JP2017519774A5 - - Google Patents

Download PDF

Info

Publication number
JP2017519774A5
JP2017519774A5 JP2016574392A JP2016574392A JP2017519774A5 JP 2017519774 A5 JP2017519774 A5 JP 2017519774A5 JP 2016574392 A JP2016574392 A JP 2016574392A JP 2016574392 A JP2016574392 A JP 2016574392A JP 2017519774 A5 JP2017519774 A5 JP 2017519774A5
Authority
JP
Japan
Prior art keywords
nucleic acid
optionally
target nucleic
tag
proteases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2016574392A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017519774A (ja
JP6626848B2 (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2015/037653 external-priority patent/WO2015200609A1/en
Publication of JP2017519774A publication Critical patent/JP2017519774A/ja
Publication of JP2017519774A5 publication Critical patent/JP2017519774A5/ja
Application granted granted Critical
Publication of JP6626848B2 publication Critical patent/JP6626848B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2016574392A 2014-06-26 2015-06-25 単一の管を付加したプロトコルを用いるタグ化核酸ライブラリの調製 Active JP6626848B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201462017786P 2014-06-26 2014-06-26
US62/017,786 2014-06-26
US201462027198P 2014-07-21 2014-07-21
US62/027,198 2014-07-21
PCT/US2015/037653 WO2015200609A1 (en) 2014-06-26 2015-06-25 Library preparation of tagged nucleic acid using single tube add-on protocol

Publications (3)

Publication Number Publication Date
JP2017519774A JP2017519774A (ja) 2017-07-20
JP2017519774A5 true JP2017519774A5 (2) 2018-06-14
JP6626848B2 JP6626848B2 (ja) 2019-12-25

Family

ID=53761498

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2016574392A Active JP6626848B2 (ja) 2014-06-26 2015-06-25 単一の管を付加したプロトコルを用いるタグ化核酸ライブラリの調製

Country Status (9)

Country Link
US (3) US10017759B2 (2)
EP (2) EP3161137B1 (2)
JP (1) JP6626848B2 (2)
CN (2) CN106795651B (2)
AU (1) AU2015279862B2 (2)
CA (1) CA2953367A1 (2)
DK (1) DK3161137T3 (2)
ES (2) ES3031891T3 (2)
WO (1) WO2015200609A1 (2)

Families Citing this family (123)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5843614B2 (ja) 2009-01-30 2016-01-13 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
ES2625288T3 (es) 2011-04-15 2017-07-19 The Johns Hopkins University Sistema de secuenciación segura
AU2012288629B2 (en) 2011-07-25 2017-02-02 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
DK2814959T3 (en) 2012-02-17 2018-04-23 Hutchinson Fred Cancer Res COMPOSITIONS AND PROCEDURES FOR EXACTLY IDENTIFYING MUTATIONS
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
CA3216609C (en) 2012-08-14 2024-05-14 10X Genomics, Inc. Microcapsule compositions and methods
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
AU2013338393C1 (en) 2012-10-29 2024-07-25 The Johns Hopkins University Papanicolaou test for ovarian and endometrial cancers
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
AU2014214682B2 (en) 2013-02-08 2018-07-26 10X Genomics, Inc. Polynucleotide barcode generation
KR102168813B1 (ko) 2013-03-08 2020-10-22 옥스포드 나노포어 테크놀로지즈 리미티드 효소 정지 방법
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
EP4321628B1 (en) 2013-05-23 2025-09-03 The Board of Trustees of the Leland Stanford Junior University Transposition into native chromatin for personal epigenomics
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
WO2015157567A1 (en) 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
US20150353989A1 (en) * 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
CN113249435B (zh) 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
MX2016016904A (es) 2014-06-26 2017-03-27 10X Genomics Inc Analisis de secuencias de acidos nucleicos.
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
MX2017005267A (es) 2014-10-29 2017-07-26 10X Genomics Inc Metodos y composiciones para la secuenciacion de acidos nucleicos seleccionados como diana.
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
DK3253911T3 (da) 2015-02-04 2021-04-06 Univ British Columbia Fremgangsmåder og anordninger til analyse af partikler
EP4286516A3 (en) 2015-02-24 2024-03-06 10X Genomics, Inc. Partition processing methods and systems
BR112017018054A2 (pt) 2015-02-24 2018-07-24 10X Genomics Inc métodos para a cobertura de sequências de ácidos nucleicos direcionadas
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
AU2016343937B2 (en) * 2015-10-30 2023-01-19 Exact Sciences Corporation Multiplex amplification detection assay and isolation and detection of DNA from plasma
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
JP6735348B2 (ja) 2016-02-11 2020-08-05 １０エックス ジェノミクス， インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
JP6882453B2 (ja) * 2016-08-31 2021-06-02 プレジデント アンド フェローズ オブ ハーバード カレッジ 全ゲノムデジタル増幅方法
EP3519612B1 (en) * 2016-10-01 2022-04-06 Berkeley Lights, Inc. Dna barcode compositions and methods of in situ identification in a microfluidic device
IL266197B2 (en) 2016-10-24 2024-03-01 Geneinfosec Inc Hiding information contained within nucleic acids
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018119399A1 (en) 2016-12-23 2018-06-28 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
AU2017386533B2 (en) * 2016-12-29 2024-05-09 Illumina, Inc. Analysis system for orthogonal access to and tagging of biomolecules in cellular compartments
CN106801044B (zh) * 2017-01-09 2021-03-23 北京全式金生物技术有限公司 一种环状转座子复合物及其应用
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
CN110214186B (zh) 2017-01-30 2023-11-24 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和系统
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
US11274344B2 (en) 2017-03-30 2022-03-15 Grail, Inc. Enhanced ligation in sequencing library preparation
US11584958B2 (en) 2017-03-31 2023-02-21 Grail, Llc Library preparation and use thereof for sequencing based error correction and/or variant identification
WO2018183897A1 (en) 2017-03-31 2018-10-04 Grail, Inc. Higher target capture efficiency using probe extension
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
IL319255A (en) 2017-08-07 2025-04-01 Univ Johns Hopkins Methods and materials for cancer assessment and treatment
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
CN114525273B (zh) 2017-10-27 2025-01-28 10X基因组学有限公司 用于样品制备和分析的方法和系统
EP3954782A1 (en) 2017-11-15 2022-02-16 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
CN118818037A (zh) 2017-12-12 2024-10-22 10X基因组学有限公司 用于单细胞处理的系统和方法
CN118547046A (zh) 2017-12-22 2024-08-27 10X基因组学有限公司 用于处理来自一个或多个细胞的核酸分子的系统和方法
WO2019129133A1 (zh) * 2017-12-28 2019-07-04 深圳华大智造科技有限公司 一种获得单细胞mRNA序列的方法
CN112004920B (zh) 2018-02-05 2024-08-02 斯坦福大学托管董事会 用于单细胞和集合细胞的多重测量的系统和方法
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
CN112262218B (zh) 2018-04-06 2024-11-08 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
CN120624607A (zh) 2018-05-08 2025-09-12 深圳华大智造科技股份有限公司 用于准确且经济高效的测序、单体型分型和组装的基于单管珠粒的dna共条形码化
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
CN111247248A (zh) * 2018-06-04 2020-06-05 伊鲁米纳公司 高通量单细胞转录组文库及制备和使用方法
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US20210222163A1 (en) * 2018-07-11 2021-07-22 X Gen Us Co. Transposome enabled dna/rna-sequencing (ted rna-seq)
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
EP3830289B1 (en) 2018-08-03 2026-02-11 10X Genomics, Inc. Methods to minimize barcode exchange
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
CN109295048A (zh) * 2018-10-09 2019-02-01 中国农业科学院深圳农业基因组研究所 一种全基因组分子标记检测的方法
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
WO2020157684A1 (en) * 2019-01-29 2020-08-06 Mgi Tech Co., Ltd. High coverage stlfr
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
SG11202108788TA (en) 2019-02-12 2021-09-29 10X Genomics Inc Methods for processing nucleic acid molecules
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
CN113767178A (zh) 2019-03-11 2021-12-07 10X基因组学有限公司 用于处理光学标签化珠粒的系统和方法
CN113811619A (zh) 2019-03-27 2021-12-17 10X基因组学有限公司 用于处理来自细胞的rna的系统和方法
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
GB201914266D0 (en) * 2019-10-03 2019-11-20 Univ Oxford Innovation Ltd Method of amplifying nucleic acids
EP3927824A2 (en) * 2019-12-19 2021-12-29 Illumina, Inc. High-throughput single-cell libraries and methods of making and of using
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
JP2023513606A (ja) 2020-02-14 2023-03-31 ザ・ジョンズ・ホプキンス・ユニバーシティー 核酸を評価するための方法および材料
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
CN111575348B (zh) * 2020-05-19 2024-01-09 广州微远医疗器械有限公司 宏基因组文库及建库方法和应用
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
JP2023551072A (ja) 2020-11-25 2023-12-06 アリダ・バイオサイエンシーズ・インコーポレイテッド Ｒｎａおよびｄｎａ修飾の多重プロファイリング
EP4279609B1 (en) * 2020-12-31 2026-04-08 Beijing Institute of Genomics, Chinese Academy of Sciences (China National Center for Bioinformation) Method and kit for labeling nucleic acid molecules
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
CN120158497A (zh) 2021-02-23 2025-06-17 10X基因组学有限公司 基于探针的核酸和蛋白质分析
CN118318038A (zh) * 2021-11-10 2024-07-09 东洋纺株式会社 改良的文库制备方法
WO2023097295A1 (en) 2021-11-24 2023-06-01 Alida Biosciences, Inc. Rna and dna analysis using engineered surfaces
CN116408160B (zh) * 2021-12-31 2025-11-25 彩科(苏州)生物科技有限公司 具有稳定的隔离环境的微流体装置
WO2025136701A1 (en) * 2023-12-22 2025-06-26 Illumina, Inc. Passive encapsulated workflows

Family Cites Families (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
US5834202A (en) 1992-08-04 1998-11-10 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5614389A (en) 1992-08-04 1997-03-25 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US6261808B1 (en) 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US5386024A (en) 1993-02-10 1995-01-31 Gen-Probe Incorporated Method to prepare nucleic acids from a biological sample using low pH and acid protease
US5422252A (en) 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
CA2122203C (en) 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
WO1995003430A1 (en) 1993-07-23 1995-02-02 Gen-Probe Incorporated Methods for enhancing nucleic acid amplification
FR2708288B1 (fr) 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
US5523204A (en) 1993-12-10 1996-06-04 Becton Dickinson And Company Detection of nucleic acids in cells by strand displacement amplification
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
WO1995025180A1 (en) 1994-03-16 1995-09-21 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
FR2726277B1 (fr) 1994-10-28 1996-12-27 Bio Merieux Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin
US5650288A (en) * 1995-07-14 1997-07-22 Macfarlane; Gordon D. Immunophilin-bound immunosuppressant assay
US5916779A (en) 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
US5631147A (en) 1995-09-21 1997-05-20 Becton, Dickinson And Company Detection of nucleic acids in cells by thermophilic strand displacement amplification
JP2000505312A (ja) 1996-03-18 2000-05-09 モレキュラー バイオロジー リソーシーズ，インコーポレイテッド 標的核酸配列増幅
US5773733A (en) 1996-04-12 1998-06-30 National Science Council Alumina-aluminum nitride-nickel composites
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
JP2002503954A (ja) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
ATE374833T1 (de) 1998-11-09 2007-10-15 Eiken Chemical Verfahren zur synthese von nukleinsäuren
US6155972A (en) 1999-02-02 2000-12-05 Acorn Cardiovascular, Inc. Cardiac constraint jacket construction
WO2000056877A1 (en) 1999-03-19 2000-09-28 Takara Shuzo Co., Ltd. Method for amplifying nucleic acid sequence
US6326173B1 (en) 1999-04-12 2001-12-04 Nanogen/Becton Dickinson Partnership Electronically mediated nucleic acid amplification in NASBA
US6309833B1 (en) 1999-04-12 2001-10-30 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures
US6238868B1 (en) 1999-04-12 2001-05-29 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology
US20050181440A1 (en) 1999-04-20 2005-08-18 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
MXPA02002656A (es) 1999-09-13 2003-10-14 Nugen Technologies Inc Metodos y composiciones para la ampliacion lineal isotermica de secuencias de polinucleotidos.
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
WO2002004680A2 (en) 2000-07-07 2002-01-17 Visigen Biotechnologies, Inc. Real-time sequence determination
CN1254548C (zh) 2000-08-23 2006-05-03 宝生物工程株式会社 核酸扩增方法
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US20040038213A1 (en) * 2002-08-06 2004-02-26 Kwon Jai W. Genotyping by in situ PCR amplification of a polynucleotide in a tissue biopsy
EP3002289B1 (en) 2002-08-23 2018-02-28 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
CN1738914A (zh) 2002-11-21 2006-02-22 震源技术公司 使用编码双链启动子一条链的引物的方法
US20050153333A1 (en) 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids
FR2865731B1 (fr) 2004-01-30 2007-09-07 Solvay Procede de fabrication d'un hydrofluoroalcane
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
US20060188892A1 (en) 2005-02-18 2006-08-24 Ambion, Inc. Enzymatic digestion of tissue
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
EP3722409A1 (en) 2006-03-31 2020-10-14 Illumina, Inc. Systems and devices for sequence by synthesis analysis
EP3305915B1 (en) * 2006-08-01 2022-01-26 Applied Biosystems, LLC Detection of analytes and nucleic acids
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US7948015B2 (en) 2006-12-14 2011-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
JP5794572B2 (ja) * 2008-10-24 2015-10-14 エピセンター テクノロジーズ コーポレイションＥｐｉｃｅｎｔｒｅ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ｃｏｒｐｏｒａｔｉｏｎ 核酸を修飾するためのトランスポゾン末端組成物及び方法
US9080211B2 (en) * 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
WO2011026194A1 (en) 2009-09-07 2011-03-10 Reproductive Health Science Pty Ltd Nucleic acid extraction
EP2388312A1 (en) * 2010-05-17 2011-11-23 Curetis AG Universally applicable lysis buffer and processing methods for the lysis of bodily samples
US20130123120A1 (en) * 2010-05-18 2013-05-16 Natera, Inc. Highly Multiplex PCR Methods and Compositions
EP2632593B1 (en) 2010-10-27 2021-09-29 Illumina, Inc. Flow cells for biological or chemical analysis
US10457936B2 (en) * 2011-02-02 2019-10-29 University Of Washington Through Its Center For Commercialization Massively parallel contiguity mapping
HRP20211595T1 (hr) * 2011-05-24 2022-01-21 BioNTech SE Individualizirana cjepiva protiv raka
US10689643B2 (en) 2011-11-22 2020-06-23 Active Motif, Inc. Targeted transposition for use in epigenetic studies
NO2694769T3 (2) * 2012-03-06 2018-03-03
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
AU2014214682B2 (en) * 2013-02-08 2018-07-26 10X Genomics, Inc. Polynucleotide barcode generation
US20160208323A1 (en) 2013-06-21 2016-07-21 The Broad Institute, Inc. Methods for Shearing and Tagging DNA for Chromatin Immunoprecipitation and Sequencing
US20150353989A1 (en) * 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
DK3253911T3 (da) 2015-02-04 2021-04-06 Univ British Columbia Fremgangsmåder og anordninger til analyse af partikler

Similar Documents

Publication Publication Date Title
JP2017519774A5 (2)
JP6925424B2 (ja) 短いｄｎａ断片を連結することによる一分子シーケンスのスループットを増加する方法
JP6483249B2 (ja) 単離されたオリゴヌクレオチドおよび核酸の配列決定におけるその使用
CN105745334B (zh) 通过使用邻近条形编码分析分子复合物的概况的方法
US11401543B2 (en) Methods and compositions for improving removal of ribosomal RNA from biological samples
US20220127597A1 (en) Haplotagging - haplotype phasing and single-tube combinatorial barcoding of nucleic acid molecules using bead-immobilized tn5 transposase
JP2013544498A5 (2)
JP2020505924A (ja) 競合的鎖置換を利用する次世代シーケンシング（ｎｇｓ）ライブラリーの構築
JP2007537751A5 (2)
Niedzicka et al. Molecular Inversion Probes for targeted resequencing in non-model organisms
ES2645423T3 (es) Método de análisis de ARN de conservación del número de copias
CA2892646A1 (en) Methods for targeted genomic analysis
JP7653924B2 (ja) 近接ライゲーションのための方法および組成物
JP7539770B2 (ja) ゲノム再編成検出のための配列決定方法
JP2024502028A (ja) 配列決定ライブラリー調製のための方法および組成物
Jung et al. Isothermal target and signaling probe amplification method, based on a combination of an isothermal chain amplification technique and a fluorescence resonance energy transfer cycling probe technology
CN101532014A (zh) 对目标基因组区域边扩增边连接的公用接头及连接方法
JP2019176859A (ja) クランプオリゴヌクレオチドを利用する核酸増幅方法
JP6074036B2 (ja) 拡大された基質範囲を有する新規のｄｎａポリメラーゼ
CN114364813B (zh) 多重等温扩增核酸序列的方法
CN104531874B (zh) Egfr基因突变检测方法及试剂盒
US20110269194A1 (en) Materials and methods for nucleic acid fractionation by solid phase entrapment and enzyme-mediated detachment
Nikiforova et al. Amplification-based methods
Maan et al. Novel molecular diagnostics and therapeutic tools for livestock diseases
CN117881796A (zh) 使用靶向表观遗传测定、邻近诱导标签化、链侵入、限制或连接来检测分析物