SE0702354L - Ways to detect previous ethanol intake - Google Patents

Ways to detect previous ethanol intake

Info

Publication number
SE0702354L
SE0702354L SE0702354A SE0702354A SE0702354L SE 0702354 L SE0702354 L SE 0702354L SE 0702354 A SE0702354 A SE 0702354A SE 0702354 A SE0702354 A SE 0702354A SE 0702354 L SE0702354 L SE 0702354L
Authority
SE
Sweden
Prior art keywords
ethanol
peth
intake
quantification
ways
Prior art date
Application number
SE0702354A
Other languages
Swedish (sv)
Other versions
SE531588C2 (en
Inventor
Arthur Varga
Staffan Nilsson
Original Assignee
Anaxcess Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anaxcess Ab filed Critical Anaxcess Ab
Priority to SE0702354A priority Critical patent/SE531588C2/en
Priority to PCT/SE2008/051187 priority patent/WO2009054784A1/en
Publication of SE0702354L publication Critical patent/SE0702354L/en
Publication of SE531588C2 publication Critical patent/SE531588C2/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/98—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving alcohol, e.g. ethanol in breath
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2405/00—Assays, e.g. immunoassays or enzyme assays, involving lipids
    • G01N2405/04—Phospholipids, i.e. phosphoglycerides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Endocrinology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention comprises a method for assessing previous intake of ethanol, the main alcohol constituent in alcoholic beverages, from a biological sample. Level of ethanol intake is determined by measuring phosphatidylethanol (Peth), which is formed in cell membranes via the enzyme phospholipase D, only in the presence of ethanol. The present invention mainly relates to an analytical method based on capillary electrophoresis in which Peth is separated from other compounds, enabling detection and quantification. The invention further relates to an analytical method in which non aqueous capillary electrophoresis and stacking is combined, in order to enhance detection and quantification of Peth. The present invention also relates to a method for detecting and/or quantifying ethanol intake by determining a ratio of at least two ethanol metabolites, preferably Peth and Ethyl phosphate.
SE0702354A 2007-10-23 2007-10-23 Ways to detect previous ethanol intake SE531588C2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
SE0702354A SE531588C2 (en) 2007-10-23 2007-10-23 Ways to detect previous ethanol intake
PCT/SE2008/051187 WO2009054784A1 (en) 2007-10-23 2008-10-22 Method for assessing previous ethanol intake

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE0702354A SE531588C2 (en) 2007-10-23 2007-10-23 Ways to detect previous ethanol intake

Publications (2)

Publication Number Publication Date
SE0702354L true SE0702354L (en) 2009-04-23
SE531588C2 SE531588C2 (en) 2009-06-02

Family

ID=40579769

Family Applications (1)

Application Number Title Priority Date Filing Date
SE0702354A SE531588C2 (en) 2007-10-23 2007-10-23 Ways to detect previous ethanol intake

Country Status (2)

Country Link
SE (1) SE531588C2 (en)
WO (1) WO2009054784A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8795980B2 (en) 2010-07-09 2014-08-05 Pethmark AB Methods for determination of previous ethanol consumption
US20230228654A1 (en) 2020-07-02 2023-07-20 Capitainer Ab Blood Sampling Device and Method for PEth Measurement
SE2050826A1 (en) 2020-07-02 2022-01-03 Capitainer Ab Functionalized blood sampling device and method for peth measurement
CN113970603A (en) * 2020-07-24 2022-01-25 复旦大学 Biomarkers used to differentiate drinking deaths from postmortem drinking
CN119688807B (en) * 2024-12-11 2025-12-26 中国科学院大连化学物理研究所 A method for determining the content of HAN and hydroxyethylhydrazine nitrate in hydroxylamine nitrate propellants.

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060084134A1 (en) * 2004-06-10 2006-04-20 Wurst Friedrich M Direct ethanol metabolite ethyl sulfate as an useful diagnostic and therapeutic marker of alcohol consumption

Also Published As

Publication number Publication date
WO2009054784A1 (en) 2009-04-30
SE531588C2 (en) 2009-06-02

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Legal Events

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