JPS6410983A - Artificial tissue and production thereof - Google Patents
Artificial tissue and production thereofInfo
- Publication number
- JPS6410983A JPS6410983A JP63026050A JP2605088A JPS6410983A JP S6410983 A JPS6410983 A JP S6410983A JP 63026050 A JP63026050 A JP 63026050A JP 2605088 A JP2605088 A JP 2605088A JP S6410983 A JPS6410983 A JP S6410983A
- Authority
- JP
- Japan
- Prior art keywords
- artificial
- tissue
- connective tissue
- ascorbic acid
- phosphoric ester
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000001519 tissue Anatomy 0.000 abstract 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 abstract 4
- 210000002808 connective tissue Anatomy 0.000 abstract 4
- 150000002148 esters Chemical class 0.000 abstract 3
- 229960005070 ascorbic acid Drugs 0.000 abstract 2
- 235000010323 ascorbic acid Nutrition 0.000 abstract 2
- 239000011668 ascorbic acid Substances 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 abstract 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 abstract 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 abstract 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 abstract 1
- 210000004102 animal cell Anatomy 0.000 abstract 1
- 239000003242 anti bacterial agent Substances 0.000 abstract 1
- 229940088710 antibiotic agent Drugs 0.000 abstract 1
- 239000002473 artificial blood Substances 0.000 abstract 1
- 210000004204 blood vessel Anatomy 0.000 abstract 1
- 210000000988 bone and bone Anatomy 0.000 abstract 1
- 210000003321 cartilage cell Anatomy 0.000 abstract 1
- 210000002889 endothelial cell Anatomy 0.000 abstract 1
- 210000002744 extracellular matrix Anatomy 0.000 abstract 1
- 210000002950 fibroblast Anatomy 0.000 abstract 1
- 210000004185 liver Anatomy 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
- 210000000329 smooth muscle myocyte Anatomy 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Materials For Medical Uses (AREA)
Abstract
PURPOSE:To obtain artificial tissue useful as artificial skin, artificial blood vessel, artificial bone or artificial liver, by cultivating cells for connective tissue in an ascorbic acid phosphoric ester-containing medium and forming tissues consisting of the connective tissue and ECM. CONSTITUTION:Cells of connective tissue such as fibroblast, smooth muscle cell, cartilage cell or endothelial cell is cultivated by stationery culture in a medium for animal cell containing animal serum and antibiotics and 0.01-5mM, preferably 0.05-0.4mM ascorbic acid phosphoric ester such as ascorbic acid-2- phosphoric ester at 35-38 deg.C at pH 7-7.4 for >=3 weeks and about 4 months to form tissue consisting of connective tissue and ECM (extracellular matrix component). The prepared artificial tissue is formed in a sheetlike state on the container wall of a culture container. The artificial tissue is directly used as it is and optionally separated into a sheetlike state by using EDTA, etc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63026050A JPS6410983A (en) | 1987-02-19 | 1988-02-05 | Artificial tissue and production thereof |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3758587 | 1987-02-19 | ||
| JP63026050A JPS6410983A (en) | 1987-02-19 | 1988-02-05 | Artificial tissue and production thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6410983A true JPS6410983A (en) | 1989-01-13 |
Family
ID=26363784
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63026050A Pending JPS6410983A (en) | 1987-02-19 | 1988-02-05 | Artificial tissue and production thereof |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6410983A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0750664A4 (en) * | 1994-02-25 | 1999-01-13 | Univ California | REPRODUCTION AND USE OF FUNCTIONAL HUMAN TISSUE |
| JP2006034346A (en) * | 2004-07-22 | 2006-02-09 | Olympus Corp | Endoscope apparatus |
| JP2010187680A (en) * | 1998-11-19 | 2010-09-02 | Organogenesis Inc | Bioengineered tissue construct and method for producing and using the same |
| JP4699453B2 (en) * | 2005-04-05 | 2011-06-08 | オリンパスメディカルシステムズ株式会社 | Connector for fluid supply device, fluid supply device |
| JP4856060B2 (en) * | 2005-04-05 | 2012-01-18 | オリンパスメディカルシステムズ株式会社 | Endoscope system |
-
1988
- 1988-02-05 JP JP63026050A patent/JPS6410983A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0750664A4 (en) * | 1994-02-25 | 1999-01-13 | Univ California | REPRODUCTION AND USE OF FUNCTIONAL HUMAN TISSUE |
| JP2010187680A (en) * | 1998-11-19 | 2010-09-02 | Organogenesis Inc | Bioengineered tissue construct and method for producing and using the same |
| JP2006034346A (en) * | 2004-07-22 | 2006-02-09 | Olympus Corp | Endoscope apparatus |
| JP4699453B2 (en) * | 2005-04-05 | 2011-06-08 | オリンパスメディカルシステムズ株式会社 | Connector for fluid supply device, fluid supply device |
| JP4856060B2 (en) * | 2005-04-05 | 2012-01-18 | オリンパスメディカルシステムズ株式会社 | Endoscope system |
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