WO1995018377A1 - Binding assay - Google Patents

Binding assay Download PDF

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Publication number
WO1995018377A1
WO1995018377A1 PCT/GB1994/002814 GB9402814W WO9518377A1 WO 1995018377 A1 WO1995018377 A1 WO 1995018377A1 GB 9402814 W GB9402814 W GB 9402814W WO 9518377 A1 WO9518377 A1 WO 9518377A1
Authority
WO
WIPO (PCT)
Prior art keywords
binding agent
analyte
array
concentration
signal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB1994/002814
Other languages
French (fr)
Inventor
Roger Philip Ekins
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Multilyte Ltd
Original Assignee
Multilyte Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=10747224&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1995018377(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Multilyte Ltd filed Critical Multilyte Ltd
Priority to US08/663,176 priority Critical patent/US5837551A/en
Priority to DE69410636T priority patent/DE69410636T2/en
Priority to AU13218/95A priority patent/AU1321895A/en
Priority to EP95904616A priority patent/EP0736176B1/en
Priority to JP51785595A priority patent/JP3391459B2/en
Publication of WO1995018377A1 publication Critical patent/WO1995018377A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/97—Test strip or test slide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/973—Simultaneous determination of more than one analyte
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/809—Multifield plates or multicontainer arrays

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Reproductive Health (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention provides a method, device and test kit for determining the concentration of analytes in liquid samples in which the amount of binding agent having binding sites specific for a given analyte in the liquid sample is immobilised in a test zone on a solid support, the binding agent being divided into an array of spatially separated locations in the test zone. The concentration of the analyte is obtained by back-titrating the occupied binding agent with a developing agent having a marker and integrating the signal from each location in the array. The assay has the advantage of avoiding the increasing statistical errors as the area of a prior art microspot is reduced, while optimising signal-to-noise and the kinetics of the reaction between analyte and binding agent.
PCT/GB1994/002814 1993-12-24 1994-12-23 Binding assay Ceased WO1995018377A1 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US08/663,176 US5837551A (en) 1993-12-24 1994-12-23 Binding assay
DE69410636T DE69410636T2 (en) 1993-12-24 1994-12-23 BINDING
AU13218/95A AU1321895A (en) 1993-12-24 1994-12-23 Binding assay
EP95904616A EP0736176B1 (en) 1993-12-24 1994-12-23 Binding assay
JP51785595A JP3391459B2 (en) 1993-12-24 1994-12-23 Binding assay

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB939326450A GB9326450D0 (en) 1993-12-24 1993-12-24 Binding assay
GB9326450.5 1993-12-24

Publications (1)

Publication Number Publication Date
WO1995018377A1 true WO1995018377A1 (en) 1995-07-06

Family

ID=10747224

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1994/002814 Ceased WO1995018377A1 (en) 1993-12-24 1994-12-23 Binding assay

Country Status (8)

Country Link
US (1) US5837551A (en)
EP (1) EP0736176B1 (en)
JP (1) JP3391459B2 (en)
AU (1) AU1321895A (en)
DE (1) DE69410636T2 (en)
ES (1) ES2119376T3 (en)
GB (1) GB9326450D0 (en)
WO (1) WO1995018377A1 (en)

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2324601A (en) * 1997-03-03 1998-10-28 Asahi Optical Co Ltd Solid phase plate assay using an immunoreagent immobilized on particles as a detector system
US6242246B1 (en) 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
EP1715341A1 (en) * 2005-04-04 2006-10-25 Micronas Holding GmbH Method and apparatus for determining the concentrations of at least 2 ligands.
US7157234B2 (en) 1997-10-24 2007-01-02 Beckman Coulter, Inc. Detection of very low quantities of analyte bound to a solid phase
EP1965213A3 (en) * 1998-02-04 2009-07-15 Invitrogen Corporation Microarrays and uses therefor
EP2249155A2 (en) 2004-09-30 2010-11-10 IFOM Fondazione Istituto Firc di Oncologia Molecolare Cancer markers
US7855054B2 (en) 2007-01-16 2010-12-21 Somalogic, Inc. Multiplexed analyses of test samples
US8071288B2 (en) 1997-12-15 2011-12-06 Somalogic, Inc. Methods and reagents for detecting target binding by nucleic acid ligands
WO2012042213A1 (en) 2010-09-29 2012-04-05 Bath Ventures Novel interaction between staphylococcus aureus sbi and c3d proteins
US8945830B2 (en) 1997-12-15 2015-02-03 Somalogic, Inc. Multiplexed analyses of test samples
US9404919B2 (en) 2007-01-16 2016-08-02 Somalogic, Inc. Multiplexed analyses of test samples
US9910034B2 (en) 2007-11-06 2018-03-06 Ambergen, Inc. Methods and compositions for phototransfer

Families Citing this family (40)

* Cited by examiner, † Cited by third party
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US6958245B2 (en) 1996-04-25 2005-10-25 Bioarray Solutions Ltd. Array cytometry
US7144119B2 (en) * 1996-04-25 2006-12-05 Bioarray Solutions Ltd. System and method for programmable illumination pattern generation
US7041510B2 (en) 1996-04-25 2006-05-09 Bioarray Solutions Ltd. System and method for programmable illumination pattern generation
US6251691B1 (en) 1996-04-25 2001-06-26 Bioarray Solutions, Llc Light-controlled electrokinetic assembly of particles near surfaces
US6387707B1 (en) * 1996-04-25 2002-05-14 Bioarray Solutions Array Cytometry
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US20030027126A1 (en) 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
DE19715484A1 (en) * 1997-04-14 1998-10-15 Boehringer Mannheim Gmbh Procedure for applying reagent spots
DE69838067T2 (en) * 1997-05-23 2008-03-13 Bioarray Solutions Ltd. COLOR CODING AND SITU INQUIRY OF MATRIX-COUPLED CHEMICAL COMPOUNDS
US6271044B1 (en) * 1998-05-06 2001-08-07 University Of Pittsburgh Of The Commonwealth System Of Higher Education Method and kit for detecting an analyte
GB9903555D0 (en) * 1999-02-16 1999-04-07 The Technology Partnership Plc Chemical and biological assay method and apparatus
WO2001013096A1 (en) * 1999-08-13 2001-02-22 Zeptosens Ag Device and method for determining multiple analytes
CA2408291C (en) 2000-05-04 2014-07-15 Yale University High density protein arrays for screening of protein activity
EP1281063A1 (en) * 2000-05-06 2003-02-05 Zeptosens AG Grating optical waveguide structure for multi-analyte determinations and the use thereof
US7396675B2 (en) * 2000-06-02 2008-07-08 Bayer Technology Services Gmbh Kit and method for determining a plurality of analytes
US9709559B2 (en) 2000-06-21 2017-07-18 Bioarray Solutions, Ltd. Multianalyte molecular analysis using application-specific random particle arrays
AU7299301A (en) 2000-06-21 2002-01-02 Bioarray Solutions Ltd Multianalyte molecular analysis using application-specific random particle arrays
US7057704B2 (en) * 2000-09-17 2006-06-06 Bioarray Solutions Ltd. System and method for programmable illumination pattern generation
US20030045005A1 (en) * 2000-10-17 2003-03-06 Michael Seul Light-controlled electrokinetic assembly of particles near surfaces
US20040058385A1 (en) * 2000-11-17 2004-03-25 Abel Andreas Peter Kit and method for determining multiple analytes, with provisions for refrencing the density of immobilised recognition elements
US7262063B2 (en) 2001-06-21 2007-08-28 Bio Array Solutions, Ltd. Directed assembly of functional heterostructures
US20040253596A1 (en) * 2001-08-27 2004-12-16 Michael Pawlak Bioanalytical recognition surface with optimized recognition element density
ES2712173T3 (en) 2001-10-15 2019-05-09 Bioarray Solutions Ltd Multiplex analysis of polymorphic loci through simultaneous consultation and enzyme-mediated detection
US7335153B2 (en) 2001-12-28 2008-02-26 Bio Array Solutions Ltd. Arrays of microparticles and methods of preparation thereof
AU2003242251A1 (en) * 2002-05-13 2003-11-11 Zeptosens Ag Kit for assay development and serial analysis
WO2004047007A1 (en) 2002-11-15 2004-06-03 Bioarray Solutions, Ltd. Analysis, secure access to, and transmission of array images
US20040248323A1 (en) 2003-06-09 2004-12-09 Protometrix, Inc. Methods for conducting assays for enzyme activity on protein microarrays
US20050079520A1 (en) * 2003-07-21 2005-04-14 Jie Wu Multiplexed analyte detection
WO2005029705A2 (en) 2003-09-18 2005-03-31 Bioarray Solutions, Ltd. Number coding for identification of subtypes of coded types of solid phase carriers
PT1664722E (en) 2003-09-22 2011-12-28 Bioarray Solutions Ltd Surface immobilized polyelectrolyte with multiple functional groups capable of covalently bonding to biomolecules
EP1692298A4 (en) 2003-10-28 2008-08-13 Bioarray Solutions Ltd Optimization of gene expression analysis using immobilized capture probes
WO2005045060A2 (en) 2003-10-29 2005-05-19 Bioarray Solutions, Ltd. Multiplexed nucleic acid analysis by fragmentation of double-stranded dna
WO2005046859A2 (en) * 2003-11-12 2005-05-26 Bio-Rad Haifa Ltd. System and method for carrying out multiple binding reactions in an array format
US7887750B2 (en) * 2004-05-05 2011-02-15 Bayer Healthcare Llc Analytical systems, devices, and cartridges therefor
US7848889B2 (en) 2004-08-02 2010-12-07 Bioarray Solutions, Ltd. Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification
DE102004052729A1 (en) 2004-10-30 2006-05-04 Roche Diagnostics Gmbh Immunocomplex-specific antibody for zero-reduction in the detection of antigen-specifically bound antibodies of a specific immunoglobulin class in array test formats
EP1877773B1 (en) * 2005-04-26 2014-10-15 Bayer Intellectual Property GmbH Novel equipment and method for coating substrates for analyte detection by way of an affinity assay method
US8486629B2 (en) 2005-06-01 2013-07-16 Bioarray Solutions, Ltd. Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation
EP2576759A4 (en) 2010-05-25 2014-01-22 Arryx Inc Methods and apparatuses for detection of positional freedom of particles in biological and chemical analyses and applications in immunodiagnostics
US20250138006A1 (en) * 2021-08-17 2025-05-01 Technische Universiteit Eindhoven Method and system for the monitoring of an analyte of interest

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988001058A1 (en) * 1986-08-06 1988-02-11 Roger Philip Ekins Determination of analyte concentration using two labelling markers
EP0304202A1 (en) * 1987-08-06 1989-02-22 Multilyte Limited Determination of ambient concentrations of several analytes
WO1993008472A1 (en) * 1991-10-15 1993-04-29 Multilyte Limited Binding assay employing labelled reagent

Family Cites Families (6)

* Cited by examiner, † Cited by third party
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AU1944283A (en) * 1982-08-27 1984-03-29 Ekins Roger Philip Measurement of analyte concentration
US5096807A (en) * 1985-03-06 1992-03-17 Murex Corporation Imaging immunoassay detection system with background compensation and its use
US5324633A (en) * 1991-11-22 1994-06-28 Affymax Technologies N.V. Method and apparatus for measuring binding affinity
US5508200A (en) * 1992-10-19 1996-04-16 Tiffany; Thomas Method and apparatus for conducting multiple chemical assays
US5552272A (en) * 1993-06-10 1996-09-03 Biostar, Inc. Detection of an analyte by fluorescence using a thin film optical device
US5599668A (en) * 1994-09-22 1997-02-04 Abbott Laboratories Light scattering optical waveguide method for detecting specific binding events

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988001058A1 (en) * 1986-08-06 1988-02-11 Roger Philip Ekins Determination of analyte concentration using two labelling markers
EP0304202A1 (en) * 1987-08-06 1989-02-22 Multilyte Limited Determination of ambient concentrations of several analytes
WO1993008472A1 (en) * 1991-10-15 1993-04-29 Multilyte Limited Binding assay employing labelled reagent

Cited By (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2324601A (en) * 1997-03-03 1998-10-28 Asahi Optical Co Ltd Solid phase plate assay using an immunoreagent immobilized on particles as a detector system
US7157234B2 (en) 1997-10-24 2007-01-02 Beckman Coulter, Inc. Detection of very low quantities of analyte bound to a solid phase
US8071288B2 (en) 1997-12-15 2011-12-06 Somalogic, Inc. Methods and reagents for detecting target binding by nucleic acid ligands
US6242246B1 (en) 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
US6458543B1 (en) 1997-12-15 2002-10-01 Somalogic, Incorporated Nucleic acid ligand diagnostic biochip
US6503715B1 (en) 1997-12-15 2003-01-07 Somalogic, Inc. Nucleic acid ligand diagnostic biochip
US6544776B1 (en) 1997-12-15 2003-04-08 Somalogic, Inc. Nucleic acid ligand diagnostic biochip
US8945830B2 (en) 1997-12-15 2015-02-03 Somalogic, Inc. Multiplexed analyses of test samples
US7709192B2 (en) 1997-12-15 2010-05-04 Somalogic, Inc. Nucleic acid ligand diagnostic biochip
EP1965213A3 (en) * 1998-02-04 2009-07-15 Invitrogen Corporation Microarrays and uses therefor
EP2249155A2 (en) 2004-09-30 2010-11-10 IFOM Fondazione Istituto Firc di Oncologia Molecolare Cancer markers
EP1715341A1 (en) * 2005-04-04 2006-10-25 Micronas Holding GmbH Method and apparatus for determining the concentrations of at least 2 ligands.
US7598044B2 (en) 2005-04-04 2009-10-06 Micronas Holding Gmbh Procedure and device for determining the concentrations of at least two ligands
US10422794B2 (en) 2006-01-17 2019-09-24 Somalogic, Inc. Multiplexed analyses of test samples
US10648972B2 (en) 2006-01-17 2020-05-12 Somalogic, Inc. Multiplexed analyses of test samples
US7855054B2 (en) 2007-01-16 2010-12-21 Somalogic, Inc. Multiplexed analyses of test samples
US9404919B2 (en) 2007-01-16 2016-08-02 Somalogic, Inc. Multiplexed analyses of test samples
US9910034B2 (en) 2007-11-06 2018-03-06 Ambergen, Inc. Methods and compositions for phototransfer
US10088474B2 (en) 2007-11-06 2018-10-02 Ambergen, Inc. Methods and compositions for phototransfer
WO2012042213A1 (en) 2010-09-29 2012-04-05 Bath Ventures Novel interaction between staphylococcus aureus sbi and c3d proteins

Also Published As

Publication number Publication date
DE69410636D1 (en) 1998-07-02
EP0736176A1 (en) 1996-10-09
JP3391459B2 (en) 2003-03-31
US5837551A (en) 1998-11-17
JPH09510539A (en) 1997-10-21
GB9326450D0 (en) 1994-02-23
EP0736176B1 (en) 1998-05-27
AU1321895A (en) 1995-07-17
DE69410636T2 (en) 1998-11-26
ES2119376T3 (en) 1998-10-01

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