JPH0797978B2 - Soy sauce manufacturing method - Google Patents

Soy sauce manufacturing method

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Publication number
JPH0797978B2
JPH0797978B2 JP4083143A JP8314392A JPH0797978B2 JP H0797978 B2 JPH0797978 B2 JP H0797978B2 JP 4083143 A JP4083143 A JP 4083143A JP 8314392 A JP8314392 A JP 8314392A JP H0797978 B2 JPH0797978 B2 JP H0797978B2
Authority
JP
Japan
Prior art keywords
soy sauce
days
koji
salt
lactic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP4083143A
Other languages
Japanese (ja)
Other versions
JPH05244897A (en
Inventor
英三 北村
卯三夫 江口
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SAITAMA PREFECTURE
Original Assignee
SAITAMA PREFECTURE
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Application filed by SAITAMA PREFECTURE filed Critical SAITAMA PREFECTURE
Priority to JP4083143A priority Critical patent/JPH0797978B2/en
Publication of JPH05244897A publication Critical patent/JPH05244897A/en
Publication of JPH0797978B2 publication Critical patent/JPH0797978B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Description

【発明の詳細な説明】Detailed Description of the Invention

【産業上の利用分野】本発明は、醸造期間を大幅に短縮
した本醸造醤油の製造方法に関する。
BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for producing a brewed soy sauce which has a greatly shortened brewing period.

【従来の技術】醤油の醸造期間は天然醸造で約1年、適
温醸造でも6カ月間を要し、他の食品類の製造期間と比
較して、その製造期間は他に例を見ないほど長い。近
年、醸造期間を短縮する観点から醤油の製造方法が種々
研究され、様々な方法が提案されている。例えば特開昭
61−293368号公報に記載の調味液の製造方法が
提案されている。すなわちこの方法は、醤油製造原料を
通常の方法で処理し、醤油麹菌を接種して、製麹した麹
を、食塩0〜15%(w/v)にして仕込んだもの、ま
たはこれに酵素剤を加えたものから、次の3工程により
調味液を製造する方法である。 第一工程:pH4.0〜9.0及び0〜20℃で1〜7
日間保持。 第二工程:20〜40℃で1〜5日間加水分解。 第三工程:40〜60℃で1〜7日間加水分解。
2. Description of the Related Art Soy sauce brewing takes about one year for natural brewing and six months for moderate temperature brewing. Compared with other foods, the manufacturing period is unprecedented. long. In recent years, various methods for producing soy sauce have been studied from the viewpoint of shortening the brewing period, and various methods have been proposed. For example, a method for producing a seasoning solution described in JP-A-61-293368 has been proposed. That is, this method is one in which the raw material for producing soy sauce is treated by a usual method, inoculated with koji mold of soy sauce, and the koji produced by koji is charged with 0 to 15% (w / v) of salt, or an enzyme agent Is a method for producing a seasoning liquid from the following 3 steps. First step: pH 1 to 7 at pH 4.0 to 9.0 and 0 to 20 ° C.
Hold for days. Second step: hydrolysis at 20-40 ° C for 1-5 days. Third step: hydrolysis at 40-60 ° C for 1-7 days.

【発明が解決しようとする課題】しかしながら、これま
でに提案されている製造期間の大幅に短縮された醤油の
製造方法は、中小企業にとっては、技術や設備等の面か
ら見てその実施が困難であり、現状では採用できるもの
がない。また、従来公知の前記特開昭61−29336
8号公報に記載の方法は、その製造期間は短いが、醤油
の製造に関するものではなく、醤油様の調味液に関する
ものである。この方法により得られた調味液は、調味食
品として利用することも可能であるけれども、香味の優
れた調味液を得るために、さらに乳酸菌や酵母を添加し
て発酵させる等の処理を行うものである。すなわち、依
然として、既存の技術や設備等を使用して、短期間に醤
油を製造する方法は存在しない。本発明はこのような問
題点を解決すべくなされたもので、その目的は、既存の
技術や設備を用いて、醤油の醸造期間を従来より大幅に
短縮した本醸造方式による醤油の製造方法を提供するこ
とにある。
However, it has been difficult for small and medium-sized enterprises to carry out the soy sauce production method proposed so far with a significantly shortened production period from the viewpoint of technology and equipment. Therefore, there is nothing that can be adopted at present. In addition, the previously known JP-A-61-29336 is also known.
Although the method described in Japanese Patent Publication No. 8 has a short production period, it does not relate to the production of soy sauce but to the soy sauce-like seasoning liquid. Although the seasoning solution obtained by this method can be used as a seasoning food, in order to obtain a seasoning solution having an excellent flavor, it is subjected to a treatment such as fermentation by adding lactic acid bacteria or yeast. is there. That is, there is still no method for producing soy sauce in a short period of time by using the existing technology or equipment. The present invention has been made to solve such a problem, and its purpose is to use the existing technology and equipment to provide a method for producing soy sauce by the present brewing method in which the brewing period of soy sauce is significantly shortened compared to the conventional method. To provide.

【課題を解決するための手段】前記目的を達成するため
に、本発明の醤油の製造方法は、通常の方法で製麹した
醤油麹に、食塩濃度が〜10%(w/v)になるよう
に食塩水を加えて得た混合物を、10〜15℃で15〜
20日間保持し、次いで45〜50℃で3〜4日間消化
し、さらに食塩を加えて塩分濃度を15〜20%(w/
v)に調整した後、35℃以下で乳酸菌及び酵母を接種
して30〜40日間発酵・熟成させることを特徴とす
る。本発明において使用する醤油麹は、通常の方法によ
り製造されるもの、すなわち原料として蛋白質原料と澱
粉質原料をそれぞれ変性、α化等の処理を行ない混合し
たものに、通常の醤油用麹菌を加えて、35℃以下主に
25℃〜33℃で3〜4日間機械製麹したものである。
これらの代表例は3日麹あるいは4日麹である。このよ
うにして製麹した醤油麹に食塩水を加え、7〜10%
(w/v)、好ましくは7〜8%(w/v)の食塩濃度
になるように仕込んで諸味とする。食塩濃度は低い方が
グルタミン酸の生成に有利であるが、食塩濃度が
(w/v)に満たないと雑菌に汚染されやすくなる。1
0%(w/v)を越えると、グルタミン酸の生成が不十
分となり窒素溶解利用率も低下する。次の諸味を消化す
る工程においては、まず10〜15℃の低温で15〜2
0日間保持する。この低温で消化する工程において、温
度が10℃に満たないと、グルタミン酸の生成が不十分
となり、15℃を越えると、雑菌に汚染されやすくな
る。低温で消化する期間は長いほど好ましいが、次の高
温での消化において種々の酵素によるグルタミン酸の生
成要因にも配慮する必要がある。次に、45〜50℃好
ましくは45〜47℃の高温で、3〜4日間消化する。
温度が45℃に満たないと雑菌に汚染されやすくなり、
50℃を越えるとグルタミン酸の生成が低下し、また分
解臭も強くなる。消化を完了させるためには3〜4日間
が必要である。すなわち、醤油の呈味成分であるグルタ
ミン酸を増加させるためには、高温で消化する前に、低
い食塩濃度で低温で消化する必要がある。消化完了後、
さらに食塩を加えて塩分濃度を15〜20%(w/v)
好ましくは16〜18%(w/v)に調整する。すなわ
ち、市販の醤油の塩分濃度になるように食塩を補給する
必要がある。塩分濃度が低いと、雑菌による汚染や異常
発酵のおそれがある。乳酸菌及び酵母は、35℃以下好
ましくは25〜30℃で同時に接種し、30〜40日間
発酵・熟成させて本醸造醤油を製造する。乳酸菌と酵母
を同時に添加する本発明の方法は、諸味pHを監視する
必要がなく簡便である。前記温度が35℃を越えると、
乳酸菌の活性が低下する。発酵、熟成を完了させるには
30〜40日間必要である。醤油の醸造において、乳酸
発酵及びアルコール発酵は呈味バランスの上からも不可
欠であるので、本発明方法の発酵・熟成工程には乳酸菌
と酵母の併用が必須である。酵母としては、主発酵酵母
であるZygosaccharomycesrouxi
等だけでなく、醤油の香気を生成させるために、Ca
ndidaversatilis等の後熟酵母を併用す
る。発酵・熟成の完了後、通常の方法のとおり、圧搾、
火入れそしてロ過を行って製品を製造する。
In order to achieve the above-mentioned object, the method for producing soy sauce of the present invention is such that soy sauce koji produced by a conventional method has a salt concentration of 7 to 10% (w / v). The mixture obtained by adding saline to the mixture at 10 to 15 ° C.
Hold for 20 days, then digest at 45-50 ° C for 3-4 days , and add salt to adjust salt concentration to 15-20% (w /
After adjusting to v), it is characterized by inoculating lactic acid bacteria and yeast at 35 ° C. or lower and fermenting and aging for 30 to 40 days. The soy sauce koji used in the present invention is produced by an ordinary method, that is, a protein raw material and a starch raw material as raw materials are respectively modified, mixed with each other after undergoing treatment such as gelatinization, and ordinary soy sauce koji mold is added. Then, the koji was mechanically kneaded at a temperature of 35 ° C or lower, mainly at 25 ° C to 33 ° C for 3 to 4 days.
Typical examples of these are 3-day koji or 4-day koji. Pressure to give a saline soy sauce koji koji Thus, 7-10%
(W / v), favored properly to the moromi were charged so that the salt concentration of 7~8% (w / v). A lower salt concentration is more advantageous for the production of glutamic acid, but a salt concentration of 7 %
If it is less than (w / v), it is likely to be contaminated with various bacteria. 1
If it exceeds 0% (w / v), the production of glutamic acid becomes insufficient and the nitrogen dissolution utilization rate also decreases. In the next step of digesting moromi, first, at a low temperature of 10 to 15 ° C.
Hold for 0 days. In this step of digesting at a low temperature, if the temperature is lower than 10 ° C, the production of glutamic acid becomes insufficient, and if it exceeds 15 ° C, it is easily contaminated with various bacteria. The longer the digestion period at a low temperature, the more preferable, but it is also necessary to take into consideration factors for producing glutamic acid by various enzymes in the subsequent digestion at a high temperature. Next, it is digested at a high temperature of 45 to 50 ° C, preferably 45 to 47 ° C for 3 to 4 days.
If the temperature is less than 45 ° C, it will be easily contaminated by various bacteria,
If the temperature exceeds 50 ° C, the production of glutamic acid will decrease and the decomposition odor will also increase. To complete the digestion Ru required der is 3-4 days <br/>. That is, in order to increase glutamic acid, which is a taste component of soy sauce, it is necessary to digest at low temperature with low salt concentration before digesting at high temperature. After the digestion is complete,
Add salt to adjust the salt concentration to 15-20% (w / v)
It is preferably adjusted to 16 to 18% (w / v). That is, it is necessary to supplement the salt so that the salt concentration of commercially available soy sauce is reached. If the salt concentration is low, there is a risk of contamination by various bacteria and abnormal fermentation. Lactic acid bacteria and yeast are simultaneously inoculated at 35 ° C or lower, preferably 25 to 30 ° C, and fermented and aged for 30 to 40 days to produce the brewed soy sauce. The method of the present invention in which lactic acid bacteria and yeast are added at the same time is simple without the need to monitor the moromi pH. If the temperature exceeds 35 ° C,
The activity of lactic acid bacteria is reduced. It takes 30 to 40 days to complete fermentation and ripening. In brewing soy sauce, lactic acid fermentation and alcoholic fermentation are indispensable from the viewpoint of taste balance, and therefore, the combined use of lactic acid bacteria and yeast is indispensable in the fermentation / ripening process of the method of the present invention. As yeast, Zygosaccharomyces rouxi which is the main fermentation yeast
In order to generate aroma of soy sauce as well as i , Ca
Post- ripening yeast such as Ndida satilis is also used. After completion of fermentation and aging, press, as usual,
The product is manufactured by firing and filtering.

【作用】醤油の呈味主成分はグルタミン酸であり、その
多少は醤油の品質を左右する。醤油原料中の全グルタミ
ン酸の約46%は、グルタミンとして存在していると推
定される。グルタミンは麹のグルタミナーゼにより分解
されて、グルタミン酸が生成する。グルタミナーゼは耐
熱性、耐塩性が弱いことから、麹を高温で消化すると、
グルタミナーゼ活性は低下し、グルタミンは速やかに呈
味性のないピログルタミン酸に変化するので、旨味の少
ない醤油になると考えられる。本発明の方法では、低塩
下で低温消化することにより、グルタミンからのグルタ
ミン酸の生成を促進させた後、低塩かつ高温下で雑菌の
増殖を防ぎつつ消化することにより、麹の他の酵素活性
を高め、窒素利用率の高い消化諸味を得ることができ
る。さらに、発酵工程において、本発明の方法では諸味
が高温を経ているため、天然醸造で行われているような
麹由来の乳酸菌及び酵母による発酵は期待できず、乳酸
菌の添加も必須となる。一方、高温消化により、乳酸菌
の栄養源となる糖類が充分生成しており、雑菌も大幅に
減少しているので、乳酸菌の添加効果が上がることとな
り、発酵速度も速まる。
[Operation] The main component of the taste of soy sauce is glutamic acid, and the amount of soy sauce affects the quality of soy sauce. It is estimated that about 46% of all glutamic acid in the soy sauce raw material exists as glutamine. Glutamine is decomposed by koji glutaminase to produce glutamic acid. Glutaminase has poor heat resistance and salt resistance, so when koji is digested at high temperature,
The glutaminase activity decreases, and glutamine rapidly changes to non-tasting pyroglutamic acid, which is considered to be soy sauce with less umami. In the method of the present invention, by low temperature digestion under low salt, after promoting the production of glutamic acid from glutamine, by digesting while preventing the growth of miscellaneous bacteria under low salt and high temperature, other enzymes of koji. The activity can be enhanced and digestive moromi with high nitrogen utilization can be obtained. Furthermore, in the fermentation process, since the moromi tastes at a high temperature in the method of the present invention, fermentation with lactic acid bacteria and yeast derived from koji as in natural brewing cannot be expected, and addition of lactic acid bacteria is also essential. On the other hand, by the high temperature digestion, saccharides, which are the nutrient source of lactic acid bacteria, are sufficiently produced, and various bacteria are significantly reduced. Therefore, the effect of adding lactic acid bacteria is enhanced and the fermentation rate is also increased.

【発明の効果】以上詳しく説明した通り、本発明によ
り、既存の技術や設備を利用して、原料利用率及びグル
タミン酸生成を向上させ、醤油の醸造期間を従来より大
幅に短縮した、本醸造方式による醤油の製造方法を提供
することが可能となった。そのため、醤油製造の回転が
速まり、コストの低減化並びに需要に対し迅速に対応す
ることができる。
As described in detail above, according to the present invention, the existing technique and equipment are used to improve the utilization rate of raw materials and the production of glutamic acid, and the brewing period of soy sauce is greatly shortened as compared with the conventional method. It has become possible to provide a method for producing soy sauce according to. Therefore, the rotation of soy sauce production is accelerated, the cost can be reduced, and the demand can be promptly dealt with.

【実施例】以下に、実施例により、本発明をさらに詳細
に説明する。脱脂加工大豆と小麦との等量配合原料を3
5℃以下(主に25〜33℃)で機械製麹した3日麹4
5.3kgに、予め5℃に冷却した10.6%(w/
v)の食塩水79.9リットルを加えて(10℃)、塩
分濃度7.98%(w/v)で仕込を行なった。この混
合物を、1日1回攪拌しながら、10℃で15日間消化
した。次いで、45℃まで加温して、同温度で3日間、
温度の均一化のため2時間に1回の割合で空気攪拌を行
って、消化した。これに食塩濃度が16〜18%(w/
v)になるように補塩後、諸味pH5.5付近で、初発
pHが低くても増殖旺盛な乳酸菌(Pediococc
us halophilus F30)及び酵母(Zy
gosaccharomycesrouxii F13
及びCandida versatilis T12)
をそれぞれ諸味1gに対して10、10、10
ベルとなるよう添加し、28〜30℃で発酵・熟成させ
た。乳酸菌及び酵母の添加後10日間は、5日に1回、
その後は2日に1回攪拌を行なった。菌を添加して40
日後に、常法通り圧搾、火入れ及びロ過を行って、本醸
造醤油を製造した。圧搾後の生醤油を、醤油試験法に準
じて成分分析等を行なった。その結果、全窒素1.59
%、ホルモール窒素0.94%、食塩16.4%、還元
糖2.58%、アルコール2.28%、無塩可溶性固形
分18.3%、pH4.89、色度27、グルタミン酸
1.45%、ピログルタミン酸0.29%、乳酸0.6
7%、酢酸0.16%、4−エチルグアヤコール2.3
ppm、4−エチルフェノール0.2ppm、グルタミ
ン酸/T.N.値0.91、窒素溶解利用率89.5%
であり、窒素溶解利用率が高くグルタミン酸生成量の多
い本醸造醤油を約2か月で製造することができた。
EXAMPLES The present invention will be described in more detail below with reference to examples. 3 defatted soybeans and 3 equivalents of wheat
Machine-made koji at 5 ℃ or below (mainly 25-33 ℃)
10.6% (w /
79.9 liters of the saline solution of v) was added (10 ° C.), and the mixture was charged at a salt concentration of 7.98% (w / v). The mixture was digested at 10 ° C for 15 days with stirring once a day. Then, heat to 45 ° C. and keep at the same temperature for 3 days,
To homogenize the temperature, air digestion was performed once every 2 hours for digestion. The salt concentration is 16-18% (w /
v) After supplementing with salt, the lactic acid bacterium ( Pediococc), which has strong growth even at a low initial pH, is around Moromi pH 5.5.
us halophilus F30) and yeast ( Zy
gosaccharomyces rouxii F13
And Candida versatilis T12)
Were each added so as to be 10 6, 10 5, 10 5 level for moromi 1g, fermented and ripening at 28 to 30 ° C.. For 10 days after addition of lactic acid bacteria and yeast, once every 5 days,
After that, the mixture was stirred once every two days. 40 by adding bacteria
After the day, squeezing, burning and filtering were carried out as usual to produce the brewed soy sauce. The raw soy sauce after pressing was subjected to component analysis and the like according to the soy sauce test method. As a result, total nitrogen 1.59
%, Formol nitrogen 0.94%, common salt 16.4%, reducing sugar 2.58%, alcohol 2.28%, salt-free soluble solid content 18.3%, pH 4.89, chromaticity 27, glutamic acid 1.45. %, Pyroglutamic acid 0.29%, lactic acid 0.6
7%, acetic acid 0.16%, 4-ethyl guaiacol 2.3
ppm, 4-ethylphenol 0.2 ppm, glutamic acid / T. N. Value 0.91, nitrogen dissolution utilization rate 89.5%
Therefore, the brewed soy sauce having a high nitrogen dissolution utilization rate and a large amount of glutamic acid produced could be produced in about 2 months.

───────────────────────────────────────────────────── フロントページの続き (56)参考文献 特開 昭61−293368(JP,A) 特開 昭49−132299(JP,A) 特開 昭50−94196(JP,A) 特開 昭52−18897(JP,A) 特開 昭52−76488(JP,A) 特開 昭48−87090(JP,A) 特公 昭49−15797(JP,B1) ─────────────────────────────────────────────────── ─── Continuation of the front page (56) References JP-A 61-293368 (JP, A) JP-A 49-132299 (JP, A) JP-A 50-94196 (JP, A) JP-A 52- 18897 (JP, A) JP 52-76488 (JP, A) JP 48-87090 (JP, A) JP 49-15797 (JP, B1)

Claims (1)

【特許請求の範囲】[Claims] 【請求項1】 通常の方法で製麹した醤油麹に、食塩濃
度が〜10%(w/v)になるように食塩水を加えて
得た混合物を、10〜15℃で15〜20日間保持し、
次いで45〜50℃で3〜4日間消化し、さらに食塩を
加えて塩分濃度を15〜20%(w/v)に調整した
後、35℃以下で乳酸菌及び酵母を接種して30〜40
日間発酵・熟成させること、を特徴とする醤油の製造方
法。
1. A mixture obtained by adding a saline solution to a soy sauce koji produced by a conventional method so that the salt concentration is 7 to 10% (w / v), and the mixture is prepared at 10 to 15 ° C. for 15 to 20. Hold for days,
Then, the mixture is digested at 45 to 50 ° C for 3 to 4 days , and salt is further added to adjust the salt concentration to 15 to 20% (w / v), and then lactic acid bacteria and yeast are inoculated at 35 ° C or lower to 30 to 40%.
A method for producing soy sauce, which comprises fermenting and aging for days.
JP4083143A 1992-03-05 1992-03-05 Soy sauce manufacturing method Expired - Lifetime JPH0797978B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP4083143A JPH0797978B2 (en) 1992-03-05 1992-03-05 Soy sauce manufacturing method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP4083143A JPH0797978B2 (en) 1992-03-05 1992-03-05 Soy sauce manufacturing method

Publications (2)

Publication Number Publication Date
JPH05244897A JPH05244897A (en) 1993-09-24
JPH0797978B2 true JPH0797978B2 (en) 1995-10-25

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JP (1) JPH0797978B2 (en)

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