WO1991013075A3 - Method and reagent for determining specific nucleotide variations - Google Patents

Method and reagent for determining specific nucleotide variations Download PDF

Info

Publication number
WO1991013075A3
WO1991013075A3 PCT/FI1991/000046 FI9100046W WO9113075A3 WO 1991013075 A3 WO1991013075 A3 WO 1991013075A3 FI 9100046 W FI9100046 W FI 9100046W WO 9113075 A3 WO9113075 A3 WO 9113075A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection step
incorporation
variable nucleotide
reagent
primer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FI1991/000046
Other languages
French (fr)
Other versions
WO1991013075A2 (en
Inventor
Hans Soederlund
Ann-Christine Syvaenen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Orion Oyj
Original Assignee
Orion Yhtyma Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to DE69131233T priority Critical patent/DE69131233T2/en
Priority to DE0648280T priority patent/DE648280T1/en
Priority to CA002071537A priority patent/CA2071537C/en
Priority to DK91903942T priority patent/DK0648280T3/en
Priority to EP91903942A priority patent/EP0648280B1/en
Application filed by Orion Yhtyma Oy filed Critical Orion Yhtyma Oy
Publication of WO1991013075A2 publication Critical patent/WO1991013075A2/en
Publication of WO1991013075A3 publication Critical patent/WO1991013075A3/en
Priority to NO19923116A priority patent/NO311529B1/en
Priority to FI923653A priority patent/FI102297B/en
Anticipated expiration legal-status Critical
Priority to GR950300047T priority patent/GR950300047T1/en
Priority to GR990401879T priority patent/GR3030790T3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • AIDS & HIV (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)

Abstract

Detection of variable nucleotide(s) is based on primer extension and incorporation of detectable nucleoside triphosphates. By selecting the detection step primers from the region immediately adjacent to the variable nucleotide, this variation can be detected after incorporation of as few as one nucleoside triphosphate. Labelled nucleoside triphosphates matching the variable nucleotide are added and the incorporation of a label into the detection step primer is measured. The selection of the detection step primer is important to the method according to this invention and is dependent on the nucleotide sequence of interest. The detection step primers are preferably selected so to span the region immediately toward the 3' end from the variable nucleotide to be detected. The method is useful in identifying specific point mutations and genetic variations.
PCT/FI1991/000046 1990-02-16 1991-02-15 Method and reagent for determining specific nucleotide variations Ceased WO1991013075A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
DE69131233T DE69131233T2 (en) 1990-02-16 1991-02-15 METHOD AND REAGENT FOR DETECTING SPECIFIC NUCLEOTIDE VARIATIONS
DE0648280T DE648280T1 (en) 1990-02-16 1991-02-15 METHOD AND REAGENT FOR DETECTING SPECIFIC NUCLEOTIDE VARIATIONS.
CA002071537A CA2071537C (en) 1990-02-16 1991-02-15 Method and reagent for determining specific nucleotide variations
DK91903942T DK0648280T3 (en) 1990-02-16 1991-02-15 Methods and Reagents for Determining Specific Nucleotide Variations
EP91903942A EP0648280B1 (en) 1990-02-16 1991-02-15 Method and reagent for determining specific nucleotide variations
NO19923116A NO311529B1 (en) 1990-02-16 1992-08-10 Method and Reagent for Determining Specific Nucleotide Variations
FI923653A FI102297B (en) 1990-02-16 1992-08-14 Method and Reagents for Determining Specific Nucleotide Variations
GR950300047T GR950300047T1 (en) 1990-02-16 1995-07-31 Method and reagent for determining specific nucleotide variations.
GR990401879T GR3030790T3 (en) 1990-02-16 1999-07-14 Method and reagent for determining specific nucleotide variations.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48200590A 1990-02-16 1990-02-16
US482,005 1990-02-16

Publications (2)

Publication Number Publication Date
WO1991013075A2 WO1991013075A2 (en) 1991-09-05
WO1991013075A3 true WO1991013075A3 (en) 1991-10-17

Family

ID=23914258

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FI1991/000046 Ceased WO1991013075A2 (en) 1990-02-16 1991-02-15 Method and reagent for determining specific nucleotide variations

Country Status (18)

Country Link
EP (1) EP0648280B1 (en)
JP (1) JP2786011B2 (en)
AT (1) ATE180019T1 (en)
AU (1) AU642709B2 (en)
CA (1) CA2071537C (en)
DE (2) DE648280T1 (en)
DK (1) DK0648280T3 (en)
ES (1) ES2072235T3 (en)
FI (1) FI102297B (en)
GR (2) GR950300047T1 (en)
HU (1) HU211058B (en)
IE (1) IE910525A1 (en)
IL (1) IL97222A (en)
NO (1) NO311529B1 (en)
NZ (1) NZ237134A (en)
PT (1) PT96776B (en)
WO (1) WO1991013075A2 (en)
ZA (1) ZA911152B (en)

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6949633B1 (en) 1995-05-22 2005-09-27 Sequenom, Inc. Primers useful for sizing nucleic acids
US6982431B2 (en) 1998-08-31 2006-01-03 Molecular Devices Corporation Sample analysis systems
US7056669B2 (en) 1996-11-05 2006-06-06 Clinical Micro Sensors, Inc. AC methods for the detection of nucleic acids
US7070921B2 (en) 2000-04-28 2006-07-04 Molecular Devices Corporation Molecular modification assays
US7074563B2 (en) 1995-03-17 2006-07-11 Sequenom, Inc. Mass spectrometric methods for detecting mutations in a target nucleic acid
US7125668B2 (en) 1996-11-05 2006-10-24 Clinical Micro Sensors, Inc. Electrodes linked via conductive oligomers to nucleic acids
US7198893B1 (en) 1996-11-06 2007-04-03 Sequenom, Inc. DNA diagnostics based on mass spectrometry
US7329496B2 (en) 1990-12-06 2008-02-12 Affymetrix, Inc. Sequencing of surface immobilized polymers utilizing microflourescence detection
US7384749B2 (en) 1996-11-05 2008-06-10 Clinical Micro Sensors, Inc. Electrodes linked via conductive oligomers to nucleic acids
US7432342B2 (en) 2002-05-03 2008-10-07 Sequenom, Inc. Kinase anchor protein muteins, peptides thereof and related documents
US7632651B2 (en) 1997-09-15 2009-12-15 Mds Analytical Technologies (Us) Inc. Molecular modification assays
US7851146B2 (en) 1993-12-10 2010-12-14 California Institute Of Technology Nucleic acid mediated electron transfer
USRE44693E1 (en) 1996-11-06 2014-01-07 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
US9243284B2 (en) 2000-12-01 2016-01-26 Life Technologies Corporation Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis
US9279148B2 (en) 1999-04-20 2016-03-08 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US9588051B2 (en) 2004-09-17 2017-03-07 Pacific Biosciences Of California, Inc. Apparatus and method for performing nucleic acid analysis

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6013431A (en) 1990-02-16 2000-01-11 Molecular Tool, Inc. Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators
US6004744A (en) 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
WO1992016180A2 (en) * 1991-03-13 1992-10-01 Siska Diagnostics, Inc. Detection of 3'-azido-3'-deoxythymidine resistance
US7026115B1 (en) 1991-09-24 2006-04-11 Keygene N.V. Selective restriction fragment amplification: fingerprinting
US20100267023A1 (en) 1992-09-24 2010-10-21 Keygene N.V. Selective restriction fragment amplification: fingerprinting
GB9208000D0 (en) * 1992-04-10 1992-05-27 Univ London Quantitative viral assay
US5750335A (en) * 1992-04-24 1998-05-12 Massachusetts Institute Of Technology Screening for genetic variation
ATE198358T1 (en) * 1992-04-27 2001-01-15 Dartmouth College DETECTION OF GENE SEQUENCES IN BIOLOGICAL LIQUIDS
GB9210176D0 (en) * 1992-05-12 1992-06-24 Cemu Bioteknik Ab Chemical method
US5981176A (en) * 1992-06-17 1999-11-09 City Of Hope Method of detecting and discriminating between nucleic acid sequences
CA2115342C (en) * 1992-06-17 2003-08-26 Robert B. Wallace A method of detecting and discriminating between nucleic acid sequences
DE69232846T2 (en) * 1992-06-17 2003-08-21 City Of Hope, Duarte METHOD FOR DETECTING AND DIFFERENTIATING NUCLEIC ACID SEQUENCES
US5710028A (en) * 1992-07-02 1998-01-20 Eyal; Nurit Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor
ATE231881T1 (en) * 1992-07-02 2003-02-15 Savyon Diagnostics Ltd METHOD FOR SIMPLE NUCLEOTIDE PRIMER EXTENSION FOR DETECTING SPECIFIC ALLELES AND KIT SUITABLE THEREFOR
US6153379A (en) * 1993-06-22 2000-11-28 Baylor College Of Medicine Parallel primer extension approach to nucleic acid sequence analysis
US7001722B1 (en) 1993-06-22 2006-02-21 Baylor College Of Medicine Parallel primer extension approach to nucleic acid sequence analysis
CA2111503A1 (en) * 1993-12-15 1995-06-16 Mcgill University Apolipoprotein e polymorphism and alzheimer's disease
US6428955B1 (en) 1995-03-17 2002-08-06 Sequenom, Inc. DNA diagnostics based on mass spectrometry
GB9507238D0 (en) 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
GB9517914D0 (en) * 1995-09-02 1995-11-01 Tepnel Medical Ltd Identification of bases in nucleic acid sequences
NO954667D0 (en) * 1995-11-17 1995-11-17 Dagfinn Oegreid Method for detecting Ki-ras mutations
WO1997035033A1 (en) 1996-03-19 1997-09-25 Molecular Tool, Inc. Method for determining the nucleotide sequence of a polynucleotide
EP0933431A1 (en) * 1996-07-11 1999-08-04 Wakunaga Pharmaceutical Co., Ltd. Method for examining nucleic acids and examination kits
EP2264045B1 (en) 1996-08-14 2015-10-21 Life Technologies Corporation Stable compositions for nucleic acid amplification and sequencing
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
US6140053A (en) 1996-11-06 2000-10-31 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US5945284A (en) * 1997-05-27 1999-08-31 The Perkin-Elmer Corporation Length determination of nucleic acid repeat sequences by discontinuous primer extension
US6309829B1 (en) 1997-05-27 2001-10-30 Pe Corporation (Ny) Length determination of nucleic acid repeat sequences by discontinuous primer extension
US6566101B1 (en) 1997-06-16 2003-05-20 Anthony P. Shuber Primer extension methods for detecting nucleic acids
US5888778A (en) * 1997-06-16 1999-03-30 Exact Laboratories, Inc. High-throughput screening method for identification of genetic mutations or disease-causing microorganisms using segmented primers
GB9715034D0 (en) * 1997-07-18 1997-09-24 Zeneca Ltd Assay
US7105353B2 (en) 1997-07-18 2006-09-12 Serono Genetics Institute S.A. Methods of identifying individuals for inclusion in drug studies
EP0892068A1 (en) * 1997-07-18 1999-01-20 Genset Sa Method for generating a high density linkage disequilibrium-based map of the human genome
US7745142B2 (en) 1997-09-15 2010-06-29 Molecular Devices Corporation Molecular modification assays
US6537751B1 (en) 1998-04-21 2003-03-25 Genset S.A. Biallelic markers for use in constructing a high density disequilibrium map of the human genome
CA2245039A1 (en) * 1998-08-13 2000-02-13 Vito Scalia Primer-specific and mispair extension assay for identifying gene variation
JP2002523111A (en) * 1998-08-28 2002-07-30 サンテック モレキュラー ダイアグノスティックス エービー Method of measuring a patient's ability to metabolize a particular drug
DE19855469C2 (en) * 1998-12-01 2001-01-11 Michael Esrich Method for determining the apolipoprotein E genotype in a human sample
US6503718B2 (en) 1999-01-10 2003-01-07 Exact Sciences Corporation Methods for detecting mutations using primer extension for detecting disease
US6280947B1 (en) 1999-08-11 2001-08-28 Exact Sciences Corporation Methods for detecting nucleotide insertion or deletion using primer extension
US6573047B1 (en) 1999-04-13 2003-06-03 Dna Sciences, Inc. Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation
WO2000063437A2 (en) 1999-04-20 2000-10-26 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
JP2001245698A (en) * 1999-11-22 2001-09-11 Xiao Bing Wang Method for detecting nucleic acid
IL149676A0 (en) 1999-12-02 2002-11-10 Dna Sciences Inc Methods and kits for determining nucleotide variations
US6518024B2 (en) 1999-12-13 2003-02-11 Motorola, Inc. Electrochemical detection of single base extension
US6762018B1 (en) 1999-12-23 2004-07-13 Tetragen Sa Analysis of nucleotide polymorphisms at a site
ATE412774T1 (en) 2000-02-16 2008-11-15 Illumina Inc PARALLEL GENOTYPING OF MULTIPLE PATIENT SAMPLES
US6355433B1 (en) 2000-06-02 2002-03-12 Dna Sciences, Inc. Determination of nucleotide sequence variations through limited primer extension
US6958214B2 (en) 2000-07-10 2005-10-25 Sequenom, Inc. Polymorphic kinase anchor proteins and nucleic acids encoding the same
SG115374A1 (en) * 2000-07-17 2005-10-28 Xiao Bing Wang Detection of sequence variation of nucleic acid by shifted termination analysis
CH699253B1 (en) * 2000-09-18 2010-02-15 Eidgenoessische Forschungsanst A method of characterizing and / or identification of genomes.
US6428964B1 (en) 2001-03-15 2002-08-06 Exact Sciences Corporation Method for alteration detection
US6803201B2 (en) 2002-01-24 2004-10-12 Stratagene Compositions and methods for polynucleotide sequence determination
JP4143756B2 (en) 2002-06-21 2008-09-03 財団法人名古屋産業科学研究所 Risk diagnosis method for myocardial infarction
DE10245145B4 (en) * 2002-09-27 2004-12-02 IPK-Institut für Pflanzengenetik und Kulturpflanzenforschung Procedure for the detection of SNPs on polydimensional microarrays
AU2003280603A1 (en) 2002-10-29 2004-05-25 Kabushiki Kaisha Dnaform Method of amplifying nucleic acid
US8206902B2 (en) 2003-12-25 2012-06-26 Riken Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same
CA2579150C (en) 2004-09-17 2014-11-25 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
US9777314B2 (en) 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
EP4230747A3 (en) 2008-03-28 2023-11-15 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
WO2012049279A1 (en) 2010-10-14 2012-04-19 Universitaet Des Saarlandes MEANS AND METHODS APPLYING SINGLE NUCLEOTIDE PRIMER EXTENSION WITH ION PAIR-, REVERSED-PHASE HPLC (SIRPH) FOR THE DIAGNOSIS OF SNPs

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0123513A1 (en) * 1983-04-22 1984-10-31 AMERSHAM INTERNATIONAL plc Method of detecting mutations in DNA
EP0317074A2 (en) * 1987-10-09 1989-05-24 Orion-Yhtymà„ Oy Hybridization method and reagent kit therefor
US4851331A (en) * 1986-05-16 1989-07-25 Allied Corporation Method and kit for polynucleotide assay including primer-dependant DNA polymerase
EP0333465A2 (en) * 1988-03-18 1989-09-20 Baylor College Of Medicine Mutation detection by competitive oligonucleotide priming
WO1989009835A1 (en) * 1988-04-08 1989-10-19 The Salk Institute For Biological Studies Ligase-based amplification method
WO1989010414A1 (en) * 1988-04-28 1989-11-02 Robert Bruce Wallace AMPLIFIED SEQUENCE POLYMORPHISMS (ASPs)
EP0357011A2 (en) * 1988-08-30 1990-03-07 Abbott Laboratories Detection and amplification of target nucleic acid sequences
EP0370694A2 (en) * 1988-11-21 1990-05-30 Eastman Kodak Company Diagnostic kit and method using a solid phase capture means for detecting nucleic acids
EP0371437A2 (en) * 1988-11-29 1990-06-06 Orion-Yhtymà„ Oy Method and reagent combination for determining nucleotide sequences
WO1990009455A1 (en) * 1989-02-13 1990-08-23 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
WO1990011372A1 (en) * 1989-03-21 1990-10-04 Collaborative Research, Inc. Multiplex dna diagnostic test
EP0332435B1 (en) * 1988-03-10 1992-04-22 Zeneca Limited Method of detecting nucleotide sequences

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU622104B2 (en) * 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0123513A1 (en) * 1983-04-22 1984-10-31 AMERSHAM INTERNATIONAL plc Method of detecting mutations in DNA
US4851331A (en) * 1986-05-16 1989-07-25 Allied Corporation Method and kit for polynucleotide assay including primer-dependant DNA polymerase
EP0317074A2 (en) * 1987-10-09 1989-05-24 Orion-Yhtymà„ Oy Hybridization method and reagent kit therefor
EP0332435B1 (en) * 1988-03-10 1992-04-22 Zeneca Limited Method of detecting nucleotide sequences
EP0333465A2 (en) * 1988-03-18 1989-09-20 Baylor College Of Medicine Mutation detection by competitive oligonucleotide priming
WO1989009835A1 (en) * 1988-04-08 1989-10-19 The Salk Institute For Biological Studies Ligase-based amplification method
WO1989010414A1 (en) * 1988-04-28 1989-11-02 Robert Bruce Wallace AMPLIFIED SEQUENCE POLYMORPHISMS (ASPs)
EP0357011A2 (en) * 1988-08-30 1990-03-07 Abbott Laboratories Detection and amplification of target nucleic acid sequences
EP0370694A2 (en) * 1988-11-21 1990-05-30 Eastman Kodak Company Diagnostic kit and method using a solid phase capture means for detecting nucleic acids
EP0371437A2 (en) * 1988-11-29 1990-06-06 Orion-Yhtymà„ Oy Method and reagent combination for determining nucleotide sequences
WO1990009455A1 (en) * 1989-02-13 1990-08-23 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
WO1990011372A1 (en) * 1989-03-21 1990-10-04 Collaborative Research, Inc. Multiplex dna diagnostic test

Cited By (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7329496B2 (en) 1990-12-06 2008-02-12 Affymetrix, Inc. Sequencing of surface immobilized polymers utilizing microflourescence detection
US7851146B2 (en) 1993-12-10 2010-12-14 California Institute Of Technology Nucleic acid mediated electron transfer
US7419787B2 (en) 1995-03-17 2008-09-02 Sequenom, Inc. Mass spectrometric methods for detecting mutations in a target nucleic acid
US7074563B2 (en) 1995-03-17 2006-07-11 Sequenom, Inc. Mass spectrometric methods for detecting mutations in a target nucleic acid
US6949633B1 (en) 1995-05-22 2005-09-27 Sequenom, Inc. Primers useful for sizing nucleic acids
US7384749B2 (en) 1996-11-05 2008-06-10 Clinical Micro Sensors, Inc. Electrodes linked via conductive oligomers to nucleic acids
US7125668B2 (en) 1996-11-05 2006-10-24 Clinical Micro Sensors, Inc. Electrodes linked via conductive oligomers to nucleic acids
US7056669B2 (en) 1996-11-05 2006-06-06 Clinical Micro Sensors, Inc. AC methods for the detection of nucleic acids
US7198893B1 (en) 1996-11-06 2007-04-03 Sequenom, Inc. DNA diagnostics based on mass spectrometry
US7501251B2 (en) 1996-11-06 2009-03-10 Sequenom, Inc. DNA diagnostics based on mass spectrometry
USRE44693E1 (en) 1996-11-06 2014-01-07 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US7632651B2 (en) 1997-09-15 2009-12-15 Mds Analytical Technologies (Us) Inc. Molecular modification assays
US6982431B2 (en) 1998-08-31 2006-01-03 Molecular Devices Corporation Sample analysis systems
US9279148B2 (en) 1999-04-20 2016-03-08 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US9441267B2 (en) 1999-04-20 2016-09-13 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US7070921B2 (en) 2000-04-28 2006-07-04 Molecular Devices Corporation Molecular modification assays
US9243284B2 (en) 2000-12-01 2016-01-26 Life Technologies Corporation Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis
US7432342B2 (en) 2002-05-03 2008-10-07 Sequenom, Inc. Kinase anchor protein muteins, peptides thereof and related documents
US9588051B2 (en) 2004-09-17 2017-03-07 Pacific Biosciences Of California, Inc. Apparatus and method for performing nucleic acid analysis
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence

Also Published As

Publication number Publication date
ES2072235T3 (en) 1999-07-16
ATE180019T1 (en) 1999-05-15
GR950300047T1 (en) 1995-07-31
AU642709B2 (en) 1993-10-28
EP0648280A1 (en) 1995-04-19
NO923116L (en) 1992-08-10
NO311529B1 (en) 2001-12-03
WO1991013075A2 (en) 1991-09-05
NZ237134A (en) 1992-06-25
DE648280T1 (en) 1995-11-30
IL97222A0 (en) 1992-05-25
CA2071537A1 (en) 1991-08-17
FI102297B1 (en) 1998-11-13
JPH05504477A (en) 1993-07-15
FI923653A0 (en) 1992-08-14
EP0648280B1 (en) 1999-05-12
GR3030790T3 (en) 1999-11-30
NO923116D0 (en) 1992-08-10
HU211058B (en) 1995-10-30
IE910525A1 (en) 1991-08-28
ES2072235T1 (en) 1995-07-16
ZA911152B (en) 1991-11-27
DK0648280T3 (en) 1999-11-01
CA2071537C (en) 2003-02-11
PT96776B (en) 1998-05-29
FI102297B (en) 1998-11-13
DE69131233T2 (en) 1999-11-04
IL97222A (en) 1995-08-31
FI923653L (en) 1992-08-14
HU910516D0 (en) 1991-09-30
PT96776A (en) 1991-10-31
DE69131233D1 (en) 1999-06-17
AU7235191A (en) 1991-09-18
HUT61330A (en) 1992-12-28
JP2786011B2 (en) 1998-08-13

Similar Documents

Publication Publication Date Title
WO1991013075A3 (en) Method and reagent for determining specific nucleotide variations
ATE142272T1 (en) NUCLEIC ACID AMPLIFICATION USING A SINGLE PRIMER
BR8901136A (en) PROCESS TO DETECT THE PRESENCE OR ABSENCE OF AT LEAST ONE VARIANT NUCLEOTIDE IN ONE OR MORE NUCLEIC ACIDS, SEQUENCE OF NUCLEOTIDEOS FOR USE IN THE PROCESS AND CASE TO CARRY OUT THAT DETECTION
WO1990006042A3 (en) Detection and quantitative determination of rna and dna
WO2004013357A3 (en) Nucleotide sequences specific to francisella tularensis and methods for the detection of francisella tularensis
CA2377508A1 (en) Pcr primer constructs for use in an integrated signalling system
EP0382433A3 (en) Detection of nucleic acid sequences using fluorescence polarisation
CA2182517A1 (en) Ligase/polymerase-mediated primer extension of single nucleotide polymorphisms and its use in genetic analysis
DK144886A (en) PROCEDURE FOR MULTIPLICATION OF A SPECIFIC NUCLEIC ACID SEQUENCE
WO1998041650A3 (en) Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample
FI904494A0 (en) DIAGNOSIS-OCH AMPLIFIERINGSFOERFARANDEN FOER ELIMINERING AV ETT PRIMER-MAOL-OPAR I PRIMERNS 3'-AENDA.
FI946127A0 (en) Methods for elongating a primer with a nucleotide to detect specific alleles, and test packaging for performing these methods
WO1999024606A3 (en) Specific and sensitive nucleic acid detection method
CA2303414A1 (en) Non-competitive co-amplification methods
EP0803578A3 (en) Kit for amplification and detection of nucleic acid of cytomegalovirus (cmv) utilizing a nucleic acid sequence (ss2.7)
SG153624A1 (en) Isometric primer extension method and kit for detection and quantification of specific nucleic acid
DE59914868D1 (en) DNA detection via a strand-annealing complex
WO1998026097A3 (en) NUCLEIC ACID PRIMERS AND PROBES FOR DETECTING $i(LEGIONELLA PNEUMOPHILA)
WO2004020603A3 (en) Analyte detection
EP0950718A4 (en) Method for detecting dna with probe pna
WO1998000565A3 (en) NUCLEIC ACID PRIMERS AND PROBES FOR DETECTING $i(MYCOPLASMA PNEUMONIAE)
WO2004020604A3 (en) Allele specific primer extension
CA2211165A1 (en) Methods for sensitive detection of reverse transcriptase

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU CA FI JP NO

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH DE DK ES FR GB GR IT LU NL SE

AK Designated states

Kind code of ref document: A3

Designated state(s): AU CA FI JP NO

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH DE DK ES FR GB GR IT LU NL SE

WWE Wipo information: entry into national phase

Ref document number: 2071537

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 923653

Country of ref document: FI

WWE Wipo information: entry into national phase

Ref document number: 1991903942

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1991903942

Country of ref document: EP

WWG Wipo information: grant in national office

Ref document number: 923653

Country of ref document: FI

WWG Wipo information: grant in national office

Ref document number: 1991903942

Country of ref document: EP