの構築方法および用途

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120101000A1 (en) * 2008-11-21 2012-04-26 Chen Zhou High complexity mammalian display library and methods of screening
EP3608341A1 (de) * 2017-04-01 2020-02-12 Beijing Hanmi Pharmaceutical Co., Ltd. Heterodimerer bispezifischer anti-pd-1/anti-her2-antikörper mit natürlicher antikörperstruktur und herstellung davon

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
LI C Z ET AL: "Identification of HBsAg-specific antibodies from a mammalian cell displayed full-length human antibody library of healthy immunized donor", CELLULAR & MOLECULAR IMMUNOLOGY, NATURE PUBLISHING GROUP UK, LONDON, vol. 9, no. 2, 1 March 2012 (2012-03-01), pages 184 - 190, XP002743051, ISSN: 1672-7681, [retrieved on 20111219], DOI: 10.1038/CMI.2011.55 *
See also references of WO2021190631A1 *
YASHAS RAJENDRA ET AL: "Transient and stable CHO expression, purification and characterization of novel hetero-dimeric bispecific IgG antibodies", BIOTECHNOLOGY PROGRESS, AMERICAN CHEMICAL SOCIETY, HOBOKEN, USA, vol. 33, no. 2, 3 February 2017 (2017-02-03), pages 469 - 477, XP072292279, ISSN: 8756-7938, DOI: 10.1002/BTPR.2414 *
ZHOU IVAN ET AL: "Four-way ligation for construction of a mammalian cell-based full-length antibody display library", ACTA BIOCHIMICA BIOPHYSICA SINICA, vol. 43, no. 3, 1 March 2011 (2011-03-01), US, pages 232 - 238, XP093140148, ISSN: 1672-9145, Retrieved from the Internet <URL:http://engine.scichina.com/doi/10.1093/abbs/gmq126> DOI: 10.1093/abbs/gmq126 *

Also Published As

Publication number Publication date
WO2021190631A1 (zh) 2021-09-30
JP2023518898A (ja) 2023-05-08
BR112022019393A2 (pt) 2022-12-06
MX2022012005A (es) 2022-10-21
EP4129332A1 (de) 2023-02-08
KR20220160130A (ko) 2022-12-05
US20230331871A1 (en) 2023-10-19
CN115315271A (zh) 2022-11-08

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