WO1991002071A3 - Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof - Google Patents

Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof Download PDF

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Publication number
WO1991002071A3
WO1991002071A3 PCT/US1990/004462 US9004462W WO9102071A3 WO 1991002071 A3 WO1991002071 A3 WO 1991002071A3 US 9004462 W US9004462 W US 9004462W WO 9102071 A3 WO9102071 A3 WO 9102071A3
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WO
WIPO (PCT)
Prior art keywords
cells
methods
transformed
production
compositions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1990/004462
Other languages
French (fr)
Other versions
WO1991002071A2 (en
Inventor
Thomas R Adams
Whitney R Adams Jr
Sheryl A Chambers
Richard J Daines
William J Gordon-Kamm
Albert P Kausch
Roger W Krueger
Peggy G Lemaux
Catherine J Mackey
Mary L Mangano
James V O'brien
Thomas B Rice
T Michael Spencer
William G Start
Nancy Willetts
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DeKalb Genetics Corp
Original Assignee
DeKalb Plant Genetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27013786&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1991002071(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by DeKalb Plant Genetics Inc filed Critical DeKalb Plant Genetics Inc
Priority to DE69031711T priority Critical patent/DE69031711T2/en
Priority to BR909007588A priority patent/BR9007588A/en
Priority to HU9200394A priority patent/HU218465B/en
Priority to EP90912722A priority patent/EP0485506B1/en
Priority to CA002064761A priority patent/CA2064761C/en
Publication of WO1991002071A2 publication Critical patent/WO1991002071A2/en
Anticipated expiration legal-status Critical
Publication of WO1991002071A3 publication Critical patent/WO1991002071A3/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0025—Culture media for plant cell or plant tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Environmental Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fertilizers (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Seasonings (AREA)

Abstract

This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
PCT/US1990/004462 1989-08-09 1990-08-08 Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof Ceased WO1991002071A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
DE69031711T DE69031711T2 (en) 1989-08-09 1990-08-08 METHODS AND COMPOSITIONS FOR THE PRODUCTION OF STABLY TRANSFORMED, FERTILIZABLE CORN PLANTS AND CELLS THEREFOR
BR909007588A BR9007588A (en) 1989-08-09 1990-08-08 PROCESS AND COMPOSITIONS FOR THE PRODUCTION OF FERTILE MONOCOTILLEDONE PLANTS, STABLELY TRANSFORMED, AND THEIR CELLS
HU9200394A HU218465B (en) 1989-08-09 1990-08-08 Methods and Compositions for Stably Transforming, Producing Monocotyledonous Plants and Their Cells
EP90912722A EP0485506B1 (en) 1989-08-09 1990-08-08 Methods and compositions for the production of stably transformed, fertile maize plants and cells thereof
CA002064761A CA2064761C (en) 1989-08-09 1990-08-08 Methods and compositions for the production of stably transformed fertile monocot plants and cells thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US39217689A 1989-08-09 1989-08-09
US392,176 1989-08-09
US51329890A 1990-04-17 1990-04-17
US513,298 1990-04-17

Publications (2)

Publication Number Publication Date
WO1991002071A2 WO1991002071A2 (en) 1991-02-21
WO1991002071A3 true WO1991002071A3 (en) 1992-05-14

Family

ID=27013786

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1990/004462 Ceased WO1991002071A2 (en) 1989-08-09 1990-08-08 Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof

Country Status (9)

Country Link
EP (2) EP0485506B1 (en)
JP (1) JPH05501352A (en)
AT (1) ATE160174T1 (en)
AU (1) AU644097B2 (en)
BR (1) BR9007588A (en)
DE (1) DE69031711T2 (en)
ES (1) ES2110417T3 (en)
HU (1) HU218465B (en)
WO (1) WO1991002071A2 (en)

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US6960709B1 (en) 1993-08-25 2005-11-01 Dekalb Genetics Corporation Method for altering the nutritional content of plant seed
US7064248B2 (en) 1990-01-22 2006-06-20 Dekalb Genetics Corp. Method of preparing fertile transgenic corn plants by microprojectile bombardment
US7615685B2 (en) 1990-01-22 2009-11-10 Dekalb Genetics Corporation Methods of producing human or animal food from stably transformed, fertile maize plants
US8946460B2 (en) 2012-06-15 2015-02-03 Commonwealth Scientific And Industrial Research Organisation Process for producing polyunsaturated fatty acids in an esterified form
US12709759B2 (en) 2019-11-11 2026-08-18 Commonwealth Scientific And Industrial Research Organisation Puccinia resistance gene

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HU218465B (en) 2000-09-28
DE69031711D1 (en) 1997-12-18
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AU6290390A (en) 1991-03-11
ES2110417T3 (en) 1998-02-16

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