JPH0543065B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to a compact device for measuring latex agglutination reaction, which allows quick and reliable testing with simple operations.

[Prior Art] Among immunoserological testing methods, those called indirect agglutination methods include (1) red blood cell agglutination method, (2) latex agglutination method, and (3) cholesterin agglutination method. Of these, (2) the latex agglutination reaction method was first used to test for rheumatoid arthritis, and has since been used to test HCG, rheumatoid factor, hemolytic streptococcus, urinary estrogen, CRP, LE factor, blood
It has been applied to tests for FDP, Toxoplasma, HBs, etc., and is rapidly gaining popularity because its latex reagent has advantages in terms of manufacturing method, specificity, stability, cost, etc., and is easy to handle. It is becoming popular.

The latex reagent used in such a latex agglutination reaction is a sensitized latex reagent made by adsorbing antibodies (or antigens) to latex particles with a particle size of 0.1 to 0.8 μm. (sometimes called latex) detects antigens (or antibodies) in the test solution. In other words, as the antibodies (or antigens) adsorbed on latex particles undergo an antigen-antibody reaction with the corresponding antigens (or antibodies) in the test solution, the latex particles aggregate to form agglomerates. , this may be detected and identified by visual inspection or other appropriate methods. On the other hand, if no antigen (or antibody) is present in the test solution, the latex particles will not aggregate and will still have a milky appearance.

Detects the agglutination reaction of sensitized latex as described above.
Identification methods include (A) spectroscopic turbidity measurement, (B) light scattering, and (C) when the sensitivity of the sensitized latex is high, the aggregation produced as a result of the agglutination reaction. Examples include a method of qualitatively identifying and determining a mass by visual observation. Among them, the method (C) of observing latex agglutination reactions with the naked eye does not require special equipment that is only available in large hospitals or advanced knowledge and techniques that only experts have acquired. It has many advantages and is expected to be widely used. However, this method requires dropping the test solution onto the reaction plate where the latex agglutination reaction is to be observed, dropping the sensitized latex and buffer onto the test solution, stirring them evenly and thoroughly, and using several reaction plates. Not only does it involve very complicated and separate processes, such as observing and determining the state of agglomeration of the latex by gently shaking it for several minutes under illumination, but also the presence or absence of agglomerates produced by the aggregation reaction can be detected within a short period of time (several times). This method has a serious drawback in that if the determination is not made within 1 minute), the reaction solution will evaporate and there is a high possibility that the above determination will be misjudged. For this reason, there has been a demand for a compact apparatus for measuring latex agglutination reactions, which can rationally carry out the above-mentioned independent processes and is less likely to make misjudgments.

[Problems to be Solved by the Invention] The present invention has been made in view of the above-mentioned circumstances, and is a compact device for measuring latex agglutination reaction that can be quickly and reliably tested with simple operations. The aim is to provide equipment.

[Means for Solving the Problems] The device for measuring latex agglutination reaction according to the present invention has a transparent plate and an opaque plate bonded to each other while leaving a reaction storage space between them, and the reaction liquid storage space has an inlet port. It is composed of a mouth, an inner tube, and an outer tube made of an elastic material, and the upper end of the inner tube communicates with the inside of the outer tube, forming a reagent storage space between the inner tube and the outer tube. The gist lies in that the inner tubes of the double-tubular dropper are connected to each other.

[Function] As described above, the device for measuring latex agglutination reaction according to the present invention comprises () pasting together a transparent plate and an opaque plate to form a reaction liquid storage space between them;
The reaction liquid storage space includes an inlet, an inner pipe,
communicating with a double tubular dropper formed of an outer tube made of an elastic material, the upper end of the inner tube communicating with the inside of the outer tube,
() A reagent storage space is formed between the inner tube and the outer tube of the double-tubular dropper, and these (), (),
The greatest feature lies in the synergistic combination of the configurations in parentheses. Below the above (),
The functions and effects of the present invention will be explained while adding explanations for () and ().

() Regarding the fact that a transparent plate and an opaque plate are pasted together to form a reaction storage space between them.By forming a reaction liquid storage space, a place where the reaction liquid can be stored in a sealed state is provided. Therefore, there is no need to prepare a reaction plate as in the conventional method, and there is no need to worry about evaporation or the like even if it takes some time to judge the reaction liquid. By using a transparent plate, it is possible to observe the reaction liquid from the outside, and since an opaque plate is used on the opposite side, it is convenient for highlighting the characteristics of the reaction liquid, such as color and form. Summer.

() Regarding the fact that the reaction liquid storage space communicates with the inlet and the inner tube of the double-tubular dropper.The double-tubular dropper referred to herein is, for example, as shown in FIG. 2. i.e. outer tube 1
5 and an inner tube 14, and the outer tube 15
The inside of the inner tube 14 communicates with the upper end of the inner tube 14, and the outer tube 15 is made of an elastic material (for example, an elastic body), and the fluid is transferred to the outer tube through the inner tube 14 as it deforms. The fluid can be sucked into the reagent storage space 16 inside the reagent 15 and can be stored there, and the stored fluid can also be pushed out through the inner tube 2. Conventionally, by communicating the inner pipe and the suction port of such a double-tube dropper with the reaction liquid storage space and utilizing the action of the double-tube dropper, (a) a reaction plate is prepared; b) It was necessary to separately prepare a dropper independent of the reaction plate to apply quarantine to this, but this is no longer necessary. () Regarding the formation of a reagent storage space between the inner tube and the outer tube of the double-tubular dropper In the above explanation regarding the double-tubular dropper, there is a space in the outer tube 15 and the inner tube 14 that can store fluid. As mentioned above, this space is a space for pre-accommodating and holding reagents, and is also used as a place for mixing, stirring, and reaction with the sample solution inhaled. Become. Moreover, when carrying out the reaction by mixing and stirring, there is no need to take precautions such as gently shaking the reaction plate on which the test solution has been applied, as in the past, so it is easy for the person conducting the test to carry out the reaction. It can be said that the operation has become really simple and easy.

The reaction liquid after the above-mentioned mixing, stirring, and reaction is extruded through the inner tube as shown in the double-tubular dropper, so this extruded reaction liquid is as described in () above. It is moved and stored in the reaction liquid storage space, and is used for determining the reaction results. In other words, even if the mixture is stored in the dropper after mixing, stirring, and reaction, it is not possible to accurately judge the progress of the reaction.

The present invention will be made clearer by describing the embodiments below with reference to the drawings, but the present invention is not limited to the following embodiments, and can be understood within the scope of the present invention based on the preceding and following descriptions. Various modifications are possible. In addition, although the above reagent has been explained by selecting a sensitizing latex, it is not necessary to limit the reagent to this only, and it is possible to sensitize with an antigen (or antibody) that causes an agglutination reaction by introducing a test solution. A color reaction can be observed by introducing a reagent or a test solution such as red blood cells or cholesterin. Needless to say, it is good to use it as a reagent.

[Example] Fig. 1 is an explanatory cross-sectional view showing one embodiment of the present invention, in which a shows a front sectional view and b shows a side sectional view. The latex agglutination reaction measuring device 3 according to the present invention includes a filter section 4, a determination plate 5 and 2.
It is composed of a heavy tubular dropper 6. A filter 8 is filled inside the inhaler 7 in the filter section 4, and a sealing material 8a is laminated to protect the filter 8.
As shown in FIG. 2, it is formed by pasting together a transparent plate 9 and an opaque plate 10 (colored or uncolored) while leaving a reaction liquid storage space 11 between them. And the absorption port 1 of the determination plate 5
2 is fitted with one end of the filter section 4, and the inner circumferential wall of the double tubular dropper side end 13 of the determination plate 5 is fitted with the inner tube 1 of the double tubular dropper 6.
4 is fitted. The outer tube 15 of the double tubular dropper 6 is fitted into the outer peripheral wall of the double tubular dropper side end 13 of the determination plate 5 . A reagent storage space 16 is formed between the inner tube 14 and the outer tube 15 in which a test solution can be stored and a sensitizing latex reagent can be stored therein. The sensitizing latex reagent 17 is stored in advance in the lower part of the space formed between the inner tube 14 and the outer tube 15 of the double-tubular dropper 6, as shown in the figure.

The procedure for using such a device 3 for measuring latex agglutination reaction is as follows, and the explanation will be in bulleted form.

Peel off the seal 8a provided on the filter section 4.

The latex agglutination reaction measuring device 3 is lowered from above the sample liquid storage container, such as a urine collection cup, while maintaining the vertical position shown in FIG. 1 while slightly pressing the outer tube 15 of the double-tubular dropper 6. 4 into the test solution. Next, when the pressure on the outer tube 15 is released, the test liquid passes through the filter section 4 and the reaction liquid storage space 11, rises inside the inner tube 14, and overflows the passage opening at the upper end as shown by the arrow, as shown in FIG. 1b. becomes the state of Note that the filter 8 removes precipitates that may interfere with the reaction and observation of the reaction results.

Immediately after confirming that the amount of the test solution introduced into the reagent storage space 16 has reached a predetermined amount, the device 3 for measuring latex agglutination reaction is pulled up, or the outer tube 15 is pressed again with a finger to remove the inner tube 14. ,
Excess test solution remaining in the reaction solution storage space 11 and filter 8 is removed by dropping.

The sensitized latex 17 is removed by removing the filter part 4 or gently shaking the latex agglutination reaction measuring device 3 with the filter part 4 attached.
Mix and stir the and test solution to react.

After allowing the immunological agglutination reaction to occur for a certain period of time, the reaction solution is transferred to the bottom of the outer tube 15 (the upper end in FIG. 1) with the double tubular dropper 6 facing down, and the outer tube 15 is gently opened again. When pressed with a finger, the transferred reaction liquid is pushed up into the reaction liquid storage space 11.

When the pressure on the outer tube 15 is released, the reaction liquid that has entered the reaction liquid storage space 11 returns to the double tubular dropper 6. Press the tip of the absorption port 12 with your finger or remove the double tubular dropper 6 to equalize the pressure.

The presence or absence of aggregates produced by the immunological agglutination reaction can be observed through the transparent plate 9 against the background of the black opaque plate 10.

Although a black plate was used as the above-mentioned opaque plate, if a white plate is used, it is possible to observe the presence or absence of color development of the chromogen resulting from the immune reaction or enzyme reaction.In this way, the latex agglutination reaction can be observed. In addition to the diagnostic reagent used, it can also be applied as a device for measuring latex agglutination reactions using other chromogens. In any case, the color of the opaque plate is not specified.

[Effects of the Invention] Since the present invention is configured as described above, it is possible to provide a compact device for measuring latex agglutination reaction that can perform tests quickly and reliably with simple operations.

[Brief explanation of the drawing]

FIG. 1 is an explanatory sectional view showing one embodiment of the present invention [a is an explanatory front sectional view, b is an explanatory side sectional view], and FIG. 2 is an explanatory view showing a double tubular dropper. 6...Double tubular dropper, 9...Transparent plate, 10
... Opaque plate, 11 ... Reaction liquid storage space, 12 ...
...Suction port, 14...Inner pipe, 15...Outer pipe, 1
6...Reagent storage space.

Claims (1)

[Scope of Claims] 1. A transparent plate and an opaque plate are bonded together while leaving a reaction liquid storage space between them, and the reaction liquid storage space includes: an inlet, and an inner pipe formed of an elastic material. The inner tube of a double-tubular dropper is composed of an outer tube, the upper end of which is in communication with the inside of the outer tube, and a reagent storage space is formed between the inner tube and the outer tube. A device for measuring latex agglutination reaction, characterized by the following.