ES2183665B1 - Segmentos de adn utiles para seleccionar tipos celulares diferenciados y sus aplicaciones. - Google Patents

Segmentos de adn utiles para seleccionar tipos celulares diferenciados y sus aplicaciones.

Info

Publication number
ES2183665B1
ES2183665B1 ES9902251A ES9902251A ES2183665B1 ES 2183665 B1 ES2183665 B1 ES 2183665B1 ES 9902251 A ES9902251 A ES 9902251A ES 9902251 A ES9902251 A ES 9902251A ES 2183665 B1 ES2183665 B1 ES 2183665B1
Authority
ES
Spain
Prior art keywords
applications
select
dna segments
dna segment
useful dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES9902251A
Other languages
English (en)
Other versions
ES2183665A1 (es
Inventor
Alfonso Valera Abril
Josefa Maria Mayol Belda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad Miguel Hernandez de Elche UMH
Original Assignee
Universidad Miguel Hernandez de Elche UMH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad Miguel Hernandez de Elche UMH filed Critical Universidad Miguel Hernandez de Elche UMH
Priority to ES9902251A priority Critical patent/ES2183665B1/es
Priority to ES200102125A priority patent/ES2208024B1/es
Priority to PCT/ES2000/000391 priority patent/WO2001027266A1/es
Priority to AU77913/00A priority patent/AU7791300A/en
Publication of ES2183665A1 publication Critical patent/ES2183665A1/es
Application granted granted Critical
Publication of ES2183665B1 publication Critical patent/ES2183665B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Segmentos de ADN útiles para seleccionar tipos celulares diferenciados y sus aplicaciones. El segmento de ADN comprende (i) las regiones que contribuyen a formar y estabilizar el complejo de factores de transcripción de un gen específico de un tipo celular que es expresado específicamente en un tipo celular diferenciado, y (ii) un gen de selección funcionalmente conectado, de manera que se exprese cuando el segmento de ADN sea reconocido y transcrito por el complejo de factores de transcripción del gen específico. El segmento de ADN puede utilizarse en la obtención y selección in vitro de tipos celulares diferenciados a partir de unas células precursoras. Con los tipos celulares obtenidos se pueden generar bancos celulares y tisulares con fines terapéuticos o de investigación.
ES9902251A 1999-10-14 1999-10-14 Segmentos de adn utiles para seleccionar tipos celulares diferenciados y sus aplicaciones. Expired - Fee Related ES2183665B1 (es)

Priority Applications (4)

Application Number Priority Date Filing Date Title
ES9902251A ES2183665B1 (es) 1999-10-14 1999-10-14 Segmentos de adn utiles para seleccionar tipos celulares diferenciados y sus aplicaciones.
ES200102125A ES2208024B1 (es) 1999-10-14 1999-10-14 Un metodo para obtener in vitro celulas madre de individuos mamiferos adultos.
PCT/ES2000/000391 WO2001027266A1 (es) 1999-10-14 2000-10-13 Segmentos de adn utiles para seleccionar tipos celulares diferenciados y sus aplicaciones
AU77913/00A AU7791300A (en) 1999-10-14 2000-10-13 Dna segments that are useful for selecting differentiated cell types and the applications thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9902251A ES2183665B1 (es) 1999-10-14 1999-10-14 Segmentos de adn utiles para seleccionar tipos celulares diferenciados y sus aplicaciones.

Publications (2)

Publication Number Publication Date
ES2183665A1 ES2183665A1 (es) 2003-03-16
ES2183665B1 true ES2183665B1 (es) 2004-08-01

Family

ID=8310234

Family Applications (2)

Application Number Title Priority Date Filing Date
ES9902251A Expired - Fee Related ES2183665B1 (es) 1999-10-14 1999-10-14 Segmentos de adn utiles para seleccionar tipos celulares diferenciados y sus aplicaciones.
ES200102125A Expired - Fee Related ES2208024B1 (es) 1999-10-14 1999-10-14 Un metodo para obtener in vitro celulas madre de individuos mamiferos adultos.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES200102125A Expired - Fee Related ES2208024B1 (es) 1999-10-14 1999-10-14 Un metodo para obtener in vitro celulas madre de individuos mamiferos adultos.

Country Status (3)

Country Link
AU (1) AU7791300A (es)
ES (2) ES2183665B1 (es)
WO (1) WO2001027266A1 (es)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9308271D0 (en) * 1993-04-21 1993-06-02 Univ Edinburgh Method of isolating and/or enriching and/or selectively propagating pluripotential animal cells and animals for use in said method
GB9401380D0 (en) * 1994-01-25 1994-03-23 Pharmaceutical Proteins Ltd Embryonic cell isolation
US5753506A (en) * 1996-05-23 1998-05-19 Cns Stem Cell Technology, Inc. Isolation propagation and directed differentiation of stem cells from embryonic and adult central nervous system of mammals
AU6144498A (en) * 1997-02-06 1998-08-26 Osiris Therapeutics, Inc. P21cip1 or p27kip1 effects on the regulation of differentiation of human mesenchymal stem cells
WO1998044142A1 (en) * 1997-04-01 1998-10-08 The General Hospital Corporation Molecular marker for muscle stem cells
AU729377B2 (en) * 1997-10-23 2001-02-01 Asterias Biotherapeutics, Inc. Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HERRERA, P.L. et al. "Two transgenic approaches to define the cell lineages in endocrine pancreas development", MOLECULAR AND CELULAR ENDOCRINOLOGY, 1998, Vol. 140, páginas 45-50. Todo el documento. *
JUN-HSIANG, L. et al. "A tissue-specific promoter that can drive a foreign gene to express in the suprabasal urothelial cells of transgenic mice", PROC. NATL. ACAD. SCI. USA, 1995, Vol. 92, páginas 679-683. Todo el documento. *

Also Published As

Publication number Publication date
ES2208024A1 (es) 2004-06-01
ES2208024B1 (es) 2005-10-01
ES2183665A1 (es) 2003-03-16
WO2001027266A1 (es) 2001-04-19
AU7791300A (en) 2001-04-23

Similar Documents

Publication Publication Date Title
BR0210599A (pt) Uso de monóxido de carbono, método para manutenção de uma célula animal in vitro, artigo de manufatura, bem como meio celular estéril
AR111785A2 (es) Método in vitro de producción de eritrocitos
NO972171L (no) Regulering av neural stamcelleproliferasjon
BR0012099A (pt) Transferência citoplásmica para de-diferenciar células recipientes
BR0015374A (pt) Diferenciação hematopoiética de troncocélulas embriÈnicas humanas
NZ569543A (en) Means and methods for influencing the stability of antibody producing cells
DK1242060T3 (da) Behandling af metastatisk sygdom
EA200870046A1 (ru) Ядерный фактор перепрограммирования
EA201101141A1 (ru) Получение закрытой линейной днк
WO2005007073A3 (en) Ex vivo progenitor and stem cell expansion for use in the treatment of disease of endodermally-derived organs
JP2005532079A5 (es)
DE69941713D1 (de) Abstammungs-spezifische zellen und vorläuferzellen
DK1007537T3 (da) Receptor-protein med betegnelsen 2F1
O’Connor Molecular profiles of cell‐to‐cell variation in the regenerative potential of mesenchymal stromal cells
DE60020855D1 (de) Bedingte immortalisierung von zellen
DE602004008453D1 (de) Künstliches immunorgan
NO20064379L (no) Anvendelse av et serumfritt cellekulturmedium for produsjonen av IL-18BP i pattedyrceller
ES2183665B1 (es) Segmentos de adn utiles para seleccionar tipos celulares diferenciados y sus aplicaciones.
ES8307290A1 (es) Procedimiento para la produccion de un microorganismo que utiliza metanol geneticamente modificado.
ES2584383T3 (es) Promotores preferidos del cámbium/xilema y usos de los mismos
DE60035515D1 (de) Hohe zytokinproduktion mit verbesserte zelllebensfähigkeit
WO2025081199A3 (en) B cell production and expansion
ATE340253T1 (de) Zellkonstrukte erhältlich aus mesenchymalen stammzellen und davon ableitbaren zellen und ihre verwendung
CY1108048T1 (el) Συνθεση σπογγοσινης
SE0200078D0 (sv) Methods for producing and preparing cells for cell therapy

Legal Events

Date Code Title Description
EC2A Search report published

Date of ref document: 20030316

Kind code of ref document: A1

FG2A Definitive protection

Ref document number: 2183665B1

Country of ref document: ES

FD2A Announcement of lapse in spain

Effective date: 20200124