WO1994003616A3 - Vecteur de clonage et/ou de sequençage - Google Patents

Vecteur de clonage et/ou de sequençage Download PDF

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Publication number
WO1994003616A3
WO1994003616A3 PCT/BE1993/000051 BE9300051W WO9403616A3 WO 1994003616 A3 WO1994003616 A3 WO 1994003616A3 BE 9300051 W BE9300051 W BE 9300051W WO 9403616 A3 WO9403616 A3 WO 9403616A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleotide sequence
cloning
coding
vector
sequencing vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/BE1993/000051
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English (en)
Other versions
WO1994003616A2 (fr
Inventor
Philippe Bernard
Philippe Gabant
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universite Libre de Bruxelles ULB
Original Assignee
Universite Libre de Bruxelles ULB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universite Libre de Bruxelles ULB filed Critical Universite Libre de Bruxelles ULB
Priority to JP50484094A priority Critical patent/JP3654648B2/ja
Priority to DE69314180T priority patent/DE69314180T2/de
Priority to AU45530/93A priority patent/AU4553093A/en
Priority to EP93915577A priority patent/EP0652963B1/fr
Priority to US08/379,614 priority patent/US5910438A/en
Publication of WO1994003616A2 publication Critical patent/WO1994003616A2/fr
Publication of WO1994003616A3 publication Critical patent/WO1994003616A3/fr
Anticipated expiration legal-status Critical
Priority to US10/994,208 priority patent/US7176029B2/en
Priority to US11/842,131 priority patent/US20080299661A1/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un vecteur de clonage et/ou de séquençage (1) comprenant incorporé dans un vecteur à réplication autonome (2), au moins une séquence nucléotidique promotrice (3) et au moins une séquence nucléotidique (4) codant pour une protéine de fusion active en tant que poison; ladite séquence nucléotidique (4) étant obtenue par la fusion d'une séquence nucléotidique codante (5), comprenant plusieurs sites uniques de clonage et d'une séquence nucléotidique (6) codant pour une protéine poison. La présente invention concerne également la cellule hôte du vecteur selon l'invention.
PCT/BE1993/000051 1992-07-31 1993-08-02 Vecteur de clonage et/ou de sequençage Ceased WO1994003616A2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP50484094A JP3654648B2 (ja) 1992-07-31 1993-08-02 クローニング及び／または配列ベクター
DE69314180T DE69314180T2 (de) 1992-07-31 1993-08-02 Vektor für die klonierung und/oder sequenzierung
AU45530/93A AU4553093A (en) 1992-07-31 1993-08-02 Cloning and/or sequencing vector
EP93915577A EP0652963B1 (fr) 1992-07-31 1993-08-02 Vecteur de clonage et/ou de sequencage
US08/379,614 US5910438A (en) 1992-07-31 1993-08-02 Cloning and/or sequencing vector
US10/994,208 US7176029B2 (en) 1992-07-31 2004-11-19 Cloning and/or sequencing vector
US11/842,131 US20080299661A1 (en) 1992-07-31 2007-08-20 Cloning and/or sequencing vector

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BE9200696A BE1006085A3 (fr) 1992-07-31 1992-07-31 Vecteur de clonage.
BE9200696 1992-07-31

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US08379614 A-371-Of-International 1993-08-02
US22515298A Continuation-In-Part 1992-07-31 1998-01-04

Publications (2)

Publication Number Publication Date
WO1994003616A2 WO1994003616A2 (fr) 1994-02-17
WO1994003616A3 true WO1994003616A3 (fr) 1994-03-31

Family

ID=3886385

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/BE1993/000051 Ceased WO1994003616A2 (fr) 1992-07-31 1993-08-02 Vecteur de clonage et/ou de sequençage

Country Status (8)

Country Link
US (3) US5910438A (fr)
EP (1) EP0652963B1 (fr)
JP (1) JP3654648B2 (fr)
AU (1) AU4553093A (fr)
BE (1) BE1006085A3 (fr)
CA (1) CA2141412A1 (fr)
DE (1) DE69314180T2 (fr)
WO (1) WO1994003616A2 (fr)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE1006085A3 (fr) * 1992-07-31 1994-05-10 Univ Bruxelles Vecteur de clonage.
US7176029B2 (en) * 1992-07-31 2007-02-13 Universite Libre De Bruxelles Cloning and/or sequencing vector
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
DK0937098T3 (da) 1995-06-07 2002-12-02 Invitrogen Corp Rekombinatorisk kloning under anvendelse af gensplejsede rekombinationssites
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6964861B1 (en) 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
US5851808A (en) 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
CN101125873A (zh) * 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
US7351578B2 (en) 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
EP1025217B1 (fr) 1997-10-24 2006-10-04 Invitrogen Corporation Clonage recombinatoire au moyen d'acides nucleiques possedant des sites de recombinaison
US6022716A (en) 1998-04-10 2000-02-08 Genset Sa High throughput DNA sequencing vector
AU766257C (en) 1998-05-07 2004-06-03 Universite Libre De Bruxelles Cytotoxin-based biological containment
JP2002537800A (ja) * 1999-03-02 2002-11-12 インビトロゲン・コーポレーション 毒性遺伝子に対して耐性のある細胞およびその使用
EP2119788A1 (fr) 1999-03-02 2009-11-18 Life Technologies Corporation Compositions et procédés pour une utilisation dans le clonage combinatoire d'acides nucléiques
EP2210948A3 (fr) 1999-12-10 2010-10-06 Life Technologies Corporation Utilisation de sites à recombinaison multiples avec une spécificité unique de clonage recombinatoire
EP1111061A1 (fr) * 1999-12-20 2001-06-27 Universite Libre De Bruxelles Vecteur de selection double
US7244560B2 (en) 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6544782B1 (en) * 2000-11-13 2003-04-08 Synthegen Systems pREM: a positive selection vector system for direct PCR cloning
US8470580B2 (en) 2001-02-23 2013-06-25 Universite Libre De Bruxelles Method for the selection of recombination clones comprising a sequence encoding an antidote protein to a toxic molecule
US20050260585A1 (en) * 2002-03-19 2005-11-24 Cedric Szpirer Poison/antidote genetic systems for the selection of genetically modified eucaryote cells or organisms
TW201002122A (en) * 2002-04-12 2010-01-01 Interdigital Tech Corp Access burst detector correlator pool
JP2005532829A (ja) * 2002-07-18 2005-11-04 インヴィトロジェン コーポレーション 組換え部位を含むウイルスベクター
JP4336770B2 (ja) * 2002-07-26 2009-09-30 独立行政法人産業技術総合研究所 致死遺伝子を用いた形質転換体選択用マーカー
US9309518B2 (en) 2002-09-03 2016-04-12 Universite Libre De Bruxelles Reversible, parallel and multitask cloning method and kit
WO2004022745A2 (fr) * 2002-09-03 2004-03-18 Universite Libre De Bruxelles Procede et necessaire de clonage reversible, parallele et multitache
WO2005028615A2 (fr) * 2003-06-26 2005-03-31 Invitrogen Corporation Procedes et compositions permettant de detecter une activite de promoteur et d'exprimer des proteines de fusion
US8293503B2 (en) * 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
CA2541177A1 (fr) * 2003-10-03 2005-09-22 Promega Corporation Vecteurs pour le clonage directionnel
US8304189B2 (en) 2003-12-01 2012-11-06 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same
EP2119789A1 (fr) 2008-05-16 2009-11-18 Université Libre de Bruxelles Cellule recombinante hyperproliférative
KR101077942B1 (ko) 2009-02-05 2011-10-31 서울대학교산학협력단 식물체 일시 발현용 고처리량 벡터 및 그 용도
ES2632429T3 (es) 2012-02-14 2017-09-13 Merial, Inc. Vacunas subunitarias de rotavirus y procedimientos para la fabricación y utilización de las mismas
US10358655B1 (en) 2012-06-27 2019-07-23 University Of Utah Research Foundation Attenuated protein expression vectors and associated methods
WO2015024855A1 (fr) 2013-08-19 2015-02-26 Syngulon Sa Croissance régulée de microorganismes
US11618899B2 (en) 2016-02-09 2023-04-04 Brookhaven Science Associates, Llc Cloning and expression vectors and systems
EP3675639A4 (fr) 2017-08-31 2021-09-01 Syngulon SA Procédés et compositions de fabrication de bactériocines et de peptides antimicrobiens
US11932672B2 (en) 2017-12-19 2024-03-19 Syngulon S.A. Fermentation process
BR112020024767A2 (pt) 2018-06-06 2021-03-23 Syngulon Sa engenharia de peptídeos antimicrobianos

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987005932A1 (fr) * 1986-03-26 1987-10-08 Genexpress Aps Limitation biologique
US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US5300431A (en) * 1991-02-26 1994-04-05 E. I. Du Pont De Nemours And Company Positive selection vector for the bacteriophage P1 cloning system
BE1006085A3 (fr) 1992-07-31 1994-05-10 Univ Bruxelles Vecteur de clonage.
US7176029B2 (en) * 1992-07-31 2007-02-13 Universite Libre De Bruxelles Cloning and/or sequencing vector
JP2590761B2 (ja) * 1994-11-22 1997-03-12 日本電気株式会社 Ｔａｂ式半導体装置およびｔａｂ式半導体装置を回路基板に接続する方法
DK0937098T3 (da) * 1995-06-07 2002-12-02 Invitrogen Corp Rekombinatorisk kloning under anvendelse af gensplejsede rekombinationssites
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US5922583A (en) * 1995-10-17 1999-07-13 Biostar Inc. Methods for production of recombinant plasmids
AU766257C (en) 1998-05-07 2004-06-03 Universite Libre De Bruxelles Cytotoxin-based biological containment
US6271359B1 (en) * 1999-04-14 2001-08-07 Musc Foundation For Research Development Tissue-specific and pathogen-specific toxic agents and ribozymes
US8470580B2 (en) * 2001-02-23 2013-06-25 Universite Libre De Bruxelles Method for the selection of recombination clones comprising a sequence encoding an antidote protein to a toxic molecule
US20050260585A1 (en) * 2002-03-19 2005-11-24 Cedric Szpirer Poison/antidote genetic systems for the selection of genetically modified eucaryote cells or organisms

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
B. HENRICH AND R. PLAPP: "Use of the lysis gene of bacteriophage PhiX174 for the construction of a positive selection vector", GENE, vol. 42, no. 3, 1986, ELSEVIER PUBLISHERS, N.Y., U.S.;, pages 345 - 349 *
I. KUHN ET AL.: "Positive selection vectors utilizing lethality of the EcoRI endonuclease", GENE, vol. 42, no. 3, 1986, ELSEVIER PUBLISHERS, N.Y., U.S.;, pages 253 - 263 *
J.C. PIERCE ET AL.: "A positive selection vector for cloning high molecular weight DNA by the bacteriophage P1 system: Improved cloning efficiency", PROC. NATL. ACAD SCI., vol. 89, no. 6, 15 March 1992 (1992-03-15), NATL. ACAD SCI., WASHINGTON, DC, US;, pages 2056 - 2060 *
M. COUTURIER AND P. BERNARD: "The mini F plasmid CcdB killer protein is a poison of E.coli topoisomerase II", J. CELL. BIOCHEM. SUPPL. 0, vol. 16B, 1992, pages 104 *
P. BERNARD AND M. COUTURIER: "Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomeraseII complexes", J. MOL. BIOL., vol. 226, no. 3, 5 August 1992 (1992-08-05), ACADEMIC PRESS LIMITED, LONDON, UK;, pages 735 - 745 *
P. BERNARD AND M. COUTURIER: "The 41 carboxy-terminal residues of the miniF plasmid CcdA protein are sufficient to antagonize the killer activity of the CcdB protein", MOLECULAR & GENERAL GENETICS, vol. 226, no. 1/2, April 1991 (1991-04-01), SPRINGER INTERNATIONAL, NEW YORK, US;, pages 297 - 304 *
SAMBROOK, FRITSCH AND MANIATIS, MOLECULAR CLONING, LABORATORY MANUAL, SECOND EDITION, vol. 1-3, 1989, CSH, LONG ISLAND, NY,US;, pages 4.12 - A9-A13 *

Also Published As

Publication number Publication date
DE69314180T2 (de) 1998-03-19
US6180407B1 (en) 2001-01-30
CA2141412A1 (fr) 1994-02-17
US20080299661A1 (en) 2008-12-04
DE69314180D1 (de) 1997-10-30
EP0652963B1 (fr) 1997-09-24
EP0652963A1 (fr) 1995-05-17
BE1006085A3 (fr) 1994-05-10
WO1994003616A2 (fr) 1994-02-17
JPH08500484A (ja) 1996-01-23
AU4553093A (en) 1994-03-03
US5910438A (en) 1999-06-08
JP3654648B2 (ja) 2005-06-02

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