ES8604645A1 - Un metodo de obtencion de celulas huesped de streptomycetes con actividad de restriccion reducida. - Google Patents

Un metodo de obtencion de celulas huesped de streptomycetes con actividad de restriccion reducida.

Info

Publication number
ES8604645A1
ES8604645A1 ES538571A ES538571A ES8604645A1 ES 8604645 A1 ES8604645 A1 ES 8604645A1 ES 538571 A ES538571 A ES 538571A ES 538571 A ES538571 A ES 538571A ES 8604645 A1 ES8604645 A1 ES 8604645A1
Authority
ES
Spain
Prior art keywords
streptomycetes
host cells
dna
obtaining
restriction activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES538571A
Other languages
English (en)
Other versions
ES538571A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Corp Belgium
Pfizer Corp SRL
Original Assignee
Pfizer Corp Belgium
Pfizer Corp SRL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer Corp Belgium, Pfizer Corp SRL filed Critical Pfizer Corp Belgium
Publication of ES538571A0 publication Critical patent/ES538571A0/es
Publication of ES8604645A1 publication Critical patent/ES8604645A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

METODO DE OBTENCION DE CELULAS HUESPED DE STREPTOMYCETES CON ACTIVIDAD DE RESTRICCION REDUCIDA. CONSISTENTE EN: A) MUTAGENESIS DE UNA CEPA DE UNA ESPECIE DE STREPTOMYCETES PARA FORMAR CELULAS HUESPED DE CARACTERISTICAS GENETICAS DIFERENTES; B) CRECIMIENTO DE LAS CELULAS HUESPED Y FORMACION DE PROTOPLASTOS DE LAS MISMAS; C) TRANSFORMAR LOS PROTOPLASTOS CON DNA PLASMIDICO PROCEDENTE DE OTRA FUENTE, SELECCIONADA ENTRE OTRA CEPA DE STREPTOMYCETES, UNA BACTERIA O UNA LEVADURA, CONTENIENDO ESTE DNA UN ORIGEN DE REPLICACION Y UN MARCADOR SELECTIVO PARA EL HUESPED STREPTOMYCETES, ASI COMO CUALESQUIERA SECUENCIAS DE DNA NECESARIA PARA LA REPLICACION Y EL CULTIVO SELECTIVO PROCEDENTES DE OTRA FUENTE; D) REGENERAR LAS CELULAS HUESPED A PARTIR DE LOS PROTOPLASTOS TRANSFORMADOS QUE CONTIENEN EL DNA PLASMIDICO; E) SELECCIONAR LAS CELULAS HUESPED QUE HAN TOMADO EL DNA PLASMIDICO Y HAN SIDO INCAPACES DE RESTRINGIRLO, SEGUIN SELECCION POR LA EXPRESION DEL MARCADOR SELECTIVO; F) CONFIRMAR QUE LAS CELULAS HUESPED PRESENTAN ACTIVIDAD DE RESTRICCION REDUCIDA POR REINTRODUCCION DEL DNA EXTRAÑO POR FORMACION DE PROTOPLASTOS Y TRANSFORMACION SEGUN C), D) Y E).-
ES538571A 1983-12-16 1984-12-13 Un metodo de obtencion de celulas huesped de streptomycetes con actividad de restriccion reducida. Expired ES8604645A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB838333544A GB8333544D0 (en) 1983-12-16 1983-12-16 Streptomycete host cells

Publications (2)

Publication Number Publication Date
ES538571A0 ES538571A0 (es) 1986-02-01
ES8604645A1 true ES8604645A1 (es) 1986-02-01

Family

ID=10553379

Family Applications (1)

Application Number Title Priority Date Filing Date
ES538571A Expired ES8604645A1 (es) 1983-12-16 1984-12-13 Un metodo de obtencion de celulas huesped de streptomycetes con actividad de restriccion reducida.

Country Status (6)

Country Link
EP (1) EP0147145A3 (es)
JP (1) JPS60153792A (es)
AU (1) AU553919B2 (es)
ES (1) ES8604645A1 (es)
GB (1) GB8333544D0 (es)
IL (1) IL73833A0 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8333659D0 (en) * 1983-12-16 1984-01-25 Pfizer Ltd Cosmid cloning vectors
US4898828A (en) * 1985-08-07 1990-02-06 Eli Lilly And Company Ultrahigh copy number streptomycetes plasmids
GB8523768D0 (en) * 1985-09-26 1985-10-30 Antibioticos Sa Streptomyces wadayamensis
US5194387A (en) * 1988-05-09 1993-03-16 Merck & Co., Inc. Process for the incorporation of DNA molecules into microorganisms with methyl-specific restriction systems
US9802327B2 (en) 2013-09-10 2017-10-31 Seiko Epson Corporation Robot arm and robot
JP6337432B2 (ja) 2013-09-10 2018-06-06 セイコーエプソン株式会社 関節駆動装置及びロボット

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58107172A (ja) * 1981-12-18 1983-06-25 Ajinomoto Co Inc 変異株

Also Published As

Publication number Publication date
ES538571A0 (es) 1986-02-01
EP0147145A3 (en) 1987-04-22
JPS60153792A (ja) 1985-08-13
EP0147145A2 (en) 1985-07-03
GB8333544D0 (en) 1984-01-25
AU3667484A (en) 1985-06-20
AU553919B2 (en) 1986-07-31
IL73833A0 (en) 1985-03-31

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ES8604645A1 (es) Un metodo de obtencion de celulas huesped de streptomycetes con actividad de restriccion reducida.