JPH01215277A - Rotary drum bioreactor having separated chambers - Google Patents
Rotary drum bioreactor having separated chambersInfo
- Publication number
- JPH01215277A JPH01215277A JP4005188A JP4005188A JPH01215277A JP H01215277 A JPH01215277 A JP H01215277A JP 4005188 A JP4005188 A JP 4005188A JP 4005188 A JP4005188 A JP 4005188A JP H01215277 A JPH01215277 A JP H01215277A
- Authority
- JP
- Japan
- Prior art keywords
- rotating drum
- immobilized biocatalyst
- bioreactor
- rotary
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000004190 Enzymes Human genes 0.000 claims abstract description 30
- 108090000790 Enzymes Proteins 0.000 claims abstract description 30
- 239000011942 biocatalyst Substances 0.000 claims abstract description 23
- 238000006243 chemical reaction Methods 0.000 claims abstract description 16
- 239000007789 gas Substances 0.000 claims description 9
- 244000005700 microbiome Species 0.000 claims description 9
- 238000000855 fermentation Methods 0.000 claims description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 7
- 238000010564 aerobic fermentation Methods 0.000 claims description 4
- 239000003570 air Substances 0.000 claims description 4
- UBAZGMLMVVQSCD-UHFFFAOYSA-N carbon dioxide;molecular oxygen Chemical compound O=O.O=C=O UBAZGMLMVVQSCD-UHFFFAOYSA-N 0.000 claims description 4
- 239000000919 ceramic Substances 0.000 claims description 3
- 239000000835 fiber Substances 0.000 claims description 3
- 229920005615 natural polymer Polymers 0.000 claims description 3
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- 229920000642 polymer Polymers 0.000 claims description 3
- 229920002994 synthetic fiber Polymers 0.000 claims description 3
- 239000012209 synthetic fiber Substances 0.000 claims description 3
- 229920001059 synthetic polymer Polymers 0.000 claims description 3
- 125000006850 spacer group Chemical group 0.000 claims 1
- 239000000758 substrate Substances 0.000 abstract description 11
- 239000012295 chemical reaction liquid Substances 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 239000003054 catalyst Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 3
- 241000218691 Cupressaceae Species 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000005465 channeling Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 239000000969 carrier Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000000498 cooling water Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- -1 lumps Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/06—Stirrer or mobile mixing elements with horizontal or inclined stirrer shaft or axis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Sustainable Development (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【発明の詳細な説明】
［゛産業上の利用分野」
本発明は微生物あるいは酵素を適当な担体に保持させた
固定化生体触媒を利用して有用物質を生産するバイオリ
アクターに関するものである。DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a bioreactor that produces useful substances using an immobilized biocatalyst in which microorganisms or enzymes are supported on a suitable carrier.
「従来の技術」
従来型のバイオリアクターでは微生物、酵素の固定化方
法として担体結合法、架橋法、包括法、あるいはこれら
を組み合せた複合法等が用いられ、これらの方法により
得られる固定化生体触媒の粒状、塊状、フィルム状、シ
ート状などの固定化形態により充填層型、流動層型、換
型、膜型、仮型等のりアクタ−に使いわけて利用してい
る。又回転円板方式も数機種開発されており高い攪拌効
率、圧力損失が少いこと、円板の着脱を容易にして生体
触媒の反応活性を維持して、生成物収率を高めることか
できる等の点でバイオリアクターとして優れた特質を持
っている。しかしこの回転円板式バイオリアクターでは
円板上に菌体を着床させる念めに使用可能微生物に制限
がある事、発酵を一室で実施するため固定化生体触媒の
活性が一様に低下し、活性を維持し製品濃度を一定に保
つために多数の回転円板を必要とし、その結果、設置面
積が予想外に大きくなる事、同軸上に並設した回転円板
間の間隔が狭隘となり円板間隙に増殖した菌体層が肥厚
しカラム型におけるチャンネリングに似た現象を生じ生
成物転換率が低下する事等の欠点がある。"Conventional technology" In conventional bioreactors, carrier binding methods, crosslinking methods, entrapment methods, or composite methods combining these methods are used to immobilize microorganisms and enzymes. Depending on the immobilization form of the catalyst, such as granules, lumps, films, and sheets, it is used for glue actors such as packed bed type, fluidized bed type, exchange type, membrane type, and temporary type. In addition, several types of rotating disk systems have been developed, which have high stirring efficiency, low pressure loss, and can easily attach and detach the disks to maintain the reaction activity of the biocatalyst and increase product yield. It has excellent characteristics as a bioreactor. However, in this rotating disk type bioreactor, there are restrictions on the number of microorganisms that can be used to ensure that bacterial cells can be implanted on the disk, and because fermentation is carried out in one chamber, the activity of the immobilized biocatalyst decreases uniformly. In order to maintain activity and maintain a constant product concentration, a large number of rotating disks are required, resulting in an unexpectedly large installation area and a narrow spacing between the rotating disks installed on the same axis. There are drawbacks such as a thickening of the bacterial layer grown in the disc space, a phenomenon similar to channeling in a column type, and a decrease in product conversion.
「発明が解決しようとする問題点」
本発明は上記の事情に鑑み使用微生物の適用範囲を拡大
すると共に基質と生体触媒の有効接触率の向上とチャン
ネリング現象の防止をはかり、固定化生体触媒にかかる
負荷を段階的に軽減せしめ触媒の活性を平均的に維持し
、製品の品質安定化をはかることを目的とするものであ
る。"Problems to be Solved by the Invention" In view of the above circumstances, the present invention expands the scope of application of microorganisms used, improves the effective contact rate between the substrate and biocatalyst, and prevents the channeling phenomenon, and provides an immobilized biocatalyst. The purpose of this is to gradually reduce the load on the catalyst, maintain an average activity of the catalyst, and stabilize the quality of the product.
「問題点を解決するための手段」
本発明は上記の目的を達成するため
横−円筒密閉型槽内を数個の反応室に区分し各室に回転
軸を軸受上で連結する着脱任意で、しかも固定化生体触
媒を充填保持でき、反応液が容易に出入りできる孔を有
する回転ドラムを有することを特徴とするバイオリアク
ターを特定発明とするものであって、
天然高分子ゲル、合成高分子ゲル、天然繊維、合成繊維
、多孔質高分子、多孔質セラミックス等の担体に包括あ
るいは架橋、吸着された微生物まなは酵素よりなる固定
化生体触媒を回転ドラム内に充填する上記特定発明記載
のバイオリアクター、空気、酸素、炭酸ガス、窒素等槽
内供給ガスの変換および固定化生体触媒の交換により好
気性発酵、通性嫌気性発酵いずれをも実施可能とする上
記特定発明記載のバイオリアクターによって構成される
。"Means for Solving the Problems" In order to achieve the above-mentioned object, the present invention divides the interior of a horizontal-cylindrical closed tank into several reaction chambers, and connects a rotating shaft to each chamber on a bearing. A specific invention is a bioreactor characterized by having a rotating drum capable of being filled and held with an immobilized biocatalyst and having holes through which a reaction solution can easily enter and exit, the bioreactor being a natural polymer gel, a synthetic polymer, etc. The bio-catalyst according to the above-mentioned specific invention, in which a rotating drum is filled with an immobilized biocatalyst consisting of microorganisms or enzymes that is entrapped, cross-linked, or adsorbed on a carrier such as gel, natural fiber, synthetic fiber, porous polymer, porous ceramic, or the like. The reactor is composed of the bioreactor described in the above-mentioned specified invention, which is capable of carrying out both aerobic fermentation and facultative anaerobic fermentation by converting the gases supplied in the tank such as air, oxygen, carbon dioxide, and nitrogen, and exchanging the immobilized biocatalyst. be done.
「作用」
従って４ｔｔ＆円筒密閉型檜（リアクター槽）１０本体
１から蓋２を外し、各反応室８．８．８内に回転軸１１
によって支持されている有孔回転ドラム１５．１５．１
５内に固定化生体触媒を充填したジャケット１６に恒温
水を循環させ、リアクター槽ｌ内にガス導入口５から使
用ガスを導入し蒸発成分凝縮器に冷却水を通水する。こ
の状態にお−て上記回転ドラム１５．１５．１５を回動
し１基質注入口４から基質を注入り、リアクター槽１の
液性制御回路を作動して運転する。従って基質は反応液
溢流口１４．１４を溢流して反応室８を通過すると同時
に上記回転ドラム１５％１５．１５内の固定化生体触媒
によって目的生成物成分の濃度が除々に上り、リアクタ
ー槽ｌの生成物溢流口１Ｂ、１８から目標濃度の生成物
が溢流する〇又必要に応じて下部試料採取口１９からサ
ンプリングして発酵状態を掌握し１又必要に応じて上記
１１１の底部の、ガス散気口２０よりガスを供給するこ
とができる。"Operation" Therefore, remove the lid 2 from the 4tt & cylindrical closed type cypress (reactor tank) 10 body 1, and insert the rotating shaft 11 into each reaction chamber 8.8.8.
perforated rotating drum supported by 15.15.1
Constant-temperature water is circulated through a jacket 16 filled with an immobilized biocatalyst, a gas to be used is introduced into the reactor tank l from the gas inlet 5, and cooling water is passed through the evaporated component condenser. In this state, the rotating drum 15, 15, 15 is rotated to inject a substrate from the substrate injection port 4, and the liquid control circuit of the reactor tank 1 is activated to operate. Therefore, the substrate overflows the reaction liquid overflow port 14.14 and passes through the reaction chamber 8, and at the same time, the concentration of the target product component gradually increases due to the immobilized biocatalyst in the rotating drum 15% 15.15, and the concentration of the target product component increases gradually. The product of the target concentration overflows from the product overflow ports 1B and 18 of 1. If necessary, the product is sampled from the lower sampling port 19 to grasp the fermentation state. Gas can be supplied from the gas diffuser port 20 .
「実施例」
・本発明の実施例を添付図面によって以下詳述する。第
１図は隔室回転ドラム式バイオリアクターの縦断面図、
第２図はバイオリアクター本体の分解斜視図、第３図は
回転ドラムの分解斜視図を示している０１は上半部の蓋
２と下半部の本体１′を有する横置円筒密閉型槽、蓋２
は槽内液のＰＨを一定に保つための薬液注入口３、基質
注入口４、好気性発酵、通性嫌気性発酵等の発酵形態に
応じて供給する空気、酸素、炭酸ガス、窒素等のガス導
入口５、蓋体内部を三個の反応室８に区分し通気口６．
６を有するａ １１７ ｓ ’ｙを設け、反応制御のた
めのセンサー挿入口９、蒸発成分凝縮器を接続する脱気
口１０、蓋２及び本体１の両端壁中心部および隔壁中心
部に回転軸１１のシール装備を有する軸受１２を設け、
蓋２を本体ｌと締結する蝶型ネジ１３を具備し１上記隔
Ｍｌ！７．７には反応液溢流口１４．１４’を設ける。"Embodiments" - Examples of the present invention will be described in detail below with reference to the accompanying drawings. Figure 1 is a longitudinal cross-sectional view of a compartment rotating drum bioreactor.
Fig. 2 is an exploded perspective view of the bioreactor main body, and Fig. 3 is an exploded perspective view of the rotating drum. , lid 2
The chemical solution inlet 3 and the substrate inlet 4 are used to keep the pH of the solution in the tank constant, and air, oxygen, carbon dioxide, nitrogen, etc. are supplied depending on the type of fermentation such as aerobic fermentation, facultative anaerobic fermentation, etc. A gas inlet 5, the inside of the lid is divided into three reaction chambers 8, and a vent 6.
6, a sensor insertion port 9 for reaction control, a deaeration port 10 for connecting the evaporated component condenser, and a rotating shaft at the center of both end walls of the lid 2 and main body 1 and the center of the partition wall. A bearing 12 having 11 seals is provided,
A butterfly-shaped screw 13 is provided for fastening the lid 2 to the main body l, and the distance Ml is above 1! 7.7 is provided with a reaction liquid overflow port 14.14'.
上記回転軸１１には上記反応室８の内部に網製有孔同転
ドラム１５を設け、該ドラム１５には固定化生体触媒を
充填する扉１５が設けられる０又本体１の外周には槽温
度を一定に保つためのジャケラ）１６、ジャケット用恒
温循環水人出口１７．１７％生成物溢流ロ１８．１８、
試料採取口１９、ＷＩ底部からの供給ガス散気口２０、
蓋２と本体１のシール用埋込保持した各個着脱任意の回
転ドラム１５がシール軸受上に懸架された状態で回転軸
１１方向に並設され、回転軸１１の一端は閉塞軸受１２
．１２に支持され、他端は上記檜ｌ外に設置する回転ド
ラムｌ１ｉ（動装＆２３にギアヘッド２４を介して接続
される。固定化生体触媒は天然高分子ゲル、合成高分子
ゲル、天然繊維、合成繊維、多孔質高分子、多孔質セラ
ミックス等による担体に包括、架橋或は吸着された微生
物又は酵素であり、使用ガスは空気、酸素、炭酸ガス、
窒素ガス等を変換可能であり、かつ上記固定化生体触媒
を交換又は変換することによって上記ｌ！１１内におい
て好気性発酵や通性嫌気性発酵を行うことができるもの
である。The rotary shaft 11 is provided with a perforated mesh drum 15 inside the reaction chamber 8, and the drum 15 is provided with a door 15 for filling the immobilized biocatalyst. Jacquera to keep the temperature constant) 16, constant temperature circulation water outlet for jacket 17.17% product overflow 18.18,
Sample collection port 19, supply gas diffuser port 20 from the bottom of the WI,
Optional rotary drums 15 for sealing the lid 2 and the main body 1 are installed in parallel in the direction of the rotary shaft 11 while being suspended on seal bearings, and one end of the rotary shaft 11 is connected to a closed bearing 12.
．． 12, and the other end is connected via a gear head 24 to a rotating drum l1i (moving equipment &23) installed outside the cypress l.The immobilized biocatalyst is a natural polymer gel, a synthetic polymer gel, a natural fiber, Microorganisms or enzymes are encapsulated, crosslinked, or adsorbed on a carrier such as synthetic fibers, porous polymers, or porous ceramics, and the gases used are air, oxygen, carbon dioxide,
It is possible to convert nitrogen gas, etc., and the above-mentioned l! 11, aerobic fermentation and facultative anaerobic fermentation can be carried out.
尚上記回転軸１１は軸受上で一体に連結した１個の共通
回転軸１１でも又軸受上で回転方向に固定した分離可能
な共通中心線上の３個の回転軸１１でも差支えない。The rotating shaft 11 may be one common rotating shaft 11 integrally connected on a bearing, or three rotating shafts 11 on a common centerline that are separable and fixed in the rotational direction on a bearing.
「発明の効果」
本発明は回転ドラムの採用により使用微生物又は酵素の
適用範囲が広い事、リアクター槽内を数案に区分し基質
溶液を順次次室へ溢流移動させることにより固定化生体
触媒にかかる負荷が段階的に軽減でき、その結果活性の
平均的な維持が可能となる事、回転ドラム内への固定化
生体触媒の充填により触媒の比表面積を大きくとること
ができ、その結果基質の生成物への転換率が高い事、隔
室前後の回転ドラムの着脱交換あるいは回転ドラム内の
固定化生体触媒の交換により製品の品質が安定する事等
の利点を持っている。``Effects of the Invention'' The present invention uses a rotating drum to widen the scope of application of microorganisms or enzymes to be used, and divides the inside of the reactor tank into several sections and sequentially overflows the substrate solution into the chambers to immobilize the biocatalyst. The load on the substrate can be reduced step by step, making it possible to maintain an average level of activity, and by filling the rotating drum with the immobilized biocatalyst, the specific surface area of the catalyst can be increased, and as a result, the substrate It has the advantages of high conversion rate to products, and product quality can be stabilized by attaching and detaching the rotating drums before and after the compartment, or by replacing the immobilized biocatalyst in the rotating drum.
又各種担体による微生物又は酵素よりなる固定化生体触
媒を充分利用し得るし、
さらに好気性、通性嫌気性何れの発酵にも適用し得る効
果がある。In addition, it is possible to fully utilize immobilized biocatalysts made of microorganisms or enzymes on various carriers, and has the effect of being applicable to both aerobic and facultative anaerobic fermentation.
第１図は本発明の隔室回転ドラム式バイオリアクターを
示す縦断側面図、第２図は上記リアクターの分解斜視図
、第３図は回転ドラムの斜視図である。
１・・横置円筒密閉型槽、８・・反応室、１１・・回転
軸、１５・・有孔回転ドラム。FIG. 1 is a longitudinal sectional side view showing a compartment rotating drum type bioreactor of the present invention, FIG. 2 is an exploded perspective view of the reactor, and FIG. 3 is a perspective view of the rotating drum. 1. Horizontal cylindrical sealed tank, 8. Reaction chamber, 11. Rotating shaft, 15. Perforated rotating drum.
Claims (3)
に回転軸を軸受上で連結する着脱任意で、しかも、固定
化生体触媒を充填保持でき、反応液が容易に出入りでき
る孔を有する回転ドラムを有することを特徴とする隔室
回転ドラム式バイオリアクター。(1) The interior of the horizontal cylindrical closed tank is divided into several reaction chambers, and a rotating shaft is connected to each chamber on a bearing, which can be attached or detached at will.Furthermore, the immobilized biocatalyst can be filled and held, and the reaction solution can be easily dispensed. 1. A compartmental rotating drum bioreactor, characterized in that it has a rotating drum with holes that allow entry and exit.
成繊維、多孔質高分子、多孔質セラミックス等の担体に
包括あるいは架橋、吸着された微生物または酵素よりな
る固定化生体触媒を回転ドラム内に充填する請求項（１
）記載の隔室回転ドラム式バイオリアクター。(2) An immobilized biocatalyst consisting of microorganisms or enzymes enclosed, crosslinked, or adsorbed on a carrier such as natural polymer gel, synthetic polymer gel, natural fiber, synthetic fiber, porous polymer, porous ceramic, etc. in a rotating drum. Claims (1)
) Compartmental rotating drum bioreactor described in ).
換および固定化生体触媒の交換により好気性発酵、通性
嫌気性発酵いずれをも実施可能とする請求項（１）記載
の隔室回転ドラム式バイオリアクター。(3) The spacer according to claim (1), wherein both aerobic fermentation and facultative anaerobic fermentation can be carried out by converting the gases supplied in the tank such as air, oxygen, carbon dioxide, and nitrogen and exchanging the immobilized biocatalyst. Chamber rotating drum bioreactor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63040051A JPH0657146B2 (en) | 1988-02-23 | 1988-02-23 | Compartment rotating drum bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63040051A JPH0657146B2 (en) | 1988-02-23 | 1988-02-23 | Compartment rotating drum bioreactor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01215277A true JPH01215277A (en) | 1989-08-29 |
| JPH0657146B2 JPH0657146B2 (en) | 1994-08-03 |
Family
ID=12570113
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63040051A Expired - Lifetime JPH0657146B2 (en) | 1988-02-23 | 1988-02-23 | Compartment rotating drum bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0657146B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03201975A (en) * | 1989-12-28 | 1991-09-03 | Snow Brand Milk Prod Co Ltd | Sparging method for gas in rotary reactor and system therefor |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5898084A (en) * | 1981-12-02 | 1983-06-10 | Nitto Electric Ind Co Ltd | Reactor containing immobilized enzyme |
| JPS6019598U (en) * | 1983-07-15 | 1985-02-09 | 日産自動車株式会社 | Ram device of cargo handling vehicle |
| JPS626670A (en) * | 1985-07-03 | 1987-01-13 | Japan Synthetic Rubber Co Ltd | Culture device |
-
1988
- 1988-02-23 JP JP63040051A patent/JPH0657146B2/en not_active Expired - Lifetime
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5898084A (en) * | 1981-12-02 | 1983-06-10 | Nitto Electric Ind Co Ltd | Reactor containing immobilized enzyme |
| JPS6019598U (en) * | 1983-07-15 | 1985-02-09 | 日産自動車株式会社 | Ram device of cargo handling vehicle |
| JPS626670A (en) * | 1985-07-03 | 1987-01-13 | Japan Synthetic Rubber Co Ltd | Culture device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03201975A (en) * | 1989-12-28 | 1991-09-03 | Snow Brand Milk Prod Co Ltd | Sparging method for gas in rotary reactor and system therefor |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0657146B2 (en) | 1994-08-03 |
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