NO20022351L - System og fremgangsmÕte for manipulering av magnetisk pÕvirkbare partikler i fluidpröver for Õ samle opp DNA eller RNAfra en pröve - Google Patents

System og fremgangsmÕte for manipulering av magnetisk pÕvirkbare partikler i fluidpröver for Õ samle opp DNA eller RNAfra en pröve

Info

Publication number
NO20022351L
NO20022351L NO20022351A NO20022351A NO20022351L NO 20022351 L NO20022351 L NO 20022351L NO 20022351 A NO20022351 A NO 20022351A NO 20022351 A NO20022351 A NO 20022351A NO 20022351 L NO20022351 L NO 20022351L
Authority
NO
Norway
Prior art keywords
particles
solution
nucleic acid
acid molecules
magnetically responsive
Prior art date
Application number
NO20022351A
Other languages
English (en)
Other versions
NO330113B1 (no
NO20022351D0 (no
Inventor
Timothy Roy Hansen
Bradley Scott Thomas
John Joseph Bianco
Matthew P Collis
Original Assignee
Beckton Dickinson And Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckton Dickinson And Company filed Critical Beckton Dickinson And Company
Publication of NO20022351D0 publication Critical patent/NO20022351D0/no
Publication of NO20022351L publication Critical patent/NO20022351L/no
Publication of NO330113B1 publication Critical patent/NO330113B1/no

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B03SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03CMAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00Magnetic separation
    • B03C1/02Magnetic separation acting directly on the substance being separated
    • B03C1/28Magnetic plugs and dipsticks
    • B03C1/288Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B03SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03CMAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00Details of magnetic or electrostatic separation
    • B03C2201/26Details of magnetic or electrostatic separation for use in medical or biological applications
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027General features of the devices
    • G01N2035/103General features of the devices using disposable tips
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0099Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/028Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Clinical Laboratory Science (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Et system og fremgangsmåte for manipulering av magnetisk responsive partikler i en løsning for å separere nukleinsyremolekyler fra cellekomponenter i en celleløsning. Systemet og fremgangsmåten anvender en anordning som er i stand til å motta flere rør, hvert av disse inneholder respektive prøver og magnetisk responsive partikler. Anordningen innbefatter oppvarmings- og avkjølingsinnretninger for å lettgjøre et ingtrinn for å frigjøre nukleinsyremolekyler fra cellene i celleløsningen. Anordningen innbefatter videre bevegelige magneter som kan beveges nær til og vekk fra rørene som holder de magnetisk responsive partiklene til hvilke nukleinsyremolekylene blir bundet, slik at molekylbundne partikler kan separeres fra resten av løsningen og vaskes, slik det er hensiktsmessig. Systemet anvender også en elektromagnet som er i stand til å demagnetisere partiklene for å tillate partiklene fritt å blandes med løsningen, slik som elusjonsløsninger som anvendes for å frigjøre molekylene fra partiklene.
NO20022351A 2001-05-17 2002-05-16 System og fremgangsmate for manipulering av magnetisk pavirkbare partikler i fluidprover for a samle opp DNA eller RNA fra en prove NO330113B1 (no)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/858,889 US6672458B2 (en) 2000-05-19 2001-05-17 System and method for manipulating magnetically responsive particles fluid samples to collect DNA or RNA from a sample

Publications (3)

Publication Number Publication Date
NO20022351D0 NO20022351D0 (no) 2002-05-16
NO20022351L true NO20022351L (no) 2002-11-18
NO330113B1 NO330113B1 (no) 2011-02-21

Family

ID=25329438

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20022351A NO330113B1 (no) 2001-05-17 2002-05-16 System og fremgangsmate for manipulering av magnetisk pavirkbare partikler i fluidprover for a samle opp DNA eller RNA fra en prove

Country Status (8)

Country Link
US (2) US6672458B2 (no)
EP (1) EP1260583A1 (no)
JP (1) JP3676754B2 (no)
AU (1) AU785010B2 (no)
BR (1) BR0201515B1 (no)
CA (1) CA2380919C (no)
FI (1) FI119552B (no)
NO (1) NO330113B1 (no)

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NO330113B1 (no) 2011-02-21
JP3676754B2 (ja) 2005-07-27
US20020014443A1 (en) 2002-02-07
EP1260583A1 (en) 2002-11-27
FI20020923A0 (fi) 2002-05-16
BR0201515A (pt) 2003-02-11
AU4059102A (en) 2002-11-21
CA2380919A1 (en) 2002-11-17
FI119552B (fi) 2008-12-31
NO20022351D0 (no) 2002-05-16
CA2380919C (en) 2011-09-27
BR0201515B1 (pt) 2013-08-06
US20030038071A1 (en) 2003-02-27
FI20020923L (fi) 2002-11-18
AU785010B2 (en) 2006-08-24
US6672458B2 (en) 2004-01-06

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