JPH1082784A5 - - Google Patents

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Publication number
JPH1082784A5
JPH1082784A5 JP1997205458A JP20545897A JPH1082784A5 JP H1082784 A5 JPH1082784 A5 JP H1082784A5 JP 1997205458 A JP1997205458 A JP 1997205458A JP 20545897 A JP20545897 A JP 20545897A JP H1082784 A5 JPH1082784 A5 JP H1082784A5
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Japan
Prior art keywords
hyaluronic acid
antibody
shap
iti
binding
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JP1997205458A
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Japanese (ja)
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JPH1082784A (en
JP3976848B2 (en
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Priority to JP20545897A priority Critical patent/JP3976848B2/en
Priority claimed from JP20545897A external-priority patent/JP3976848B2/en
Publication of JPH1082784A publication Critical patent/JPH1082784A/en
Publication of JPH1082784A5 publication Critical patent/JPH1082784A5/ja
Application granted granted Critical
Publication of JP3976848B2 publication Critical patent/JP3976848B2/en
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Expired - Fee Related legal-status Critical Current

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Claims (12)

検体中の血清由来ヒアルロナン結合性タンパク質−ヒアルロン酸（ＳＨＡＰ−ＨＡ）結合体を、抗インター−α−トリプシンインヒビター抗体（抗ＩＴＩ抗体）と反応させて抗ＩＴＩ抗体−（ＳＨＡＰ−ＨＡ結合体）の複合体を形成させ、この複合体を測定することを特徴とする検体中の血清由来ヒアルロナン結合性タンパク質−ヒアルロン酸結合体の測定方法。A method for measuring serum-derived hyaluronan-binding protein-hyaluronic acid (SHAP-HA) conjugates in a sample, characterized by reacting serum-derived hyaluronan-binding protein-hyaluronic acid (SHAP-HA) conjugates in the sample with anti-inter-α-trypsin inhibitor antibody (anti-ITI antibody) to form an anti-ITI antibody-(SHAP-HA conjugate) complex, and measuring this complex. 検体中のＳＨＡＰ−ＨＡ結合体と、ヒアルロン酸と結合能を有するタンパク質とを反応させてヒアルロン酸と結合能を有するタンパク質とＳＨＡＰ−ＨＡ結合体との複合体を形成させ、この複合体を抗ＩＴＩ抗体と反応させて、抗ＩＴＩ抗体−（ＳＨＡＰ−ＨＡ結合体）−（ヒアルロン酸と結合能を有するタンパク質）の複合体を形成させ、この複合体を測定することを特徴とする検体中の血清由来ヒアルロナン結合性タンパク質−ヒアルロン酸結合体の測定方法。A method for measuring serum-derived hyaluronan-binding protein-hyaluronic acid conjugates in a sample, comprising reacting a SHAP-HA conjugate in the sample with a protein capable of binding to hyaluronic acid to form a complex between the protein capable of binding to hyaluronic acid and the SHAP-HA conjugate, reacting this complex with an anti-ITI antibody to form a complex of anti-ITI antibody-(SHAP-HA conjugate)-(protein capable of binding to hyaluronic acid), and measuring this complex. ヒアルロン酸と結合能を有するタンパク質がヒアルロン酸結合性タンパク質およびヒアルロン酸受容体から選択される少なくとも１種のタンパク質である請求項２記載の測定方法。3. The method according to claim 2, wherein the protein capable of binding to hyaluronic acid is at least one protein selected from the group consisting of hyaluronic acid-binding proteins and hyaluronic acid receptors. ヒアルロン酸結合性タンパク質がヒアルロン酸結合性プロテオグリカン、ヒアルロン酸結合性プロテオグリカンのコアタンパク質、リンクプロティン、ヒアルロネクチンおよびそれらタンパク質のヒアルロン酸結合領域を含む部分タンパク質よりなる群から選択される少なくとも１種のタンパク質である請求項３記載の測定方法。The measurement method according to claim 3, wherein the hyaluronic acid-binding protein is at least one protein selected from the group consisting of hyaluronic acid-binding proteoglycans, core proteins of hyaluronic acid-binding proteoglycans, link proteins, hyaluronectins, and partial proteins containing the hyaluronic acid-binding domains of these proteins. ヒアルロン酸受容体がＣＤ４４、そのヒアルロン酸結合領域を含む部分タンパク質またはそれらの融合タンパク質である請求項３記載の測定方法。The method according to claim 3, wherein the hyaluronic acid receptor is CD44, a partial protein containing its hyaluronic acid-binding domain, or a fusion protein thereof. 抗ＩＴＩ抗体がＩＴＩの長鎖である HC1および／またはHC2と反応するポリクローナル抗体またはモノクローナル抗体である請求項１〜３のいずれかに記載の測定方法。4. The method according to claim 1, wherein the anti-ITI antibody is a polyclonal or monoclonal antibody that reacts with HC1 and/or HC2, which are the long chains of ITI. キットの構成成分として、抗ＩＴＩ抗体を含むことを特徴とし、検体中のＳＨＡＰ−ＨＡ結合体と抗ＩＴＩ抗体とを結合させて抗ＩＴＩ抗体−（ＳＨＡＰ−ＨＡ結合体）の複合体を形成させ、ＳＨＡＰ−ＨＡ結合体を測定する方法に使用するための測定キット。A measurement kit characterized by including an anti-ITI antibody as a component of the kit, which is used in a method for measuring SHAP-HA conjugates by binding the SHAP-HA conjugates in a sample with the anti-ITI antibody to form an anti-ITI antibody-(SHAP-HA conjugate) complex. 抗ＩＴＩ抗体がＩＴＩの長鎖である HC1および／またはHC2と反応するポリクローナル抗体またはモノクローナル抗体である請求項７記載の測定キット。8. The assay kit according to claim 7, wherein the anti-ITI antibody is a polyclonal or monoclonal antibody that reacts with HC1 and/or HC2, which are the long chains of ITI. 請求項１〜６のいずれかの測定方法を用いて検体中のＳＨＡＰ−ＨＡ結合体を測定し、それによって関節炎を検出することを特徴とする関節炎の検出方法。A method for detecting arthritis, comprising measuring SHAP-HA conjugates in a sample using the measurement method of any one of claims 1 to 6, thereby detecting arthritis . 請求項１〜６のいずれかの測定方法を用いて検体中のＳＨＡＰ−ＨＡ結合体を測定し、それによって肝疾患を検出することを特徴とする肝疾患の検出方法。A method for detecting liver disease, comprising measuring SHAP-HA conjugates in a sample using the measurement method of any one of claims 1 to 6, thereby detecting liver disease. 検体が血液、血清または血漿である請求項９又は１０記載の検出方法。11. The detection method according to claim 9 or 10, wherein the sample is blood, serum or plasma. キットの構成成分として抗ＩＴＩ抗体を含み、検体中のＳＨＡＰ−ＨＡ結合体と抗ＩＴＩ抗体とを結合させて抗ＩＴＩ抗体−（ＳＨＡＰ−ＨＡ結合体）の複合体を形成させ、ＳＨＡＰ−ＨＡ結合体を測定することを特徴とする関節炎又は肝疾患の診断用キット。A diagnostic kit for arthritis or liver disease, which comprises an anti-ITI antibody as a component of the kit, binds a SHAP-HA conjugate in a sample with the anti-ITI antibody to form an anti-ITI antibody-(SHAP-HA conjugate) complex, and measures the SHAP-HA conjugate.
JP20545897A 1996-07-16 1997-07-15 Method and kit for measuring serum-derived hyaluronan binding protein-hyaluronic acid conjugate Expired - Fee Related JP3976848B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP20545897A JP3976848B2 (en) 1996-07-16 1997-07-15 Method and kit for measuring serum-derived hyaluronan binding protein-hyaluronic acid conjugate

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP8-205282 1996-07-16
JP20528296 1996-07-16
JP20545897A JP3976848B2 (en) 1996-07-16 1997-07-15 Method and kit for measuring serum-derived hyaluronan binding protein-hyaluronic acid conjugate

Related Child Applications (2)

Application Number Title Priority Date Filing Date
JP2006010713A Division JP4217716B2 (en) 1996-07-16 2006-01-19 Method and kit for measuring serum-derived hyaluronan-binding protein-hyaluronic acid conjugate
JP2006010714A Division JP4217717B2 (en) 1996-07-16 2006-01-19 Method and kit for measuring serum-derived hyaluronan-binding protein-hyaluronic acid conjugate

Publications (3)

Publication Number Publication Date
JPH1082784A JPH1082784A (en) 1998-03-31
JPH1082784A5 true JPH1082784A5 (en) 2005-02-10
JP3976848B2 JP3976848B2 (en) 2007-09-19

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JP20545897A Expired - Fee Related JP3976848B2 (en) 1996-07-16 1997-07-15 Method and kit for measuring serum-derived hyaluronan binding protein-hyaluronic acid conjugate

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JP (1) JP3976848B2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1059087A1 (en) * 1999-06-08 2000-12-13 Peter Prof. Dr. Prehm Antigens from rheumatoid autoimmune diseases
WO2000074705A1 (en) * 1999-06-08 2000-12-14 Peter Prehm Rheumatic autoimmune disease antigen
US20070259380A1 (en) * 2004-05-20 2007-11-08 Kyoichi Sumida Method for Measuring Hyaluronic Acid Using Hyaluronic Acid Binding Protein
JP2006250862A (en) * 2005-03-14 2006-09-21 Seikagaku Kogyo Co Ltd Method for and kit for detecting pregnancy toxemia
US7812125B2 (en) * 2007-03-27 2010-10-12 Anamar Medical Ab Cartilage intermediate layer protein 2 C1 and its use to differentiate osteoarthritis from rheumatoid arthritis and non-disease conditions
US11730811B2 (en) 2017-10-05 2023-08-22 Kaohsiung Medical University Method for treating subject suffering from flavivirus infection
TWI651535B (en) * 2017-10-05 2019-02-21 Kaohsiung Medical University Evaluation of the severity patients with dengus virus infection by blood hyaluronan levels

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