WO1993009222A3 - Transfection of vertebrate cells e.g. by homologous recombination - Google Patents

Transfection of vertebrate cells e.g. by homologous recombination Download PDF

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Publication number
WO1993009222A3
WO1993009222A3 PCT/US1992/009627 US9209627W WO9309222A3 WO 1993009222 A3 WO1993009222 A3 WO 1993009222A3 US 9209627 W US9209627 W US 9209627W WO 9309222 A3 WO9309222 A3 WO 9309222A3
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WO
WIPO (PCT)
Prior art keywords
cells
product
transfected
primary
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1992/009627
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French (fr)
Other versions
WO1993009222A2 (en
Inventor
Richard F Selden
Michael W Heartlein
Douglas A Treco
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shire Human Genetics Therapies Inc
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Transkaryotic Therapies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27419842&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1993009222(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Transkaryotic Therapies Inc filed Critical Transkaryotic Therapies Inc
Priority to DE69221168T priority Critical patent/DE69221168T2/en
Priority to CA002122991A priority patent/CA2122991A1/en
Priority to AU30698/92A priority patent/AU669499B2/en
Priority to JP50876793A priority patent/JP3564126B2/en
Priority to EP92924367A priority patent/EP0649464B1/en
Publication of WO1993009222A2 publication Critical patent/WO1993009222A2/en
Publication of WO1993009222A3 publication Critical patent/WO1993009222A3/en
Anticipated expiration legal-status Critical
Priority to GR970402700T priority patent/GR3025054T3/en
Ceased legal-status Critical Current

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6901—Conjugates being cells, cell fragments, viruses, ghosts, red blood cells or viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61P37/00—Drugs for immunological or allergic disorders
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    • A61P37/02—Immunomodulators
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/06—Drugs for disorders of the endocrine system of the anterior pituitary hormones, e.g. TSH, ACTH, FSH, LH, PRL, GH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/18—Drugs for disorders of the endocrine system of the parathyroid hormones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
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    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
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    • C07—ORGANIC CHEMISTRY
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    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
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    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian
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    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
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    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

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Abstract

The present invention relates to transfected primary and secondary somatic cells of vertebrate origin, particularly mammalian origin, transfected with exogenous genetic material (DNA) which encodes a desired (e.g., a therapeutic) product or is itself a desired (e.g., therapeutic) product, methods by which primary and secondary cells are transfected to include exogenous genetic material, including DNA targeting by homologous recombination, methods of producing clonal cell strains or heterogenous cell strains, methods of gene therapy in which the transfected primary or secondary cells are used, and methods of producing antibodies using the transfected primary or secondary cells. The present invention includes primary and secondary somatic cells, such as fibroblasts, keratinocytes, epithelial cells, endothelial cells, glial cells, neural cells, formed elements of the blood, muscle cells, other somatic cells which can be cultured and somatic cell precursors, which have been transfected with exogenous DNA which is stably integrated into their genomes or is expressed in the cells episomally. The exogenous DNA either encodes a product, such as a translational product (e.g., a protein) or a transcriptional product (e.g., a ribozyme or an anti-sense nucleic acid sequence) which is a therapeutic product or is itself a therapeutic product (e.g., DNA which binds to a cellular regulatory protein or alters gene expression).
PCT/US1992/009627 1991-11-05 1992-11-05 Transfection of vertebrate cells e.g. by homologous recombination Ceased WO1993009222A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
DE69221168T DE69221168T2 (en) 1991-11-05 1992-11-05 TRANSFECTION OF VERTEBRATE CELLS FOR EXAMPLE BY HOMOLOGOUS RECOMBINATION
CA002122991A CA2122991A1 (en) 1991-11-05 1992-11-05 Transfection of vertebrate cells by homologous recombination
AU30698/92A AU669499B2 (en) 1991-11-05 1992-11-05 Transfection of vertebrate cells e.g. by homologous recombination
JP50876793A JP3564126B2 (en) 1991-11-05 1992-11-05 Transfection of vertebrate cells by homologous recombination
EP92924367A EP0649464B1 (en) 1991-11-05 1992-11-05 Transfection of vertebrate cells e.g. by homologous recombination
GR970402700T GR3025054T3 (en) 1991-11-05 1997-10-15 Transfection of vertebrate cells e.g. by homologous recombination.

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US78784091A 1991-11-05 1991-11-05
US78918891A 1991-11-05 1991-11-05
US787,840 1991-11-05
US789,188 1991-11-05
US91153392A 1992-07-10 1992-07-10
US911,533 1992-07-10

Publications (2)

Publication Number Publication Date
WO1993009222A2 WO1993009222A2 (en) 1993-05-13
WO1993009222A3 true WO1993009222A3 (en) 1993-10-28

Family

ID=27419842

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1992/009627 Ceased WO1993009222A2 (en) 1991-11-05 1992-11-05 Transfection of vertebrate cells e.g. by homologous recombination

Country Status (18)

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US (7) US5994127A (en)
EP (3) EP0750044B1 (en)
JP (3) JP3564126B2 (en)
KR (1) KR100339316B1 (en)
AT (2) ATE155810T1 (en)
AU (1) AU669499B2 (en)
CA (1) CA2122991A1 (en)
DE (2) DE69221168T2 (en)
DK (2) DK0649464T3 (en)
ES (2) ES2106891T3 (en)
GR (1) GR3025054T3 (en)
IL (1) IL103649A0 (en)
MX (1) MX9206376A (en)
MY (1) MY119132A (en)
NZ (1) NZ245015A (en)
PT (1) PT101031B (en)
SG (1) SG44652A1 (en)
WO (1) WO1993009222A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7255874B1 (en) 2001-12-21 2007-08-14 Closure Medical Corporation Biocompatible polymers and adhesives: compositions, methods of making and uses related thereto
US8895050B2 (en) 2003-08-06 2014-11-25 Senomyx, Inc. Flavors, flavor modifiers, tastants, taste enhancers, umami or sweet tastants, and/or enhancers and use thereof

Families Citing this family (168)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7094400B1 (en) * 1987-05-01 2006-08-22 Shire Human Genetic Therapies, Inc. Transkaryotic implantation
US6048729A (en) * 1987-05-01 2000-04-11 Transkaryotic Therapies, Inc. In vivo protein production and delivery system for gene therapy
US5538722A (en) * 1989-06-13 1996-07-23 Stanford University Isolation, growth, differentiation and genetic engineering of human muscle cells
ATE174058T1 (en) * 1989-11-06 1998-12-15 Cell Genesys Inc PRODUCTION OF PROTEINS USING HOMOLOGOUS RECOMBINATION
HU217212B (en) 1989-12-22 1999-12-28 Applied Research Systems, ARS Holding M.V. Modifying the expression of endogenous genes with a regulatory element
US6270989B1 (en) 1991-11-05 2001-08-07 Transkaryotic Therapies, Inc. Protein production and delivery
US5641670A (en) * 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
US5968502A (en) 1991-11-05 1999-10-19 Transkaryotic Therapies, Inc. Protein production and protein delivery
US6692737B1 (en) 1991-11-05 2004-02-17 Transkaryotic Therapies, Inc. In vivo protein production and delivery system for gene therapy
US6063630A (en) 1991-11-05 2000-05-16 Transkaryotic Therapies, Inc. Targeted introduction of DNA into primary or secondary cells and their use for gene therapy
PT101031B (en) * 1991-11-05 2002-07-31 Transkaryotic Therapies Inc PROCESS FOR THE SUPPLY OF PROTEINS BY GENETIC THERAPY
FR2686899B1 (en) * 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa NOVEL BIOLOGICALLY ACTIVE POLYPEPTIDES, THEIR PREPARATION AND PHARMACEUTICAL COMPOSITIONS CONTAINING THEM.
US6531124B1 (en) 1992-07-10 2003-03-11 Transkaryotic Therapies, Inc. In vivo production and delivery of insulinotropin for gene therapy
US6670178B1 (en) 1992-07-10 2003-12-30 Transkaryotic Therapies, Inc. In Vivo production and delivery of insulinotropin for gene therapy
TW402639B (en) 1992-12-03 2000-08-21 Transkaryotic Therapies Inc Protein production and protein delivery
EP0695361B2 (en) * 1993-04-21 2009-11-11 The University Court Of The University Of Edinburgh Expression of heterologous genes according to a targeted expression profile
SE9402642D0 (en) * 1994-08-05 1994-08-05 Maria Anvret Gene Therapy
US6419920B1 (en) 1995-10-25 2002-07-16 Trans Karyotic Therapies, Inc. Hybrid matrix implants and explants
US5965125A (en) 1995-10-25 1999-10-12 Transkaryotic Therapies, Inc. Hybrid matrix implants and explants
US5869041A (en) * 1996-01-12 1999-02-09 The Miriam Hospital Delivery of bioactive compounds to an organism
WO1997029180A1 (en) * 1996-02-06 1997-08-14 Eli Lilly And Company Diabetes therapy
US6458574B1 (en) 1996-09-12 2002-10-01 Transkaryotic Therapies, Inc. Treatment of a α-galactosidase a deficiency
PT1538202E (en) 1996-09-13 2014-04-22 Shire Human Genetic Therapies Production of human alpha-galactosidase a
US6083725A (en) * 1996-09-13 2000-07-04 Transkaryotic Therapies, Inc. Tranfected human cells expressing human α-galactosidase A protein
CN1254376A (en) 1996-10-01 2000-05-24 杰龙公司 Telomerase reverse transcriptase
US5952226A (en) * 1996-11-05 1999-09-14 Modex Therapeutiques Hypoxia responsive EPO producing cells
BR9808584A (en) 1997-03-14 2000-05-23 Idec Pharma Corp Process for integrating genes at specific sites in mammalian cells via homologous recombination and vectors to obtain the same
US6172211B1 (en) 1997-07-11 2001-01-09 Boehringer Ingelheim International Gmbh Nucleic acid encoding tag7 polypeptide
PT1000154E (en) 1997-07-23 2007-03-30 Roche Diagnostics Gmbh Identification of human cell lines for the production of human proteins by endogenous gene activation
US6391633B1 (en) 1997-07-23 2002-05-21 Roche Diagnostics Gmbh Production of erythropoietin by endogenous gene activation
US6548296B1 (en) 1997-07-23 2003-04-15 Roche Diagnostics Gmbh Methods for identifying human cell lines useful for endogenous gene activation, isolated human lines identified thereby, and uses thereof
WO1999005267A1 (en) * 1997-07-23 1999-02-04 Roche Diagnostics Gmbh Identification of human cell lines for the production of human proteins by endogenous gene activation
ATE341625T1 (en) * 1997-07-23 2006-10-15 Roche Diagnostics Gmbh PRODUCTION OF ERYTHROPOIETIN BY ENDOGENOUS GENE ACTIVATION WITH VIRAL PROMOTORS
US6216034B1 (en) 1997-08-01 2001-04-10 Genetronics, Inc. Method of programming an array of needle electrodes for electroporation therapy of tissue
US6241701B1 (en) 1997-08-01 2001-06-05 Genetronics, Inc. Apparatus for electroporation mediated delivery of drugs and genes
US6055453A (en) 1997-08-01 2000-04-25 Genetronics, Inc. Apparatus for addressing needle array electrodes for electroporation therapy
US6897066B1 (en) 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
US7316923B1 (en) 1997-09-26 2008-01-08 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
ZA989497B (en) † 1997-10-20 2000-04-19 Roche Diagnostics Gmbh Positive-negative selection in homologous recombination.
TR200001580T2 (en) 1997-12-03 2000-12-21 Roche Diagnostics Gmbh Erythropoietin with high specific activity
US6541623B1 (en) 1998-04-03 2003-04-01 Hyseq, Inc. Interleukin—1 receptor antagonist and uses thereof
US6294655B1 (en) 1998-04-03 2001-09-25 Hyseq, Inc. Anti-interleukin-1 receptor antagonist antibodies and uses thereof
US6337072B1 (en) 1998-04-03 2002-01-08 Hyseq, Inc. Interleukin-1 receptor antagonist and recombinant production thereof
US6426191B1 (en) 1998-04-03 2002-07-30 Hyseq, Inc. Assays involving an IL-1 receptor antagonist
US6893838B1 (en) 1998-04-07 2005-05-17 Genetics Institute Llc DADD, death activator death domain protein
EP1071768B1 (en) * 1998-04-24 2004-12-29 Transkaryotic Therapies, Inc. Delivery of therapeutic proteins via implantation of genetically modified cells in the omentum
US6537806B1 (en) * 1998-06-02 2003-03-25 University Of Washington Compositions and methods for treating diabetes
ATE369877T1 (en) * 1998-06-26 2007-09-15 Genetronics Inc SYNERGISM OF PHOTODYNAMIC AND ELECTROPERMEATION EFFECTS ON CELL VITALITY AS A NEW CYTOTOXIC AGENT
EP2428250A1 (en) 1998-07-13 2012-03-14 Genetronics, Inc. Skin and muscle-targeted gene therapy by pulsed electrical field
WO2000002620A1 (en) 1998-07-13 2000-01-20 Genetronics, Inc. Method and apparatus for electrically assisted topical delivery of agents for cosmetic applications
US6678556B1 (en) 1998-07-13 2004-01-13 Genetronics, Inc. Electrical field therapy with reduced histopathological change in muscle
US7922709B2 (en) 1998-07-13 2011-04-12 Genetronics, Inc. Enhanced delivery of naked DNA to skin by non-invasive in vivo electroporation
WO2000032750A1 (en) 1998-11-24 2000-06-08 Regents Of The University Of Minnesota Transgenic circulating endothelial cells
DE19857609A1 (en) 1998-12-14 2000-06-15 Hannelore Ehrenreich Use of erythropoietin for the treatment of human cerebral ischemia
ATE377077T1 (en) 1999-04-28 2007-11-15 Genetics Inst Llc HUMAN GIL-19/AE289 PROTEINS AND POLYNUCLEOTIDES CODING THEREOF
EP1054018B1 (en) 1999-05-18 2009-01-28 Dyax Corp. Fab fragment libraries and methods for their use
DE60040678D1 (en) 1999-09-29 2008-12-11 Gen Hospital Corp Parathyroid hormon (pth) polypeptidderivate
JP2003514532A (en) * 1999-11-19 2003-04-22 トランスカーヨティック セラピーズ インコーポレイテッド Nucleic acid constructs for optimizing product yield
US6951839B1 (en) 1999-11-30 2005-10-04 Curis, Inc. Methods and compositions for regulating lymphocyte activity
US8168178B2 (en) 1999-11-30 2012-05-01 Curis, Inc. Methods and compositions for regulating lymphocyte activity
AU1647501A (en) 1999-12-10 2001-06-18 Cytos Biotechnology Ag Replicon based activation of endogenous genes
TW201006846A (en) 2000-03-07 2010-02-16 Senomyx Inc T1R taste receptor and genes encidung same
EP1728862A1 (en) 2000-03-16 2006-12-06 Genetica, Inc. Methods and compositions for RNA interference
US20030084471A1 (en) 2000-03-16 2003-05-01 David Beach Methods and compositions for RNA interference
EP1274720A4 (en) * 2000-04-12 2004-08-18 Human Genome Sciences Inc ALBUMIN FUSED PROTEINS
US20050100991A1 (en) * 2001-04-12 2005-05-12 Human Genome Sciences, Inc. Albumin fusion proteins
US6686188B2 (en) * 2000-05-26 2004-02-03 Amersham Plc Polynucleotide encoding a human myosin-like polypeptide expressed predominantly in heart and muscle
US6656700B2 (en) * 2000-05-26 2003-12-02 Amersham Plc Isoforms of human pregnancy-associated protein-E
US20040204379A1 (en) * 2000-06-19 2004-10-14 Cheng Seng H. Combination enzyme replacement, gene therapy and small molecule therapy for lysosomal storage diseases
KR100407792B1 (en) * 2000-08-02 2003-12-01 한국생명공학연구원 Preparation method of recombinant protein by use of human glucagon-derived peptide analogue as a fusion expression partner
US20070135345A1 (en) * 2000-09-18 2007-06-14 Henriksen Dennis B Use of GLP-2 for the treatment or prevention, of bone-related disorders
US20080249016A1 (en) * 2000-09-18 2008-10-09 Sanos Bioscience A/S Use of GLP-2 in a combination treatment for bone-related disorders
US7371721B2 (en) * 2000-09-18 2008-05-13 Sanos Bioscience A/S Use of GLP-2 and related compounds for the treatment, prevention, diagnosis, and prognosis of bone-related disorders and calcium homeostasis related syndromes
WO2002022151A2 (en) 2000-09-18 2002-03-21 Osteometer Bio Tech A/S Use of glp-1 and flp-2 peptides for treatment of bone disorders
US20020123474A1 (en) * 2000-10-04 2002-09-05 Shannon Mark E. Human GTP-Rho binding protein2
DE60144439D1 (en) 2000-12-20 2011-05-26 Hoffmann La Roche CONJUGATES OF ERYTHROPOIETIN (EPO) WITH POLYETHYLENE GLYCOL (PEG)
PA8536201A1 (en) 2000-12-29 2002-08-29 Kenneth S Warren Inst Inc PROTECTION AND IMPROVEMENT OF CELLS, FABRICS AND ORGANS RESPONDING TO Erythropoietin
TW201022287A (en) 2001-01-03 2010-06-16 Senomyx Inc T1R taste receptors and genes encoding same
WO2002072790A2 (en) 2001-03-14 2002-09-19 Myriad Genetics, Inc Tsg101-gag interaction and use thereof
US7883856B2 (en) 2001-04-05 2011-02-08 Senomyx Inc. Identification of bitter ligands that specifically activate human T2R receptors and related assays for identifying human bitter taste modulators
WO2002097038A2 (en) * 2001-05-25 2002-12-05 Human Genome Sciences, Inc. Chemokine beta-1 fusion proteins
EP2003451B1 (en) 2001-06-26 2012-05-30 Senomyx, Inc. T1r1-t1r3 hetero-oligomeric umami taste receptors and cell lines that express said receptors and use thereof for identification of umami taste compounds
US20040204669A1 (en) * 2001-07-05 2004-10-14 Hofmann Gunter A. Apparatus for electroporation mediated delivery for drugs and genes
JP4551656B2 (en) 2001-07-10 2010-09-29 セノミックス インコーポレイテッド Use of specific T2R taste receptors to identify compounds that block bitterness
CN1363654A (en) * 2001-07-19 2002-08-14 上海华谊生物技术有限公司 Genetically engineered bacteria and process for preparing insulinotropic hormone secretion peptide GLP-1 (7-36)
AU2002322720B2 (en) * 2001-07-25 2008-11-13 Raptor Pharmaceutical Inc. Compositions and methods for modulating blood-brain barrier transport
US6867189B2 (en) * 2001-07-26 2005-03-15 Genset S.A. Use of adipsin/complement factor D in the treatment of metabolic related disorders
US20040078837A1 (en) * 2001-08-02 2004-04-22 Shannon Mark E. Four human zinc-finger-containing proteins: MDZ3, MDZ4, MDZ7 and MDZ12
US6642003B2 (en) 2001-08-02 2003-11-04 Cedars-Sinai Medical Center Human glucose-dependent insulin-secreting cell line
US20040143104A1 (en) * 2001-08-08 2004-07-22 Wadsworth Samuel C. Methods of treating diabetes and other blood sugar disorders
MXPA04004534A (en) * 2001-11-16 2004-08-11 Childrens Medical Center Augmentation of organ function.
PT1463751E (en) 2001-12-21 2013-08-26 Human Genome Sciences Inc ALBUMINE FUSION PROTEINS
EP1463752A4 (en) * 2001-12-21 2005-07-13 Human Genome Sciences Inc ALBUMIN FUSION PROTEINS
CN1296485C (en) 2002-01-17 2007-01-24 英国龙沙生物医药股份有限公司 Glutamine auxotrophic human cells capable of protein production and growth on glutamine-free media
EP1585386A4 (en) 2002-02-20 2009-09-02 American Integrated Biolog Inc Transgenic production in saliva
US7141240B2 (en) * 2002-03-12 2006-11-28 Cedars-Sinai Medical Center Glucose-dependent insulin-secreting cells transfected with a nucleotide sequence encoding GLP-1
BR122017016940B8 (en) 2002-04-25 2021-07-27 Transkaryotic Therapies Inc method for preparing a pharmaceutical composition comprising alpha-galactosidase a
US7374930B2 (en) * 2002-05-21 2008-05-20 Expression Genetics, Inc. GLP-1 gene delivery for the treatment of type 2 diabetes
US7459435B2 (en) 2002-08-29 2008-12-02 Hoffmann-La Roche Inc. Treatment of disturbances of iron distribution
CA2498319A1 (en) * 2002-09-09 2004-03-18 Nautilus Biotech Rational evolution of cytokines for higher stability, the cytokines and encoding nucleic acid molecules
US7459436B2 (en) 2002-11-22 2008-12-02 Hoffmann-La Roche Inc. Treatment of disturbances of iron distribution
US20050048041A1 (en) * 2003-01-13 2005-03-03 Rao Mahendra S. Persistent expression of candidate molecule in proliferating stem and progenitor cells for delivery of therapeutic products
JP2006517101A (en) * 2003-01-13 2006-07-20 エス． ラオ、マヘンドラ Sustained expression of candidate molecules in proliferating stem and progenitor cells to deliver therapeutic products
WO2004064785A2 (en) 2003-01-14 2004-08-05 Dana Farber Cancer Institute Cancer therapy sensitizer
CA2513213C (en) 2003-01-22 2013-07-30 Human Genome Sciences, Inc. Albumin fusion proteins
US7595306B2 (en) * 2003-06-09 2009-09-29 Alnylam Pharmaceuticals Inc Method of treating neurodegenerative disease
JP2007514673A (en) * 2003-12-19 2007-06-07 エフ．ホフマン−ラ　ロシュ　アーゲー Use of erythropoietin in the treatment of impaired iron distribution during chronic inflammatory bowel disease
WO2006017673A2 (en) 2004-08-03 2006-02-16 Biogen Idec Ma Inc. Taj in neuronal function
EP2338994B1 (en) 2004-09-02 2014-03-19 Yale University Regulation of oncogenes by microRNAs
AU2006247065B8 (en) 2005-05-17 2012-07-12 Amicus Therapeutics, Inc. A method for the treatment of Pompe disease using 1-deoxynojirimycin derivatives
WO2007002528A1 (en) 2005-06-23 2007-01-04 Yale University Anti-aging micrornas
AU2006287315B2 (en) 2005-09-09 2013-02-21 The Johns Hopkins University Manipulation of regulatory T cell and DC function by targeting neuritin gene using antibodies, agonists and antagonists
MX2007015931A (en) 2005-10-20 2008-04-22 Senomyx Inc Chimeric human sweet-umami and umami-sweet taste receptors.
RU2008115455A (en) 2005-11-03 2009-12-10 Рэдпойнт Био Корпорэйшн (Us) HIGH-PERFORMANCE TRPM5 ION CHANNEL SCREENING ANALYSIS
DE102006004008A1 (en) 2006-01-27 2007-08-02 Hannelore Prof. Dr. Dr. Ehrenreich Treating or preventing multiple sclerosis comprises administering erythropoietin for periods separated by intervals in which no erythropoietin is administered
US8129334B2 (en) 2006-03-31 2012-03-06 The Regents Of The University Of California Methods and compositions for treating neurodegenerative disorders and Alzheimer'S disease and improving normal memory
EP3235811B1 (en) 2006-04-21 2018-07-25 Senomyx, Inc. Process of preparing oxalamides
JP2007325571A (en) * 2006-06-09 2007-12-20 Toto Ltd Method for producing protein using recombinant mammalian cell
WO2007150069A2 (en) 2006-06-23 2007-12-27 Myriad Genetics, Inc. Dpyd gene variants and use thereof
WO2008013861A2 (en) 2006-07-27 2008-01-31 Redpoint Bio Corporation Screening assay for inhibitors of trpa1 activation by a lower alkyl phenol
EP2120998B1 (en) 2006-11-28 2013-08-07 HanAll Biopharma Co., Ltd. Modified erythropoietin polypeptides and uses thereof for treatment
CN101687009A (en) 2007-05-11 2010-03-31 宾夕法尼亚大学理事会 Method for treating skin ulcers
KR101730290B1 (en) 2007-05-11 2017-04-25 토마스 제퍼슨 유니버시티 Methods of treatment and prevention of neurodegenerative diseases and disorders
US9580688B2 (en) * 2007-06-08 2017-02-28 Wake Forest University Health Sciences Kidney structures and methods of forming the same
CA2688265C (en) * 2007-06-08 2021-02-16 Wake Forest University Health Sciences Selective cell therapy for the treatment of renal failure
US10590391B2 (en) * 2007-06-08 2020-03-17 Wake Forest University Health Sciences Selective cell therapy for the treatment of renal failure
EP2006388A1 (en) * 2007-06-19 2008-12-24 Helmholtz-Zentrum für Infektionsforschung GmbH Nucleic acid constructs for deactivation and conditional mutagenesis of genes
CL2008002053A1 (en) 2007-07-17 2009-05-22 Hoffmann La Roche Method for the purification of a monopeglated erythropoietin (epompeg) which consists of providing a solution containing mono, poly and non-peglated erythropoietin and passing it through two steps of cation exchange chromatography and a method to produce epo mpeg that includes a purification method.
CL2008002054A1 (en) 2007-07-17 2009-05-29 Hoffmann La Roche Method for the regeneration of a cation exchange chromatography column after elusion of monopeglated erythropoietin and method to obtain a monopeglated erythropoietin, incorporating the regeneration method of the cation exchange column.
WO2009010107A1 (en) 2007-07-19 2009-01-22 Hannelore Ehrenreich Use of epo receptor activation or stimulation for the improvement of the edss score in patients with multiple sclerosis
CN101784281B (en) 2007-08-01 2014-07-02 总医院有限公司 Methods of Determining G Protein-Coupled Receptor Agonists
JP4997253B2 (en) * 2007-08-10 2012-08-08 Ｔｏｔｏ株式会社 Recombinant mammalian cell, method for producing recombinant mammalian cell, and method for producing target protein
ES2545121T3 (en) 2007-08-21 2015-09-08 Senomyx, Inc. Compounds that inhibit (block) the bitter taste in compositions, and use thereof
WO2009079399A2 (en) * 2007-12-14 2009-06-25 Alnylam Pharmaceuticals, Inc. Method of treating neurodegenerative disease
EP2245145B1 (en) 2008-01-18 2016-12-14 BioMarin Pharmaceutical Inc. Manufacture of active highly phosphorylated human lysosomal sulfatase enzymes and uses thereof
US20090291073A1 (en) * 2008-05-20 2009-11-26 Ward Keith W Compositions Comprising PKC-theta and Methods for Treating or Controlling Ophthalmic Disorders Using Same
PL2342223T3 (en) 2008-09-26 2017-09-29 Ambrx, Inc. Modified animal erythropoietin polypeptides and their uses
US8110185B2 (en) 2008-10-03 2012-02-07 St. Michael's Hospital Method for preventing and treating cardiovascular diseases with BRCA1
EP2358375B1 (en) * 2008-11-12 2013-07-24 Tengion, Inc. Isolated renal cells and uses thereof
US20100178641A1 (en) * 2009-01-12 2010-07-15 Franklin David Damon Apparel fastening learning apparatus
US8211655B2 (en) 2009-02-12 2012-07-03 Discoverx Corporation Wild-type receptor assays
EP2451475A2 (en) 2009-07-06 2012-05-16 Novartis AG Self replicating rna molecules and uses thereof
EA022396B1 (en) 2009-09-23 2015-12-30 Ратиофарм Гмбх Process for the purification of recombinant human erythropoietin (epo), epo thus purified and pharmaceutical compositions comprising same
US9492508B2 (en) 2010-05-13 2016-11-15 The General Hospital Corporation Parathyroid hormone analogs and uses thereof
AR082319A1 (en) 2010-07-22 2012-11-28 Biomarin Pharm Inc PRODUCTION OF A HIGHLY ACTIVE PHOSPHORILED HUMAN N-ACETILGALACTOSAMINE-6-SULPHATASE AND ITS USES
RU2566267C2 (en) 2010-09-14 2015-10-20 Ф. Хоффманн-Ля Рош Аг Method of purifying pegylated erythropoietin
KR102302069B1 (en) 2010-11-10 2021-09-14 인리젠 Injectable formulations for organ augmentation
WO2012075337A2 (en) 2010-12-01 2012-06-07 Spinal Modulation, Inc. Directed delivery of agents to neural anatomy
WO2013003697A1 (en) 2011-06-30 2013-01-03 Trustees Of Boston University Method for controlling tumor growth, angiogenesis and metastasis using immunoglobulin containing and proline rich receptor-1 (igpr-1)
EP3854413A1 (en) 2011-07-06 2021-07-28 GlaxoSmithKline Biologicals SA Immunogenic combination compositions and uses thereof
CA2841047A1 (en) 2011-07-06 2013-01-10 Novartis Ag Immunogenic compositions and uses thereof
EP2739734A1 (en) 2011-08-03 2014-06-11 The Charlotte-Mecklenburg Hospital Authority d/b/a Carolina Healthcare System Treatment of fibrosis using microrna-19b
US20150140068A1 (en) 2012-07-06 2015-05-21 Novartis Ag Immunogenic compositions and uses thereof
EP2970420A4 (en) 2013-03-15 2016-08-17 Apotex Inc Enhanced liquid formulation stability of erythropoietin alpha through purification processing
US10196601B2 (en) 2013-08-20 2019-02-05 Lek Pharmaceuticals D.D. Cell culture medium and process for controlling α-amidation and/or C-terminal amino acid cleavage of polypeptides
KR102473092B1 (en) 2014-09-15 2022-12-01 칠드런'즈 메디컬 센터 코포레이션 Methods and compositions to increase somatic cell nuclear transfer (scnt) efficiency by removing histone h3-lysine trimethylation
MA40871A (en) 2014-10-29 2017-09-05 Novartis Ag DIRECT EXPRESSION OF ANTIBODIES
AU2017295037B2 (en) 2016-07-15 2021-07-22 F. Hoffmann-La Roche Ag Method for purifying PEGylated erythropoietin
US11123372B2 (en) 2016-07-29 2021-09-21 Prokidney Bioactive renal cells for the treatment of chronic kidney disease
JP7410860B2 (en) 2017-12-29 2024-01-10 エフ． ホフマン－ラ ロシュ アーゲー Method for providing PEGylated protein compositions
JP7137625B2 (en) 2017-12-29 2022-09-14 エフ．ホフマン－ラ ロシュ アーゲー Methods for providing PEGylated protein compositions
HRP20220388T1 (en) 2017-12-29 2022-05-27 F. Hoffmann - La Roche Ag Process for providing pegylated protein composition
KR102559149B1 (en) 2018-10-26 2023-07-24 에프. 호프만-라 로슈 아게 Multispecific antibody screening method using recombinase-mediated cassette exchange
CN115210361B (en) * 2020-03-04 2024-12-10 淋巴奥格尼克斯有限公司 Fibroblasts with enhanced erythropoietin production ability, production method, and pharmaceutical composition
WO2022159414A1 (en) 2021-01-22 2022-07-28 University Of Rochester Erythropoietin for gastroinfestinal dysfunction
WO2024091824A1 (en) 2022-10-26 2024-05-02 Ada Forsyth Institute, Inc. Differentiation and reprogramming of chondrocyte
WO2024129459A1 (en) 2022-12-16 2024-06-20 University Of Rochester Repairmen! of barrier dysfunction in esophagus
WO2025259466A1 (en) 2024-06-13 2025-12-18 University Of Rochester Gm-csf and gm-csf-induced dendritic cells enhance fracture healing and bone repair

Citations (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2159172A (en) * 1984-05-24 1985-11-27 Damon Biotech Inc Production of antibodies
WO1987000201A1 (en) * 1985-07-05 1987-01-15 Whitehead Institute For Biomedical Research Epithelial cells expressing foreign genetic material
EP0236059A2 (en) * 1986-02-27 1987-09-09 Snow Brand Milk Products Co. Ltd. Preparation of erythropoietin-producing cells and production process of erythropoietin using same
WO1988000239A1 (en) * 1986-06-30 1988-01-14 Pharmaceutical Proteins Ltd Peptide production
EP0255231A1 (en) * 1986-06-27 1988-02-03 The Board Of Regents Of The University Of Washington Human erythropoietin gene: high level expression in stably transfected mammalian cells
WO1988008306A1 (en) * 1987-05-01 1988-11-03 The General Hospital Corporation Transkaryotic implantation
WO1989001517A1 (en) * 1987-08-07 1989-02-23 Medical Res Council Vector for integration site independent gene expression in mammalian host cells
WO1990011354A1 (en) * 1989-03-20 1990-10-04 Institut Pasteur Process for the specific replacement of a copy of a gene present in the receiver genome via the integration of a gene
WO1991009955A1 (en) * 1989-12-22 1991-07-11 Applied Research Systems Ars Holding N.V. Endogenous gene expression modification with regulatory element
WO1991013151A1 (en) * 1990-02-22 1991-09-05 Biogen, Inc. Improved expression of polypeptides
EP0452894A2 (en) * 1990-04-19 1991-10-23 Consiglio Nazionale Delle Ricerche Human cells for the high expression of genes inserted in episomal recombinant DNA, their preparation and their use
WO1991019796A1 (en) * 1990-06-12 1991-12-26 Baylor College Of Medicine Method for homologous recombination in animal and plant cells
WO1992003917A1 (en) * 1990-08-29 1992-03-19 Genpharm International Homologous recombination in mammalian cells
WO1992008796A1 (en) * 1990-11-13 1992-05-29 Immunex Corporation Bifunctional selectable fusion genes
WO1992019255A1 (en) * 1991-05-06 1992-11-12 Cell Genesys, Inc. Gene manipulation and expression using genomic elements
WO1992020808A1 (en) * 1991-05-15 1992-11-26 Cell Genesys, Inc. Genomic modifications with homologous dna targeting
WO1993004169A1 (en) * 1991-08-20 1993-03-04 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4497796A (en) * 1980-03-26 1985-02-05 The Regents Of The University Of California Gene transfer in intact mammals
US4396601A (en) * 1980-03-26 1983-08-02 The Regents Of The University Of Calif. Gene transfer in intact mammals
DE3176404D1 (en) * 1980-04-22 1987-10-08 Pasteur Institut Vaccine against viral hepatitis b, method and transformed eucaryotic cells for the preparation of this vaccine
NZ210501A (en) * 1983-12-13 1991-08-27 Kirin Amgen Inc Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence
CA1215922A (en) * 1984-05-25 1986-12-30 Connaught Laboratories Limited Microencapsulation of living tissue and cells
ATE89314T1 (en) * 1985-02-13 1993-05-15 Scios Nova Inc HUMAN METALLOTHIONEIN II PROMOTOR IN MAMMALIAN EXPRESSION SYSTEMS.
US4980286A (en) * 1985-07-05 1990-12-25 Whitehead Institute For Biomedical Research In vivo introduction and expression of foreign genetic material in epithelial cells
US5118666A (en) * 1986-05-05 1992-06-02 The General Hospital Corporation Insulinotropic hormone
US5219740A (en) * 1987-02-13 1993-06-15 Fred Hutchinson Cancer Research Center Retroviral gene transfer into diploid fibroblasts for gene therapy
US5175255A (en) * 1987-03-23 1992-12-29 Amgen Inc. Methods for purification of platelet-derived growth factor
US6048729A (en) * 1987-05-01 2000-04-11 Transkaryotic Therapies, Inc. In vivo protein production and delivery system for gene therapy
US5166059A (en) * 1987-06-16 1992-11-24 The United States Of America As Represented By The Department Of Health And Human Services Gene therapy using gene fusions for genetic or acquired disorders
WO1989000239A1 (en) * 1987-07-06 1989-01-12 Komatsu Zenoah Kabushiki Kaisha Choke for engines
DE3852823T2 (en) * 1987-09-11 1995-05-24 Hughes Howard Med Inst TRANSDUCTION-CHANGED FIBROBLASTS AND THEIR USE.
US4892538A (en) * 1987-11-17 1990-01-09 Brown University Research Foundation In vivo delivery of neurotransmitters by implanted, encapsulated cells
JP2914692B2 (en) * 1987-12-11 1999-07-05 ホワイトヘツド・インスチチユート・フオー・バイオメデイカル・リサーチ Endothelial cell genetic modification
US4890286A (en) * 1987-12-11 1989-12-26 Sanyo Electric Co., Ltd. Method and apparatus for decoding error correcting code
DE3802640A1 (en) * 1988-01-29 1989-08-03 Roehm Gmbh HAIR-RESERVED AASIS PROCEDURE
EP0400047B1 (en) * 1988-02-05 1997-04-23 Whitehead Institute For Biomedical Research Modified hepatocytes and uses therefor
WO1990006997A1 (en) * 1988-12-13 1990-06-28 United States Government As Represented By The Secretary Of The Department Of Health And Human Services Genetically engineered endothelial cells and use thereof
US5082670A (en) * 1988-12-15 1992-01-21 The Regents Of The University Of California Method of grafting genetically modified cells to treat defects, disease or damage or the central nervous system
HU9203905D0 (en) * 1989-04-05 1993-04-28 Glaxo Group Ltd Method for producing ranitidine
GB8909218D0 (en) * 1989-04-22 1989-06-07 Medical Res Council Improvements in or relating to enhancers
EP0457856A4 (en) * 1989-06-13 1992-10-21 The Board Of Directors Of The Leland Stanford Junior University Isolation growth and differentiation of human muscle cells
US5399346A (en) * 1989-06-14 1995-03-21 The United States Of America As Represented By The Department Of Health And Human Services Gene therapy
US5194596A (en) * 1989-07-27 1993-03-16 California Biotechnology Inc. Production of vascular endothelial cell growth factor
US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
ATE174058T1 (en) * 1989-11-06 1998-12-15 Cell Genesys Inc PRODUCTION OF PROTEINS USING HOMOLOGOUS RECOMBINATION
US5272071A (en) * 1989-12-22 1993-12-21 Applied Research Systems Ars Holding N.V. Method for the modification of the expression characteristics of an endogenous gene of a given cell line
EP0556345B2 (en) * 1990-10-31 2005-10-12 Cell Genesys, Inc. Retroviral vectors useful for gene therapy
JPH05505944A (en) * 1990-12-26 1993-09-02 ホワイトヘッド・インスティテュート・フォー・バイオメディカル・リサーチ Modified hepatocytes and their uses
WO1992015676A1 (en) * 1991-03-08 1992-09-17 The Salk Institute For Biological Studies Somatic cell gene therapy
PT101031B (en) * 1991-11-05 2002-07-31 Transkaryotic Therapies Inc PROCESS FOR THE SUPPLY OF PROTEINS BY GENETIC THERAPY
US6054288A (en) * 1991-11-05 2000-04-25 Transkaryotic Therapies, Inc. In vivo protein production and delivery system for gene therapy
US6063630A (en) * 1991-11-05 2000-05-16 Transkaryotic Therapies, Inc. Targeted introduction of DNA into primary or secondary cells and their use for gene therapy
US6670178B1 (en) * 1992-07-10 2003-12-30 Transkaryotic Therapies, Inc. In Vivo production and delivery of insulinotropin for gene therapy
US6531124B1 (en) * 1992-07-10 2003-03-11 Transkaryotic Therapies, Inc. In vivo production and delivery of insulinotropin for gene therapy
KR960037062A (en) * 1996-08-02 1996-11-19 오동덕 Manufacturing method of therapeutic agent for purulent skin disease

Patent Citations (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2159172A (en) * 1984-05-24 1985-11-27 Damon Biotech Inc Production of antibodies
WO1987000201A1 (en) * 1985-07-05 1987-01-15 Whitehead Institute For Biomedical Research Epithelial cells expressing foreign genetic material
EP0236059A2 (en) * 1986-02-27 1987-09-09 Snow Brand Milk Products Co. Ltd. Preparation of erythropoietin-producing cells and production process of erythropoietin using same
EP0255231A1 (en) * 1986-06-27 1988-02-03 The Board Of Regents Of The University Of Washington Human erythropoietin gene: high level expression in stably transfected mammalian cells
WO1988000239A1 (en) * 1986-06-30 1988-01-14 Pharmaceutical Proteins Ltd Peptide production
WO1988008306A1 (en) * 1987-05-01 1988-11-03 The General Hospital Corporation Transkaryotic implantation
WO1989001517A1 (en) * 1987-08-07 1989-02-23 Medical Res Council Vector for integration site independent gene expression in mammalian host cells
WO1990011354A1 (en) * 1989-03-20 1990-10-04 Institut Pasteur Process for the specific replacement of a copy of a gene present in the receiver genome via the integration of a gene
WO1991009955A1 (en) * 1989-12-22 1991-07-11 Applied Research Systems Ars Holding N.V. Endogenous gene expression modification with regulatory element
WO1991013151A1 (en) * 1990-02-22 1991-09-05 Biogen, Inc. Improved expression of polypeptides
EP0452894A2 (en) * 1990-04-19 1991-10-23 Consiglio Nazionale Delle Ricerche Human cells for the high expression of genes inserted in episomal recombinant DNA, their preparation and their use
WO1991019796A1 (en) * 1990-06-12 1991-12-26 Baylor College Of Medicine Method for homologous recombination in animal and plant cells
WO1992003917A1 (en) * 1990-08-29 1992-03-19 Genpharm International Homologous recombination in mammalian cells
WO1992008796A1 (en) * 1990-11-13 1992-05-29 Immunex Corporation Bifunctional selectable fusion genes
WO1992019255A1 (en) * 1991-05-06 1992-11-12 Cell Genesys, Inc. Gene manipulation and expression using genomic elements
WO1992020808A1 (en) * 1991-05-15 1992-11-26 Cell Genesys, Inc. Genomic modifications with homologous dna targeting
WO1993004169A1 (en) * 1991-08-20 1993-03-04 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs

Non-Patent Citations (27)

* Cited by examiner, † Cited by third party
Title
Biochimica et biophysica acta, Volume, 1992, D C Chang et al., "High efficiency gene transfection by electroporation using a radio-frequency electric field" *
Biochimie, Volume 73, 1991, R Fishel et al., "Biochemical studies of homologous and nonhomologous recombination in human cells" *
Blood, Volume 77, No. 2, January 1991, S Imagawa et al., "Regulatory Elements of the Erythropoietin Gene" *
Bone Marrow Purging and Processing, Volume, 1990, A Keating et al., "GENE TRANSFER BY ELECTROPORATION: A MODEL FOR GENE THERAPY" *
Dialog Information Service, file 154, Medline, Dialog accession no. 07244882, Medline accession no.90151882, Corey CA et al: "Erythropoiesis in murine long-term marrow cultures following transfer of the erythropoietin cDNA into marrow stromal cells", Exp Hematol Mar 1990, 18 (3) p201-4 *
Dialog Information Service, file 155, Medline, Dialog accession no. 05976989, Medline accession no.86277989, Drucker DJ et al: "Cell-specific post- translational processing of preproglucagon expressedfrom a metallothionein-glucagon fusion gene", J Biol Chem Jul 25 1986, 261 (21) p9637-43 *
Dialog Information Service, file 155, Medline, Dialog accession no. 07523611, Medline accession no.91042611, Lee YC et al: "Glucagon gene 3'-flanking sequences direct formation of proglucagon messenger RNA 3'-ends in islet and nonislet cells lines", Mol Endocrinol Jun 1990, 4 (6) p800-6 *
Dialog Information Service, file 155, Medline, Dialog accession no. 07638459, Medline accession no.91157459, Smith BR: "Regulation of hematopoesis", Yale J Biol Med Sep-Oct 1990, 63 (5) p371-80 *
Dialog Information Service, file 55, BIOSIS, Dialog accession no. 7115105, BIOSIS no. 88037850, Kramerova I A et al: "EXPRESSION OF THE CLONED HUMANERYTHROPOIETIN GENE IN CHO CELLS KRAMEROVA I A", BIOPOLIM KLETKA 5 (2). 1989. 47-51 *
DNA AND CELL BIOLOGY, Volume 9, No. 7, 1990, A M Sittler et al., "Tissue-Specific Expression of the Rat Growth Hormone Gene Is Due to the Interaction of Multiple Promoter, Not Enhancer, Elements" *
MOLECULAR AND CELLULAR BIOLOGY, Volume 11, No. 6, June 1991, S D Lupton et al., "Dominant Positive and Negative Selection Using a Hygromycin Phosphotransferase-Thymidine Kinase Fusion Gene" *
Nature, Volume 311, September 1984, R E Hammer et al., "partial correction of murine hereditary growth disorder by germ-line incorporation of a new gene" *
Nature, Volume 336, November 1988, S L Mansour et al., "Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes" *
Nucleic Acids Research, Volume 18, No. 12, 1990, J P Morgenstern et al., "Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line" *
NUCLEIC ACIDS RESEARCH, Volume 19, No. 14, 1991, J E Itzhaki et al, "Targeted disruption of a human interferon-inducible gene detected by secretion of human growth hormone" *
Proc. Natl. Acad. Sci., Volume 88, June 1991, R Scharfmann et al., "Long-term in vivo expression of retrovirus-mediated gene transfer in mouse fibroblast implants" *
Proc. Natl. Acad. Sci., Volume 88, May 1991, E G Shesely et al., "Correction of a human betas-globin gene by gene targeting" *
PROC.NATL.ACAD.SCI., Volume 86, January 1989, J M Sedivy et al, "Positive genetic selection for gene disruption in mammalian cells by homologous recombination" *
PROC.NATL.ACAD.SCI., Volume 88, September 1991, H Zheng et al, "Fidelity of targeted recombination in human fibroblasts and murine embryonic stem cells" *
SCIENCE, Volume 222, November 1983, R D Palmiter et al, "Metallothionein-Human GH Fusion Genes Stimulate Growth of Mice" *
SCIENCE, Volume 236, May 1987, R F Selden et al, "Implantation of Genetically Engineered Fibroblasts into Mice: Implications for Gene Therapy" *
SCIENCE, Volume 237, 1987, J R Morgan et al, "Expression of an Exogenous Growth Hormone Gene by Transplantable Human Epidermal Cells" *
SCIENCE, Volume 244, June 1989, M R Capecchi, "Altering the Genome by Homologous Recombination" *
SCIENCE, Volume 244, June 1989, Theodore Friedmann, "Progress Toward Human Gene Therapy" *
Science, Volume 254, December 1991, E Barr et al., "Systemic Delivery of Recombinant Proteins by Genetically Modified Myoblasts" *
Science, Volume 254, December 1991, J Dhawn et al., "Systemic Delivery of Human Growth Hormone by Injection of Genetically Engineered Myoblasts" *
The Journal of Biological Chemistry, Volume 266, No. 22, August 1991, G Y Wu et al., "Receptor-mediated Gene Delivery in Vivo. PARTIAL CORRECTION OF GENETIC ANALBUMINEMIA IN NAGASE RATS" *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7255874B1 (en) 2001-12-21 2007-08-14 Closure Medical Corporation Biocompatible polymers and adhesives: compositions, methods of making and uses related thereto
US8895050B2 (en) 2003-08-06 2014-11-25 Senomyx, Inc. Flavors, flavor modifiers, tastants, taste enhancers, umami or sweet tastants, and/or enhancers and use thereof

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