WO1990006044A3 - Process for producing cdna - Google Patents

Process for producing cdna Download PDF

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Publication number
WO1990006044A3
WO1990006044A3 PCT/EP1989/001418 EP8901418W WO9006044A3 WO 1990006044 A3 WO1990006044 A3 WO 1990006044A3 EP 8901418 W EP8901418 W EP 8901418W WO 9006044 A3 WO9006044 A3 WO 9006044A3
Authority
WO
WIPO (PCT)
Prior art keywords
mrna
support
dna
insoluble support
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP1989/001418
Other languages
French (fr)
Other versions
WO1990006044A2 (en
Inventor
Erik Hornes
Lars Korsnes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies AS
Original Assignee
Dynal AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB888827158A external-priority patent/GB8827158D0/en
Priority claimed from GB888827159A external-priority patent/GB8827159D0/en
Application filed by Dynal AS filed Critical Dynal AS
Priority to DE68922743T priority Critical patent/DE68922743T2/en
Priority to EP90900183A priority patent/EP0444119B1/en
Publication of WO1990006044A2 publication Critical patent/WO1990006044A2/en
Publication of WO1990006044A3 publication Critical patent/WO1990006044A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Luminescent Compositions (AREA)
  • Saccharide Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

The process includes the steps of: a) contacting a liquid containing mRNA with an insoluble support having DNA probes attached thereto via the 5'-terminus thereof whereby the mRNA is hybridised to said probes and hence to said support; b) removing said liquid; and c) adding enzymes and nucleotides in solution whereby the probe functions as a primer to produce single stranded cDNA on the mRNA templates. The DNA probe is oligo-dT or a specific DNA sequence which may correspond to a conserved region in a class of mRNA molecules. The insoluble support may consist of magnetic particles which are monodisperse and/or superparamagnetic.
PCT/EP1989/001418 1988-11-21 1989-11-21 PROCESS FOR PRODUCING cDNA Ceased WO1990006044A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE68922743T DE68922743T2 (en) 1988-11-21 1989-11-21 METHOD FOR PRODUCING CDNS.
EP90900183A EP0444119B1 (en) 1988-11-21 1989-11-21 PROCESS FOR PRODUCING cDNA

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB888827158A GB8827158D0 (en) 1988-11-21 1988-11-21 Process for producing cdna
GB888827159A GB8827159D0 (en) 1988-11-21 1988-11-21 Subtraction cdna library construction
GB8827159.8 1988-11-21
GB8827158.0 1988-11-21

Publications (2)

Publication Number Publication Date
WO1990006044A2 WO1990006044A2 (en) 1990-06-14
WO1990006044A3 true WO1990006044A3 (en) 1990-11-01

Family

ID=26294647

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1989/001418 Ceased WO1990006044A2 (en) 1988-11-21 1989-11-21 PROCESS FOR PRODUCING cDNA

Country Status (6)

Country Link
EP (1) EP0444119B1 (en)
JP (1) JP2960776B2 (en)
AT (1) ATE122721T1 (en)
CA (1) CA2003500C (en)
DE (1) DE68922743T2 (en)
WO (1) WO1990006044A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7329496B2 (en) 1990-12-06 2008-02-12 Affymetrix, Inc. Sequencing of surface immobilized polymers utilizing microflourescence detection

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0533952A4 (en) * 1991-04-15 1993-07-07 Sumitomo Electric Industries, Ltd. Process for separating or extracting nucleic acid from specimen containing the same
US6316182B1 (en) * 1992-01-15 2001-11-13 Nen Life Science Products, Inc. Detection of reverse transcriptase by DNA hybridization assay
US6300058B1 (en) 1992-01-29 2001-10-09 Hitachi Chemical Research Center, Inc. Method for measuring messenger RNA
CA2128891A1 (en) * 1992-01-29 1993-08-05 Cylia Keller Polynucleotide immobilized support
WO1994002636A1 (en) * 1992-07-28 1994-02-03 Hitachi Chemical Company Ltd. Gene detection system
US5580971A (en) * 1992-07-28 1996-12-03 Hitachi Chemical Company, Ltd. Fungal detection system based on rRNA probes
US5591841A (en) * 1993-01-14 1997-01-07 Ji; Huamin Rapid purification of circular DNA by triplex-mediated affinity capture
US5610287A (en) * 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
WO1997031105A1 (en) * 1996-02-25 1997-08-28 Precision System Science Co., Ltd. Method for treating biopolymers, microorganisms or materials by using more than one type of magnetic particles
US5712127A (en) * 1996-04-29 1998-01-27 Genescape Inc. Subtractive amplification
WO1998051699A1 (en) * 1997-05-12 1998-11-19 Life Technologies, Inc. Methods for production and purification of nucleic acid molecules
US6399334B1 (en) 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof
US6083727A (en) * 1999-06-30 2000-07-04 Incyte Pharmaceuticals Inc. Methods and compositions for producing 5' enriched cDNA libraries
ES2264440T3 (en) 2000-03-24 2007-01-01 Micromet Ag MRNA AMPLIFICATION
JP4735926B2 (en) * 2004-05-27 2011-07-27 アイシン精機株式会社 Highly efficient recovery method of nucleic acid
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
CN113278606A (en) * 2020-02-03 2021-08-20 帝肯基因组学公司 Bead reuse in sample cleaning
GB2621159B (en) * 2022-08-04 2025-08-13 Wobble Genomics Ltd Methods of preparing processed nucleic acid samples and detecting nucleic acids and devices therefor

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1985004674A1 (en) * 1984-04-05 1985-10-24 Life Technologies, Inc. Immobilization of nucleic acids
EP0184056A2 (en) * 1984-11-27 1986-06-11 Molecular Diagnostics, Inc. Large scale production of DNA probes
EP0223618A2 (en) * 1985-07-18 1987-05-27 New York Medical College Automatable process for nucleotide sequencing
EP0265244A2 (en) * 1986-10-23 1988-04-27 Amoco Corporation Target and background capture methods and apparatus for affinity assays
WO1988007585A1 (en) * 1987-04-02 1988-10-06 Genelabs, Incorporated Equal-abundance transcript composition and method
EP0288737A1 (en) * 1987-04-01 1988-11-02 Miles Inc. Rapid hybridization assay using latex-immobilized probe

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1985004674A1 (en) * 1984-04-05 1985-10-24 Life Technologies, Inc. Immobilization of nucleic acids
EP0184056A2 (en) * 1984-11-27 1986-06-11 Molecular Diagnostics, Inc. Large scale production of DNA probes
EP0223618A2 (en) * 1985-07-18 1987-05-27 New York Medical College Automatable process for nucleotide sequencing
EP0265244A2 (en) * 1986-10-23 1988-04-27 Amoco Corporation Target and background capture methods and apparatus for affinity assays
EP0288737A1 (en) * 1987-04-01 1988-11-02 Miles Inc. Rapid hybridization assay using latex-immobilized probe
WO1988007585A1 (en) * 1987-04-02 1988-10-06 Genelabs, Incorporated Equal-abundance transcript composition and method

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Cell, Volume 21, October 1980, MIT, C.R. ZIMMERMAN et al.: "Molecular Cloning and Selection of Genes Regulated in Aspergillus Development", pages 709-715 see the whole article *
Nature, Volume 308, 8 March 1984, S.M. HEDRICK et al.: "Isolation of cDNA Clones Encoding T Cell-Specific Membrane-Associated Proteins", pages 149-153 see the whole article *
Nucleic Acids Research, Volume 15, No. 13, 10 July 1987, IRL Press, (Oxford, GB), T.R. GINGERAS et al.: "Hybridization Properties of Immobilized Nucleic Acids", pages 5373-5389 see "Introduction; "Materials and Methods" *
Nucleic Acids Research, Volume 16, No. 13, 11 July 1988, IRL Press, (Oxford, GB), T. ATKINSON et al.: "A Conventient Procedure for the Synthesis of Oligodeoxyribonucleotide Affinity columns for the Isolation of mRNA", page 6232 see the whole article *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7329496B2 (en) 1990-12-06 2008-02-12 Affymetrix, Inc. Sequencing of surface immobilized polymers utilizing microflourescence detection
US7459275B2 (en) 1990-12-06 2008-12-02 Affymetrix, Inc. Sequencing of surface immobilized polymers utilizing microfluorescence detection

Also Published As

Publication number Publication date
CA2003500A1 (en) 1990-05-21
EP0444119A1 (en) 1991-09-04
WO1990006044A2 (en) 1990-06-14
JP2960776B2 (en) 1999-10-12
JPH04501958A (en) 1992-04-09
AU4758590A (en) 1990-06-26
AU627815B2 (en) 1992-09-03
DE68922743D1 (en) 1995-06-22
ATE122721T1 (en) 1995-06-15
CA2003500C (en) 2000-01-18
EP0444119B1 (en) 1995-05-17
DE68922743T2 (en) 1995-10-26

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