WO1988009981A3 - Polymers, and their use as gels for electrophoresis - Google Patents

Polymers, and their use as gels for electrophoresis Download PDF

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Publication number
WO1988009981A3
WO1988009981A3 PCT/EP1988/000515 EP8800515W WO8809981A3 WO 1988009981 A3 WO1988009981 A3 WO 1988009981A3 EP 8800515 W EP8800515 W EP 8800515W WO 8809981 A3 WO8809981 A3 WO 8809981A3
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WO
WIPO (PCT)
Prior art keywords
gels
nat
poly
pct
bis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP1988/000515
Other languages
French (fr)
Other versions
WO1988009981A2 (en
Inventor
Branko Kozulic
Klaus Mosbach
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to US07/328,123 priority Critical patent/US5319046A/en
Priority to AT88905199T priority patent/ATE89287T1/en
Publication of WO1988009981A2 publication Critical patent/WO1988009981A2/en
Publication of WO1988009981A3 publication Critical patent/WO1988009981A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/52—Amides or imides
    • C08F220/54—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide
    • C08F220/58—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide containing oxygen in addition to the carbonamido oxygen, e.g. N-methylolacrylamide, N-(meth)acryloylmorpholine
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S524/00—Synthetic resins or natural rubbers -- part of the class 520 series
    • Y10S524/916—Hydrogel compositions

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Macromonomer-Based Addition Polymer (AREA)
  • Peptides Or Proteins (AREA)
  • Graft Or Block Polymers (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

PCT No. PCT/EP88/00515 Sec. 371 Date Jan. 31, 1989 Sec. 102(e) Date Jan. 31, 1989 PCT Filed Jun. 10, 1988 PCT Pub. No. WO88/09981 PCT Pub. Date Dec. 15, 1988.A novel synthesis of N-acryloyl-tris-(hydroxymethyl)aminomethane (NAT) is described. The polymerization kinetics and transparency of the poly(NAT) gels crosslinked by a crosslinker (CL) e.g. N,N'-methylenebisacrylamide (Bis), i.e. poly(NAT+Bis) polymer, are shown. Poly(NAT+Bis) gradient (4-24%) gels were prepared and found to resolve native proteins according to their size. The exclusion limit of these gels is over 3x106 Da. This is more than threefold higher than the exclusion limit of the polyacrylamide gradient gel of the same concentration. Therefore poly(NAT+CL) gels are better than polyacrylamide gels for the resolution of large proteins. It was demonstrated that poly(NAT+CL)-polyacrylamide composite gels could be prepared. The poly(NAT+CL) gels were found to be advantageous also for isoelectric focussing in carrier ampholytes or immobilized pH gradients. Poly(NAT+CL) gels are also very good for separation of nucleic acids. The resolution is particularly good in the range between several hundred to 4-5000 base pairs. Under the optimized conditions, DNA molecules of more than 20 kbases can be perfectly resolved.
PCT/EP1988/000515 1987-06-13 1988-06-10 Polymers, and their use as gels for electrophoresis Ceased WO1988009981A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US07/328,123 US5319046A (en) 1987-06-13 1988-06-10 Polymers, and their use as gels for electrophoresis
AT88905199T ATE89287T1 (en) 1987-06-13 1988-06-10 POLYMERS AND THEIR USE IN ELECTROPHORESIS.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB8713854 1987-06-13
GB878713854A GB8713854D0 (en) 1987-06-13 1987-06-13 Poly-n-acryloyl-tris(hydroxymethyl)aminomethane gels

Publications (2)

Publication Number Publication Date
WO1988009981A2 WO1988009981A2 (en) 1988-12-15
WO1988009981A3 true WO1988009981A3 (en) 1989-02-09

Family

ID=10618866

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1988/000515 Ceased WO1988009981A2 (en) 1987-06-13 1988-06-10 Polymers, and their use as gels for electrophoresis

Country Status (7)

Country Link
US (1) US5319046A (en)
EP (1) EP0318551B1 (en)
JP (1) JP2654681B2 (en)
AT (1) ATE89287T1 (en)
DE (1) DE3880978T2 (en)
GB (2) GB8713854D0 (en)
WO (1) WO1988009981A2 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5238545A (en) * 1991-02-27 1993-08-24 W. R. Grace & Co.-Conn. Electrophoretic gel for separation and recovery of substances and its use
US5225062A (en) * 1991-02-27 1993-07-06 W. R. Grace & Co. -Conn. Electrophoretic gel for separation and recovery of substances and its use
US5631337A (en) * 1996-01-19 1997-05-20 Soane Bioscience Thermoreversible hydrogels comprising linear copolymers and their use in electrophoresis
US5883211A (en) * 1996-01-19 1999-03-16 Aclara Biosciences, Inc. Thermoreversible hydrogels comprising linear copolymers and their use in electrophoresis
US5840877A (en) * 1996-09-05 1998-11-24 Guest Elchrom Scientific Electrophoresis gels of enhanced selectivity
US6884842B2 (en) 1997-10-14 2005-04-26 Alnis Biosciences, Inc. Molecular compounds having complementary surfaces to targets
US6117293A (en) * 1998-07-31 2000-09-12 Biowhittaker Molecular Applications, Inc. Method for producing hydrophilic monomers and uses thereof
US6464850B1 (en) 1998-07-31 2002-10-15 Biowhittaker Molecular Applications, Inc. Method for producing hydrophilic monomers and uses thereof
SE517422C2 (en) * 2000-10-06 2002-06-04 Bioglan Ab Production of starch for parenteral administration in form of microparticles, comprises washing starch, dissolving in aqueous medium, and subjecting to molecular weight reduction by shearing
US7479521B2 (en) 2001-10-17 2009-01-20 Societe D'exploitation De Produits Pour Les Industries Chimiques (Seppic) Tris(hydroxymethyl) acrylamidomethane polymer, inverse latex and microlatex containing same, use of said polymer, inverse latex and microlatex
FR2830867B1 (en) * 2001-10-17 2006-12-22 Seppic Sa POLYMER OF TRIS (HYDROXYMETHYL) ACRYLAMIDOMETHANE, REVERSE LATEX AND MICROLATEX BY CONTAINING THE SAME, USE OF SAID POLYMER, REVERSE LATEX AND REVERSE MICROLATEX
US7326324B2 (en) * 2002-11-20 2008-02-05 Shin-Etsu Chemical Co., Ltd. Purification and use of gellan in electrophoresis gels
US7022214B2 (en) * 2004-01-21 2006-04-04 Bio-Rad Laboratories, Inc. Carrier ampholytes of high pH range
US7407816B2 (en) * 2004-05-07 2008-08-05 Gentius, Inc Isoelectric particles and uses thereof
EP2143740B1 (en) † 2004-08-19 2013-06-19 Covidien LP Water-swellable copolymers and articles and coating made therefrom
US10933168B2 (en) 2015-12-04 2021-03-02 Poly-Med, Inc. Double network hydrogel with anionic polymer and uses therof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US419784A (en) * 1890-01-21 William stoodard mead
DE2159501A1 (en) * 1970-12-09 1972-06-22 Commercial Solvents Corp., Terre Haute, Ind. (V.StA.) Bis or tris-hydroxymethylamides of (meth)acrylic - acid prodn - by reacting corresp alkanolamine with (meth)acrylic acid
EP0040124A1 (en) * 1980-05-09 1981-11-18 Pharmuka Laboratoires Hydrophilic copolymers based on N-tris(hydroxy-methyl)methylacryl amide, process for their preparation, aqueous gel of these copolymers and their use as ion exchangers

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2359873A1 (en) * 1976-07-27 1978-02-24 Sebia Sa Crosslinked n-methylol-acrylamide gels contg. polysaccharide - on polyester film support, for electrophoretic sepn. of lipoprotein(s)
FR2378808A1 (en) * 1977-01-28 1978-08-25 Mar Pha Etu Expl Marques NEW HYDROPHILIC COPOLYMERS BASED ON N- (TRIS (HYDROXY-METHYL) METHYL) ACRYLAMIDE OR N- (TRIS (HYDROXYMETHYL) METHYL) METHACRYLAMIDE, THEIR PREPARATION AND THEIR USE IN SEPARATION TECHNIQUES
US4806434A (en) * 1984-07-06 1989-02-21 Fuji Photo Film Co., Ltd. Medium for electrophoresis

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US419784A (en) * 1890-01-21 William stoodard mead
DE2159501A1 (en) * 1970-12-09 1972-06-22 Commercial Solvents Corp., Terre Haute, Ind. (V.StA.) Bis or tris-hydroxymethylamides of (meth)acrylic - acid prodn - by reacting corresp alkanolamine with (meth)acrylic acid
EP0040124A1 (en) * 1980-05-09 1981-11-18 Pharmuka Laboratoires Hydrophilic copolymers based on N-tris(hydroxy-methyl)methylacryl amide, process for their preparation, aqueous gel of these copolymers and their use as ion exchangers

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Analytical Biochemistry, volume 163, 1987, Academic Press, Inc., M. Kozulic et al.: "Poly-N-acryloyl-tris gels as anticonvection media for electrophoresis and isoelectric focusing", pages 506-512 *

Also Published As

Publication number Publication date
GB8813744D0 (en) 1988-07-13
JP2654681B2 (en) 1997-09-17
EP0318551B1 (en) 1993-05-12
EP0318551A1 (en) 1989-06-07
GB2206594A (en) 1989-01-11
ATE89287T1 (en) 1993-05-15
JPH01503629A (en) 1989-12-07
GB8713854D0 (en) 1987-07-15
WO1988009981A2 (en) 1988-12-15
DE3880978T2 (en) 1993-10-14
DE3880978D1 (en) 1993-06-17
US5319046A (en) 1994-06-07

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