ES8801706A1 - Un procedimiento para preparar un vector de expresion replicable - Google Patents

Un procedimiento para preparar un vector de expresion replicable

Info

Publication number
ES8801706A1
ES8801706A1 ES557478A ES557478A ES8801706A1 ES 8801706 A1 ES8801706 A1 ES 8801706A1 ES 557478 A ES557478 A ES 557478A ES 557478 A ES557478 A ES 557478A ES 8801706 A1 ES8801706 A1 ES 8801706A1
Authority
ES
Spain
Prior art keywords
preparation
modified enzyme
lysine
amino acid
place
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES557478A
Other languages
English (en)
Other versions
ES557478A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beecham Group PLC
Original Assignee
Beecham Group PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB858503358A external-priority patent/GB8503358D0/en
Priority claimed from GB868600979A external-priority patent/GB8600979D0/en
Application filed by Beecham Group PLC filed Critical Beecham Group PLC
Publication of ES8801706A1 publication Critical patent/ES8801706A1/es
Publication of ES557478A0 publication Critical patent/ES557478A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

PROCEDIMIENTO PARA LA PREPARACION DE UN VECTOR DE EXPRESION REPLICABLE A PARTIR DE UN PLASMIDO O UN BACTERIOFOGO DERIVADO DE PRSU-B-GLOBINA. CONTIENE UNA ADN (T-PA) MODIFICADO, QUE POSEE UN AMINOACIDO SIN CADENA LATERAL CARGADA, PREFERENTEMENTE ISOLEUCINA, EN LUGAR DE LISINA EN LA POSICION 277. COMPRENDE: A) PREPARACION DE ADN CODIFICANTE DE T-PA MODIFICADO, PREFERENTEMENTE MEDIANTE LA UTILIZACION DE UN OLIGODESOXIRRIBONUCLEOTIDO COMPLEMENTARIO DEL ADNC DEL T-PA QUE SE EMPAREJE MAL EN LA REGION 1018-1020, DE MODO QUE EL TRIPLETE DEL OLIGODESOXIRRIBONUCLEOTIDO EN LA REGION DE MAL EMPAREJAMIENTO, CORRESPONDA A UN CODON QUE CODIFIQUE ISOLEMINA; B) PREPARACION DE UN VECTOR DE EXPRESION REPLICABLE A PARTIR DE UN PLASMIDO O UN BACTERIOFOGO COMO PRSU-B-GLOBINA QUE CONTIENE EL ADNC DE T-PA MODIFICADO; C) TRANSFORMACION DE UNA CELULA HUESPED CON EL VECTOR DE EXPRESION REPLICABLE; D) RECUPERACION DEL T-PA MODIFICADO. DE APLICACION EN EL TRATAMIENTO DE ENFERMEDADES TROMBOTICAS.
ES557478A 1985-02-09 1987-03-31 Un procedimiento para preparar un vector de expresion replicable Expired ES8801706A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB858503358A GB8503358D0 (en) 1985-02-09 1985-02-09 Compounds
GB868600979A GB8600979D0 (en) 1986-01-16 1986-01-16 Compounds

Publications (2)

Publication Number Publication Date
ES8801706A1 true ES8801706A1 (es) 1988-02-16
ES557478A0 ES557478A0 (es) 1988-02-16

Family

ID=26288793

Family Applications (4)

Application Number Title Priority Date Filing Date
ES551770A Expired ES8706724A1 (es) 1985-02-09 1986-02-07 Un procedimiento para preparar activador de plasminogeno de tipo tejido modificado.
ES557477A Expired ES8800354A1 (es) 1985-02-09 1987-03-31 Un procedimiento para preparar un polimero de adn
ES557479A Expired ES8801707A1 (es) 1985-02-09 1987-03-31 Un procedimiento para preparar una celula huesped transformada con un vector de expresion replicable
ES557478A Expired ES8801706A1 (es) 1985-02-09 1987-03-31 Un procedimiento para preparar un vector de expresion replicable

Family Applications Before (3)

Application Number Title Priority Date Filing Date
ES551770A Expired ES8706724A1 (es) 1985-02-09 1986-02-07 Un procedimiento para preparar activador de plasminogeno de tipo tejido modificado.
ES557477A Expired ES8800354A1 (es) 1985-02-09 1987-03-31 Un procedimiento para preparar un polimero de adn
ES557479A Expired ES8801707A1 (es) 1985-02-09 1987-03-31 Un procedimiento para preparar una celula huesped transformada con un vector de expresion replicable

Country Status (6)

Country Link
EP (1) EP0201153A3 (es)
AU (1) AU5330286A (es)
DK (1) DK62586A (es)
ES (4) ES8706724A1 (es)
GR (1) GR860364B (es)
PT (1) PT81999B (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5445296A (en) * 1992-12-29 1995-08-29 Azkoyen Industrial, S.A. Automatic columns dispensing products

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE81983T1 (de) * 1984-08-08 1992-11-15 Survival Technology Absorptionsverbesserer fuer proteine.
US4661469A (en) * 1984-08-08 1987-04-28 Survival Technology, Inc. t-PA composition capable of being absorbed into the blood stream and method of administration
US5756093A (en) * 1985-04-22 1998-05-26 Genentech, Inc. Tissue plasminogen activator variants
DK175380B1 (da) * 1985-04-22 2004-09-20 Genentech Inc Mutant af human vævsplasminogenaktivator, DNA-sekvens som koder herfor, replicerbar udtrykkelsesvektor til at udtrykke denne DNA-sekvens, mikroorganisme transformeret med denne vektor, cellekulturer transformeret med denne....
US5714372A (en) * 1985-04-22 1998-02-03 Genentech, Inc. Tissue plasminogen activator variants
US5736134A (en) * 1985-04-22 1998-04-07 Genentech, In.C Tissue plasminogen activator variants
US4929444A (en) * 1985-05-28 1990-05-29 Burroughs Wellcome Co. Low pH pharmaceutical formulation containing t-PA
AU605124B2 (en) * 1985-12-23 1991-01-10 Behringwerke Aktiengesellschaft Novel peptide plasminogen activators
DK43387A (da) * 1986-01-29 1987-07-30 Beecham Group Plc Fibrinolytisk enzym
PT84589B (en) * 1986-04-02 1989-05-09 Beecham Group Plc Process for preparing a fibrinolytic enzyme
PT84588B (en) * 1986-04-02 1989-05-09 Beecham Group Plc Process for preparing a fibrinolytic enzyme
GB8619098D0 (en) * 1986-08-05 1986-09-17 Wellcome Found Combination
DK323587D0 (da) * 1987-06-25 1987-06-25 Novo Industri As Protein
WO1988005081A2 (en) * 1986-12-30 1988-07-14 Cetus Corporation Novel plasminogen activator
US5302390A (en) * 1987-07-01 1994-04-12 Beecham Group Plc Hybrid proteins of human plasminogen and human t-PA, pharmaceutical compositions and methods of treatment
IL87276A (en) * 1987-08-03 1995-07-31 Fujisawa Pharmaceutical Co Analog of tissue plasminogen activator comprising only the kringle 2 and protease domain dna encoding the same processes for the preparation thereof and pharmaceutical compositions containing the same
US5108901A (en) * 1988-09-02 1992-04-28 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties
US5714145A (en) * 1988-09-02 1998-02-03 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties
US5258180A (en) * 1988-09-02 1993-11-02 Genetech, Inc. Tissue plasminogen activator having fibrin specific properties and deletion of amino acids 466-970, compositions and methods of treatment
US5262170A (en) * 1988-09-02 1993-11-16 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties and substituted at amino acid positions 296-299, DNA molecules encoding them, vectors, and host cells
US5580560A (en) * 1989-11-13 1996-12-03 Novo Nordisk A/S Modified factor VII/VIIa
US5190756A (en) * 1989-12-01 1993-03-02 Genentech, Inc. Methods and materials for expression of human plasminogen variant
US5087572A (en) * 1989-12-01 1992-02-11 Genentech, Inc. Dna encoding human plasminogen modified at the cleavage site
DK0643772T3 (da) * 1992-06-03 1998-02-02 Genentech Inc Glycosyleringsvarianter af vævsplasminogenaktivator med forbedrede terapeutiske egenskaber
WO1997033984A1 (en) * 1996-03-12 1997-09-18 Novo Nordisk A/S Novel achromobacter lyticus protease variants
DE69738521T2 (de) 1996-04-05 2009-05-07 Novartis Vaccines and Diagnostics, Inc., Emeryville Alphaviren-vektors mit einer reduzierten inhibierung der synthese von zellmakromolekülen
US6706504B1 (en) 1996-11-12 2004-03-16 The Scripps Research Institute Tissue type plasminogen activator (t-PA) variants: compositions and methods of use

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL68561A (en) * 1982-05-05 1991-01-31 Genentech Inc Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof
US4753879A (en) * 1984-08-27 1988-06-28 Biogen N.V. Modified tissue plasminogen activators
DK175380B1 (da) * 1985-04-22 2004-09-20 Genentech Inc Mutant af human vævsplasminogenaktivator, DNA-sekvens som koder herfor, replicerbar udtrykkelsesvektor til at udtrykke denne DNA-sekvens, mikroorganisme transformeret med denne vektor, cellekulturer transformeret med denne....

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5445296A (en) * 1992-12-29 1995-08-29 Azkoyen Industrial, S.A. Automatic columns dispensing products

Also Published As

Publication number Publication date
DK62586A (da) 1986-08-10
ES557477A0 (es) 1987-10-16
ES551770A0 (es) 1987-07-01
ES8801707A1 (es) 1988-02-16
GR860364B (en) 1986-06-10
AU5330286A (en) 1986-08-14
EP0201153A3 (en) 1987-10-07
EP0201153A2 (en) 1986-11-12
PT81999A (en) 1986-03-01
ES8800354A1 (es) 1987-10-16
ES557479A0 (es) 1988-02-16
ES8706724A1 (es) 1987-07-01
PT81999B (en) 1987-12-22
DK62586D0 (da) 1986-02-07
ES557478A0 (es) 1988-02-16

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