NO169573B - PROCEDURE FOR ENSILING GROENFOR, APPLYING MICRO-ORGANISMS TO THIS, AND BIOLOGICALLY CLEAN CULTURES OF THE ORGANISMS - Google Patents
PROCEDURE FOR ENSILING GROENFOR, APPLYING MICRO-ORGANISMS TO THIS, AND BIOLOGICALLY CLEAN CULTURES OF THE ORGANISMS Download PDFInfo
- Publication number
- NO169573B NO169573B NO871988A NO871988A NO169573B NO 169573 B NO169573 B NO 169573B NO 871988 A NO871988 A NO 871988A NO 871988 A NO871988 A NO 871988A NO 169573 B NO169573 B NO 169573B
- Authority
- NO
- Norway
- Prior art keywords
- lactobacillus plantarum
- organisms
- dsm
- ensiling
- plantarum dsm
- Prior art date
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
- A23K30/10—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder
- A23K30/15—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging
- A23K30/18—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging using microorganisms or enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Virology (AREA)
- General Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Animal Husbandry (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Storage Of Fruits Or Vegetables (AREA)
Abstract
Description
Oppfinnelsen vedrører en fremgangsmåte for ensilering av grønnfor, anvendelse av mikroorganismer til dette, samt biologisk rene kulturer av organismene. The invention relates to a method for ensiling green fodder, the use of microorganisms for this, as well as biologically pure cultures of the organisms.
Ensileringen av grønnfor har overordentlig økonomisk betyd-ning, og det er foreslått forskjellige fremgangsmåter resp. tilsetningsstoffer til akselering av gjæring av grønnfor-masser resp. til forbedring av ensilasjene, innbefattende tilsetningen av melkesyrefrembringende bakterier, da melke-syregjæringen er en vesentlig prosess ved ensilering av grønnfor. The silage of green fodder is extremely economically important, and various methods have been proposed, resp. additives for accelerating the fermentation of green mass resp. for the improvement of the silages, including the addition of lactic acid-producing bacteria, as the lactic acid fermentation is an essential process when ensiling green fodder.
En god ensilasje bør ved slutten av gjæringen inneholde 2 til 3 vekt-# melkesyre. De tilsatte bakterier bør kunne forgjære alle eller flest mulig av de i grønnforet inneholdte sukkere. Denne oppgavestilling løses imidlertid ikke tilstrekkelig av de kjente bakterier, som man hittil har tilsatt ensilasjene (sammenlign AT-A 262 040). At the end of fermentation, a good silage should contain 2 to 3 wt-# of lactic acid. The added bacteria should be able to ferment all or most of the sugars contained in the green feed. However, this task is not sufficiently solved by the known bacteria, which have been added to the silages up to now (compare AT-A 262 040).
Til grunn for oppfinnelsen ligger den oppgave å tilveiebringe bakteriepreparater resp. bakterier som gir forbedrede ensilasjer, spesielt bevirker i kortere tid en sterkere surgjøring av ensilasjen, idet smørsyregjæringen best mulig unngås. The invention is based on the task of providing bacterial preparations or bacteria that provide improved silages, in particular cause a stronger acidification of the silage in a shorter time, as butyric acid fermentation is best avoided.
Oppfinnelsens gjenstand er følgelig en fremgangsmåte til ensilering av grønnfor og anvendelse av bakteriepreparater av melkesyrefrembringende bakterier, idet fremgangsmåten er kjennetegnet ved at det anvendes Lactobacillus plantarum DSM 3676 og/eller Lactobacillus plantarum DSM 3677. The object of the invention is therefore a method for ensiling green fodder and using bacterial preparations of lactic acid-producing bacteria, the method being characterized by the use of Lactobacillus plantarum DSM 3676 and/or Lactobacillus plantarum DSM 3677.
Det ble overraskende funnet at de to mikroorganismer DSM 3676 og DSM 3677, når de anvendes sammen, har en kompletterende virkning. Det er derfor foretrukket at det anvendes en blanding av, referert til antall av kimer, 10 til 90% Lactobacillus plantarum DSM 3676 og 90 til 10$ Lactobacillus plantarum DSM 3677. Spesielt foretrukket er en blanding av 30 til 70% DSM 3676 og 70 til 30% DSM 3677, spesielt 40 til 60$ DSM 3676 og 60 til 40% DSM 3677. It was surprisingly found that the two microorganisms DSM 3676 and DSM 3677, when used together, have a complementary effect. It is therefore preferred that a mixture of, referred to the number of germs, 10 to 90% Lactobacillus plantarum DSM 3676 and 90 to 10% Lactobacillus plantarum DSM 3677 is used. Particularly preferred is a mixture of 30 to 70% DSM 3676 and 70 to 30% DSM 3677, especially 40 to 60$ DSM 3676 and 60 to 40% DSM 3677.
Oppfinnelsen vedrører også anvendelsen av Lactobacillus plantarum DSM 3676 og/eller Lactobacillus plantarum DSM 3677 til ensilering av grønnfor. The invention also relates to the use of Lactobacillus plantarum DSM 3676 and/or Lactobacillus plantarum DSM 3677 for ensiling green fodder.
Oppfinnelsen vedrører også biologisk rene kulturer av Lactobacillus plantarum som er kjennetegnet ved at kulturen er Lactobacillus plantarum DSM 3676. Den vedrører også biologisk rene kulturer av Lactobacillus plantarum som er kjennetegnet ved at kulturen er Lactobacillus plantarum DSM 3677. The invention also relates to biologically pure cultures of Lactobacillus plantarum characterized by the fact that the culture is Lactobacillus plantarum DSM 3676. It also relates to biologically pure cultures of Lactobacillus plantarum characterized by the culture being Lactobacillus plantarum DSM 3677.
De to ovennevnte ifølge oppfinnelsen anvendte mikroorganismer er tidligere ikke omtalt. Den ene av disse mikroorganismer ble av søkeren først gitt betegnelsen B 5 og deponert 12.3.86 ved Deutsche Sammlung von Mikroorganismen, Grisebachstr. 8, D-3400 Gottingen, og fikk nr. DSM 3676. (Deponering ifølge Budapest-avtalen vedrørende den internasjonale anerkjennelse av deponering av mikroorganismer for patentfremgangsmåter.) The two above-mentioned microorganisms used according to the invention have not previously been mentioned. One of these microorganisms was first given the designation B 5 by the applicant and deposited on 12.3.86 at the Deutsche Sammlung von Mikroorganismen, Grisebachstr. 8, D-3400 Gottingen, and received No. DSM 3676. (Deposit according to the Budapest Agreement concerning the International Recognition of the Deposit of Microorganisms for Patent Processes.)
Den andre mikrorganisme fikk av søkeren først betegnelsen W 2 og likeledes deponert 12.3.86 ved samme deponeringssted. Den fikk nr. DSM 3677. The second micro-organism was first given the designation W 2 by the applicant and likewise deposited on 12.3.86 at the same deposition site. It was given the No. DSM 3677.
Begge mikroorganismer ble identifisert som Lactobacillus plantarum, Subspecies arabinosus. De har følgende egen-skaper : Both microorganisms were identified as Lactobacillus plantarum, Subspecies arabinosus. They have the following properties:
Ingen gassdannelse No gas formation
DL-melkesyre DL-lactic acid
Ingen ammoniakk fra arginin No ammonia from arginine
Temperaturoptimum ca. 35"C Temperature optimum approx. 35"C
Ingen vekst ved 45°C No growth at 45°C
Vekst ved 15°C Growth at 15°C
Celleveggbestanddel: Cell wall component:
Diaminopimelinsyre Diaminopimelic acid
Sukkerforgjæring Sugar fermentation
Glukose positiv Mannitol positiv Glycerol negativ Arabinose positiv Sakkarose positiv Glucose positive Mannitol positive Glycerol negative Arabinose positive Sucrose positive
Laktose positiv Melibiose positiv Dekstrin positiv (ved W 2) Lactose positive Melibiose positive Dextrin positive (at W 2)
(ved B 5 svakere) (at B 5 weaker)
Stivelse negativ Starch negative
Eskulin positiv Esculin positive
Maltose positiv Melizitose positiv Maltose positive Melizitose positive
Xylose negativ Xylose negative
Ribose positiv Trihalose positiv Raffinose positiv Sorbitol positiv Ribose positive Trihalose positive Raffinose positive Sorbitol positive
Salicin positiv Salicin positive
Ramnose positiv (ved W 2) Rhamnose positive (at W 2)
(ved B 5 negativ) (at B 5 negative)
Forsk. i eller: Research. in or:
W 2 danner slim W 2 forms mucus
B 5 danner intet slim B 5 forms no mucus
Acetatforenlighet: Acetate Compatibility:
For W 2 er 1,5$ For W 2 is 1.5$
For B 5 er 0, 5% For B 5 is 0.5%
Morfologi. Morphology.
W 2 = enkelte småstaver W 2 = some lowercase letters
B 5 = kortstaver i kjeder B 5 = short letters in chains
Syring i MRS: Acidification in MRS:
Ved B 5 3,81 pH At B 5 3.81 pH
Ved W 2 3,79 pH At W 2 3.79 pH
Stoffskifte bestemt for B 5: Av 1 mol glukose blandes 2 mol laktat og av 1 mol dripose blandes 1 mol laktat + 0,65 mol acetat og etanol i spor. Metabolism determined for B 5: Of 1 mol of glucose, 2 mol of lactate is mixed and of 1 mol of drip bag, 1 mol of lactate + 0.65 mol of acetate and ethanol are mixed in traces.
Mikroorganismene kan dyrkes på vanlig måte. Egnede dyrk-ningsbetingelser i MRS-medium: The microorganisms can be grown in the usual way. Suitable cultivation conditions in MRS medium:
pH før sterilisering: 6,9 pH before sterilization: 6.9
Sterilisering 20 minutter ved 121°C Sterilization 20 minutes at 121°C
pH etter sterilisering: 6,6 pH after sterilization: 6.6
Forhold ovenfor oksygen: Conditions above oxygen:
Mikroaereofil (+) Microaerophile (+)
På konserveringsagar ved kjøleskapoppbevaring. On preservation agar for refrigerated storage.
Dyrkningstemperatur: 25 til 32<*>C Cultivation temperature: 25 to 32<*>C
Dyrkningsvarighet: 24 timer Cultivation duration: 24 hours
Overpodningsintervall: Ca. 3 måneder Overgrafting interval: Approx. 3 months
Egnede oppbevaringsbetingelser: Suitable storage conditions:
a) Stikk-kultur i konserveringsagar (som vanlig for Lacto-baciller) a) Stick culture in preservation agar (as usual for Lactobacilli)
Etter oppvekst, oppbevaring i kjøleskap. After growth, store in a refrigerator.
b) Frysetørkede kulturer ved -20°C omtrent ubegrenset lagringsdyktig. b) Freeze-dried cultures at -20°C can be stored approximately indefinitely.
Betingelser for prøving av levedyktighet: Conditions for testing viability:
Platestøp eller stikk-kultur i MRS-medier. Plate cast or stick culture in MRS media.
De nye mikroorganismer utmerker seg ved spesielle evner i intensitet og hastighet ved fermentering av sukkere, slik de foreligger i grønnfor ved de vanlige ensileringstemperaturer. Mikroorganismene ble isolert fra grønnforensilasjer i tidlig gjæringsfase. The new microorganisms are distinguished by special abilities in intensity and speed when fermenting sugars, as they are present in green fodder at the usual ensiling temperatures. The microorganisms were isolated from green forage silages in the early fermentation phase.
I følgende tabeller er det angitt resultatene som ble oppnådd ved ensilering av gress resp. lucern under anvendelse av mikroorganismen ifølge oppfinnelsen, samt ensileringsmidler i henhold til teknikkens stand. Jo lavere pH-verdien er, desto sterkere er den oppnådde ensilering og desto bedre er dette for ensilasjen. The following tables show the results obtained by ensiling grass or lucerne using the microorganism according to the invention, as well as silage agents according to the state of the art. The lower the pH value, the stronger the silage achieved and the better this is for the silage.
I tabellen er det anvendt følgende forkortelser: The following abbreviations are used in the table:
TS: Tørrstoff TS: Dry matter
"Kofa-Lac" handelspreparat i henhold til teknikkens stand "Kofa-Lac" commercial preparation according to the state of the art
"Kofa-Plus" handelspreparat i henhold til teknikkens stand på "Kofa-Plus" commercial preparation according to the state of the art
basis av kjemikalier basis of chemicals
Tallene IO<5> resp. 10^ angir antall kimer pr. 1 ml resp. 1 g frisk masse (podningsstyrke) The numbers IO<5> resp. 10^ indicates the number of germs per 1 ml or 1 g of fresh pulp (graft strength)
Usteril: Usteril Unsterile: Unsterile
"Biomax" handelspreparat i henhold til teknikkens stand De i tabellen angitte tall er de respektive pH-verdier. "Biomax" commercial preparation according to the state of the art The numbers given in the table are the respective pH values.
Dyrkingen av bakteriene kan foregå på i og for seg kjent måte (sammenlign AT-A-262 040). Under dyrkingen kan det eventuelt til næringsmediet settes et alkalisk virkende stoff for ikke å la pH-verdien synke for sterkt. Eksempler på slike alkaliske stoffer er kaliumhydroksydoppløsning, natrium-hydroksydoppløsning, sodaoppløsning. Atskillelsen av cellene kan foregå på kjent måte. Eksempelvis kan cellene atskilles i en sentrifuge. Man kan deretter sette til dem et beskyt-telsesstoff, eksempelvis et beskyttelseskolloid, f.eks. melkepulver. Det kan også anvendes andre beskyttelsesstoffer, eksempelvis laktose, ascorbinsyre. The cultivation of the bacteria can take place in a manner known per se (compare AT-A-262 040). During cultivation, an alkaline-acting substance can possibly be added to the nutrient medium in order not to let the pH drop too much. Examples of such alkaline substances are potassium hydroxide solution, sodium hydroxide solution, soda solution. The separation of the cells can take place in a known manner. For example, the cells can be separated in a centrifuge. You can then add a protective substance to them, for example a protective colloid, e.g. powdered milk. Other protective substances can also be used, for example lactose, ascorbic acid.
Cellene kan da frysetørkes. Man får et stabilt preparat som inneholder de aktive bakterier og er holdbart i lang tid uten tap i aktivitet. The cells can then be freeze-dried. You get a stable preparation that contains the active bacteria and is durable for a long time without loss of activity.
Preparatet kan settes til godset som skal ensileres på kjent måte for hånden, doseringsapparat, pulversprøyter og lign-ende . The preparation can be added to the goods to be ensiled in a known manner by hand, dosing device, powder syringes and the like.
Claims (6)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19863616181 DE3616181A1 (en) | 1986-05-14 | 1986-05-14 | PROCESS FOR ACIDIFYING GREEN FORAGE |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| NO871988D0 NO871988D0 (en) | 1987-05-13 |
| NO871988L NO871988L (en) | 1987-11-16 |
| NO169573B true NO169573B (en) | 1992-04-06 |
| NO169573C NO169573C (en) | 1992-07-15 |
Family
ID=6300784
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO871988A NO169573C (en) | 1986-05-14 | 1987-05-13 | PROCEDURE FOR ENSILING GROENFOR, APPLYING MICRO-ORGANISMS TO THIS, AND BIOLOGICALLY CLEAN CULTURES OF THE ORGANISMS |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0250786B1 (en) |
| AT (1) | ATE80978T1 (en) |
| DE (2) | DE3616181A1 (en) |
| IE (1) | IE59968B1 (en) |
| NO (1) | NO169573C (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI82354C (en) * | 1988-11-14 | 1991-03-11 | Valio Meijerien | KONSERVERING AV FAERSKFODER. |
| DE3916563A1 (en) * | 1989-05-20 | 1990-11-22 | Atochem Werke Gmbh | COMBINATION DEVICE AND METHOD FOR ACIDIFYING GREEN FORAGE AND PREVENTING AEROBIC DEGRADING PROCESSES IN GAERFUTTER |
| DE4034749C2 (en) * | 1990-11-01 | 2002-11-07 | Addcon Agrar Gmbh | Combination preparation and method for acidifying green fodder and preventing aerobic degradation processes in fermented fodder |
| DE4112866A1 (en) * | 1991-04-19 | 1992-10-22 | Sanofi Ceva Gmbh | Inhibiting Listeria in fermented fodder, esp. silage - by adding alkali salt of sulphurous acid and nitrite giving synergistic effect |
| NZ591040A (en) * | 2008-07-21 | 2012-08-31 | Erber Ag | Method for treating feed silage for ruminants by treatment with at least two microbes selected from specific Lactobacillus, Enterococcus, Trichosporon strains |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT262040B (en) * | 1964-07-13 | 1968-05-27 | Theodor Dr Beck | Process for the production of a lactic acid bacterial preparation |
| IT1109471B (en) * | 1976-08-17 | 1985-12-16 | Deral Sa | PROCEDURE AND PRODUCT FOR THE PRESERVATION AND ENHANCEMENT OF GREEN VEGETABLES AND OF THE WET PRODUCTS UNDER AGRO-FOOD INDUSTRIES |
| US4528199A (en) * | 1983-01-26 | 1985-07-09 | University Of Georgia Research Foundation, Inc. | Silage production from fermentable forages |
| FR2542013B1 (en) * | 1983-03-01 | 1986-01-03 | Abc Bio Ind | PROCESS FOR THE PREPARATION AND STORAGE OF A PROTEIN HYDROLYSAT USEFUL IN PARTICULAR IN THE AGRI-FOOD FIELD |
-
1986
- 1986-05-14 DE DE19863616181 patent/DE3616181A1/en not_active Withdrawn
-
1987
- 1987-05-07 EP EP87106658A patent/EP0250786B1/en not_active Expired - Lifetime
- 1987-05-07 DE DE8787106658T patent/DE3781943D1/en not_active Expired - Lifetime
- 1987-05-07 AT AT87106658T patent/ATE80978T1/en active
- 1987-05-13 IE IE124787A patent/IE59968B1/en not_active IP Right Cessation
- 1987-05-13 NO NO871988A patent/NO169573C/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| NO871988L (en) | 1987-11-16 |
| EP0250786B1 (en) | 1992-09-30 |
| NO871988D0 (en) | 1987-05-13 |
| NO169573C (en) | 1992-07-15 |
| EP0250786A3 (en) | 1989-07-05 |
| IE871247L (en) | 1987-11-14 |
| EP0250786A2 (en) | 1988-01-07 |
| DE3616181A1 (en) | 1987-11-26 |
| ATE80978T1 (en) | 1992-10-15 |
| IE59968B1 (en) | 1994-05-04 |
| DE3781943D1 (en) | 1992-11-05 |
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