NO329246B1 - Fremgangsmate for fremstilling av proteiner ved autoproteolytisk spalting ved bruk av ekspresjonsvektor og bakterievertscelle - Google Patents

Fremgangsmate for fremstilling av proteiner ved autoproteolytisk spalting ved bruk av ekspresjonsvektor og bakterievertscelle Download PDF

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Publication number
NO329246B1
NO329246B1 NO20020507A NO20020507A NO329246B1 NO 329246 B1 NO329246 B1 NO 329246B1 NO 20020507 A NO20020507 A NO 20020507A NO 20020507 A NO20020507 A NO 20020507A NO 329246 B1 NO329246 B1 NO 329246B1
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NO
Norway
Prior art keywords
polypeptide
pro
expression vector
host cell
fusion protein
Prior art date
Application number
NO20020507A
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English (en)
Norwegian (no)
Other versions
NO20020507L (no
NO20020507D0 (no
Inventor
Tillmann Rumenapf
Heinz-Jurgen Thiel
Jorg Windisch
Franz Knauseder
Original Assignee
Sandoz Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sandoz Ag filed Critical Sandoz Ag
Publication of NO20020507D0 publication Critical patent/NO20020507D0/no
Publication of NO20020507L publication Critical patent/NO20020507L/no
Publication of NO329246B1 publication Critical patent/NO329246B1/no

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
NO20020507A 1999-08-09 2002-01-31 Fremgangsmate for fremstilling av proteiner ved autoproteolytisk spalting ved bruk av ekspresjonsvektor og bakterievertscelle NO329246B1 (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT136899 1999-08-09
PCT/EP2000/007642 WO2001011056A1 (en) 1999-08-09 2000-08-07 Production of proteins by autoproteolytic cleavage

Publications (3)

Publication Number Publication Date
NO20020507D0 NO20020507D0 (no) 2002-01-31
NO20020507L NO20020507L (no) 2002-04-05
NO329246B1 true NO329246B1 (no) 2010-09-20

Family

ID=3512383

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20020507A NO329246B1 (no) 1999-08-09 2002-01-31 Fremgangsmate for fremstilling av proteiner ved autoproteolytisk spalting ved bruk av ekspresjonsvektor og bakterievertscelle

Country Status (17)

Country Link
US (1) US7378512B2 (cs)
EP (1) EP1200603B1 (cs)
JP (1) JP5480458B2 (cs)
KR (1) KR100735791B1 (cs)
CN (1) CN1230542C (cs)
AU (1) AU775681B2 (cs)
CA (1) CA2380302C (cs)
CZ (1) CZ303341B6 (cs)
HK (1) HK1047127B (cs)
HU (1) HU230117B1 (cs)
IL (2) IL147411A0 (cs)
MX (1) MXPA02001425A (cs)
NO (1) NO329246B1 (cs)
PL (1) PL203708B1 (cs)
SK (1) SK288290B6 (cs)
TR (1) TR200200048T2 (cs)
WO (1) WO2001011056A1 (cs)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2823220B1 (fr) 2001-04-04 2003-12-12 Genodyssee Nouveaux polynucleotides et polypeptides de l'erythropoietine (epo)
CA2467397A1 (en) * 2001-11-26 2003-06-05 Replikun Biotech Pty Ltd Flavivirus vaccine delivery system
AU2011253661B2 (en) * 2005-04-26 2013-06-13 Boehringer Ingelheim Rcv Gmbh & Co Kg Production of recombinant proteins by autoproteolytic cleavage of a fusion protein
WO2006113959A2 (en) * 2005-04-26 2006-11-02 Sandoz Ag Production of recombinant proteins by autoproteolytic cleavage of a fusion protein
GB0508435D0 (en) * 2005-04-26 2005-06-01 Sandoz Ag Organic compounds
GB0508434D0 (en) * 2005-04-26 2005-06-01 Sandoz Ag Organic compounds
US20100137563A1 (en) 2008-12-03 2010-06-03 Northwestern University Cysteine Protease Autoprocessing of Fusion Proteins
EP2684951A1 (en) 2012-07-13 2014-01-15 Sandoz Ag Method for producing a recombinant protein of interest
EP2746390A1 (en) * 2012-12-19 2014-06-25 Sandoz Ag Method for producing a recombinant protein of interest
EP2746391A1 (en) * 2012-12-19 2014-06-25 Sandoz Ag Method for producing a recombinant protein of interest
UY35874A (es) 2013-12-12 2015-07-31 Novartis Ag Un proceso para la preparación de una composición de proteínas pegiladas
CN117362401A (zh) * 2023-09-27 2024-01-09 中国农业科学院兰州兽医研究所 一种牛病毒性腹泻病毒n蛋白及其作为自我切割蛋白的应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4743679A (en) * 1986-02-24 1988-05-10 Creative Biomolecules, Inc. Process for producing human epidermal growth factor and analogs thereof
DE3885431D1 (de) * 1987-12-23 1993-12-09 Boehringer Ingelheim Int Expression der viral kodierten Protease P2A des HRV2.
US6077694A (en) * 1990-09-21 2000-06-20 The United States Of America As Represented By The Department Of Health And Human Services Method for over-expression and rapid purification of biosynthetic proteins
DE69836075T2 (de) * 1997-04-25 2007-06-06 SemBioSys Genetics, Inc., Calgary Verfahren zur spaltung von fusionproteinen
AU9260598A (en) * 1997-08-22 1999-03-16 Boehringer Mannheim Gmbh Zymogenic protease precursors that can be autocatalytically activated and their use
WO1999010483A2 (de) * 1997-08-22 1999-03-04 Roche Diagnostics Gmbh Autokatalytisch aktivierbare vorstufen von proteasen und deren verwendung
KR20020026366A (ko) * 1999-08-09 2002-04-09 한스 루돌프 하우스, 헨리에데 부르너 단백질의 생산

Also Published As

Publication number Publication date
IL147411A (en) 2013-12-31
CZ303341B6 (cs) 2012-08-08
JP5480458B2 (ja) 2014-04-23
JP2003506092A (ja) 2003-02-18
HU230117B1 (hu) 2015-08-28
HUP0203051A2 (hu) 2002-12-28
AU6699800A (en) 2001-03-05
NO20020507L (no) 2002-04-05
EP1200603B1 (en) 2014-12-24
MXPA02001425A (es) 2002-08-12
CN1230542C (zh) 2005-12-07
EP1200603A1 (en) 2002-05-02
US20040215008A1 (en) 2004-10-28
AU775681B2 (en) 2004-08-12
CA2380302A1 (en) 2001-02-15
NO20020507D0 (no) 2002-01-31
CN1367837A (zh) 2002-09-04
WO2001011056A1 (en) 2001-02-15
HUP0203051A3 (en) 2003-12-29
KR100735791B1 (ko) 2007-07-06
TR200200048T2 (tr) 2002-04-22
PL203708B1 (pl) 2009-11-30
SK1872002A3 (en) 2002-07-02
PL353090A1 (en) 2003-10-06
KR20020021175A (ko) 2002-03-18
SK288290B6 (sk) 2015-07-01
CA2380302C (en) 2013-02-26
US7378512B2 (en) 2008-05-27
IL147411A0 (en) 2002-08-14
HK1047127B (en) 2015-08-21
CZ2002480A3 (cs) 2002-05-15
HK1047127A1 (en) 2003-02-07

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NO329246B1 (no) Fremgangsmate for fremstilling av proteiner ved autoproteolytisk spalting ved bruk av ekspresjonsvektor og bakterievertscelle
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