WO1994017409B1 - Quantification d'analytes a l'etat de trace par electrophorese capillaire d'affinity - Google Patents

Quantification d'analytes a l'etat de trace par electrophorese capillaire d'affinity

Info

Publication number
WO1994017409B1
WO1994017409B1 PCT/US1994/000426 US9400426W WO9417409B1 WO 1994017409 B1 WO1994017409 B1 WO 1994017409B1 US 9400426 W US9400426 W US 9400426W WO 9417409 B1 WO9417409 B1 WO 9417409B1
Authority
WO
WIPO (PCT)
Prior art keywords
analyte
labelled
fragment
fab
agent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1994/000426
Other languages
English (en)
Other versions
WO1994017409A1 (fr
Filing date
Publication date
Priority claimed from US08/006,922 external-priority patent/US5348633A/en
Application filed filed Critical
Priority to AU61625/94A priority Critical patent/AU6162594A/en
Priority to JP51708594A priority patent/JP3530914B2/ja
Priority to EP94908588A priority patent/EP0680608A1/fr
Publication of WO1994017409A1 publication Critical patent/WO1994017409A1/fr
Publication of WO1994017409B1 publication Critical patent/WO1994017409B1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Abstract

L'invention se rapporte à un procédé de détection quantitative de traces d'un analyte dans un échantillon. Le procédé, dans le mode préféré de réalisation, consiste à produire un fragment Fab' d'une immunoglobuline marquée au niveau d'un groupe sulfhydryle réactif avec une couleur fluorescente; à combiner le fragment Fab' marqué avec un échantillon qui peut contenir l'analyte; à concentrer les éléments du mélange obtenu dans un champ électrique; à séparer le complexe marqué analyte/agent obtenu à partir de n'importe quel agent marqué non transformé à l'aide des procédés électrophorétiques capillaires; et à détecter le signal fluorescent du complexe marqué analyte/agent. L'invention se rapporte également à un procédé de production d'un fragment Fab' marqué qui consiste à dissocier une molécule d'immunoglobuline pour obtenir un fragment F(ab')2; à réduire les liaisons de dislufure du fragment F(ab')2 pour obtenir deux fragments Fab', chacun ayant au moins un groupe sulfhydryle réactif, libre; et à mélanger un fragment Fab' ayant au moins un grupe sulfhydryle libre avec une couleur libre pour former un fragment Fab' marqué. De préférence, avant l'étape finale de mélanger, des liaisons de disulfure intrabrins sont formées par l'oxydation dans chaque fragment Fab', produisant ainsi des fragments individuels Fab' ayant chacun un groupe sulfhydryle réactif unique. Le procédé de détection quantitative consiste également, de manière plus générale, à utiliser n'importe quel agent biospécifique pour former un complexe avec l'analyte cible.
PCT/US1994/000426 1993-01-22 1994-01-12 Quantification d'analytes a l'etat de trace par electrophorese capillaire d'affinity Ceased WO1994017409A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU61625/94A AU6162594A (en) 1993-01-22 1994-01-12 Quantifying trace analytes by affinity capillary electrophoresis
JP51708594A JP3530914B2 (ja) 1993-01-22 1994-01-12 親和結合毛細管電気泳動による微量分析物の定量
EP94908588A EP0680608A1 (fr) 1993-01-22 1994-01-12 Quantification d'analytes a l'etat de trace par electrophorese capillaire d'affinity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/006,922 US5348633A (en) 1993-01-22 1993-01-22 Method for quantitating trace amounts of an analyte in a sample by affinity capillary electrophoresis
US006,922 1993-01-22

Publications (2)

Publication Number Publication Date
WO1994017409A1 WO1994017409A1 (fr) 1994-08-04
WO1994017409B1 true WO1994017409B1 (fr) 1994-09-15

Family

ID=21723287

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1994/000426 Ceased WO1994017409A1 (fr) 1993-01-22 1994-01-12 Quantification d'analytes a l'etat de trace par electrophorese capillaire d'affinity

Country Status (5)

Country Link
US (1) US5348633A (fr)
EP (1) EP0680608A1 (fr)
JP (1) JP3530914B2 (fr)
AU (1) AU6162594A (fr)
WO (1) WO1994017409A1 (fr)

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