SU1490961A1 - Способ получения обратной транскриптазы вируса саркомы рауса - Google Patents
Способ получения обратной транскриптазы вируса саркомы раусаInfo
- Publication number
- SU1490961A1 SU1490961A1 SU4262311/13A SU4262311A SU1490961A1 SU 1490961 A1 SU1490961 A1 SU 1490961A1 SU 4262311/13 A SU4262311/13 A SU 4262311/13A SU 4262311 A SU4262311 A SU 4262311A SU 1490961 A1 SU1490961 A1 SU 1490961A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- sarcoma virus
- rous sarcoma
- gene
- enzyme
- pol
- Prior art date
Links
- 241000714474 Rous sarcoma virus Species 0.000 title abstract 3
- 102100034343 Integrase Human genes 0.000 title abstract 2
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 3
- 108090000790 Enzymes Proteins 0.000 abstract 3
- 108700004029 pol Genes Proteins 0.000 abstract 3
- 101150088264 pol gene Proteins 0.000 abstract 3
- 108020004414 DNA Proteins 0.000 abstract 2
- 241000588724 Escherichia coli Species 0.000 abstract 2
- 108020004511 Recombinant DNA Proteins 0.000 abstract 2
- 230000009466 transformation Effects 0.000 abstract 2
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 238000010353 genetic engineering Methods 0.000 abstract 1
- 230000006698 induction Effects 0.000 abstract 1
- 230000000977 initiatory effect Effects 0.000 abstract 1
- 238000004255 ion exchange chromatography Methods 0.000 abstract 1
- 108020004999 messenger RNA Proteins 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 230000003612 virological effect Effects 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Изобретение относится к генетической инженерии. Согласно предлагаемому способу кодирующий обратную транскриптазу pol-ген ДНК вируса саркомы Рауса встраивают в плазмидный вектор экспрессии, в частности в pUC 9, непосредственно за lac-промотором, полученные фрагменты используют для трансформации бактерий E.coli, после чего отбирают рекомбинантную ДНК, обладающую набором последовательностей, обеспечивающих репрессию и индукцию вирусного pol-гена, связывание его МРНК с рибосомой, а также последовательностью инициации и терминации трансляции. Полученной рекомбинантной ДНК трансформируют E.coli, клетки бактерий разрушают и фермент очищают ионообменной хроматографией.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU4262311/13A SU1490961A1 (ru) | 1987-06-17 | 1987-06-17 | Способ получения обратной транскриптазы вируса саркомы рауса |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU4262311/13A SU1490961A1 (ru) | 1987-06-17 | 1987-06-17 | Способ получения обратной транскриптазы вируса саркомы рауса |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU1490961A1 true SU1490961A1 (ru) | 1994-07-15 |
Family
ID=60526595
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU4262311/13A SU1490961A1 (ru) | 1987-06-17 | 1987-06-17 | Способ получения обратной транскриптазы вируса саркомы рауса |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU1490961A1 (ru) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6518019B2 (en) | 1997-04-22 | 2003-02-11 | Invitrogen Corporation | Compositions and methods for reverse transcription of nucleic acid molecules |
-
1987
- 1987-06-17 SU SU4262311/13A patent/SU1490961A1/ru active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6518019B2 (en) | 1997-04-22 | 2003-02-11 | Invitrogen Corporation | Compositions and methods for reverse transcription of nucleic acid molecules |
| US6835561B1 (en) | 1997-04-22 | 2004-12-28 | Invitrogen Corporation | Composition of reverse transcriptases and mutants thereof |
| US6989259B2 (en) | 1997-04-22 | 2006-01-24 | Invitrogen Corporation | Recombinant methods for making reverse transcriptases and mutants thereof |
| US7115406B2 (en) | 1997-04-22 | 2006-10-03 | Invitrogen Corporation | Recombinant method for making RSV reverse transcriptases and mutants thereof |
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