WO1994011507A3 - Production of monoclonal recombinant antibodies without the use of hybridomas by in vitro spleen fragment culture combined with isothermal self-sustained sequence replication of rna - Google Patents

Production of monoclonal recombinant antibodies without the use of hybridomas by in vitro spleen fragment culture combined with isothermal self-sustained sequence replication of rna Download PDF

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Publication number
WO1994011507A3
WO1994011507A3 PCT/US1993/011295 US9311295W WO9411507A3 WO 1994011507 A3 WO1994011507 A3 WO 1994011507A3 US 9311295 W US9311295 W US 9311295W WO 9411507 A3 WO9411507 A3 WO 9411507A3
Authority
WO
WIPO (PCT)
Prior art keywords
rna
hybridomas
production
recombinant antibodies
sequence replication
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1993/011295
Other languages
French (fr)
Other versions
WO1994011507A2 (en
Inventor
Thomas R Gingeras
Norman R M D Klinman
Cathy A Stillman
Phyllis-Jean Linton
Debra J Decker
Matthew C Biery
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LINTON PHYLLIS JEAN
Original Assignee
LINTON PHYLLIS JEAN
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by LINTON PHYLLIS JEAN filed Critical LINTON PHYLLIS JEAN
Priority to AU56737/94A priority Critical patent/AU5673794A/en
Priority to EP94902330A priority patent/EP0628076A1/en
Publication of WO1994011507A2 publication Critical patent/WO1994011507A2/en
Publication of WO1994011507A3 publication Critical patent/WO1994011507A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention is directed to a method for producing a recombinant protein having a preselected binding affinity and specificity for a given antigen. Generally, the method combines spleen fragment culture for the in vitro somatic mutation of B cells in response to antigen, followed by preferential amplification of RNA encoding heavy and light chains of desired antibodies produced by those B cells. The amplified RNA is then converted to DNA and incorporated into expression vectors; transfected host cells then express the antibody chains or a single chain antibody having preselected characteristics. Also disclosed are generic degenerate primer pools for the efficient amplification of RNA encoding mouse IgG.
PCT/US1993/011295 1992-11-19 1993-11-19 Production of monoclonal recombinant antibodies without the use of hybridomas by in vitro spleen fragment culture combined with isothermal self-sustained sequence replication of rna Ceased WO1994011507A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU56737/94A AU5673794A (en) 1992-11-19 1993-11-19 Production of monoclonal recombinant antibodies without the use of hybridomas by (in vitro) spleen fragment culture combined with isothermal self-sustained sequence replication of rna
EP94902330A EP0628076A1 (en) 1992-11-19 1993-11-19 PRODUCTION OF MONOCLONAL RECOMBINANT ANTIBODIES WITHOUT THE USE OF HYBRIDOMAS BY $i(IN VITRO) SPLEEN FRAGMENT CULTURE COMBINED WITH ISOTHERMAL SELF-SUSTAINED SEQUENCE REPLICATION OF RNA

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US97883592A 1992-11-19 1992-11-19
US07/978,835 1992-11-19

Publications (2)

Publication Number Publication Date
WO1994011507A2 WO1994011507A2 (en) 1994-05-26
WO1994011507A3 true WO1994011507A3 (en) 1994-07-07

Family

ID=25526436

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1993/011295 Ceased WO1994011507A2 (en) 1992-11-19 1993-11-19 Production of monoclonal recombinant antibodies without the use of hybridomas by in vitro spleen fragment culture combined with isothermal self-sustained sequence replication of rna

Country Status (3)

Country Link
EP (1) EP0628076A1 (en)
AU (1) AU5673794A (en)
WO (1) WO1994011507A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000020460A1 (en) * 1998-10-05 2000-04-13 Ludwig Institute For Cancer Research Methods for producing human tumor antigen specific antibodies
ATE551431T1 (en) * 2001-05-14 2012-04-15 Henry Hongjun Ji NEW METHOD FOR CLONING SEQUENCES OF VARIABLE DOMAIN OF THE IMMUNOLOGICAL GENE REPERTOIRE
GB0701253D0 (en) 2007-01-23 2007-02-28 Diagnostics For The Real World Nucleic acid amplification and testing
AU2011352207B2 (en) * 2010-12-31 2016-03-03 Bioatla, Llc Comprehensive monoclonal antibody generation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992008800A1 (en) * 1990-11-13 1992-05-29 Siska Diagnostics, Inc. Nucleic acid amplification by two-enzyme, self-sustained sequence replication

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992008800A1 (en) * 1990-11-13 1992-05-29 Siska Diagnostics, Inc. Nucleic acid amplification by two-enzyme, self-sustained sequence replication

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LINTON, P-J. ET AL.;: "Primary antibody-forming cells and secondary B cells are generated from separate precursor cell populations", CELL, vol. 59, 22 December 1989 (1989-12-22), CAMBRIDGE, NA US, pages 1049 - 1059 *

Also Published As

Publication number Publication date
WO1994011507A2 (en) 1994-05-26
AU5673794A (en) 1994-06-08
EP0628076A1 (en) 1994-12-14

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