JPH06303876A - Method for increasing dissolved oxygen in water - Google Patents
Method for increasing dissolved oxygen in waterInfo
- Publication number
- JPH06303876A JPH06303876A JP9753693A JP9753693A JPH06303876A JP H06303876 A JPH06303876 A JP H06303876A JP 9753693 A JP9753693 A JP 9753693A JP 9753693 A JP9753693 A JP 9753693A JP H06303876 A JPH06303876 A JP H06303876A
- Authority
- JP
- Japan
- Prior art keywords
- water
- dissolved oxygen
- aqueous solution
- oxygen concentration
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Farming Of Fish And Shellfish (AREA)
- Aeration Devices For Treatment Of Activated Polluted Sludge (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は水又は溶解物質を含む水
溶液中の溶存酸素の増加方法に関する。本発明の方法
は、特に、好気性微生物の液体培養あるいは魚類の養殖
に使用される水又は水溶液中の溶存酸素（水中に溶解し
ている酸素）を増加させるのに利用し得る。The present invention relates to a method for increasing dissolved oxygen in water or an aqueous solution containing a dissolved substance. The method of the present invention can be particularly used to increase dissolved oxygen (oxygen dissolved in water) in water or an aqueous solution used for liquid culture of aerobic microorganisms or fish culture.
【０００２】[0002]
【従来の技術及びその解決すべき課題】好気性微生物の
液体培養あるいは魚類の養殖を行う場合には、これらの
目的に使用される水（又は水溶液）中に溶存する酸素の
濃度を高くすることが好ましい。例えば、好気性微生物
を液体培養する場合には、その培養液中の溶存酸素の濃
度を高くすればする程、微生物の増殖が盛んになること
が知られており、そのため、従来から、水中の溶存酸素
の濃度を高めるために種々の方法が行われている。2. Description of the Related Art When liquid culture of aerobic microorganisms or fish culture is carried out, the concentration of oxygen dissolved in water (or aqueous solution) used for these purposes should be increased. Is preferred. For example, when liquid-culturing aerobic microorganisms, it is known that the higher the concentration of dissolved oxygen in the culture solution, the more vigorous the growth of the microorganisms. Various methods have been used to increase the concentration of dissolved oxygen.
【０００３】1 気圧における飽和溶存酸素濃度は約10 m
g/l であり、従って、これ以上の濃度で酸素を水中に包
含させるためには加圧下で酸素を吹き込むしか方法がな
いのが現状である。The saturated dissolved oxygen concentration at 1 atm is about 10 m
It is g / l, and therefore, the only way to make oxygen contained in water at a higher concentration than that is to blow oxygen under pressure.
【０００４】しかしながら、この加圧方式を使用して、
例えば好気性微生物の液体培養を行うための培養液中の
溶存酸素濃度を増加させるためには、培養液を収容する
ための容器として圧力容器を必要とするが、圧力容器は
その製作に費用のかかるものであり、当然、その規模が
制限される。また、培養操作は使用する容器の大きさに
応じたバッチ操作になり、過飽和酸素濃度の水を連続的
に導入し、取出すことは不可能である。従って、例え
ば、安価でかつ溶存酸素濃度の高い水を大量に必要とす
る、好気性微生物を利用する有機汚泥処理に上記したご
とき加圧方式を採用することは物理的にも経済的にも不
可能であった。However, using this pressurization method,
For example, in order to increase the dissolved oxygen concentration in a culture solution for liquid culture of aerobic microorganisms, a pressure vessel is required as a container for containing the culture solution, but the pressure vessel is expensive to manufacture. As such, the size is naturally limited. Further, the culture operation is a batch operation according to the size of the container used, and it is impossible to continuously introduce and remove water having a supersaturated oxygen concentration. Therefore, for example, it is physically and economically unfavorable to adopt the above-mentioned pressure method for treating organic sludge using aerobic microorganisms, which requires a large amount of water that is inexpensive and has a high dissolved oxygen concentration. It was possible.
【０００５】[0005]
【課題を解決するための手段】本発明者は水を大気圧
（１気圧）下で多段に重ねたプレート上を流動させ、循
環させることにより、水中の溶存酸素濃度を飽和溶存酸
素濃度以上に増加させ得ることを知見した。[Means for Solving the Problems] The present inventor has made the dissolved oxygen concentration in water equal to or higher than the saturated dissolved oxygen concentration by causing water to flow under atmospheric pressure (1 atm) on a multi-layered plate and circulating it. It was found that it can be increased.
【０００６】従って、本発明によれば、水又は溶解物質
を含む水溶液を多数の段数のプレート上を流動させかつ
循環させることを特徴とする、水中の溶存酸素の増加方
法が提供される。Therefore, according to the present invention, there is provided a method for increasing dissolved oxygen in water, characterized in that water or an aqueous solution containing a dissolved substance is caused to flow and circulate over a large number of plates.
【０００７】本発明の方法を実施するにあたっては、水
又は水溶液を、通常、その単位容積（１ml）当り、0.3
〜10cm2 、通常、0.35〜5.0 cm2 の表面積になるように
多段に重ねたプレート上を流動させ、循環させる。In carrying out the method of the present invention, water or an aqueous solution is usually added in an amount of 0.3 per unit volume (1 ml).
It is circulated on a multi-tiered plate with a surface area of ~ 10 cm 2 , usually 0.35-5.0 cm 2 , and circulated.
【０００８】前記した通り、１気圧下での水の飽和溶存
酸素濃度は約10 mg/l であり、大気圧下ではこれ以上の
量の酸素を水中に溶解させることは不可能であるとされ
ていた。一方、空気と直接接触する水の表層では上記以
上の酸素を含むこと、即ち酸素を過飽和濃度で含み得る
ことが知られている。本発明者はこの点に着目して、水
の単位容積当りの表面積を増大させるために、水を多段
に重ねたプレート上を流動させることににより、水中の
溶存酸素濃度を飽和溶存酸素濃度以上に増大させること
を可能にしたものである。As described above, the saturated dissolved oxygen concentration of water at 1 atm is about 10 mg / l, and it is considered impossible to dissolve more oxygen in water at atmospheric pressure. Was there. On the other hand, it is known that the surface layer of water that is in direct contact with air contains more oxygen than the above, that is, oxygen can be contained in a supersaturated concentration. The present inventor pays attention to this point, and in order to increase the surface area per unit volume of water, the dissolved oxygen concentration in water is made equal to or higher than the saturated dissolved oxygen concentration by causing water to flow over a multi-layered plate. It is possible to increase to.
【０００９】本発明の方法は図１に示すごとき装置を使
用して実施し得る。図１において、１は水又は水溶液で
ある。水又は水溶液１は貯槽２からポンプ３によりパイ
プ４を経て最上段のプレート５に供給される。水又は水
溶液１は液層１' としてプレート５上を順次流動し、貯
槽２に還送される。貯槽２に還送された水又は水溶液液
１を上記と同様の経路で、必要な回数、循環させる。図
１には３段のプレートが示されているが、プレートの数
は必要に応じて増減させ得る。The method of the present invention may be carried out using an apparatus such as that shown in FIG. In FIG. 1, 1 is water or an aqueous solution. Water or the aqueous solution 1 is supplied from the storage tank 2 to the uppermost plate 5 via the pipe 4 by the pump 3. The water or the aqueous solution 1 sequentially flows as a liquid layer 1 ′ on the plate 5 and is returned to the storage tank 2. The water or aqueous solution 1 returned to the storage tank 2 is circulated as many times as necessary through the same route as above. Although three plates are shown in FIG. 1, the number of plates can be increased or decreased as needed.
【００１０】上記した通り、本発明の方法においては水
又は水溶液を循環させる方式を採用しているため、従来
のエアポンプにより空気を水に吹き込む場合には、水の
容量が増加するにつれてポンプの容量を増加させること
が必要であるのに対し、本発明の方法においてはプレー
トの段数を増加させるだけでよく、従って、操作費用が
著しく低減する。また、液体を循環させているので、液
体が常に均一に攪拌されているいう利点もある。As described above, the method of the present invention employs a method of circulating water or an aqueous solution. Therefore, when air is blown into water by a conventional air pump, the capacity of the pump increases as the capacity of the water increases. While it is necessary to increase the number of plates in the method of the present invention, the operating costs are significantly reduced. Further, since the liquid is circulated, there is an advantage that the liquid is always stirred uniformly.
【００１１】本発明の実施例を以下に示す。Examples of the present invention will be described below.
【００１２】[0012]
【実施例】実施例 縦 10cm 、横 20cm 、高さ 10cm の水槽の上に、縦 10c
m 、横 20cm のプレートを 3〜 10 段、平行に10 cm の
間隔で積み重ね、このプレート上を 4l の水を流速 18
m/分、水温 18 ℃の条件下で流動させ、循環させた。1
分後に、水槽内の溶存酸素濃度を測定した。その結果を
表１に示す。 EXAMPLE vertical 10cm, horizontal 20 cm, on a 10cm height of the tank, the vertical 10c
m, 20 cm wide plates are stacked in 3 to 10 rows in parallel at intervals of 10 cm, and 4 l of water is flown over the plates at a flow rate of 18
It was made to flow and circulated under the condition of m / min and water temperature of 18 ° C. 1
After a minute, the dissolved oxygen concentration in the water tank was measured. The results are shown in Table 1.
【００１３】 [0013]
【００１４】表１の結果を使用して、水の単位容量(ml)
当りの表面積を横軸にとり、溶存酸素濃度を縦軸にとっ
てプロットしたものを図２に示す。図２のグラフから、
水の単位容量当りの表面積が 0.30 cm2 を越えると、そ
の表面積に比例して溶存酸素濃度が増加していることが
明らかである。Using the results in Table 1, unit volume of water (ml)
The surface area per hit is plotted on the horizontal axis and the dissolved oxygen concentration is plotted on the vertical axis, which is shown in FIG. From the graph in Figure 2,
When the surface area per unit volume of water exceeds 0.30 cm 2 , it is clear that the dissolved oxygen concentration increases in proportion to the surface area.
【００１５】また、18℃における水の飽和酸素濃度は9.
18 mg/l であるから、水の単位容量当りの表面積が 0.3
0 cm2 を越えると、水中には飽和酸素濃度以上の酸素が
溶存していることが判る。The saturated oxygen concentration of water at 18 ° C. is 9.
Since it is 18 mg / l, the surface area per unit volume of water is 0.3
When it exceeds 0 cm 2 , it can be seen that oxygen above the saturated oxygen concentration is dissolved in water.
【００１６】上記の方法をスケールアップして、例え
ば、10トンの水に従来の通気攪拌方式に従って酸素の供
給を行った場合には、30馬力のターボブロワーを必要と
するが、本発明に従って酸素の供給を行った場合には、
処理すべき水の容量に応じてプレートの段数を適宜、増
加させるだけでよく、動力としては 1馬力のモーターポ
ンプを必要とするに過ぎない。従って、本発明によれ
ば、動力費用も大幅に削減することが可能である。When the above method is scaled up to supply oxygen to 10 tons of water according to the conventional aeration and stirring method, a turbo blower of 30 hp is required. When the supply of
Depending on the volume of water to be treated, it is only necessary to increase the number of plates in the plate as appropriate, and it only requires a 1hp motor pump. Therefore, according to the present invention, the power cost can be significantly reduced.
【００１７】前記したごとく、本発明の方法は好気性微
生物を液体培養するのに利用し得る。以下にその例を応
用例として示す。As mentioned above, the method of the present invention can be used for liquid culture of aerobic microorganisms. The example is shown below as an application example.
【００１８】応用例 １ 図１に示す装置を使用してパン酵母（菌体濃度 15 g/l
、培養液量 4l ）を培養した。培養時間と菌体濃度の
関係を調べるために、経時的に菌体濃度を測定した。そ
の結果を図３に示す（曲線 A）。菌体濃度の測定は、培
養液を濾過し、その残渣を乾燥した濾紙にはさんで10分
間放置して十分水分を除去した後、その重量を測定する
ことにより行った。 Application Example 1 Using the apparatus shown in FIG. 1, baker's yeast (cell concentration: 15 g / l
, The culture solution volume was 4 l). To examine the relationship between the culture time and the bacterial cell concentration, the bacterial cell concentration was measured over time. The results are shown in Fig. 3 (curve A). The cell concentration was measured by filtering the culture solution, sandwiching the residue between them with a dry filter paper for 10 minutes to sufficiently remove water, and then measuring the weight.
【００１９】比較のため、従来の方法に従って、小型ジ
ャーファーメンターを使用して上記と同一の培養液に殺
菌空気を通気して通気攪拌培養を行い、この場合につい
ても経時的に菌体濃度を測定した。その結果も図３に示
す（曲線 B）。For comparison, according to a conventional method, sterile air was aerated in the same culture solution as described above using a small jar fermenter to perform aeration and agitation culture. It was measured. The results are also shown in Fig. 3 (curve B).
【００２０】図３に示す結果から、従来の通気攪拌培養
（曲線 B）においては培養開始後約14時間で安定増殖期
に入るのに対し、本発明による溶存酸素濃度の増大方法
を利用して行った培養法（曲線 A）においては約 8時間
で安定増殖期に入ることが判る。従って、本発明の溶存
酸素濃度の増大方法を利用した培養法の増殖速度は従来
の方法の約1.8 倍であることが明らかである。また、最
終菌体濃度が曲線 B（従来法）においては70g/l である
のに対し、曲線 A（本発明）においては90g/lであり、
本発明の方法は増殖量においても従来法の約 1.3倍であ
ることが判る。なお、培養液を循環させる時間は増殖さ
せるべき微生物の種類、培養液の菌体濃度、循環させる
培養液の量、循環させる回数等によって変動するが、通
常、10〜72時間程度であろう。From the results shown in FIG. 3, in the conventional aeration and agitation culture (curve B), the stable growth phase is reached about 14 hours after the start of the culture, while the method for increasing the dissolved oxygen concentration according to the present invention is used. It can be seen that the stable growth phase is reached in about 8 hours in the culture method (curve A). Therefore, it is clear that the growth rate of the culture method using the method for increasing the dissolved oxygen concentration of the present invention is about 1.8 times that of the conventional method. Further, the final bacterial cell concentration was 70 g / l in curve B (conventional method), whereas it was 90 g / l in curve A (invention),
It can be seen that the method of the present invention is about 1.3 times as much as the conventional method in terms of the amount of proliferation. The time for circulating the culture solution varies depending on the type of microorganism to be grown, the cell concentration of the culture solution, the amount of the culture solution to be circulated, the number of times of circulation, etc., but it is usually about 10 to 72 hours.
【００２１】応用例 ２ B.O.D.約 50,000 の芋焼酎蒸留廃液 4l を図１に示す装
置の貯槽 2に装入し、活性種汚泥10ccを添加した後、図
１に示す装置内を72時間循環させた。ついで、処理した
廃液にFeCl2 を 100 ppm添加して凝集処理行った後、濾
過した。かく得られた濾液のB.O.D.は約 100 ppmであっ
た。このような結果が得られたのは、芋焼酎蒸留廃液を
本発明の方法に従って処理することにより、廃液中の溶
存酸素濃度が増大し、その結果、廃液中の活性汚泥が急
速に増殖したことによると考えられる。 Application Example 2 4 L of potato shochu distillation waste liquid having a BOD of about 50,000 was charged into the storage tank 2 of the apparatus shown in FIG. . Next, 100 ppm of FeCl 2 was added to the treated waste liquid to perform coagulation treatment, and then filtered. The BOD of the filtrate thus obtained was about 100 ppm. These results were obtained by treating the potato shochu distillation waste liquor according to the method of the present invention to increase the dissolved oxygen concentration in the waste liquor, resulting in rapid growth of the activated sludge in the waste liquor. It is believed that
【図１】本発明の方法の実施に使用する装置である。1 is an apparatus used to carry out the method of the present invention.
【図２】水の単位容量(ml)当りの表面積と、溶存酸素濃
度の関係を示すグラフである。FIG. 2 is a graph showing the relationship between the surface area per unit volume (ml) of water and the dissolved oxygen concentration.
【図３】応用例１に示す方法で得られた、微生物の培養
時間と菌体濃度の関係を示すグラフである。FIG. 3 is a graph showing the relationship between the culture time of microorganisms and the cell concentration obtained by the method shown in Application Example 1.
Claims (1)
数のプレート上を流動させかつ循環させることを特徴と
する、水中の溶存酸素の増加方法。1. A method for increasing dissolved oxygen in water, which comprises causing water or an aqueous solution containing a dissolved substance to flow and circulate over a large number of plates.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9753693A JPH06303876A (en) | 1993-04-23 | 1993-04-23 | Method for increasing dissolved oxygen in water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9753693A JPH06303876A (en) | 1993-04-23 | 1993-04-23 | Method for increasing dissolved oxygen in water |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH06303876A true JPH06303876A (en) | 1994-11-01 |
Family
ID=14194977
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9753693A Pending JPH06303876A (en) | 1993-04-23 | 1993-04-23 | Method for increasing dissolved oxygen in water |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH06303876A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104126539A (en) * | 2014-07-31 | 2014-11-05 | 梁桥锋 | Device for increasing oxygen by making waves through vibration |
| CN105265378A (en) * | 2015-10-28 | 2016-01-27 | 林蓉瑶 | Sea cucumber aquiculture oxygen increasing device |
| CN105955360A (en) * | 2016-06-03 | 2016-09-21 | 厦门海控自动化系统工程有限公司 | Wireless controller and terminal APP control method matched with the same |
| CN109463346A (en) * | 2018-12-28 | 2019-03-15 | 上海海洋大学 | A de-foaming and water-purifying circulation system for temporary maintenance of aquatic products |
-
1993
- 1993-04-23 JP JP9753693A patent/JPH06303876A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104126539A (en) * | 2014-07-31 | 2014-11-05 | 梁桥锋 | Device for increasing oxygen by making waves through vibration |
| CN105265378A (en) * | 2015-10-28 | 2016-01-27 | 林蓉瑶 | Sea cucumber aquiculture oxygen increasing device |
| CN105955360A (en) * | 2016-06-03 | 2016-09-21 | 厦门海控自动化系统工程有限公司 | Wireless controller and terminal APP control method matched with the same |
| CN109463346A (en) * | 2018-12-28 | 2019-03-15 | 上海海洋大学 | A de-foaming and water-purifying circulation system for temporary maintenance of aquatic products |
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