NL2017209A - Test strip for double-antibody sandwich colloidal golddetection against human lactoferrin - Google Patents

Test strip for double-antibody sandwich colloidal golddetection against human lactoferrin Download PDF

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Publication number
NL2017209A
NL2017209A NL2017209A NL2017209A NL2017209A NL 2017209 A NL2017209 A NL 2017209A NL 2017209 A NL2017209 A NL 2017209A NL 2017209 A NL2017209 A NL 2017209A NL 2017209 A NL2017209 A NL 2017209A
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gold
test strip
monoclonal antibody
colloidal gold
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NL2017209A
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English (en)
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NL2017209B1 (en
Inventor
Wang Qin
Lin Xiangmei
Liu Xiaofei
Feng Chunyan
Fu Wei
Li Xiaolin
Qiu Songyin
Liu Dandan
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Chinese Acad Inspection & Quarantine
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Publication of NL2017209B1 publication Critical patent/NL2017209B1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/539—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody involving precipitating reagent, e.g. ammonium sulfate
    • G01N33/541—Double or second antibody, i.e. precipitating antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/79—Transferrins, e.g. lactoferrins, ovotransferrins

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)

Claims (5)

1. Teststrip voor dubbel-antilichaam sandwich colloïdale gouddetectie tegen humaan lactoferrine, de betreffende teststrip omvattende een waterabsorptiepad, een basisfilm, een goud-gelabelde pad en een monsterpad die sequentieel verbonden zijn, waarbij een lijn C en een lijn T gepositioneerd zijn op de basisfilm, de lijn C gecoat is met een antilichaam geit anti-muis IgG, en de goud-gelabelde pad een eerste monoklonaal antilichaam bevat gelabeld met colloïdaal goud; de lijn T gecoat is met een tweede monoklonaal antilichaam; het eerste monoklonaal antilichaam uitgescheiden is door een hybridomacellijn met het depotnummer CGMCC NO. 10595, en het tweede monoklonaal antilichaam uitgescheiden is door een hybridomacellijn met het depotnummer CGMCC NO. 10594.
2. Teststrip voor dubbel-antilichaam sandwich colloïdale gouddetectie tegen humaan lactoferrine volgens conclusie 1, waarbij het eerste monoklonaal antilichaam dat gelabeld is met colloïdaal goud, dat zit in de goud-gelabelde pad, verkregen is door het sproeien van een oplossing bevattend 5-100 pg/mL van het eerste monoklonaal antilichaam dat gelabeld is met colloïdaal goud, bij de sproeisnelheid van 0.5-5 pL/cm op de goud-gelabelde pad door middel van een goud-sproeiende fïlmdoseermachine en vervolgens het drogen van de goud-gelabelde pad.
3. Teststrip voor dubbel-antilichaam sandwich colloïdale gouddetectie tegen humaan lactoferrine volgens conclusie 1, waarbij het tweede monoklonaal antilichaam gecoat op de lijn T verkregen is door een oplossing te sproeien die 0.2-5 mg/mL van het tweede monoklonaal antilichaam bevat, bij de sproeisnelheid van 0.2-4 pL/cm op de lijn T door middel van een goud-sproeiende fïlmdoseermachine en vervolgens het drogen van de lijn T.
4.
Teststrip voor dubbel-antilichaam sandwich colloïdale gouddetectie tegen humaan lactoferrine volgens conclusie 1, waarbij het geit anti-muis IgG gecoat op de lijn C verkregen is door een oplossing te sproeien die 0.2-5 mg/mL van het geit anti-muis IgG antilichaam bevat, bij de sproeisnelheid van 0.2-4 pL/cm op de lijn C door middel van een goud-sproeiende fïlmdoseermachine en vervolgens het drogen van de lijn C.
NL2017209A 2015-07-30 2016-07-22 Test strip for double-antibody sandwich colloidal golddetection against human lactoferrin NL2017209B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510459441.2A CN105021830B (zh) 2015-07-30 2015-07-30 一种人乳铁蛋白双抗夹心胶体金检测试纸条

Publications (2)

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NL2017209A true NL2017209A (en) 2017-02-02
NL2017209B1 NL2017209B1 (en) 2017-05-23

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NL2017209A NL2017209B1 (en) 2015-07-30 2016-07-22 Test strip for double-antibody sandwich colloidal golddetection against human lactoferrin

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NL (1) NL2017209B1 (nl)

Cited By (2)

* Cited by examiner, † Cited by third party
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CN107561072A (zh) * 2017-09-14 2018-01-09 深圳泰首智能技术有限公司 精确实现puc‑t测试结果的判读方法及其判读装置
NL2019595B1 (en) * 2017-06-16 2018-12-24 Chinese Acad Inspection & Quarantine Monoclonal antibody against nucleocapsid proteins of channel catfish virus and application thereof

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CN105717308A (zh) * 2016-03-24 2016-06-29 泰州海路生物技术有限公司 快速定量检测粪便乳铁蛋白的免疫层析试剂盒
CN109336973B (zh) * 2018-10-11 2021-08-24 中国科学院昆明动物研究所 抗转铁蛋白抗体及其用途
CN110196330A (zh) * 2019-03-22 2019-09-03 新疆大学 一种检测骆驼乳中掺伪牛乳的免疫胶体金试纸条及其应用
CN110068688B (zh) * 2019-05-22 2020-08-14 福建农林大学 一种牛乳中乳铁蛋白竞争法纳米花免疫测流层析检测卡
CN110244062A (zh) * 2019-07-18 2019-09-17 珠海市医友生物科技有限公司 一种粪便钙卫蛋白或乳铁蛋白联合检测试剂及其制备方法
CN111896749A (zh) * 2020-08-07 2020-11-06 杭州都林生物科技有限公司 牛乳铁蛋白双抗体夹心胶体金免疫层析试纸条、制备方法、试剂盒和检测方法
WO2023180899A1 (en) * 2022-03-21 2023-09-28 Swain Manish Kumar Device and method for detecting diarrhea

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Publication number Priority date Publication date Assignee Title
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CN107561072A (zh) * 2017-09-14 2018-01-09 深圳泰首智能技术有限公司 精确实现puc‑t测试结果的判读方法及其判读装置
CN107561072B (zh) * 2017-09-14 2020-02-11 深圳泰首智能技术有限公司 精确实现puc-t测试结果的判读方法及其判读装置

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CN105021830A (zh) 2015-11-04
CN105021830B (zh) 2017-03-15

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